CYTO-ID® Autophagy detection kit ENZ-51031 from Enzo Life Sciences, Inc.

Product Specs
ItemCYTO-ID® Autophagy detection kit
CompanyEnzo Life Sciences, Inc.
Price Supplier Page View Company Product Page
Catalog NumberENZ-51031
Quantity50 tests, 200 tests
ApplicationsFlow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

Specifications/Features

Kit/Set Contains: Cyto-ID^® Green Detection Reagent, 25 µl
Hoechst 33342 Nuclear Stain, 50 µl
Autophagy Inducer (Rapamycin, 12.5 nmol)
10X Assay Buffer, 30 ml

Features & Benefits:

No transfection required
Proprietary dye includes titratable moieties specific for selectively staining autophagic vesicles
Protocol validated with known inhibitors and activators of autophagic activity
Rapidly quantifies autophagy in native heterogeneous cell populations
Eliminates need for time and effort-consuming transfection efficiency validation required with LC3-GFP transfection
Selective and comprehensive staining, allows measurement and differentiation between autophagic flux and autophagolysosome accumulation
Negligible staining of lysosomes reduces background seen with other dyes
Facilitates high-throughput screening of activators and inhibitors of autophagy

Mainfeature: A No-transfection, Quantitative Assay for Monitoring Autophagy in Live Cells

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Enzo Life Sciences, Inc.

10 Executive Blvd.

Farmingdale, NY 11735

United States

Phone: 800-942-0430
Fax: 610-941-9252

Company Profile

Website: http://www.enzolifesciences.com

(1) Arginine deprivation induces prolonged autophagy activation and ROS-dependent cell death in glioblastoma multiformeCNS OncolMay 7, 2026Maya Abdelkhalek, Yasmin El-Jawhari, Maha Shaheen, Rawan Saad, Majd Sarieddine, Nour El-Husseini, Mirvat El-Sibai, Ralph J. Abi-Habibto the company’s manual (ENZO product manual - CYTO-ID® Autophagy Detection Kit, 2016 - Catalog No. ENZ-51031 - Part D. Live Cell Analysis by Flow Cytometry). Results were read on a C6 flow cytometer (BD Accuri,

(2) Stanniocalcin 2-Induced Autophagy Confers Resistance of Lung Cancer Cells to EGFR Inhibition via the ERK/Beclin 1 SignalingCancer MedApril 27, 2026Yi-Nan Liu, Yi-Ling Chen, Meng-Feng Tsai, Shang-Gin Wu, Tzu-Hua Chang, Tzu-Hsiu Tsai, Jin-Yuan Shihunder a Leica DM4000B fluorescent microscope. For the detection of autophagic vacuoles, a Cyto‐ID kit (#ENZ‐51031, Enzo Life Sciences, Farmingdale, New York, USA) was used, and the results were visualized using a FACSCalibur

(3) Integrative genomic and functional analyses reveal NINL as a modulator of tau aggregationAlzheimers DementApril 9, 2026Samantha K. Swift, Guangming Huang, J. Nicholas Cochran, Ricardo D'Oliveira Albanus, Miguel A. Minaya, Patricia A. Castruita, Rui Zhang, Katherine J. Miller, Emma Starr, Grant Galasso, et al.and number were measured by confocal microscopy using CytoID Autophagy detection kit (Enzo, Cat #: ENZ‐51031). 37 HEK293T cells were transiently transfected with control or NINL plasmids. Twenty‐four hours after

(4) Active Colitis Attenuates Ventricular Excitation–Contraction Coupling by T-Tubular RemodelingBiomoleculesMarch 27, 2026Edward J. Ouille V, Carlos H. Pereira, Ygor Marinho, Giedrius Kanaporis, Kathrin BanachCYTO-ID®, a dye that selectively accumulates in autophagic vacuoles and Hoechst33342 for nuclear staining (ENZ-51031, Enzo Life Science, Farmingdale, NY, USA). Using confocal microscopy (Nikon A1R, Nikon Instruments Inc.,

(5) Integrated Functional and scRNA-Seq Analyses Reveal Convergence of M-CSF- and GM-CSF-Derived Macrophages Following IL-27 PolarizationCellsMarch 16, 2026Tomozumi Imamichi, Jun Yang, Qian Chen, Udeshika Kariyawasam, Mayra Marquez, Jeanette Higgins, Jordan Metz, Homa Nath Sharma, Michael W. Baseler, Hongyan Sui1000 cells. 2.6. Autophagy Assay Autophagy was assessed using the Cyto-ID Autophagy Detection Kit (ENZ-51031; Enzo Life Sciences, Farmingdale, NY, USA) with modifications to a previous report [28]. Polarized cells

