ApoLive-Glo Multiplex Assay G6411 from Promega

Product Specs
ItemApoLive-Glo Multiplex Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG6411
Quantity5 x 10ml
Molecular Weight~61kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Inhibiting ADAM17 enhances the efficacy of olaparib in ovarian cancer spheroidsScientific ReportsNovember 6, 2024Christoph Rogmans, Jan Dittrich, Emily Hamm, Jörg Paul Weimer, David Holthaus, Norbert Arnold, Inken Flörkemeier, Nicolai Maass, Peer Jansen, Astrid Dempfle, et al.200 µM. Read-out was performed 72 h after treatment. The Multiplex Assay ApoLive-Glo System (Promega #G6411, Fitchburg, USA), which measures cell viability and caspase 3/7 activity, was used as indicated in the

(2) Inhibition of ADAM17 increases the cytotoxic effect of cisplatin in cervical spheroids and organoidsFrontiers in OncologySeptember 2, 2024David Holthaus, Christoph Rogmans, Ina Gursinski, Alvaro Quevedo-Olmos, Marzieh Ehsani, Mandy Mangler, Inken Flörkemeier, Jörg P. Weimer, Thomas F. Meyer, Nicolai Maass, et al.caspase/viability assay was performed as described before (5) using the Multiplex Assay ApoLive-Glo (Promega, G6411) kit. For spheroids and organoids, the CellTiter-Glo 3D Cell Viability Assay was performed following

(3) Effects of neoadjuvant zoledronate and radiation therapy on cell survival, cell cycle distribution, and clinical status in canine osteosarcomaFrontiers in Veterinary ScienceJanuary 31, 2024Carissa J. Norquest, Anita Rogic, Phyllis A. Gimotty, Charles A. Maitz, Hansjorg Rindt, Hayley L. Ashworth, Jeffrey N. Bryan, Lindsay L. Donnelly, Angela L. McCleary-Wheeler, Brian K. Flesnerplates were analyzed for viability and apoptosis using the Promega ApoLive-Glo™ Multiplex Assay (cat# G6411) in technical triplicates and biologic replicates. The ratio of caspase activity to viable cells was

(4) Neutralizing IL-16 enhances the efficacy of targeting Aurora-A therapy in colorectal cancer with high lymphocyte infiltration through restoring anti-tumor immunityCell death & diseaseJanuary 30, 2024Yang SJ, Chang ST, Chang KC, Lin BW, Chang KY, Liu YW, Lai MD, Hung LY.activity assay The caspase 3/7 activity was determined using the ApoLive-Glo™ Multiplex Assay kit (G6411, Promega) according to the manufacturer’s instructions. Briefly, cells were treated with 100 nM and

(5) Molecular weight of hyaluronic acid crosslinked into biomaterial scaffolds affects angiogenic potentialActa biomaterialiaOctober 1, 2023Karam J, Singer BJ, Miwa H, Chen LH, Maran K, Hasani M, Garza S, Onyekwere B, Yeh HC, Li S, et al.plate before viability and apoptosis were measured using the ApoLive-Glo™ Multiplex Assay (Promega, G6411). Viability is measured in this assay using glycyl-phenylalanyl-amino fluorocoumarin (GF-AFC), a cell-permeant

(6) Chemotherapy-induced release of ADAM17 bearing EV as a potential resistance mechanism in ovarian cancerJournal of extracellular vesiclesJuly 1, 2023Hugendieck G, Lettau M, Andreas S, Neumann S, Reinhardt N, Arnold P, Theilig F, Bastian L, Rogmans C, Weimer JP, et al.stimulation the relative caspase activation was determined using the Multiplex Assay ApoLive‐Glo™ (Promega #G6411) according to the manufacturer's instructions (TM325). Measurements were performed with a microplate‐reader

(7) ADAM17 Inhibition Increases the Impact of Cisplatin Treatment in Ovarian Cancer SpheroidsCancersApril 23, 2021Hedemann N, Herz A, Schiepanski JH, Dittrich J, Sebens S, Dempfle A, Feuerborn J, Rogmans C, Tribian N, Flörkemeier I, et al.enzyme-linked immunosorbent assay (ELISA) quantification. Next, the Multiplex Assay ApoLive-GloTM (Promega, #G6411), which combines detection of viable cells and caspase3/7 activity, was performed as described in the

