ROS-ID® Total ROS/Superoxide detection kit ENZ-51010 from Enzo Life Sciences, Inc.

Product Specs
ItemROS-ID® Total ROS/Superoxide detection kit
CompanyEnzo Life Sciences, Inc.
Price Supplier Page View Company Product Page
Catalog NumberENZ-51010
Quantity1 Kit
ApplicationsFlow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
Detection TargetROS

Specifications/Features

Directly Monitor Global Levels of Reactive Oxygen Species (ROS), and Specifically Superoxide

Distinguishes between different reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals
High sensitivity, specificity and accuracy for live cell studies
Compatible with major components of tissue culture media (phenol red, FBS and BSA)
Complete set of reagents, including ROS inducers and scavengers

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Enzo Life Sciences, Inc.

10 Executive Blvd.

Farmingdale, NY 11735

United States

Phone: 800-942-0430
Fax: 610-941-9252

Company Profile

Website: http://www.enzolifesciences.com

(1) Myo-Inositol modulates AKT signalling, mitochondrial protein expression and intracellular Ca²⁺dynamics in human dermal fibroblastsScientific ReportsJanuary 28, 2026Paola Zanfardino, Alessandro Amati, Domenico Iacobellis, Matilde Colella, Vittoria PetruzzellaSuperoxide) Intracellular ROS levels were measured using the ROS-ID® Total ROS/Superoxide Detection Kit (ENZ-51010, Enzo Life Sciences, NY, USA) following the manufacturer’s instructions. Equal numbers of fibroblasts

(2) Stem cells ameliorate neurotrauma-induced visual disturbances and retinal degeneration via broad normalization of β-catenin-related signalingInternational Journal of Medical SciencesJanuary 23, 2026Shu-Chun Kuo, Chung-Hsin Tseng, Suan Hwang, Chia-Yi Lee, Ting-Feng Wu, Pi-Yu Chao, Anthony Lu, Ching-Ping Chang, Chung-Ching Chiokit,,BD Biosciences,551302,Flow cytometry ROS-ID® Total ROS/Superoxide detection kit,,ENZO Life Sciences,ENZ-51010,Flow cytometry TUNEL Apoptosis Assay Kit,,BioTnA Biotech,TAAP01F-100,IF Luxol Fast Blue Stain Kit (Myelin Stain

(3) Modified Sanjia Powder ameliorates cognitive impairment and exerts neuroprotective effects in 5 × FAD mice: insights from quantitative proteomicsFrontiers in NutritionJanuary 16, 2026Yifan Wang, Chenxi Liu, Xiaoyan Zheng, Zhiqiang Wang, Yufan Wang, Yi Geng, Jin Yang, Kaifeng Wei, Xiaoying Chenwas measured using the ROS-ID® Total ROS/Superoxide Detection Kit (Enzo Life Sciences, catalog no. ENZ-51010) according to the manufacturer's instructions. Fluorescence was detected with a TECAN Infinite F200

(4) Exposure of cells to near-infrared irradiation relaxes chromatin compaction and facilitates recognition of cyclo-butane pyrimidine dimersScientific reportsJuly 1, 2025Plitta-Michalak B, Kolobynina KG, Qin Q, Jain I, Chen IP, Henning S, Volkmer B, Greinert R, Tham A, Boukamp P, et al.quantification ROS and superoxide were simultaneously, detected using the ROS-ID kit (Enzo Life Science ENZ-51010) according to the manufacturer’s recommendations. Total ROS were detected with Oxidative Stress Detection

(5) Mitochondria regulate inositol triphosphate-mediated Ca2+ release triggered by voltage-dependent Ca2+ entry in resistance arteriesJ PhysiolMay 5, 2025Xun Zhang, Charlotte Buckley, Matthew D. Lee, Susan Chalmers, Calum Wilson, John G. McCarronSigma‐Aldrich (Dorset, UK). The ROS‐ID Total ROS/Superoxide Detection Kit was obtained from Enzo Life Sciences (ENZ‐51010; Exeter, UK). Sylgard 184 silicone elastomer kit was obtained from Dow (Barry, UK). Several pharmacological

