Human soluble CD23(sCD23) ELISA Kit CSB-E14264h from CUSABIO Technology LLC

Product Specs
ItemHuman soluble CD23(sCD23) ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-E14264h
Quantity96T
Detection Range0.312 ng/mL-20 ng/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates
Sensitivity0.078 ng/mL
Volume50-100ul
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameFc epsilon receptor II
NCBI Gene AliasesBLAST-2, CD23, CD23A, CLEC4J, FCE2, FCErII, IGEBF
Research AreaImmunology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FCER2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FCER2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCER2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCER2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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