Cyclic AMP ELISA Kit 581001 from Cayman Chemical

Product Specs
ItemCyclic AMP ELISA Kit
CompanyCayman Chemical
Price $1,586.00Supplier Page View Company Product Page
Catalog Number581001
Quantity480 Solid
ApplicationsELISA
Detection Range0.078-10 pmol/ml
Sensitivity80% B/B0: 0.1 pmol/ml
Research AreacAMP Signaling, Cell Biology, Cell Signaling
Source/Expression SystemAnimal/Bovine:Animal/Eel:Animal/Mouse:Animal/Rabbit
OriginUSA
Storage-20

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Specific GPCRs Elicit Unique Extracellular Vesicle MiRNA Array Signatures: An Exploratory StudyeLifeMarch 20, 2026Xiao Shi, Michelle C Palumbo, Sheila Benware, Jack Wiedrick, Sheila Markwardt, Aaron J Janowskymin before CCPA addition. cAMP accumulation was measured using a cAMP EIA kit from Cayman Chemical (581001, Ann Arbor, MI, USA), according to the manufacturer’s instructions. All the experiments were conducted

(2) ADT-030, a novel PDE10 inhibitor, demonstrates potent antitumor activity in pancreatic ductal adenocarcinomabioRxivFebruary 17, 2026Dhana Sekhar Reddy Reddy Bandi, Ganji Purnachandra Nagaraju, Sujith Sarvesh, Jeremy B. Foote, Adam B. Keeton, Xi Chen, Veronica Ramirez-Alcantara, Thomas Holmes, Mehmet Akce, Anju Singh, et al.intracellular cAMP and cGMP levels were measured. Enzyme immunoassay kits were used to detect cAMP (Cat# 581001, Cayman) and cGMP (Cat# 581021, Cayman) by following manufacturer’s instructions. The results were expressed

(3) Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammationCell ReportsJanuary 27, 2026Jun Nagai, Madeline M. Hastings, Airi Nishida, Sreyashi Majumdar, Kendall Zaleski, Sofia A. Marshall, Junrui Lin, Mira A.S. Saliman, Yumi Tani, Yamato Murakami, et al.ELISA,R&D,DY413 IL-33 mouse ELISA,R&,M3300 "Cyclic AMP ELISA Kit, 96 strip wells",Cayman Chemical,581001 Cysteinyl Leukotriene ELISA Kit,Cayman Chemical,500390 Prostaglandin D2-MOX ELISA Kit,Cayman Chemical,Item

(4) Adenosine/A 2A R/PKA signaling regulates HO-1-mediated anti-inflammatory responses during Leishmania donovani infectionmBioJanuary 14, 2026Tapasi Das, Pronay Brahmachari, Anindita UkilMouse TNF alpha ELISA Kit,Abcam,ab100747,ab100747, Mouse IL-12 p40 + IL-12 p70 ELISA Kit,Abcam,ab100699,ab100699, Cyclic AMP ELISA Kit,Cayman Chemical,581001,581001, Imprint Chromatin Immunoprecipitation Kit Protocol,Merck,CHP1,CHP1,CHP1 List of chemical

(5) Mitochondrial membrane junction-mediated ATP channeling drives activity-dependent glucose metabolismbioRxivDecember 19, 2025Dengbao Yang, Gemma Molinaro, Nadine Nijem, Chuanhai Zhang, Dylen Penny, Meijuan Bai, Mei-Jung Lin, Xiaodong Wen, Salvador Pena, Janaka Wansapura, et al.The cAMP levels of brown adipocytes were then measured using a Cyclic AMP ELISA kit (Cayman Chemical, 581001) following manufacturer’s protocol. To inhibit cAMP hydrolysis, 250 μM 3-isobutyl-1-methylxanthine (IBMX)

(6) Impaired cAMP–PKA–CREB1 signalling drives mitochondrial dysfunction in skeletal muscle during cancer cachexiaNature MetabolismNovember 12, 2025Elia Angelino, Lorenza Bodo, Roberta Sartori, Valeria Malacarne, Beatrice D’Anna, Nicolò Formaggio, Suvham Barua, Tommaso Raiteri, Andrea Lauria, Simone Reano, et al.ether and lyophilized. cAMP content was detected with the Cyclic AMP ELISA Kit (Cayman Chemical; item 581001) according to the manufacturer’s protocol. cAMP quantification was normalized over milligram of tissue

