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Assay Kits
Assay Kit
First Strand cDNA Synthesis Kit
First Strand cDNA Synthesis Kit from OriGene Technologies
Be the first to write a review!
Citations:
(68)
Supplier Page
Supplier Page from
OriGene Technologies for
First Strand cDNA Synthesis Kit
Description
First Strand cDNA Synthesis Kit (11801-100)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
First Strand cDNA Synthesis Kit
Company
OriGene Technologies
Price
$540.00
Supplier Page
View Company Product Page
Catalog Number
NP100042
Type
cDNA Synthesis Kits
Applications
cDNA Synthesis
Quantity
100 reactions
Add to Compare List
OriGene Technologies
9620 Medical Center Drive # 200
Rockville
,
Maryland
20850
United States
Phone
: 1-888-267-4436 (U.S. only) / 301-340-3188
301-340-9254
Company Profile
Website:
http://www.origene.com
Citations (68)
(1)
Protocol for the generation and xenotransplantation of human induced pluripotent stem cell-derived neural progenitor cells into the mouse forebrain
STAR Protocols
March 1, 2026
Julie J. McInvale, Chloe E. Ayers, Hemanta Sarmah, Kang Kim, Peter Reinhardt, Aayushi Mahajan, Nelson Humala, Barbara Corneo, Jared Sterneckert, Peter Canoll,
et al.
MicroAmp Optical 96-Well Reaction Plate
,
Thermo Fisher Scientific
,Cat#
N8010560
First strand cDNA synthesis kit
,
Origene
,Cat#
NP100042
RNeasy Plus Mini kit,
QIAGEN
,Cat#
74134
PowerUp SYBR Green Master Mix
for
qPCR
,
Thermo Fisher Scientific
,Cat#
(2)
Pharmacologic Modulation of the PAR-2–ERK Axis by Statins Converts Inflammatory Survival Signalling into Apoptosis in Colorectal Cancer Cells
International Journal of Molecular Sciences
January 16, 2026
Layla Amiri, Rajashree Patnaik, Riah Lee Varghese, Bintul Huda, Yajnavalka Banerjee
samples were reverse-transcribed into complementary DNA (cDNA) using the
First-Strand cDNA Synthesis Kit
(
NP100042
,
OriGene
, Heidelberg, Germany), in strict accordance with the manufacturer’s protocol. The resulting
(3)
Placental‐Derived Connective Tissue Matrix Mediates Murine Recurrent Laryngeal Nerve Regeneration
The Laryngoscope
December 22, 2025
Sunjay Anekal, Ananya Tadikonda, Gabriel Sobczak, Lena W. Chen, Aditya Bhatt, Troy Wesson, Patrick R. Finnegan, Stacey Halum
acetylcholine receptor (nAchR) gene].
RT‐PCR
was performed using the
First Strand cDNA Synthesis kit
(Catalog#
NP100042
;
Origene Technologies
Inc.; Rockville, MD).
qPCR
was performed using Origene
Universal SYBR Green qPCR Master Mix
(4)
Gestational stress disrupts the neuroimmune environment in the nucleus accumbens of maternal rats
Brain, Behavior, & Immunity - Health
December 1, 2025
Courtney N. Dye, Dominic V. Franceschelli, Caitlin Goodpaster, Lynde M. Wangler, Amanda Ringland, Jonathan P. Godbout, Kathryn M. Lenz, Benedetta Leuner
RNeasy Mini Kit (QIAGEN [741014]). cDNA was synthesized using
First Strand cDNA synthesis kit
(
Origene
[
NP100042
]).
qPCR
was conducted for the following synaptic genes:
VGLUT1
, VGLUT2, synaptophysin (SYN),
PSD95
,
(5)
CDK12 regulates cellular metabolism to promote glioblastoma growth
JCI Insight
November 10, 2025
Jeong-Yeon Mun, Chang Shu, Qiuqiang Gao, Zhe Zhu, Hasan O. Akman, Mike-Andrew Westhoff, Georg Karpel-Massler, Markus D. Siegelin
extracted RNA was reverse transcribed into cDNA by employing the
cDNA synthesis kit
from
Origene
(catalog
NP100042
).