(6) Isorhamnetin Alleviates Renal Fibrosis by Inducing Endogenous Hydrogen Sulfide and Regulating Thiol-Based Redox State in Obstructed KidneysBiomoleculesSeptember 29, 2024Zhen Zhang, Haiyan Zhang, Jianyu Shi, Zheng Wang, Yanni Liang, Jingao Yu, Hongbo Wang, Zhongxing Song, Zhishu Tang, Dongbo Zhang, et al.superoxide anion (O2−) was detected by a ROS/Superoxide detection kit from Enzo (Lausen, Switzerland, ENZ-51010) following the manufacturer’s instructions [16]. The ROS detection dye (green probe) reacts directly

(7) Increased Expression of Transferrin Receptor 1 in the Brain Cortex of 5xFAD Mouse Model of Alzheimer’s Disease Is Associated with Activation of HIF-1 Signaling PathwayMolecular NeurobiologySeptember 1, 2024Sabrina Petralla, Liudmila Saveleva, Katja M. Kanninen, Julia S. Oster, Maria Panayotova, Gert Fricker, Elena Purisfinal concentration of 0.2 µg total protein/µL using the ROS-ID® Total ROS/Superoxide Detection Kit (ENZ-51010, Enzo Life Sciences, Farmingdale, NY, USA). All samples were incubated with the ROS/Superoxide Detection

(8) Gut bacteria exacerbates TNBS-induced colitis and kidney injury through oxidative stressRedox biologyJune 1, 2024Sui Y, Jiang R, Niimi M, Wang X, Xu Y, Zhang Y, Shi Z, Suda M, Mao Z, Fan J, et al.production Superoxide anion and reactive oxygen species (ROS) levels were measured using a commercial kit (Cat #ENZ-51010, Enzo Life Sciences, Farmingdale, NY, USA) per the manufacturer’s protocol. Cells were seeded in 96-well plates

(9) Oleuropein enhances proteasomal activity and reduces mutant huntingtin-induced cytotoxicityFrontiers in pharmacologyJanuary 1, 2024Huang ZN, Lee SY, Chen JM, Huang ZT, Her LS.Flow cytometry, the BD Biosciences FACS Caliber system (San Jose, CA), with Total ROS/SO detection kit (Cat#ENZ-51010, Enzo, United States of America) as suggested by the manufacturer’s instructions. Briefly, cells were

(10) Hyperbaric oxygen therapy suppresses hypoxia and reoxygenation injury to retinal pigment epithelial cells through activating peroxisome proliferator activator receptor‐alpha signallingJournal of Cellular and Molecular MedicineOctober 1, 2023Tzong‐Bor Sun, Kuo‐Feng Huang, Ko‐Chi Niu, Cheng‐Hsien Lin, Wen‐Pin Liu, Chao‐Hung Yeh, Shu‐Chun Kuo, Ching‐Ping Chang,BD Biosciences,–,551302,Flow cytometry ROS‐ID® Total ROS/Superoxide detection kit,ENZO Life Sciences,–,ENZ‐51010,Flow cytometry Superoxide Dismutase activity assay kit,Abcam,–,ab65354,Colorimetric analysis Catalase

(11) Conditional Knockout of Hypoxia-Inducible Factor 1-Alpha in Tumor-Infiltrating Neutrophils Protects against Pancreatic Ductal AdenocarcinomaInternational journal of molecular sciencesJanuary 1, 2023Sieow JL, Penny HL, Gun SY, Tan LQ, Duan K, Yeong JPS, Pang A, Lim D, Toh HC, Lim TKH, et al.Oxidative Stress Detection Reagent from the Total Reactive Oxygen Species Detection Kit (Enzo LifeSciences, ENZ-51010, Farmingdale, NY, USA) according to the manufacturer’s protocol. Neutrophils were incubated with the

(12) Increased Expression and Activity of Brain Cortical cPLA2 Due to Chronic Lipopolysaccharide Administration in Mouse Model of Familial Alzheimer’s DiseasePharmaceuticsNovember 10, 2022Mikko Gynther, Mariana Leal Estrada, Sanna Loppi, Paula Korhonen, Katja M. Kanninen, Tarja Malm, Jari Koistinaho, Seppo Auriola, Gert Fricker, Elena Purisprotein/μL. The production of total ROS was measured by the ROS-ID® Total ROS/Superoxide detection kit (ENZ-51010, Enzo Life Sciences, Farmingdale, NY, USA) according to the manufacturer’s instructions. Fluorescence

(13) Neutrophil degranulation and severely impaired extracellular trap formation at the basis of susceptibility to infections of hemodialysis patientsBMC medicineOctober 26, 2022Talal S, Mona K, Karem A, Yaniv L, Reut HM, Ariel S, Moran AK, Harel E, Campisi-Pinto S, Mahmoud AA, et al.intracellular ROS/RNS production in neutrophils, we used ROS-ID total ROS/Superoxide detection kit (ENZ-51010, ENZO Life Sciences). Neutrophils were isolated from blood samples taken from HC subjects and HD patients.