(6) Alkyltriphenylphosphonium Binding to Cardiolipin Triggers Oncosis in Cancer CellsAdvanced ScienceMarch 1, 2026Jin Li, Hang Zheng, Yuxing Lin, Ziwei Zhao, Yijia Mai, Zhangrong Lou, Qiang Liu, Zhao Ma, Chengjun Wuwere washed twice with the assay buffer and stained with 100 µL of dual detection reagent (Enzo Life, #ENZ‐51031) for 30 min at 37 °C in the dark. Cells were then gently washed with assay buffer and were observed

(7) Synthesis and cytotoxicity of novel cyanochalcones: induction of cell cycle arrest, apoptosis, and autophagy in HEP2 and MCF-7 cellsNaunyn-Schmiedeberg's Archives of PharmacologyJanuary 15, 2026Nada S. Ibrahim, Amr Ahmed WalyEldeen, Sherif Abdelaziz Ibrahim, Ismail A. Abdelhamid, Heba K. A. ElhakimGreen detection reagent (fluorescent marker; CYTO-ID® Autophagy Detection Kit, Enzo Life Sciences, ENZ-51031), a cationic amphiphilic dye that selectively labels autophagic vesicles (pre-autophagosomes, autophagosomes,

(8) Integrative Genomic and Functional Analyses Reveal NINL as a Modulator of Tau AggregationbioRxiv : the preprint server for biologyDecember 16, 2025Swift SK, Huang G, Cochran JN, Minaya MA, Castruita PA, Miller KJ, Starr E, Galasso G, Marsh JA, Kao AW, et al.and number were measured by confocal microscopy using CytoID Autophagy detection kit (Enzo, Cat #: ENZ-51031)[33]. Twenty-four hours after transfection, cells were replated at a density of 200,000 cells per well

(9) Dual mechanism of autophagy gene repression by PHF23 and therapeutic potential of its inhibition in protein aggregation disordersNucleic Acids ResDecember 8, 2025Seon Ah Choi, Jaebeom Kim, Young Suk Yu, Jaemin Kim, Se In Kim, Hamin Bae, Junhee Kwon, Keun Il Kim, Sung Hee Baekstaining Autophagic vacuoles were stained by fluorescence microscopy using the CYTO-ID autophagy detection kit (ENZ-51031, Enzo Life Sciences). Cells grown on coverslips at a density of 5 × 104 cells were incubated with a

(10) In Vitro Study Regarding Cytotoxic and Inflammatory Response of Gingival Fibroblasts to a 3D-Printed Resin for Denture BasesJournal of Functional BiomaterialsNovember 27, 2025Miruna Dinescu, Lucian Toma Ciocan, Ana Maria Cristina Țâncu, Vlad Gabriel Vasilescu, Bianca Voicu-Balasea, Florentina Rus, Alexandra Ripszky, Silviu-Mirel Pițuru, Marina ImreFluorescence Staining Assays For autophagy assessment, the CYTO-ID® Autophagy Detection Kit (catalog number: ENZ-51031, Enzo Life Sciences, Inc., Farmingdale, NY, USA) was used after exposing fibroblasts to dental material

(11) Modulation of cell viability, apoptosis, and autophagy in pancreatic cancer cells using therapeutic ultrasound with microbubbles and gemcitabineUltrasonographyNovember 1, 2025Seunghyeon Roh, Jae Young Lee, Eun-Joo Park, Yuri Cheon, Yun Deok Ahnactivation in treated cells was assessed using the Sony SA3800 FACS system and an autophagy detection kit (ENZ-51031, CYTO-ID autophagy detection kit, Enzo Life Sciences). Cells were harvested and washed with PBS. After

(12) SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesisNat CommunOctober 3, 2025Kaushal Asrani, Adrianna Amaral, Juhyung Woo, Sanaz Nourmohammadi Abadchi, Thiago Vidotto, Eddie Imada, Alyza Skaist, Kewen Feng, Hans B. Liu, Mithila Kasbe, et al.Cell Signaling), Cycloheximide (#2112, Cell Signaling), Magic Red Cathepsin B kit (#ICT937, BIO-RAD), CYTO-ID (#ENZ-51031, Enzo), Pierce anti-HA-magnetic beads (#88837, Thermo Fisher Scientific), Taqman Gene Expression Master

(13) Synthesis of CNP-SAR405 delivery system for improved therapeutics anticancer activity using A549 human lung cancer cell linesNaunyn-Schmiedeberg's Archives of PharmacologySeptember 25, 2025Ruqayyah A. Bashirah, Nur Balqis Zamri, Noorjahan Banu Alitheen, Saila Ismail, Mas Jaffri Masarudin1000 rpm for 5 min. Supernatant from each tube were removed and CYTO- ID® Autophagy Detection Kit (ENZ-51031) (Enzo Life Sciences) was added into each tube for 30 min incubation in the dark. All samples were analyzed

