Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit CSB-E07949h from CUSABIO Technology LLC

Product Specs
ItemHuman intercellular adhesion molecule 3,ICAM-3 ELISA Kit
CompanyCUSABIO Technology LLC
Price $468.00Supplier Page View Company Product Page
Catalog NumberCSB-E07949h
NCBI Full Gene Nameintercellular adhesion molecule 3
NCBI Gene AliasesCD50, CDW50, ICAM-R
Research AreaImmunology
MethodELISA Kit
ApplicationsELISA
Assay Time1-5h
Volume50-100ul
Sensitivity7.8 pg/ml
Sample Typeserum, plasma,tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range31.25 pg/ml - 2000 pg/ml
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ICAM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ICAM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ICAM3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ICAM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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