(14) Aberrant iron distribution via hepatocyte-stellate cell axis drives liver lipogenesis and fibrosisCell metabolismAugust 2, 2022Gao H, Jin Z, Bandyopadhyay G, Wang G, Zhang D, Rocha KCE, Liu X, Zhao H, Kisseleva T, Brenner DA, et al.oxygen species (ROS) were measured using ROS-ID Total ROS/Superoxide Detection Kit (Enzo Life Sciences, ENZ-51010). Cells were seeded at 500,000 cells per well in 50 μL of resuspension medium into clear 96-well plates

(15) A Soft Zwitterionic Hydrogel as Potential Coating on a Polyimide Surface to Reduce Foreign Body Reaction to Intraneural ElectrodesMoleculesMay 13, 2022Manuele Gori, Sara Maria Giannitelli, Gianluca Vadalà, Rocco Papalia, Loredana Zollo, Massimo Sanchez, Marcella Trombetta, Alberto Rainer, Giovanni Di Pino, Vincenzo Denaropurchased from ImmunoTools GmbH (Friesoythe, Germany); ROS-ID Total ROS/RNS/Superoxide Detection kit (ENZ-51010) was purchased from ENZO Life Sciences, Inc. (Farmingdale, New York, NY, USA); Everolimus (RAD001) was

(16) Inhibition of mitoNEET attenuates LPS-induced inflammation and oxidative stressCell death & diseaseFebruary 8, 2022Lee S, Seok BG, Lee SJ, Chung SW.detected using ROS-ID® total ROS/Superoxide Detection kit (Enzo Life Sciences, Farmingdale, NY, USA, ENZ-51010) according to the protocol of the manufacturer. Measurements were performed on a FACS Calibur (Becton

(17) Dysregulation of Caveolin-1 Phosphorylation and Nuclear Translocation Is Associated with Senescence OnsetCellsOctober 28, 2021Goutas A, Outskouni Z, Papathanasiou I, Satra M, Koliakos G, Trachana V.detected by staining with two fluorescent dyes from the ROS-ID® Total ROS/Superoxide detection kit (ENZ-51010, Enzo, Farmingdale, NY, USA). The intensity of the green dye (total ROS detection reagent) represents

(18) Tanshinone IIA Stimulates Cystathionine γ-Lyase Expression and Protects Endothelial Cells from Oxidative InjuryAntioxidants (Basel)June 23, 2021Qiaojing Yan, Zhimin Mao, Jingru Hong, Kun Gao, Manabu Niimi, Takahiko Mitsui, Jian Yaoof superoxide anion (O2•−) and ROS were detected using a kit from Enzo life sciences (Tokyo, Japan, ENZ-51010) following the protocol provided by the manufacturer [32,33,35]. Briefly, cells in 96-well plates were

(19) Rutin Modulates MAPK Pathway Differently from Quercetin in Angiotensin II-Induced H9c2 Cardiomyocyte HypertrophyInternational journal of molecular sciencesMay 11, 2021Siti HN, Jalil J, Asmadi AY, Kamisah Y.superoxide in living cells was done using a commercial kit (ROS-ID® Total ROS/Superoxide Detection Kit, ENZ-51010, Enzo Life Science Ltd., NY, USA). The cardiomyocytes were seeded at cell density 1 × 104 cells/ well

(20) Reductively modified albumin attenuates DSS-Induced mouse colitis through rebalancing systemic redox stateRedox BiologyMay 1, 2021Xiawen Yang, Zhimin Mao, Yanru Huang, Haizhao Yan, Qiaojing Yan, Jingru Hong, Jianglin Fan, Jian Yao(O2•－) and ROS was detected by using a commercially available kit from Enzo life sciences (Tokyo, Japan, ENZ-51010) following the manual of the manufacturer, as previously described in our previous reports [35,37].

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ROS-ID® Total ROS/Superoxide detection kit from Enzo Life Sciences, Inc.

Description

Specifications/Features

Enzo Life Sciences, Inc.

Citations (47)

Reviews

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ROS-ID® Total ROS/Superoxide detection kit from Enzo Life Sciences, Inc.

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