Human Tissue factor pathway inhibitor,TFPI ELISA Kit CSB-E08260h from CUSABIO Technology LLC

Product Specs
ItemHuman Tissue factor pathway inhibitor,TFPI ELISA Kit
CompanyCUSABIO Technology LLC
Price $450.00Supplier Page View Company Product Page
Catalog NumberCSB-E08260h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Nametissue factor pathway inhibitor
NCBI Gene AliasesEPI, LACI, TFI, TFPI1
Research AreaCardiovascular
Assay Time1-5h
Detection Targettissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Volume50-100ul
Sensitivity0.293 ng/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range0.782 ng/mL-50 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TFPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TFPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TFPI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TFPI bound in the initial step. The color development is stopped and the intensity of the color is measured.

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