Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 from Abcam

Product Specs
ItemMitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab115348
Quantity1 x 96 Tests
ApplicationsEnzyme activity assay
MethodFuncS
Sample TypeCell culture extracts, Tissue Extracts
Detection Range60 - 250 µg/ml
Assay Time4h 0m
Research AreaNeuroscience
NCBI Full Gene Namealdehyde dehydrogenase 3 family member A2
NCBI Gene AliasesALDH10, FALDH, SLS

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Formate Reduces Ischemic Injury in Female Hearts Lacking Alcohol Dehydrogenase 5FASEB BioadvDecember 12, 2025Haley Garbus-Grant, Obialunanma V. Ebenebe-Kasonde, Raihan Kabir, Mark J. Kohrwere then diluted in assay buffer and ALDH2 activity was assessed using a commercial colorimetric kit (ab115348; Abcam, Cambridge, MA) per the manufacturer's instructions. Activity was estimated by measuring the

(2) Oenological tannins mitigate rotenone-induced mitochondrial impairments and oxidative stress, with concomitant detection of urolithin A in the brainBiochemistry and Biophysics ReportsDecember 1, 2025Olga Wojciechowska, Michaël Jourdes, Mirosław Andrusiewicz, Małgorzata Pokrzywa, Marta Karaźniewicz-Łada, Jadwiga Jodynis-Liebert, Pierre-Louis Teissedre, Małgorzata Kujawskawas determined using a colourimetric Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348, Abcam, Amsterdam, NL). We followed the protocols provided by the manufacturer and used in our previous

(3) ALDH2 Ameliorates Acute Gouty Arthritis Through Inhibiting NLRP3 Inflammasome and Pyroptosis by Nrf2/ROS PathwayJ Inflamm ResAugust 14, 2025Shuting Tong, Xin Li, Fangying Wang, Qi Cheng, Peiyu Zhang, Mo Chen, Yifan Xie, Xiaoyong Lu, Huaxiang Wuobserved under a polarizing microscope. ALDH2 Activity Assay A mitochondrial ALDH2 activity assay kit (ab115348, Abcam) was used to detect ALDH2 enzyme activity in the cells. Briefly, adherent cells were scraped

(4) ALDH2 Ameliorates Acute Gouty Arthritis Through Inhibiting NLRP3 Inflammasome and Pyroptosis by Nrf2/ROS PathwayJ Inflamm ResAugust 14, 2025Shuting Tong, Xin Li, Fangying Wang, Qi Cheng, Peiyu Zhang, Mo Chen, Yifan Xie, Xiaoyong Lu, Huaxiang Wuobserved under a polarizing microscope. ALDH2 Activity Assay A mitochondrial ALDH2 activity assay kit (ab115348, Abcam) was used to detect ALDH2 enzyme activity in the cells. Briefly, adherent cells were scraped

(5) Design, Synthesis, and Study of Protective Activity Against Stroke for Novel Water-Soluble Aldehyde Dehydrogenase 2 ActivatorsMoleculesJuly 10, 2025Fengping Zhao, Zhenming Yu, Wei Tian, Xinhui Huang, Qingsen Zhang, Ruolan Zhou, Jian Hu, Shichong Yu, Xin Chen, Canhui ZhengEvaluation of the ALDH2 Activation Activity of Target Compounds The test employed the ALDH2 test kit (Abcam ab115348, Cambridge, UK) and the active human ALDH2 protein (Abcam ab87415). The testing principle is that ALDH2

(6) The Pathological Role and Therapeutic potential of ALDH2 in acrolein detoxification Following Spinal Cord Injury in MicebioRxivJune 8, 2025Siyuan Shi, Siyuan Herr, Seth Alford, Rachel Stingle, Zaiyang Stingel, Zaiyang Zhang, Timothy Dongcollected right after to evaluate the ALDH2 enzyme activity levels using a commercial colorimetric kit (#ab115348; Abcam) according to the manufacturer’s protocol and a previous publication. Briefly, tissue lysates

