L-Lactate Assay Kit (Colorimetric/Fluorometric) ab65330 from Abcam

Product Specs
ItemL-Lactate Assay Kit (Colorimetric/Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65330
Quantity100 Test, 2000 Test
MethodFuncS
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Assay Time0h 40m
SensitivityMore than 0.001 mM
Detection Range0.001 - 10 mM
Research AreaMetabolism

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Website: http://www.abcam.com

(1) An siRNA targeting S6k1 identifies photoreceptor phospholipid metabolism as a contributor to lipid buildup in age-related macular degenerationMolecular Therapy Nucleic AcidsJune 16, 2026Shun-Yun Cheng, Delaney Giguere, San Kim, Johanna M. Seddon, Jillian Caiazzi, Katherine Gross, Nicholas McHugh, Dimas Echeverria, Julia F. Alterman, Heather Gray-Edwards, et al.same batch of the FACS experiment. Lactate assay A lactate assay (L-Lactate Assay kit, Abcam, Cat. # ab65330) was performed using six biological samples from 2-month-old mice, each composed of one retina from

(2) Hypertension Drives Protein Lactylation and Vascular Dysfunction in Skeletal MuscleHypertensionApril 22, 2026Milene T. Fontes, Paul Townsend, Landon Butler, Juliana M. Parente, Fênix A. Araujo, Tiago J. Costa, Gisele F. Bomfim, Laena Pernomian, Wenbin Tan, Brandon N. VanderVeen, et al.lactate concentration was measured using a quantitative lactate assay kit (Abcam; #ab65331 for muscle; #ab65330 for plasma). Muscle samples were deproteinized using the Deproteinizing Sample Preparation Kit-TCA (ab204708,

(3) ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinomaCell DiscovApril 21, 2026Lijie Han, Lina Gan, Balázs Schäfer, Dillon M. Voss, Mathias Danielsen, Ondrej Kostov, Jia Liu, Ting Wang, Marvin H. Caruthers, Hao Chen, et al.activity kit (ab83432; Abcam), and lactic acid production was measured with a L-Lactate Assay Kit (ab65330 and ab65331; Abcam) in accordance with the manufacturer’s instructions. Cells were either transfected

(4) Lactate Promotes Endothelial-Mesenchymal Transition via Mediating Twist1 Lactylation in Hypoxic Pulmonary HypertensionInternational Journal of Molecular SciencesFebruary 27, 2026Xingbing Li, Fengxian Wang, Ningxin Liu, Yu Liu, Weimin Yu, Ming Tanghypoxia-induced PH models. Lactate levels in plasma, or lung tissues were measured using a Lactate Assay Kit (ab65330, Abcam, Cambridge, UK). Detailed methodological descriptions are available in our prior publications

(5) Microglial serine racemase knockout alleviates Alzheimer-like neuropathology and behavioral deficit via lactylation-mediated anti-inflammationCommunications BiologyFebruary 25, 2026Jing Zhou, Yuanhong Yang, Shuyi Liu, Juan Chen, Hongji Liao, Wenjing Liang, Zhiwen Zhang, Yan Wang, Yimei Liu, He Zhang, et al.intracellular lactate Intracellular lactate levels were quantified using a L-lactate assay kit (Abcam, UK, cat#AB65330). Prior to measurement, the culture medium was discarded, and the cultured microglia were gently rinsed

(6) Ultrasmall episymbiont Nanosynbacter lyticus employs multiple ATP-generating metabolic pathways during horizontal transmissionThe ISME JournalJanuary 14, 2026Nusrat Nahar, Pu-Ting Dong, Jing Tian, Alex S Grossman, Erik L Hendrickson, Kristopher A Kerns, Mary Ellen Davey, Batbileg Bor, Jeffrey S McLean, Xuesong HeColorimetric/Fluorometric Assay Kit (Abcam, Cat# ab83355/K354), and L-Lactate Assay Kit (Abcam, Cat# ab65330), respectively, following each manufacturer’s instructions to evaluate TM7x metabolic activity. TM7x

(7) Lipoic Acid Ameliorates Lipopolysaccharide‐Induced Inflammation via Inhibition of Glycolysis in RAW264.7 MacrophagesImmunity, Inflammation and DiseaseJanuary 8, 2026Liduo Yue, Kai Wang, Rongyuan Wang, Linbei Lu, Lihong Faninterleukin (IL)‐6 (Cat. No. ab222503), and interleukin (IL)‐18 (Cat. No. ab216165), and lactate (Cat. No. ab65330) were purchased from Abcam Plc (UK). 2.2 Cell Culturing and LPS Treatments RAW246.7 cell line (Cat.

