Fig 1: Proteomic analysis of pretreatment plasmas from iKAP mice with complete tumor regression and with residual tumors. (A). Venn diagrams of pathways are significantly associated with more than a two-fold increase of proteins in the plasma of Regression mice or Non-regression mice compared to control mice. (B). Pathways uniquely identified in the comparison of Non-regression vs. control. p values were calculated by hypergeometric test. (C). Schema of mouse Vegfr3, Ctsb, and Igfbp4. Gray bars indicate peptides identified in mouse plasma. Numbers indicate mass spectra counts for each peptide. The amino acid sequences are based on P35917-1 for Vegfr3, P10605-1 for Ctsb, and P47879-1 for Igfbp4.
Fig 2: Validation of biomarker candidates in the pretreatment LARC set. (A). Levels of VEGFR3, CTSB, IGFBP4, CEA, EGFR, Ki-67, COX2, and E-cadherin in plasmas collected before nCRT from LARC patients who achieved pCR (pCR; n = 6) and LARC patients who had residual tumors after nCRT (non-pCR; n = 28). Horizontal lines indicate mean and standard deviation. p values were calculated using Welch’s t-test. (B). Receiver operating characteristic curves for VEGFR3, EGFR, COX2, and their combination in the pretreatment LARC set. CEA: Carcinoembryonic antigen, EGFR: Epidermal growth factor receptor, COX2: Prostaglandin G/H synthase 2, AUC: the area under the curve.
Fig 3: CTSB Skeletal Muscle Protein and Transcript Response to 80% Acute Aerobic Exercise. A) Representative western blot probing for CTSB before, 30-minutes post and 3-hours post 80% acute AE. All samples were normalized to total protein stain. Protein and transcript content of B) Mature CTSB protein, C) ProCTSB protein, D) Total CTSB protein and E) Pro:Mature CTSB protein ratio and F) CTSB transcript n = 15 (8M/7F). Mature CTSB is expected at 30 kD, ProCTSB is expected at 41 KD. All data are represented as mean±SEM. n = 13 PRE (7M/6F), n = 6 POST (5M/1F), and n = 6 3 h (4M/2F). *p < 0.05 vs PRE, **p < 0.01 vs PRE, #p < 0.05 main effect of time.
Fig 4: Effects of Acute Aerobic Exercise Intensity on Circulating CTSB. Circulating CTSB before and 30-minutes post AE A) 40% of VO2max, B) 65% of VO2max, C) 80% of VO2max and D) at VO2max. n = 15 (9M/6F) 80% VO2max, n = 16 (10M/6F) all other timepoints. All data are represented as mean±SEM. *p < 0.05 vs PRE.
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