(7) Antrodia cinnamomea triterpenoids attenuate cardiac hypertrophy via the SNW1/RXR/ALDH2 axisRedox BiologyDecember 1, 2024Yinghua Ma, Yunxia Wang, Gulinigaer Anwaier, Nuerbiye Tuerdi, Yangchang Wu, Yinyue Huang, Boyang Qin, Haoyue Ma, Qiao Zhang, Dalei Wu, et al.Sakura Finetek Japan Co., Ltd. (Tokyo, Japan). Mitochondrial aldehyde dehydrogenase activity assay kit (ab115348) was purchased from Abcam (Cambridge, England). 4-Hydroxynonenal (4-HNE) ELISA Kit (H268) was purchased

(8) ALDH2 mediates the effects of sodium-glucose cotransporter 2 inhibitors (SGLT2i) on improving cardiac remodelingCardiovascular DiabetologyOctober 26, 2024Han Liu, Bingchen Jiang, Rui Hua, Xuehao Liu, Bao Qiao, Xiangxin Zhang, Xilong Liu, Wenjun Wang, Qiuhuan Yuan, Bailu Wang, et al.activity ALDH2 activity was measured with a Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348, Abcam). Immunofluorescence staining The samples were subsequently fixed with 4% paraformaldehyde

(9) Aldehyde dehydrogenase 2 preserves kidney function by countering acrolein-induced metabolic and mitochondrial dysfunctionJCI InsightOctober 8, 2024Szu-Yuan Li, Ming-Tsun Tsai, Yu-Ming Kuo, Hui-Min Yang, Zhen-Jie Tong, Hsiao-Wei Cheng, Chih-Ching Lin, Hsiang-Tsui Wangactivity in tissues or cells were evaluated using the Mitochondrial ALDH2 Activity Assay kit (Abcam, ab115348) and PK Assay Kit (Abcam, ab83432), respectively, following the manufacturer’s instructions as described

(10) Impaired Osteogenesis in Human Induced Pluripotent Stem Cells with Acetaldehyde Dehydrogenase 2 MutationsInternational journal of stem cellsAugust 30, 2024Lim J, Han H, Jung SI, Rim YA, Ju JH.assay ALDH2 activity was assessed using the Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348; Abcam) according to the manufacturer’s instructions. Cells were scraped and incubated with 1x extra-ction

(11) Dysregulation of cardiac mitochondrial aldehyde dehydrogenase 2: Studies in dogs with chronic heart failureJournal of Molecular and Cellular Cardiology PlusJune 28, 2024Ramesh C. Gupta, Vinita Singh-Gupta, Kristina J. Szekely, Kefei Zhang, David E. Lanfear, Hani N. Sabbahof phosphorylated mitochondrial fractions was prepared using 1× extraction buffer supplied by a kit (ab115348) of mitochondrial aldehyde dehydrogenase activity (Abcam Biotechnology company, Cambridge, United Kingdom).

(12) Coordinated action of a gut–liver pathway drives alcohol detoxification and consumptionNature MetabolismJune 20, 2024Yaojie Fu, Bryan Mackowiak, Yu-Hong Lin, Luca Maccioni, Taylor Lehner, Hongna Pan, Yukun Guan, Grzegorz Godlewski, Hongkun Lu, Cheng Chen, et al.concentration of 5 mg/mL. Next, the Mitochondrial aldehyde dehydrogenase (ALDH2) activity assay kit (Abcam; ab115348) was used to determine mitochondrial ALDH2 in liver samples by following manufacturer’s instructions,

(13) Mitochondrial ALDH2 improves ß‐cell survival and function against doxorubicin‐induced apoptosis by targeting CK2 signalingJournal of Diabetes InvestigationJune 1, 2024Udayakumar Karunakaran, Eun Yeong Ha, Suma Elumalai, Kyu Chang Won, Jun Sung Moonintensity was used. Following the experiment, an aldehyde dehydrogenase (ALDH2) activity assay kit (ab115348; Abcam) was utilized in accordance with the manufacturer's instructions to determine the ALDH2 activity