(7) The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier functionEMBO ReportsOctober 28, 2025Takako Ikeda, Yuki Masujima, Keita Watanabe, Akari Nishida, Mayu Yamano, Miki Igarashi, Nobuo Sasaki, Hironori Katoh, Ikuo Kimuratranscriptase,Invitrogen,28025013 3-isobutyl 1-methylxantine,Sigma,28822-58-4 cAMP EIA kit,Cayman Chemical,581001 Folskolin,Sigma,66575-29-9 Crystal violet,Wako,031-04852 Alexa Fluor 488,Invitrogen,A11008 "Goat

(8) The Legionella pneumophila Dot/Icm Type IV Secretion System is Structurally and Functionally Resilient in Absence of Species-specific Proteins Dis2 and Dis3Journal of Molecular BiologyOctober 1, 2025Jacquelyn R. Roberts, Arwen E. Frick-Cheng, Henry J. Styron, Clarissa L. Durie, Louise Chang, Melanie D. Ohiinfected U937 macrophages was measured using a cAMP ELISA (Cayman Chemical Cyclic AMP ELISA kit, Item No. 581001) performed according to manufacturer’s instructions. To prepare cell lysate all excess PBS was removed

(9) Specific GPCRs Elicit Unique Extracellular Vesicle MiRNA Array Signatures: An Exploratory StudybioRxivJune 21, 2025Xiao Shi, Michelle C. Palumbo, Sheila Benware, Jack Wiedrick, Sheila Markwardt, Aaron J Janowsky10 min before CCPA addition. cAMP accumulation was measured using cAMP EIA kit from Cayman Chemical (581001, Ann Arbor, MI), according to the manufacturer’s instructions. All the experiments were conducted with

(10) Acute regulation of murine adipose tissue lipolysis and insulin resistance by the TGFβ superfamily protein GDF3Nat CommunMay 13, 2025Nagasuryaprasad Kotikalapudi, Deepti Ramachandran, Daniel Vieira, William B. Rubio, Gregory R. Gipson, Luca Troncone, Kylie Vestal, David E. Maridas, Vicki Rosen, Paul B. Yu, et al.were lysed of 0.1 M HCl. Determination of cAMP was performed using cAMP ELISA Kit (Cayman chemicals, # 581001) following the manufacturer’s instructions. Generation of dual reporter cells Immortalized iWAT-SVF

(11) A novel phosphodiesterase inhibitor for the treatment of chronic liver injury and metabolic diseasesHepatologyApril 1, 2025Dalton W. Staller, Sanjali S. Panigrahi, Yahani P. Jayasinghe, Yuxiang Dong, Sohan Mahto, Virender Kumar, Donald R. Ronning, Ram I. Mahatohydroxyproline concentrations were measured with the Cyclic AMP ELISA Kit (Cayman Chemicals Item No. 581001) and Hydroxyproline Colorimetric Assay Kit (Abcam ab222941) according to the manufacturer’s instructions. 2.15

(12) PDE4 inhibitor rolipram represses hedgehog signaling via ubiquitin-mediated proteolysis of GLI transcription factors to regress breast cancerThe Journal of biological chemistryMarch 1, 2025Bagchi A, Bhattacharya A, Bera A, Basak D, Chatterji U, Biswas A.triplicate samples per treatment group. The assay kit was obtained from Cayman Chemicals (item no.: 581001), and the assay was performed following the manufacturer’s protocol after washing the cells with 1X

(13) SPTLC3 regulates plasma membrane sphingolipid composition to facilitate hepatic gluconeogenesisCell ReportsDecember 24, 2024David Montefusco, Maryam Jamil, Daniel Canals, Siri Saligrama, Yang Yue, Jeremy Allegood, L. Ashley Cowartby BCA, and then samples were stored at −80°C. Measurements were performed with an ELISA kit (Cayman 581001). Plasma membrane isolation Partially purified plasma membrane extracts were generated based on Suski

