PureLink RNA Mini Kit 12183020 from Thermo Fisher Scientific

Product Specs
ItemPureLink RNA Mini Kit
CompanyThermo Fisher Scientific
Price $109.00 Inquire
Catalog Number12183020
Quantity10 preps
SystemPureLink™
ApplicationsRT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Sample TypeCell and tissue samples
CompatibilityNot High Throughput-Compatible
YieldUp to 350 µg
SourceBacterial, Plant, Fungal, Cell/Tissue, Blood, Plasma/Serum
Nucleic Acid TypeTotal RNA
Assay Time20 min. (After sample homogenization)
FormatColumn-based

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) In vitro plasticity between ureteric epithelial and distal nephron identity and maturity is controlled by extracellular signalsDevelopmental BiologyMay 1, 2026Pamela Kairath, Pei Xuan Er, Sean Wilson, Irene Ghobrial, Jessica M. Vanslambrouck, Yi-Hsien Yi-Hsien, Pruett-Miller Shondra Pruett-Miller, Sanjay Jain, Melissa H. LittleZeiss). Quantitative RT-PCR analysis: Total RNA was extracted from cells using Purelink RNA Mini Kit (Cat# 12183020, ThermoFisher Scientific) as per manufacturer’s instructions. RNA was quantified using a NanoDrop 1000

(2) Induction of adipocyte thermogenic program by Rho-associated coiled-coil containing kinase 2iScienceApril 17, 2026Haw-Chih Tai, Xiaoou Li, Christina J. Park, Altayeb Alshaikh, Brian Yu, Daiqing Chen, Phetcharat Chen, James K. Liaocultured cells using TRIzol (Invitrogen; 15596018) combined with a PureLink RNA Mini Kit (Invitrogen; 12183020). Complementary DNA was reverse transcribed from total RNA with an iScript cDNA Synthesis Kit (Bio-Rad;

(3) Time Course Characterization of Protective Immune Responses Following BCG Vaccination in BALB/c MicePathogensApril 6, 2026Hee Ho Kim, Kwangwook Kim, Min Jung Kim, Ye Jin Yang, Eun Bee Choi, Ji Woong Heo, Seo Young Moon, Heeji Lim, Yookyoung Lee, In-Ohk Ouh, et al.the schedule shown in Figure 1, using an RNase mini kit (Thermo Fisher Scientific, Waltham, MA, USA, 12183020) and TRIzol™ (Thermo Fisher Scientific, Waltham, MA, USA, 10296028). Genomic DNA was removed using DNase I

(4) The RNA-binding protein NOVA-1 regulates circRNA expression, alternative splicing, and aging in Caenorhabditis elegansG3: Genes, Genomes, GeneticsApril 1, 2026Emmanuel Adeyemi, Hussam Z Alshareef, Jaffar M Bhat, Pedro Miura, Alexander M van der LindenPurelink RNA mini-kit, followed by a DNAse I treatment following the manufacturer's protocol (Ambion, Cat #12183020). RNA was quantified by a Nanodrop. Bioanalyzer or tapestation (Agilent) was used for qualification

(5) Exploring roles of neuroglobin in oxygen homeostasis and neurodevelopment based on human cerebral organoid modelsiScienceMarch 20, 2026Yuan-Hsuan Liu, Meng-Ting Chung, Hsi-Chieh Lin, Hsin-Lei Lin, Hsuan Lin, Ya-Jen Cheng, Hsiao-Mei Wu, Tzyy-Wen Chiou, Yi-Chung Tung(MEA) system,Alpha MED Scientific,MED64 system PureLink™ RNA Mini Kit,Thermo Fisher Scientific,Cat# 12183020 ToolsQuant II Fast RT Kit,BIOTOOLS,Cat# KRT-BA06-2 TOOLS 2xSYBR qPCR Mix Kit,BIOTOOLS,Cat# FPT-BB05 ,, Deposited

(6) Longitudinal plasma interleukin-6 and post-stroke cognitive outcomes: The Stroke-IMPaCT studyAlzheimers DementMarch 10, 2026Natasha S. Carmichael, Harry R. Deijnen, Siew Yan Wong, Thomas O. Williams, Evangelos Kontopantelis, Luke Cowie, Eileen Jones, Lauren Drag, Marion S. Buckwalter, John R. Grainger, et al.analyses Total RNA was extracted from PBMCs using the PureLink RNA Mini Kit (Cat. Nos. 12183018A, 12183020, 12183025; ThermoFisher Scientific), following the manufacturer's protocol. An on‐column DNase digestion