(8) Spatial heterogeneity of lactylation: insights into gene expression, metabolism, and lactate transport in human embryonic stem cellsBiology OpenJanuary 1, 2026Alexandra M. Kozlov, Zuleika C. L. Leung, Rachel B. Wilson, Sukhdeep Bhangal, Karen Nygard, Andrew M. Powell, Nica M. Borradaile, Dean H. Betts, Robert C. Cummings, briefly vented, and then incubated on ice for 5 min before storage at −80°C. The lactate assay (#ab65330; Abcam) was performed according to the manufacturer's colorimetric assay instructions. The following

(9) Propionibacterium acnes evades microbicidal phagocytosis by inhibiting the mitochondrial biogenesis of nucleus pulposus cellsThe FEBS JournalJanuary 1, 2026Lemeng Ren, Changwei Li, Juntao Sun, Yuehuan Zheng, Yucheng Jiao, Jiancheng Zheng, Fangke Zhang, Yazhou Lin, Wenjian Wu, Peng Caoconcentration of lactate was assessed using the lactate colorimetric/fluorometric assay kit (cat. no. ab65330, Abcam), with the assay conducted following the manufacturer's instructions. The standard curve was

(10) Evolutionary adaptation of anaerobic and aerobic metabolism to high sulfide and hypoxic hydrothermal vent crab, Xenograpsus testudinatusScientific ReportsDecember 15, 2025Chi Chen, Guan-Chung Wu, Yung-Che Tseng, Sylvie Dufour, Ching-Fong Chang5 min to remove particles. Lactate and glucose levels were detected using the L-Lactate Assay Kit (ab65330, Abcam, Cambridge, MA, USA) and Glucose Assay Kit (MAK263, Sigma-Aldrich) according to the manufacturer’s

(11) Lactate as a metabolic–epigenetic signal linking high-intensity interval training (HIIT) to miRNA-Centered remodeling of the skeletal muscle methylome and transcriptomeRedox BiologyDecember 1, 2025Lei Zhou, Soroosh Mozaffaritabar, Kumpei Tanisawa, Takuji Kawamura, Mitsuru Higuchi, Istvan Boldogh, Xueqing Ba, Sataro Goto, George Brooks, Yaodong Gu, et al.pyruvate concentrations in gastrocnemius muscle were measured using assay kits (Abcam; lactate kit ab65330, pyruvate kit ab65342, αKG kit ab83431, NAD/NADH kit ab176723). Approximately 20 ± 2 mg of homogenized

(12) Lactate‐mediated activation of GPR81 regulates BCR /Abl protein expression in chronic myeloid leukemia cells selected under low oxygen tensionThe Journal of PathologyDecember 1, 2025Giulio Menegazzi, Dayana Desideri, Alessio Biagioni, Elisabetta Rovida, Persio Dello Sbarba, Silvia Peppicellimanufacturer's protocol. L‐lactate concentrations were measured using the lactate assay kit (Catalogue No.: ab65330; Abcam), following the manufacturer's protocol, and using a 550 spectrophotometric microplate reader

(13) H3K18 lactylation in cancer-associated fibroblasts drives malignant pleural effusion progression via TNFR2+ Treg recruitmentExperimental & Molecular MedicineNovember 28, 2025Linlin Ye, Xuan Xiang, Zihao Wang, Siyu Zhang, Qianqian Xue, Xiaoshan Wei, Yao Liu, Haolei Wang, Jiaqi Ai, Bohan Yang, et al.levels Lactate concentrations were quantified using the Lactate Colorimetric/Fluorometric Assay Kit (#ab65330, Abcam). The enzyme working solution and chromogenic agent were added to the samples. Following adding