(14) Investigating the crosstalk between ABCC4 and ABCC5 in 3T3-L1 adipocyte differentiationFrontiers in Molecular BiosciencesDecember 9, 2024Ankit P. Laddha, Aniket Wahane, Raman Bahal, José E. Manautouintracellular cAMP levels Intracellular cAMP levels were analyzed using a cAMP ELISA kit (Catalog: 581001, Cayman Chemical, Ann Arbor, MI). 2 × 105 cells were seeded in 12 well plates. After 48 h of the last

(15) Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissueMolecular MedicineDecember 4, 2024Ewelina Palak, Donata Ponikwicka-Tyszko, Kamila Pulawska-Moon, Maria Sztachelska, Gabriela Milewska, Beata Modzelewska, Tomasz Kleszczewski, Maria L. Koivukoski, Piotr Bernaczyk, Hady Razak Hady, et al.FSH-stimulated cAMP production was evaluated using a cyclic AMP ELISA Kit (Cayman Chemical, MI, USA, cat. no. 581001). RNA isolation and gene expression Total RNA was extracted from the cells using the TRIzol/chloroform

(16) Membrane progesterone receptors mediate progesterone-stimulated glycogenolysis in the bovine uterine epitheliumReproductionDecember 1, 2024Malia D Berg, Matthew Deanwas imaged using FluorChem M (Protein Simple, CA, USA). cAMP ELISA A cyclic AMP (cAMP) ELISA kit (581001, Cayman Chemical Company) was used to determine the level of cAMP in BUTE cells. The manufacturer’s

(17) MSCs-derived HGF alleviates senescence by inhibiting unopposed mitochondrial fusion-based elongation in post-acute kidney injuryStem cell research & therapyNovember 19, 2024Zhuang K, Wang W, Zheng X, Guo X, Xu C, Ren X, Shen W, Han Q, Feng Z, Chen X, et al.supernatants were used to measure intracellular cyclic AMP (cAMP) levels using the cAMP ELISA Kit (581001, Cayman Chemical) following the manufacturer's instructions. Measurement of HGF secretion Briefly,

(18) CFTR Inhibitors Display Antiviral Activity against Herpes Simplex VirusVirusesAugust 16, 2024Ping Jiang, Zhong Dai, Chan Yang, Liqiong Ding, Songshan Li, Xinfeng Xu, Chen Cheng, Jinshen Wang, Shuwen Liupurchased from Rising Biotechnology Co., LTD (Quanzhou, Fujian, China). The Cyclic AMP ELISA Kit (Item No. 581001) was purchased from Cayman Chemical (Ann Arbor, MI, USA). A SGK1 Kinase Enzyme System was purchased

(19) Phosphodiesterase Inhibition to Sensitize Non-Small-Cell Lung Cancer to Pemetrexed: A Double-Edged StrategyCancersJuly 6, 2024Ivanina Foureau AV, Foureau DM, McHale CC, Guo F, Farhangfar CJ, Mileham KF.concentration of cAMP/cGMP was determined using a cAMP/cGMP kit according to manufacturer’s recommendations (#581001/#581021, Cayman Biochemical, Ann Arbor, MI, USA). In brief, samples were acetylated using 4 M KOH and

(20) Baicalin reduces chronic stress-induced breast cancer metastasis via directly targeting β2-adrenergic receptorJournal of Pharmaceutical AnalysisJuly 1, 2024Qi Jia, Yinyin Zhou, Li Song, Ximeng Shi, Xuan Jiang, Ruizhi Tao, Aiyun Wang, Yuanyuan Wu, Zhonghong Wei, Yinan Zhang, et al.quantification Intracellular levels of cAMP were quantified colorimetrically using a cAMP ELISA kit (No. 581001, Cayman Chemical, Ann Arbor, MI, USA). Results are presented as pmol of cAMP per mL of protein. To determine

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Cyclic AMP ELISA Kit

Cyclic AMP ELISA Kit

Cyclic AMP ELISA Kit from Cayman Chemical

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Cayman Chemical

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Cyclic AMP ELISA Kit from Cayman Chemical

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