Nano-Glo Luciferase Assay N1150 from Promega

Product Specs
ItemNano-Glo Luciferase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1150
Quantity10 x 100ml
Molecular Weight~67kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translationNucleic Acids ResApril 30, 2026Dorthy Fang, John M Babich, Ryan Stanton, Isaac W Vock, Kyrillos Abdallah, Mingyi Zhu, Raj Letchuman, Richard Li, Matthew D Simon, Wendy V Gilbert, et al.at 30°C. Luciferase activity was then measured using the Nano-Glo Luciferase Assay System (Promega, N1150). Targeted modification detection by ultraMarathonRT-PCR RNA (100 ng for in vitro-transcribed RNAs)

(2) Systemic Inflammation Modulates Clearance and Drives Extra-Hepatic Distribution of Extracellular VesiclesJ Extracell VesiclesApril 7, 2026Svetlana Pavlova, Doste R. Mamand, André Görgens, Antje M. Zickler, Wenyi Zheng, Xiuming Liang, Oscar P. B. Wiklander, Manuchehr Abedi-Valugerdi, Elien Van Wonterghem, Junhua Xie, et al.GloMax 96 Microplate Luminometer (Promega, USA) was used to inject the substrate Nano‐Glo (Promega; N1130) at 25 µL per well automatically, and luminescence intensity was recorded on a multimode plate reader. The

(3) An allosteric inhibitor of the Zika virus NS2B-NS3 protease with oral efficacy in mouse modelsNat CommunFebruary 10, 2026Jesus M. Ontoria, Esther Torrente, Antonino Missineo, Cristina Alli, Rita Graziani, Silvia Conti, Monica Bisbocci, Antonio Quotadamo, Federica Ferrigno, Alessandra Corio, et al.incubation at 37 °C with 5% CO2, the NanoLuc signal was revealed using the Nano-Glo reagent (Promega, Cat. #N1150), and cell viability was assessed using CellTiter-Glo (Promega, Cat. #G7573). Luminescence was measured

(4) Nanoluciferase-CD63 labeling reveals extracellular vesicle kinetics in a mouse intraductal model of ductal carcinoma in situBreast Cancer ResearchJanuary 30, 2026Cole Hladik, Sugantha Priya Elayapillai, Samrita Dogra, Matthew Bruns, Elizabeth A. Wellberg, Alexander Filatenkov, Fariba Behbod, Bethany N. Hannafonassay Cellular and secreted CD63-NLuc were quantified using the Nano-Glo® Luciferase Assay System (Promega, N1130). Total protein was extracted from tissue lysates using T-PER (Thermo, 78510) supplemented with protease

(5) Targeting the ERα DBD-LBD Interface with Mitoxantrone Disrupts Receptor Function through Proteasomal DegradationMolecular cancer therapeuticsJanuary 2, 2026Wang H, Luo Y, Artham S, Wang Q, Peng Y, Yun Z, Li X, Wu C, Liu Z, Weber-Bonk KL, et al.Nano-Glo Luciferase assay substrate (Promega, # N1120) was added per well in Nano-Glo LCS dilution buffer (Promega, #N1150). Following a 5-minute room temperature incubation, baseline luminescence was measured using a FLUOstar Omega

(6) Overcoming matrix effects in AAV neutralization assays with a constant serum concentration approachGene TherapyJanuary 1, 2026Beatrix Kovács, Viktória Szabó, Domonkos Horváth, Attila Balázs Dobos, Zoltán Zsolt Nagy, Wim Vanduffel, Zsuzsanna Szemlaky, Áron Szepesi, István Ulbert, Balázs Rózsa, et al.luminescence measurement, half of the medium was removed from each well and replaced with Nano-Glo assay reagent (Promega, N1130, Madison, WI, USA), prepared according to the manufacturer’s instructions. Luminescence was measured

(7) High throughput screening assay for the identification of ATF4 and TFEB activating compoundsAutophagy ReportsDecember 31, 2025Daniel J. Pfau, Ruslana Brykincubated for 6.5 to 7.5 hours and luminescence measured using the Nano-Glo Luciferase Assay System (N1130) from Promega as per the manufacturer’s instructions. Signal was detected on a SpectraMax iD5 microplate