(8) Aberrant RON and MET Co-overexpression as Novel Prognostic Biomarkers of Shortened Patient Survival and Therapeutic Targets of Tyrosine Kinase Inhibitors in Pancreatic CancerFrontiers in OncologyDecember 5, 2019Chen-Yu Hu, Xiang-Ming Xu, Bo Hong, Zhi-Gang Wu, Yun Qian, Tian-Hao Weng, Yi-Zhi Liu, Tao-Ming Tang, Ming-Hai Wang, Hang-Ping Yaotreatment (0–10 μM) was measured using an ApoLive-Glo Multiplex Assay kit (size: 5 × 10 mL, cat. no. #G6411; Promega, Madison, WI, USA) to detect caspase-3/7 activity in the cells according to the manufacturer's

(9) Proteogenomic Network Analysis of Context-Specific KRAS Signaling in Mouse-to-Human Cross-Species TranslationCell SystemsSeptember 1, 2019Douglas K. Brubaker et al.WAT054925 Tandem Mass Tag,ThermoFisher Scientific,Cat. No. A34808 ApoLive-Glo Multiplex Assay,Promega,G6411 TaqMan Universal PCR Master Mix,Applied Biosystems,4304437 High-Capacity cDNA Reverse Transcription

(10) ADAM17 inhibition enhances platinum efficiency in ovarian cancerOncotargetMarch 23, 2018Hedemann N, Rogmans C, Sebens S, Wesch D, Reichert M, Schmidt-Arras D, Oberg HH, Pecks U, van Mackelenbergh M, Weimer J, et al.into a 96-well plate (Corning Costar, #3917) to perform the Multiplex Assay ApoLive-GloTM (Promega, #G6411), which combines detection of viable cells and caspase-3/7 activity. This assay was conducted following

(11) Androgen Receptor Pathway-Independent Prostate Cancer Is Sustained through FGF SignalingCancer cellOctober 9, 2017Bluemn EG, Coleman IM, Lucas JM, Coleman RT, Hernandez-Lopez S, Tharakan R, Bianchi-Frias D, Dumpit RF, Kaipainen A, Corella AN, et al.Kit,ThermoFisher,Cat#C7026 CellTiter-Glo Luminescent Cell Viability Assay,Promega,Cat#G7570 ApoLive Glo,Promega,Cat#G6411 Deposited Data,, Raw and analyzed Expression Microarray,"Kumar et al., 2016",GEO: GSE77930 Raw and

(12) Genome-wide functional genomic and transcriptomic analyses for genes regulating sensitivity to vorinostatScientific DataDecember 1, 2014Katrina J Falkenberg, Cathryn M Gould, Ricky W Johnstone, Kaylene J Simpson0.01% solution cell culture tested (Sigma, cat # P4707)Apo-Live Glo multiplexed assay (Promega, cat # G6411)Cell Titre Fluor, extra (Promega, cat # G6082) (note: additional reagent was required due to diluting

(13) Loss of PTEN stabilizes the lipid modifying enzyme cytosolic phospholipase A₂α via AKT in prostate cancer cellsOncotargetAugust 15, 2014Vignarajan S, Xie C, Yao M, Sun Y, Simanainen U, Sved P, Liu T, Dong Q.were counterstained with hematoxyline and coverslipped. Apolive-Glo Multiplex assay (Cat #: G6410 and G6411) from Promega (Madison, WI) was used to determine the biological effect of PTEN restoration. Briefly

(14) Loss of PTEN stabilizes the lipid modifying enzyme cytosolic phospholipase A2a via AKT in prostate cancer cellsOncotargetJuly 9, 2014Soma Vignarajan, Chanlu Xie, Mu Yao, Yuting Sun, Ulla Simanainen, Paul Sved, Tao Liu, Qihan Dong

(15) A High-Throughput, Multiplex Cell Death Assay Using an RNAi Screening ApproachCold Spring Harbor ProtocolsJune 2, 2014Katrina J. Falkenberg, Darren N. Saunders, Kaylene J. Simpson(includes Caspase-Glo 3/7 substrate, Caspase-Glo 3/7 buffer, GF-AFC substrate, and assay buffer; Promega G6411) For consistency in a long-term screening project spanning weeks or months, it is important to prepare

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ApoLive-Glo Multiplex Assay

ApoLive-Glo Multiplex Assay from Promega

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ApoLive-Glo Multiplex Assay from Promega

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