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cAMP-Glo Max Assay
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cAMP-Glo Max Assay
cAMP-Glo Max Assay
cAMP-Glo Max Assay from Promega
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Citations:
(17)
Figures:
(1)
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cAMP-Glo Max Assay
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Description
The cAMP-Glo Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
cAMP-Glo Max Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V1681
Quantity
2 plates
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (17)
(1)
Non-Autoimmune Hyperthyroidism Associated With a Novel Germline Mutation (D633N) in the TSH Receptor
Case Rep Endocrinol
June 26, 2026
Sayuri Mori, Yoshinori Osaki, Hiroshi Fukazawa, Takaaki Matsuda, Yuki Murayama, Yoko Sugano, Hitoshi Iwasaki, Bryan J. Mathis, Motohiro Sekiya, Hitoshi Shimano
at 37°C. Thereafter, the cAMP content of the cell was measured with the
cAMP‐Glo Max Assay
(
Promega
,
V1681
) according to the manufacturer’s instructions. Results were presented as fold changes relative to the
(2)
Relaxin-2 drives regenerative healing and suppresses scar formation
bioRxiv
December 12, 2025
Amanda K. Williamson, Katherine M. Hohl, Jack R. Kirsch, Rosalynn M. Nazarian, Thomas P. Schaer, Daniel S. Roh, Mark W. Grinstaff
treated 96-well white opaque plate and allowed to adhere overnight. The
Promega
cAMP Glo-Max assay
(
V1681
) was used following the manufacturer protocols. RLX-2 was diluted to 13.5 μg/mL in media and a 3-fold
(3)
CB2 cannabinoid receptor-specific therapeutic antibody agonists for treatment of chemotherapy-induced peripheral neuropathy
bioRxiv
December 1, 2025
Carlos Henrique Alves Jesus, Raghavender Gopireddy, Emily Sizemore, Jonah L. Wirt, Swastik Sen, Richard Yu, Toshihiko Takeuchi, Lauren Schwimmer, Andrea G. Hohmann
collected for downstream application. 2.5
Luminescence
cAMP assay
cAMP-Glo Max assay
kit (
Promega
,
V1682
) was used to
measure
cAMP signals in RAW264.7 cells as described previously [21]. Briefly, 384 well
(4)
Transcriptomic landscape of hyperthyroidism in mice overexpressing thyroid-Stimulating hormone
iScience
January 17, 2025
Ichiro Yamauchi, Taku Sugawa, Takuro Hakata, Akira Yoshizawa, Tomoko Kita, Yo Kishimoto, Sadahito Kimura, Aya Sakurai, Daisuke Kosugi, Haruka Fujita,
et al.
740412 "FT3, FT4, TSH AccuLite VAST CLIA kit",Monobind Inc.,Cat# 7075
cAMP-Glo™ Max Assay
,
Promega
,Cat#
V1681
Nucleospin RNA Plus
kit,
Macherey-Nagel
,Cat# 740984 ReverTra Ace,TOYOBO Life Science,Cat# FSQ-201 Emerald
(5)
ZBTB21 suppresses CRE-mediated transcription to impair synaptic function in Down syndrome
Science Advances
July 5, 2024
Muzhen Qiao, Qianwen Huang, Xin Wang, Jiahuai Han
assay
was performed following the protocol described previously (59), using the
cAMP-Glo Max Assay
(
V1681
,
Promega
). The standard
assay
protocol was followed using the abovementioned cAMP levels
assay
kit. PKA
(6)
White adipocytes in subcutaneous fat depots require KLF15 for maintenance in preclinical models
J Clin Invest
July 1, 2024
Liang Li, Brian J. Feldman
Scientific). Measurements of cAMP content.
Adipocytes
infected with Ad-Control or Ad-Cre were used in cAMP-Glo Max
Assays
(
V1681
,
Promega
) according to the manufacturer’s protocol.
Luminescence
is inversely proportional to cAMP levels
(7)
White adipocytes in subcutaneous fat depots require KLF15 for maintenance in preclinical models
Journal of Clinical Investigation
July 1, 2024
Liang Li, Brian J. Feldman
Scientific). Measurements of cAMP content.
Adipocytes
infected with Ad-Control or Ad-Cre were used in cAMP-Glo Max
Assays
(
V1681
,
Promega
) according to the manufacturer’s protocol.