(7) Microcephaly-associated protein WDR62 supports purine metabolism by interacting with co-chaperone BAG2The EMBO JournalMarch 5, 2026Matthew J Morris, Yvonne Y Yeap, Jonathon R Edwards, Chi Chen, Annalisa Paolino, Sebastian G B Furness, S Sean Millard, Julia K Pagan, Laura R Fenlon, Dominic C H Nganalysis Total RNA was extracted from WT and WDR62 KO AD293 cells using the PureLink™ RNA Mini Kit (Invitrogen, #12183020). 1 µg total RNA was used to perform reverse transcription using SuperScript™ III Reverse Transcriptase

(8) CD8 + T cells are primed by cDC1 and exacerbate tau-mediated neurodegenerationbioRxivMarch 1, 2026Hao Hu, Peter Bor-Chian Lin, Carisa Zeng, Prabal Sharma, Yongyi Li, Hong Jiang, Jonathan Nulman, Ray A. Ohara, Tong Wu, Shasha Li, et al.microglia cells sorted from the brain by FACS was isolated by using the PureLink RNA mini kit (Invitrogen, 12183020) and PureLink DNase (Invitrogen, 12185010) according to the manufacturer’s protocol. 50 nanograms of

(9) SMCHD1 loss re-wires MYOD1 enhancer nexuses and chromatin accessibility landscapes in muscle cellsbioRxivFebruary 25, 2026Zhijun Huang, Wei Cui, Adam Klaiss, Gerd P. Pfeiferprevious study76. The total RNA was purified from LHCN-M2 cells using the PureLink RNA Mini Kit (Ambion, 12183020) according to the manufacturer’s protocol. Total RNA QC was verified by Agilent 2100 Bioanalyzer (Agilent

(10) Making the best of a sticky situation: Infection-mediated endothelial activation promotes new interactions with adhesins of the host-adapted Lyme disease spirochetebioRxivFebruary 24, 2026Daiana Alvarez Alvarez Olmedo, Xi Tan, Colton Scott, Aleksandra Shcherbakova, George Chaconas(ThermoFisher, 15596026) was added and total RNA was purified using the PureLink RNA Mini Kit (Ambion, 12183020) including the On-column PureLink DNAse Treatment step. RNA integrity and concentration was checked

(11) Fatty acids promote uncoupled respiration via ATP/ADP carriers in white adipocytesNature MetabolismFebruary 23, 2026Maryam Ahmadian, A. Melisa Aksu, Preetveer Dhillon, Zane J. Zerbel, Yosip Kelemen, Oluwafemi Gbayisomore, Nicolás Gómez-Banoy, Serena J. Chen, Shannon M. Reillybrown adipose tissue were performed using the PureLink RNA Mini Kit (Thermo Fisher Scientific catalogue number 12183020). We used SuperScript IV VILO Master Mix Synthesis System for reverse-transcription PCR (Thermo Fisher Scientific

(12) Chronic kidney disease induces distinct alterations of macrophage lipid metabolism in a mouse model of atherosclerosisJournal of Lipid ResearchFebruary 1, 2026Keith Saum, Xinyi Liu, Thekkelnaycke Rajendiran, Lixia Zeng, Pradeep Kayampilly, Jaeman Byun, Farsad Afshinnia, Subramaniam PennathurRNA was extracted from cell pellets using the Purelink RNA Mini Kit (Thermo Fisher Scientific, cat # 12183020) with on-column DNAse treatment. RNA concentration and purity were tested using a NanoDrop 2000 spectrophotometer.

(13) An ultrasmall core–shell silica nanoparticle improves antitumour immunity and survival by remodelling suppressive melanoma microenvironmentsNature NanotechnologyFebruary 1, 2026Gabriel De Leon, Li Zhang, Nabil A. Siddiqui, Nada Naguib, Feng Chen, Reethi Padmanabhan, Tuo Zhang, Sebastien Monette, Fabio Socciarelli, Rachel Lee, et al.indicated time points and RNA extracted using the Purelink RNA minikit (ThermoFisher catalogue number 12183020) per the manufacturer’s instructions. DNase removal was performed on a column per the manufacturer’s

