Description
Cayman’s PAD4 Inhibitor Screening Assay Kit (Ammonia) provides a convenient method for screening human PAD4 inhibitors. PAD4 deiminates N-α-benzoyl-L-arginine ethyl ester (BAEE), a non-natural substrate with similar kinetic properties to the natural substrates, producing ammonia.3 Ammonia reacts with a detector resulting in a fluorescent product. Fluorescence is then analyzed with an excitation wavelength of 405-415 nm and an emission wavelength of 470-480 nm