(14) Interconnections of insulin/IGF-1 signaling and autophagy abnormalities in Alzheimer's diseaseAlzheimers DementJuly 4, 2025Wang Liao, Jinfeng Xuan, Woo-In Ryu, Mariana K. Bormann, Yoon Lee, Haley Dion, Elizabeth Evangelista, Ruiyi Li, Lucas Trambaiolli, Jun Liu, et al.pharmacological induction of autophagy was measured with the CYTO‐ID Detection Kit (Enzo Life Sciences, ENZ‐51031‐0500). Cells were plated in duplicate at a density of 1.5 × 104 cells/well in a black wall, clear bottom

(15) Impact of environmental microplastic exposure on HepG2 cells: unraveling proliferation, mitochondrial dynamics and autophagy activationPart Fibre ToxicolJune 17, 2025Hana Najahi, Nicola Alessio, Massimo Venditti, Ida Lettiero, Domenico Aprile, Gea Oliveri Conti, Tiziana Cappello, Giovanni Di Bernardo, Umberto Galderisi, Sergio Minucci, et al.in living cells were conducted utilizing the Cyto-ID® Autophagy Detection Kit (Enzo Life Sciences, ENZ51031). This kit uses a cationic amphiphilic dye that selectively marks autophagic vacuoles. HepG2 were cultured

(16) LncRNA MIR155HG suppresses cell apoptosis by activating autophagy via miR-7036b-3p/GPNMB axis and AMPK/mTOR signaling in spinal cord injuryNon-coding RNA ResearchMay 22, 2025Ruoxi Liu, Jintao Ye, Sihua HuangStates). 2.4 CYTO-ID detection of autophagosome formation The Cyto-ID® autophagy detection kit (#ENZ-51031; Enzo Life Sciences, Farmingdale, NY, USA) was employed to estimate autophagy of primary spinal neurons.

(17) Effects of tianeptine on mTORC1-mediated neuronal autophagy in primary rat hippocampal neurons under nutrient deprivationSci RepApril 25, 2025Mi Kyoung Seo, Hyewon Kim, Ah Jeong Choi, Dae-Hyun Seog, Weon-Gyu Kho, Sung Woo Park, Jung Goo Leeincubation, cells were stained for autophagic vacuoles using the Cyto-ID® Autophagy Detection Kit (#ENZ-51031, Enzo, Farmingdale, NY, USA) according to the manufacturer’s protocol. In brief, the cells were washed

(18) Ancient genomic linkage of α-globin and Nprl3 couples metabolism with erythropoiesisNat CommunMarch 24, 2025Alexandra E. Preston, Joe N. Frost, Megan R. Teh, Mohsin Badat, Andrew E. Armitage, Ruggiero Norfo, Sarah K. Wideman, Muhammad Hanifi, Natasha White, Noémi BA. Roy, et al.triplicate. Chloroquine was added to one of the wells for each sample (50 μM; Enzo Life Sciences; Cat. No., ENZ-51031). The plate was incubated at 37 °C and 5% CO2 for 2 h. Cells were then processed using the CYTO-ID Autophagy Detection Kit

(19) Niemann-Pick C-like Endolysosomal Dysfunction in DHDDS Patient Cells, a Congenital Disorder of Glycosylation, Can Be Treated with MiglustatInt J Mol SciFebruary 10, 2025Hannah L. Best, Sophie R. Cook, Helen Waller-Evans, Emyr Lloyd-Evanswas determined using the fluorescent small molecule marker CYTO-ID from the CYTO-ID detection kit (ENZ-51031, ENZO life sciences, Exeter, UK) in live cells. The cells were incubated with CYTO-ID according to the

(20) Hypoxia‑induced SREBP1‑mediated lipogenesis and autophagy promote cell survival via fatty acid oxidation in breast cancer cellsOncol LettFebruary 7, 2025Jae-Ha Jung, Yeseul Yang, Yongbaek Kimand MDA-MB-231 cells were washed with assay buffer and subsequently incubated with Cyto-ID (cat. no. ENZ-51031; Enzo Life Sciences) at a 1:2,000 dilution in assay buffer for 30 min at 37°C in the dark. After washing

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CYTO-ID® Autophagy detection kit from Enzo Life Sciences, Inc.

Description

Specifications/Features

Enzo Life Sciences, Inc.

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CYTO-ID® Autophagy detection kit from Enzo Life Sciences, Inc.

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