Description
Fewer resources used, less hazardous, less waste
This kit is designed for use with the
Power SYBRGreen (Cat. Nos. 4402953, 4402954, and 4402955) and Fast SYBRGreen Cells-to-C
T™ Kits (Cat. Nos. 4402956 and 4402957). Sufficient reagents are provided for 100 reactions. It includes Xeno™ RNA Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, and a PCR primer set for the Xeno RNA Control target. It also includes a PCR primer set for the highly expressed endogenous control gene β-actin (ACTB). Together these reagents provide positive controls for reverse transcription and SYBRGreen-based real-time PCR, and can indicate the presence of any reverse transcription or PCR inhibitors. Real-time RT-PCR with SYBRACTB Primers can also provide an endogenous control for sample normalization, as well as to confirm sufficient cell input in experiments with samples consisting of more than 100 cells per lysis.
- Polymerase: AmpliTaq Gold™ DNA Polymerase
- Purity/Quality Grade: UP (Ultra Pure)
PCR and Real-Time PCR, Real Time PCR (qPCR), Real-Time PCR Directly from Samples