(14) Mitochondrial aldehyde dehydrogenase-2 coordinates the hydrogen sulfide - AMPK axis to attenuate high glucose-induced pancreatic β-cell dysfunction by glutathione antioxidant systemRedox BiologyFebruary 1, 2024Udayakumar Karunakaran, Suma Elumalai, Seung Min Chung, Kathrin Maedler, Kyu Chang Won, Jun Sung Moonthe NADPH/NADP+ ratio. After the experiment, the aldehyde dehydrogenase (ALDH2) activity assay kit (ab115348; Abcam) was used to determine the ALDH2 activity by measuring the absorbance at 450 nm, according to

(15) Estrogen-mediated mitigation of cardiac oxidative stress in ovariectomized rats is associated with upregulated cardiac circadian clock Per2 and heart-specific miRNAsLife SciencesOctober 15, 2023Syed Anees Ahmed, Baohong Zhang, Abdel A. Abdel-Rahmanactivity was determined using the Mitochondrial Aldehyde Dehydrogenase Activity Assay Kit (Catalog Number: ab115348; Abcam, Ann Arbor, MI, USA). In brief, the heart tissue samples were homogenized using phosphate-buffered

(16) Gene Therapy to Treat Osteopenia Associated With Chronic Ethanol Consumption and Aldehyde Dehydrogenase 2 DeficiencyJBMR PlusApril 1, 2023Anna E Camilleri, Michelle Cung, Fiona M Hart, Odelya E Pagovich, Ronald G Crystal, Matthew B Greenblatt, Katie M StilesRT‐qPCR. ALDH2 enzymatic activity in liver was quantified using a commercially available assay kit (Abcam; ab115348, Cambridge, MA, USA). Osteopenia in the femur was assessed by micro‐computed tomography (μCT). Serum

(17) Neuroprotective effects of Alda-1 mitigate spinal cord injury in mice: involvement of Alda-1-induced ALDH2 activation-mediated suppression of reactive aldehyde mechanismsNeural regeneration researchJanuary 1, 2022Khan M, Qiao F, Kumar P, Touhidul Islam SM, Singh AK, Won J, Singh I.activity was measured in tissue lysates using an ALDH2 enzymatic activity assay kit from Abcam (Cat# ab115348) according to the manufacturer’s protocol and described by Hua et al. (2018). As described in the western blot analysis

(18) GSNOR and ALDH2 alleviate traumatic spinal cord injuryBrain researchMay 1, 2021Khan M, Qiao F, Islam SMT, Dhammu TS, Kumar P, Won J, Singh AK, Singh I.activity in tissue lysates was measured using an ALDH2 enzymatic activity assay kit from Abcam (Cat# ab115348) according to the manufacturer’s protocol and as described by Hua et al. (Hua et al., 2018). As described

(19) Can Cranberry Juice Protect against Rotenone-Induced Toxicity in Rats?NutrientsMarch 24, 2021Kurpik M, Zalewski P, Kujawska M, Ewertowska M, Ignatowicz E, Cielecka-Piontek J, Jodynis-Liebert J.enzyme was determined using the ALDH2 activity assay kit according to the manufacturer’s protocol (ab115348, Abcam). Tissue samples of the brain were homogenized in three-volumes of ice-cold phosphate-buffered

(20) Brain ethanol metabolism by astrocytic ALDH2 drives the behavioural effects of ethanol intoxicationNature MetabolismMarch 22, 2021Shiyun Jin et al.homogenized, and ALDH2 enzyme activity was measured using the mitochondrial ALDH2 enzyme activity kit (ab115348, Abcam) following the manufacturer’s instructions. The prepared sample aliquots of 100 μl were added

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Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit from Abcam

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Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit from Abcam

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