Kinase-Glo Plus Luminescent Kinase Assay V3771 from Promega

Product Specs
ItemKinase-Glo Plus Luminescent Kinase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV3771
Detection Target3-phosphate dehydrogenase (GAPDH)
Detectionluminescence
Quantity10ml
MethodLuminescent

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Ancestral protein reconstruction reveals the mechanism of substrate specificity in FN3K-mediated deglycationbioRxivJuly 31, 2025Jenet K. Matlack, Robert E. Miner, Jameela Lokhandwala, Jennifer M. Binningdiluted kinase. The reaction was quenched after 30 minutes using the Kinase-Glo® Plus reagent (Promega #V3771). The final reaction was incubated for 10 minutes at room temperature. Luminescence was measured using

(2) PFKM phosphorylates histone H3 and promotes mitotic progression by sensing the levels of citrateNat CommunJuly 22, 2025Pianpian Lin, Yijun Qi, Huiying Chu, Hongyu Wu, Yajuan Zhang, Xiaolan Huang, Chen Li, Xiaoyan Xu, Hong Gao, Rong Zeng, et al.monitoring the luminescence signal that accompanies the remaining ATP using the Kinase-Glo assay (Promega, V3771). In vitro kinase assay For phosphorylation reactions, recombinant His-PFKM from E. coli or immunoprecipitated

(3) The accessory protein CvnF8 modulates histidine kinase activity in an Actinobacterial G protein system in Streptomyces coelicolorbioRxivJuly 4, 2025Luis M. Cantu Morin, Kilian Dekoninck, Kyung-Yoon Min, Matthew F. Traxlerturnover kinetics was measured in triplicate using the Kinase-Glo Plus Luminescence Assay (Promega #V3771) in 384-well white plates (Greiner bio-one #5678–1075) with a final reaction volume of 10 μL. Reactions

(4) Characterization of AB598, a CD39 Enzymatic Inhibitory Antibody for the Treatment of Solid TumorsMolecular cancer therapeuticsOctober 1, 2024Anderson AE, Parashar K, Jin K, Clor J, Stagnaro CE, Vani U, Singh J, Chen A, Guan Y, Talukdar P, et al.measurements CD39 enzymatic inhibition was measured by ATP depletion with Kinase Glo Plus Assay (Promega, Cat. No. V3771) or AMP formation with AMP Glo Assay (Promega, Cat. No. V5012). The assays were run in the presence

(5) Discovery of pulmonary fibrosis inhibitor targeting TGF-β RI in Polygonum cuspidatum by high resolution mass spectrometry with in silico strategyJournal of Pharmaceutical AnalysisDecember 1, 2022Huarong Xu, Jiameng Qu, Jian Wang, Kefei Han, Qing Li, Wenchuan Bi, Ran Liuobtained from ImmunoClone (Houston, TX, USA). Kinase-Glo Luminescent Kinase Assay (catalogue number V3771) was purchased from Promega (Madison, WI, USA). Distilled water was provided by Wahaha Company (Hangzhou,

(6) Inhibitors targeting the autophosphorylation of serine/threonine kinase of Streptococcus suis show potent antimicrobial activityFrontiers in MicrobiologySeptember 2, 2022Haotian Li, Tingting Li, Qiao Hu, Zhiming Yao, Lu Li, Qi Huang, Rui Zhoucompound or DMSO. The mixture was incubated at 37°C for 30 min. Then, 50 μl of Kinase Glo® reagent (Cat# V3771, Promega, Madison, United States) was added to each well. After 10 min, the relative light unit (RLU)

(7) Inhibition of the MAP2K7-JNK pathway with 5Z-7-oxozeaenol induces apoptosis in T-cell acute lymphoblastic leukemiaOncotargetAugust 31, 2021Chen TJ, Du W, Junco JJ, Bridges CS, Shen Y, Puppi M, Rabin KR, Lacorazza HD.enzymatic activity was measured through consumption of ATP (Kinase-GLO® Plus Kinase Assay System, Promega #V3771) or generation of ADP (ADP-GLO, Promega #V6930). Luminescence values were calculated as the percentage

(8) In Vitro Characterization of Guanylyl Cyclase BdPepR2 from Brachypodium distachyon Identified through a Motif-Based ApproachInternational Journal of Molecular SciencesJune 10, 2021Maria Duszyn, Brygida Świeżawska-Boniecka, Aloysius Wong, Krzysztof Jaworski, Adriana Szmidt-JaworskaActivity For quantification of BdPepR2 protein kinase activity, a Kinase-Glo plus luminescent kinase assay (V3771, Promega, Walldorf, Germany) was used according to the manufacturer’s protocol. Briefly, purified truncated

(9) Structural Basis for Helicase-Polymerase Coupling in the SARS-CoV-2 Replication-Transcription ComplexCellSeptember 1, 2020James Chen et al.Assays,Critical Commercial Assays,Critical Commercial Assays ,, Kinase-Glo Plus Assay Kit,Promega,Cat.# V3771 ,, Deposited Data,Deposited Data,Deposited Data ,, Coordinates of SARS-CoV-2 nsp132-RTC,This paper,PDB:

(10) Measurement of ATPase Activity of Valosin-containing Protein/p97BIO-PROTOCOLFebruary 5, 2020Kruthi Suvarna, Kaori Honda, Makoto Muroi, Yasumitsu Kondoh, Hiroyuki Osada, Nobumoto Watanabecatalog number: SML0031, CAS number: 177355-84-9) 31. Kinase Glo Plus reagent (Promega, catalog number: V3771, Reference 8) 32. Assay buffer (1x; see Recipes) 33. ATP solution (see Recipes) 34. Kinase-glo reagent

(11) Developing a novel dual PI3K–mTOR inhibitor from the prodrug of a metaboliteOncoTargets and TherapyNovember 10, 2017Yan Zhou, Genyan Zhang, Feng Wang, Jin Wang, Yanwei Ding, Xinyu Li, Chongtie Shi, Jiakui Li, Chengkon Shih, Song You(14-770), respectively. A Kinase-Glo Plus luminescent kinase-assay kit was purchased from Promega (V3771). U87MG, BT474, A549, SKOV-3, PC-3, HCT116, and 786-0 cell lines were purchased from ATCC. RPMI- 1640 medium

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Kinase-Glo Plus Luminescent Kinase Assay

Kinase-Glo Plus Luminescent Kinase Assay from Promega

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Kinase-Glo Plus Luminescent Kinase Assay from Promega

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