qPCR
was conducted with Power
SYBR Green PCR Master Mix
(
Applied Biosystems
,
4367659
), and the
amplification
(6)
Curcumin Inhibits Protease Activated Receptor 2-Induced ERK Phosphorylation Calcium Mobilization and Anti-Apoptotic Signaling in Inflammation-Driven Colorectal Cancer Cells
Cells
September 16, 2025
Rajashree Patnaik, Riah Varghese, Ahad Al-Kabani, Shirin Jannati, Yajnavalka Banerjee
were then reverse-transcribed into complementary DNA (cDNA) using the
First-Strand cDNA Synthesis Kit
(
NP100042
,
OriGene
, Heidelberg, Germany), strictly following the manufacturer’s protocol [106]. The resulting
(7)
D-chiro-inositol effectively counteracts endometriosis in a mouse model
Molecular medicine (Cambridge, Mass.)
April 11, 2025
Placidi M, Casoli G, Vergara T, Bianchi A, Cocciolone D, Zaccardi S, Macchiarelli G, Palmerini MG, Tatone C, Bevilacqua A,
et al
.
converted into complementary DNA (cDNA) using a reverse transcription kit (
OriGene Technologies
, cat.
NP100042
, Rockville, MD, USA). The cDNA was used for
RT-PCR
reactions, using the Applied Biosystems 7300 system
(8)
Peripartum buprenorphine and oxycodone exposure impair maternal behavior and increase neuroinflammation in new mother rats
Brain, Behavior, and Immunity
February 1, 2025
Courtney N. Dye, Aliyah I. Webb, Madison P. Fankhauser, Jordyn J. Singleton, Aravind Kalathil, Amanda Ringland, Benedetta Leuner, Kathryn M. Lenz
synthesized according to manufacturer’s instructions using a
First Strand cDNA synthesis kit
(
Origene
[
NP100042
]).
qPCR
was then conducted using Sso Advanced Universal SYBR Green Supermix (
Biorad
[
1725272
]) in a
(9)
Osteopontin drives neuroinflammation and cell loss in MAPT-N279K frontotemporal dementia patient neurons
Cell Stem Cell
May 2, 2024
Osama Al-Dalahmah, Matti Lam, Julie J. McInvale, Wenhui Qu, Trang Nguyen, Jeong-Yeon Mun, Sam Kwon, Nkechime Ifediora, Aayushi Mahajan, Nelson Humala,
et al.
Assays
CellTiter 96(R) AQueous One Solution
Assay
,
Promega
,Cat#
G3580
First strand cDNA synthesis kit
,
Origene
,Cat#
NP100042
CellRox DeepRed,
ThermoFisher
,Cat#
C10422
Fluorescent latex beads (
latex beads-rabbit IgG-FITC complex
),
Cayman chemical
(10)
Clinical and molecular features of acquired resistance to immunotherapy in non-small cell lung cancer
Cancer cell
February 12, 2024
Memon D, Schoenfeld AJ, Ye D, Fromm G, Rizvi H, Zhang X, Keddar MR, Mathew D, Yoo KJ, Qiu J,
et al
.
assays
,Critical commercial
assays
,Critical commercial
assays
First Strand cDNA Synthesis Kit
,
Origene
,
NP100042
RNeasy Kit,
Qiagen
,
75144
QIAshredder
,
Qiagen
,
79656
RNase-Free DNase Set,
Qiagen
,
79254
SsoAdvanced Universal SYBR Green Supermix
(11)
Acyl-Carnitines Exert Positive Effects on Mitochondrial Activity under Oxidative Stress in Mouse Oocytes: A Potential Mechanism Underlying Carnitine Efficacy on PCOS
Biomedicines
September 6, 2023
Martina Placidi, Teresa Vergara, Giovanni Casoli, Irene Flati, Daria Capece, Paolo Giovanni Artini, Ashraf Virmani, Samuele Zanatta, Anna Maria D’Alessandro, Carla Tatone,
et al.
Applied Biosystems
, Foster City, CA, USA). According to the protocol of the reverse transcription kit (
NP100042
,
OriGene Technologies
, Rockville, MD, USA), RNA (1 μg) was converted into complementary DNA (cDNA).