(8) A neutralizing human antibody induces movement of the HCoV-229E receptor binding domainCommunications BiologyDecember 31, 2025Mera F. Liccione, Trevor Scobey, Heather M. Froggatt, Camryn Pajon, Boyd L. Yount, Qi Yin, Taylor N. Spence, Emmanuel B. Walter, Kevin O. Saunders, Robert J. Edwards, et al.and after 43 h, viral replication was measured via Nano-Glo Luciferase Assay System (Promega, Cat.#: N1150) on a GloMax Explorer Multimode Microplate Reader (Promega, Madison, WI). Percent neutralization was

(9) Co-transcriptional modifications of 2′-hydroxyls on synthetic mRNA enhance ribonuclease resistance and lipid nanoparticle thermostabilityMolecular Therapy Nucleic AcidsDecember 9, 2025Kohki Yamada, Yun-Chieh Sung, Tsung-Ying Lee, Daniel J. Siegwartadded at a dose of 20 ng per well. After a 24-h incubation, the Nano-Glo luciferase assay (cat. no. N1130, Promega) and CellTiter-Fluor cell viability assay (cat. no. N7120, Promega) were performed to measure

(10) Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translationbioRxivDecember 8, 2025Dorthy Fang, John M. Babich, Ryan Stanton, Isaac W. Vock, Kyrillos Abdallah, Mingyi Zhu, Richard Li, Matthew D. Simon, Wendy V. Gilbert, Sigrid Nachtergaeleat 30°C. Luciferase activity was then measured using the Nano-Glo Luciferase Assay System (Promega, N1150). Targeted modification detection by uMRT-PCR RNA (100 ng for in vitro-transcribed RNAs) was reverse

(11) Novel tricycle expanded purine nucleosides with pan-viral activityBioorganic & Medicinal ChemistryDecember 1, 2025Céline Martin-Ghoteimi, Kyle A. Davis, Rayane Ghoteimi, Meareg Amare, Meghan V. Diefenbacher, Christianna Kutz, Jessica L. Smith, Laura J. Stevens, Egor Tchesnokov, Simon M. Walker, et al.post-infection, luciferase expression was quantified using the NanoGlo Luciferase Assay System (Promega #N1130) according to manufacturer’s instructions. EC50 values were determined by fitting normalized data points

(12) Design of facilitated dissociation enables timing of cytokine signallingNatureNovember 13, 2025Adam J. Broerman, Christoph Pollmann, Yang Zhao, Mauriz A. Lichtenstein, Mark D. Jackson, Maxx H. Tessmer, Won Hee Ryu, Masato Ogishi, Mohamad H. Abedi, Danny D. Sahtoe, et al.72 μl of this mixture was added to two wells, then 8 μl of 1/10-diluted Nano-Glo substrate (Promega N1130) and either 10 μM effector and 200 μM target or just 200 μM target was added to these wells using a

(13) The essential host genome for Cryptosporidium survival exposes metabolic dependencies that can be leveraged for treatmentCellOctober 16, 2025N. Bishara Bishara Marzook, Ok-Ryul Song, Lotta Baumgärtel, Netanya Bernitz, Tapoka T. Mkandawire, Lucy C. Watson, Vanessa Nunes, Scott Warchal, James I. MacRae, Michael Howell, et al.Red,Invitrogen,Cat# C10422 GSH/GSSG-Glo™ assay,Promega,Cat# V6611 NanoGlo Luciferase kit,Promega,Cat# N1150 RNeasy Mini kit,Qiagen,Cat# 74104 Watchmaker mRNA Library Prep kit,Watchmaker Genomics,Cat# 7BK0001 Deposited

(14) Cerium dioxide nanoparticles coated with benzo[a]pyrene modify aryl hydrocarbon receptor activity, trophoblast differentiation and mitochondrial network phenotype in human placentaPart Fibre ToxicolOctober 14, 2025Séverine A. Degrelle, Gaëlle Deval, Arnaud Tête, Claire Mikolajczak, Frank Giton, Marie-Léone Vignaud, Sonja Boland, Céline J. Guigon, Xavier Coumoul, Amal Zerrad-Saadi, et al.NanoLuc® luciferase activity in supernatants was quantified using the Nano-Glo® Luciferase Assay (#N1130 Promega Corporation), according to the manufacturer’s instructions. Measurements of luminescence and

