Cell Counting Kit 30 dual-chambered slides 60 counts with trypan blue #1450003 from Bio-Rad

Use these dual-chamber slides for fast, highly reproducible cell counts using the TC10™ or TC20™ Automated Cell Counters. The kit contains filter-sterilized 0.4% (w/v) trypan blue in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution.

• Counting parameters– range of 5 x 10<su4</su– 1 x 10<su7</su cells/ml and 6– 50 µm cell diameter
• Each chamber only requires 10 µl of suspended cells
• Volume counted in each chamber is 0.4 µl (equivalent to four 1 x 1 mm squares of a manual hemocytometer)
• Both chambers can be loaded prior to insertion
• Chambers labeled A and B, with arrow next to chamber indicating direction of slide insertion
• Can be used with or without trypan blue

The counting slides are disposable plastic slides with dual chambers composed of polymethyl methacrylate (PMMA). Each slide is 75 x 25 x 1.8 mm (W x D x H) with a chamber depth of 100 µm.

Supporting Documentation

TC20 Automated Cell Counter Quick Guide
TC20 Automated Cell Counter Instruction Manual