(14) SKA3-mediated hypoxia tolerance and metabolic reprogramming promote liver metastasis in lung adenocarcinomaCell Death & DiseaseNovember 26, 2025Zedong Sun, Minmin Zhou, Ningdan Song, Can Wang, Mingrui Liu, Simian He, Feifan Ji, Jiayu Chen, Lin Xu, Xiufen Zheng, et al.lactate secretion, and pyruvate production was performed using kits from Abcam (catalog nos. ab136955, ab65330, and ab65342) according to the manufacturer’s instructions. Cell counting was performed using a cell

(15) Hyperosmolarity-Induced Oxidative Stress Leads to Senescence in Human Corneal Epithelial Cells (HCEPC) via DNA Damage, Metabolic Disturbance and Mitophagy DeclineAntioxidants (Basel)November 19, 2025Yongjie Zhang, Tingjun Fanexperiment was conducted according to previous methods [27] and lactate was detected using an Assay Kit (ab65330, Abcam, Cambridge, UK). 2.13. Real-Time Reverse Transcription Polymerase Chain Reaction (RT-qPCR) Total

(16) Sleep is a therapeutic window for photostimulation of drainage of aging brainFrontiers of OptoelectronicsNovember 19, 2025Terskov Andrey, Adushkina Viktoria, Shirokov Alexander, Navolokin Nikita, Blokhina Inna, Zlatogorskaya Daria, Semiachkina-Glushkovskaia Anastasiia, Konstancia Sonina, Evsyukova Arina, Elizarova Inna, et al.method according to the standard Abcam protocol with the L-Lactate Colorimetric/Fluorometric Assay Kit (ab65330, Abcam, UK). The reaction mixture contained the 46 µL Lactate Assay Buffer, the 2 µL Lactate Probe,

(17) Tankyrases modulate the hypoxia response through non-catalytic mechanisms affecting HIF-1αCell Communication and SignalingNovember 5, 2025Esteban Zamudio-Martínez, Daniel Delgado-Bellido, Jerónimo Borrego-Pérez, Angel Garcia-Diaz, Ana Belén Herrera-Campos, José Manuel Rodríguez-Vargas, F. Javier Olivercollected, and the lactate concentration was measured via a colorimetric/fluorometric L-lactate assay kit (ab65330, Abcam, Cambridge, UK) following the manufacturer’s instructions with a VersaMax Microplate Reader (Molecular Devices

(18) Hypothalamic Astrocytes Exhibit Glycolytic Features Making Them Prone for Glucose SensingGliaNovember 1, 2025Geller S, Zanou N, Lagarrigue S, Zehnder T, Gouelle C, Santoro T, Repond C, Bezzi P, Amati F, Bouzier-Sore AK, et al.Switzerland). Lactate release into the medium was measured enzymatically using the L‐Lactate Assay Kit (Abcam ab65330) or, for the glucose concentration variations study, by the method of Rosenberg and Rush (1966). Both

(19) Anti-cancer efficacy of novel lonidamine derivatives: Design, synthesis, in vitro, in vivo, and computational studies targeting hexokinase-2European Journal of Medicinal ChemistryOctober 15, 2025Özgecan ŞAVLUĞ Şavluğ İpek, Bilgesu Onur Sucu, Saba Selvi, Fulya KOKSALAR Koksalar Alkan, Büşra Tiryaki, Hilmi Kaan Alkan, Ehsan Sayyah, İlayda Tolu, Mustafa Güzel, Serdar Durdağı, et al.Inc., Milpitas, CA, USA, #K607-100; currently named L-Lactate Assay Kit (Colorimetric/Fluorometric, #ab65330, Abcam, Cambridge, UK) was used. The collected cell medium was transferred into a new 96-well plate

(20) Histone lactylation promotes rheumatoid arthritis progression by increasing NFATc2 expression and the production of anti-lactylated histone autoantibodiesNat CommunOctober 10, 2025Gan Wu, Chenglin Yang, Yuan Huang, Bin Liu, Haige Zhai, Zelin Cao, Siyue Lu, Jijian Ji, Xinyue Yin, Xianming Xin, et al.measurement The concentration of lactate in synovial fluid was detected using L-Lactate Assay Kit (ab65330, Abcam, USA) according to the manufacturer’s instructions. The quantification was performed with Varioskan LUX multimode microplate reader

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L-Lactate Assay Kit (Colorimetric/Fluorometric) from Abcam

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L-Lactate Assay Kit (Colorimetric/Fluorometric) from Abcam

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