(15) Exploration of 4′-fluoro fleximer nucleoside analogues as potential broad-spectrum antiviral agentsBioorganic & Medicinal ChemistryOctober 1, 2025Kyle A. Davis, Jason K. Smith, Jessica L. Smith, Meareg Amare, Meghan V. Diefenbacher, Peter Halfmann, Timothy P. Sheahan, Alec J. Hirsch, Katherine L. Seley-Radtkepost-infection, luciferase expression was quantified using the NanoGlo Luciferase Assay System (Promega #N1130) according to manufacturer’s instructions. EC50 values were determined by fitting normalized data points

(16) A human H5N1 influenza virus expressing bioluminescence for evaluating viral infection and identifying therapeutic interventionsiScienceSeptember 19, 2025Ramya S. Barre, Ruby A. Escobedo, Esteban M. Castro, Michal Gazi, Joshua D. Castro, Anastasija Cupic, Mahmoud Bayoumi, Nathaniel Jackson, Chengin Ye, Aitor Nogales, et al.HRP substrate kit,Vector Laboratories,SK-4105 Nano-Glo® Luciferase Assay System,Promega,Catalog # N1150 ,, Deposited data,Deposited data,Deposited data ,, Raw sequence reads,NCBI BioProjects,PRJNA1273115 Nanopore sequencing

(17) Real-time monitoring with iTLR4 assay identifies ligand-dependent TLR4-TLR4 conformational dynamicsFrontiers in ImmunologySeptember 1, 2025Sanam Mustafa, Samuel G. Evans, Mark R. HutchinsonEU/mL in PBS before preparation of final working concentration. Nano-Glo® Luciferase assay substrate (N1130) was purchased from Promega (Aus). Cell Signalling Technology® anti-mouse NFkB p65 (6956S), anti-rabbit

(18) Optogenetic‐Controlled iPSC‐Based Vaccines for Prophylactic and Therapeutic Tumor Suppression in MiceAdvanced ScienceSeptember 1, 2025Longliang Qiao, Lingxue Niu, Zhihao Wang, Di Dai, Shasha Tang, Xiaoding Ma, Zhenqiang Deng, Guiling Yu, Yang Zhou, Tao Yan, et al.Reporter Assay The NanoLuc activities were quantified using Nano‐Glo Luciferase Assay (catalog no. N1130, Promega) according to the manufacturer's instructions. Nano‐Glo Luciferase Assay reagent was prepared

(19) Clonal expansion and diversification of germinal center and memory B cell responses to booster immunization in primatesCell ReportsAugust 26, 2025Lachlan P. Deimel, Yoshiaki Nishimura, Gabriela S. Silva Silva Santos, Viren A. Baharani, Brianna Hernandez, Thiago Y. Oliveira, Andrew J. MacLean, Marie Canis, Sadman Shawraz, Anna Gazumyan, et al.Flow cell,Illumina,N/A BirA500 reaction kit,Avidity,BirA500 Nano-Glo Luciferase Assay System,Promega,N1150 Luciferase Cell Culture Lysis 5X Reagent,Promega,E1531 SARS-CoV-2 Wu-1-Spike,In-house,N/A SARS-CoV-2

(20) CoreTIA: a modular, statistically robust transduction inhibition assay for AAV neutralizationFrontiers in ImmunologyAugust 20, 2025Beatrix Kovács, Fanni Somogyi, Viktória Szabó, Zoltán Zsolt Nagy, István Hernádi, Ferenc Mátyás, Wim Vanduffel, Zsuzsanna Szemlaky, Balázs Rózsa, István Ulbert, et al.15250061)Poly-ʟ-Lysine Hydrobromide (Sigma-Aldrich, P4707)Nano-Glo® Luciferase Assay Reagent (Promega, N1130)Anti-AAV9 Intact Particle Mouse Monoclonal (ADK9), (Progen, 690162)Serum to test from patient or donor

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Nano-Glo Luciferase Assay

Nano-Glo Luciferase Assay from Promega

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Nano-Glo Luciferase Assay from Promega

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