(12)
Ocular stress enhances contralateral transfer of lenadogene nolparvovec gene therapy through astrocyte networks
Molecular Therapy
July 1, 2023
Nolan R. McGrady, Andrew M. Boal, Michael L. Risner, Magali Taiel, Jose A. Sahel, David J. Calkins
(
Thermo Fisher Scientific
). RNA was first reverse transcribed with the
First Strand cDNA Synthesis Kit
(
Origene
, Cat#
NP100042
) using a SimpliAmp thermocycler (Thermo Fisher Scientific) with the following program; 1 cycle at 22°C
(13)
BAP1 maintains HIF-dependent interferon beta induction to suppress tumor growth in clear cell renal cell carcinoma
Cancer letters
October 28, 2022
Langbein LE, El Hajjar R, He S, Sementino E, Zhong Z, Jiang W, Leiby BE, Li L, Uzzo RG, Testa JR,
et al
.
transcription was performed with 0.5–2.5 μg RNA using the
First Strand cDNA Synthesis Kit
(
Origene
,
NP100042
, Rockville, MD, USA),
RevertAid First Strand cDNA Synthesis Kit
(
Thermo Fisher
,
K1622
), or
Invitrogen
(14)
Lactate is an epigenetic metabolite that drives survival in model systems of glioblastoma
Molecular cell
August 18, 2022
Torrini C, Nguyen TTT, Shu C, Mela A, Humala N, Mahajan A, Seeley EH, Zhang G, Westhoff MA, Karpel-Massler G,
et al
.
,
Thermo Fisher
,Cat#
C10632
miRNeasy Mini kit
,
Qiagen
,Cat#
217004
First Strand cDNA Synthesis Kit
,
Origene
,Cat#
NP100042
PerfeCta® SYBR® Green FastMix,VWR,Cat# 101414-270 SimpleChIP® Enzymatic
Chromatin
IP Kit,
Cell signaling
,Cat#
(15)
Kynurenine Monooxygenase Expression and Activity in Human Astrocytomas
Cells
August 8, 2021
Gustavo Ignacio Vázquez Cervantes, Benjamín Pineda, Daniela Ramírez Ortega, Alelí Salazar, Dinora Fabiola González Esquivel, Daniel Rembao, Sergio Zavala Vega, Saúl Gómez-Manzo, Gonzalo Pérez de la Cruz, Verónica Pérez de la Cruz
water. cDNA was obtained using the
First Strand cDNA Synthesis
System for quantitative
RT-PCR
(
RT-qPCR
) (
NP100042
;
OriGene
, Rockville, MD, USA) according to the manufacturer’s specifications. The cDNA was kept at −20
(16)
Neuroprotection by Wld
S
depends on retinal ganglion cell type and age in glaucoma
Molecular neurodegeneration
June 5, 2021
Risner ML, Pasini S, McGrady NR, D'Alessandro KB, Yao V, Cooper ML, Calkins DJ.
(17)
17-Beta Hydroxysteroid Dehydrogenase 13 Deficiency Does Not Protect Mice From Obesogenic Diet Injury
Hepatology (Baltimore, Md.)
May 1, 2021
Ma Y, Brown PM, Lin DD, Ma J, Feng D, Belyaeva OV, Podszun MC, Roszik J, Allen JN, Umarova R,
et al
.
(18)
Rapid ATF4 Depletion Resets Synaptic Responsiveness after cLTP
eNeuro
May 1, 2021
Amar F, Corona C, Husson J, Liu J, Shelanski M, Greene L.
(19)
Interplay between tumor microenvironment and partial EMT as the driver of tumor progression
iScience
February 19, 2021
Aggarwal V, Montoya CA, Donnenberg VS, Sant S.
(20)
Microglia Regulate Cell Genesis in a Sex-dependent Manner in the Neonatal Hippocampus
Neuroscience
January 15, 2021
Nelson LH, Peketi P, Lenz KM.
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Reviews
Be the First to Write a Review!
First Strand cDNA Synthesis Kit from OriGene Technologies
Supplier Page
View Company Product Page
$540.00
Product Specs
Company Info
Citations
Reviews
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