(14) Investigating circulating expression profile for H19, MEG3, and MIAT long noncoding RNAs with miR-135a and miR-29a in chronic kidney disease and renal hemodialysis patients: interrelations with serum sclerostinFrontiers in Molecular BiosciencesJanuary 23, 2026Marwa A. Dahpy, Marwa K. Khairallah, Rania Naguib, Mona A. Khalil, Salwa Seif Eldin, Marwa A. Sabet, Amira A. Kamelusing qRT-PCR Total RNAs were extracted using the PureLink™ RNA Mini Kit (Invitrogen, Catalog No. 12183020), and the NanoDrop spectrophotometer was used to measure their concentration. The polyadenylate polymerase

(15) Optimization and Characterization of SHIP1 Ligands for Cellular Target Engagement and Activity in Alzheimer’s Disease ModelsbioRxivJanuary 5, 2026Cynthia D. Jesudason, Claudia Rangel-Barajas, Colin J. Beach, Daniel E. Beck, Isaac H. Caballero-Floran, W. Brent Clayton, Lais Da Silva, Juliane C. David, Suzanne Doolen, Alexandra N. Faulkner, et al.RNA STAT-60 (Tel-Test Inc., CS-502). RNA was isolated using the PureLink RNA mini kit (Invitrogen, 12183020) and PureLink DNase (Invitrogen, 12185010) according to the manufacturer’s protocol. RNA quality and

(16) HIV disrupts the lung molecular clock, leading to lung inflammation and features of emphysemaCommun BiolJanuary 5, 2026Kingshuk Panda, Joyce Yao-chun Peng, Maria J. Santiago, Md. Sohanur Rahman, Sadiya Bi Shaikh, Gagandeep Kaur, Md Imam Faizan, Khursheed Ul Islam, Ting Wang, Stephen M. Black, et al.(qRT-PCR) Total RNA was extracted from cells using the PureLink RNA Mini Kit (Invitrogen, cat. no. 12183020). RNA purity and concentration were assessed with a microspot RNA reader (Synergy HT Multi-Mode Microplate

(17) Astrocytic Chromatin Remodeler ATRX Gates Hippocampal Memory Consolidation Through Metabolic and Synaptic RegulationGliaJanuary 1, 2026Miguel A. Pena‐Ortiz, Julia K. Sunstrum, Alireza Ghahramani, Haley McConkey, Vanessa Dumeaux, Wataru Inoue, Nathalie G. BérubéLibrary Preparation RNA was extracted from mouse hippocampus using the PureLink RNA Mini Kit (cat# 12183020, Thermo Fisher Scientific) with DNAse treatment (Cat# 12185010, Thermo Fisher Scientific). PolyA+ libraries

(18) The Receptor for Advanced Glycation End-Products (RAGE) Regulates Cell Adhesion Through Upregulation of ITGA8CellsNovember 17, 2025Swetha Thiyagarajan, Estelle Leclerc, Stefan W. Vettercell RNA was extracted using the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, cat# 12183020, Waltham, Massachusetts, USA) according to the manufacturer’s protocol. The concentration of the RNA

(19) The repressor Capicua is a barrier to lung tumor development driven by Kras/Trp53 mutationsEMBO Molecular MedicineNovember 11, 2025Irene Ballesteros-González, Iván Hernández-Navas, Oksana Brehey, Carmen G Lechuga, Marina Salmón, Morena Scotece, Ricardo Velasco-Vicente, Alejandra A Flores-Gómez, Antonio Cebriá, Lucía Simón-Carrasco, et al.reagents" Trametinib,MedChemExpress,HY-10999 PFK15,Sigma-Aldrich,SML1009 TX-1123,Sigma-Aldrich,655200 RNeasy Mini Kit,Qiagen,74104 PureLink RNA Mini Kit,Invitrogen,12183020 NZY First-Strand cDNA Synthesis kit,NZYTech,MB12501 PowerTrack SYBR Green PCR Master Mix,Applied Biosystems,16525231 Cell

(20) Central thyroid hormone receptor-beta: Sensitivity to alcohol and a role in regulating alcohol drinkingHormones and BehaviorNovember 1, 2025Michael C. Johnson, Michelle A. Nipper, Kelly M. Abshire, Jessica E. Rehmann, Jonathan A. Zweig, Theresa N. Vu, Mandee A. Bell, Tapasree Banerji, Thomas S. Scanlan, Andrey E. Ryabinin, et al.from frozen tissue punches using a modified RNA extraction protocol (Walker et al. 2022; Invitrogen, #12183020). RNA concentrations were determined on a Nanodrop spectrophotometer and RNA quality was assessed using

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PureLink RNA Mini Kit from Thermo Fisher Scientific

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PureLink RNA Mini Kit from Thermo Fisher Scientific

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