Luminescence
is inversely proportional to cAMP levels
(8)
Sex-dependent regulation of mucin gene transcription and airway secretion and mechanics following intra-airway IL-13 in mice with conditional loss of club cell Creb1
Frontiers in Physiology
April 22, 2024
Mariana Sponchiado, Amy Fagan, Luz Mata, Angelina L. Bonilla, Pedro Trevizan-Baú, Sreekala Prabhakaran, Leah R. Reznikov
about the cAMP assay are in below section. Measurement of cAMP The
cAMP-Glo Max Assay
(
Promega
, cat. #
V1681
) was followed according to the manufacturer’s instructions and as previously described by our lab (Sponchiado
(9)
Arrestin-dependent nuclear export of phosphodiesterase 4D promotes GPCR-induced nuclear cAMP signaling required for learning and memory
Science Signaling
March 28, 2023
Joseph M. Martinez, Ao Shen, Bing Xu, Aleksandra Jovanovic, Josephine de Chabot, Jin Zhang, Yang K. Xiang
aged 70–130 days were solubilized according to the manufacturer’s protocols (
Promega
cAMP Glo Max,
V1681
, Madison, WI). cAMP levels were determined from a dilution curve using a microplate reader (
CLARIOStarPlus
,
(10)
Club cell CREB regulates the goblet cell transcriptional network and pro-mucin effects of IL-1B
Frontiers in physiology
January 1, 2023
Sponchiado M, Bonilla AL, Mata L, Jasso-Johnson K, Liao YJ, Fagan A, Moncada V, Reznikov LR.
and stored at −80°C until RNA isolation. Measurement of cAMP The
cAMP-Glo Max Assay
(
Promega
, cat. #
V1681
) was followed according to the manufacturer’s instructions. Briefly, H322 cells were seeded at a density
(11)
Differential Effects of a Novel Opioid Ligand UTA1003 on Antinociceptive Tolerance and Motor Behaviour
Pharmaceuticals (Basel)
June 24, 2022
Alok K. Paul, Krystel L. Woolley, Mohammed Rahmatullah, Polrat Wilairatana, Jason A. Smith, Nuri Gueven, Nikolas Dietis
measured effects are associated with specific opioid receptors. The Promega cAMP-GloTM Max
assay
kit (
V1681
,
Promega Corporation
, Madison, WI, USA) was used in this study. The commercial kit contained cAMP 100
(12)
Osteocalcin Alleviates Lipopolysaccharide-Induced Acute Inflammation via Activation of GPR37 in Macrophages
Biomedicines
April 27, 2022
Qian Z, Liu C, Li H, Yang H, Wu J, Liu J, Li Y, Chen X, Xu J, Li X.
change of intracellular cAMP concentration was measured using the
cAMP-Glo Max Assay
Kit (
Promega
, Cat#
V1682
) following the manufacturer’s instructions. All the experiments were repeated at least four times in
(13)
Critical cysteines in the functional interaction of adenylyl cyclase isoform 6 with Gαs
FASEB bioAdvances
March 1, 2022
Bhagirath AY, Bhatia V, Medapati MR, Singh N, Hinton M, Chelikani P, Dakshinamurti S.
β‐d‐maltoside (DM) was purchased from Sigma‐Aldrich (Cat #D4641). The
CAMP‐Glo Max assay
kit (Cat #
V1681
) was obtained from
Promega
. Gαi‐based cAMP homogeneous time‐resolved
fluorescence
(
HTRF
) kit was obtained
(14)
The secondary messenger ppGpp interferes with cAMP-CRP regulon by promoting CRP acetylation in Escherichia coli
PLOS ONE
October 27, 2021
Chunghwan Ro, Michael Cashel, Llorenç Fernández-Coll
adenosine monophosphate) were determined through the usage of
cAMP-Glo™ Max Assay
kit (
Promega
, cat. #
V1681
). Briefly, cells were grown by duplicate in minimal media (50 ml of media in 250 ml flasks) with aeration
(15)
Osteocalcin attenuates oligodendrocyte differentiation and myelination via GPR37 signaling in the mouse brain
Science advances
October 22, 2021
Qian Z, Li H, Yang H, Yang Q, Lu Z, Wang L, Chen Y, Li X.
OCN
for 30 min, the cAMP level was analyzed using the
cAMP-Glo Max Assay
Kit (
Promega
, catalog no.
V1682
) following the manufacturer’s instructions. For cAMP quantification in OL, primary
rat
OPCs were transduced
(16)
Reciprocal signalling by Notch-Collagen V-CALCR retains muscle stem cells in their niche
Nature
May 1, 2018
Baghdadi MB, Castel D, Machado L, Fukada SI, Birk DE, Relaix F, Tajbakhsh S, Mourikis P.
(0.1U/ml) for 3h. The amount of intracellular cAMP was measured using
cAMP-Glo Max Assay
(
Promega
,
V1681
) following the manufacturer’s protocol.
Luminescence
was quantified with
FLUOstar OPTIMA
(
BMG Labtech
).
(17)
GM1 ganglioside-independent intoxication by Cholera toxin
PLoS pathogens
February 1, 2018
Cervin J, Wands AM, Casselbrant A, Wu H, Krishnamurthy S, Cvjetkovic A, Estelius J, Dedic B, Sethi A, Wallom KL,
et al
.
the CT dilution buffer. Accumulated cAMP was measured using the
cAMP-Glo Max assay
(
Promega
, Cat. No.
V1681
) according to the manufacturer’s instructions.
Luminescence
was determined with a Synergy Neo microplate
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