TUNEL Assay Kit - FITC ab66108 from Abcam

Product Specs
ItemTUNEL Assay Kit - FITC
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab66108
Quantity50 Test
MethodFlow Cyt
Sample TypeAdherent cells, Suspension cells
Assay Time2h 30m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Unveiling the role of PSD95 phosphorylation after traumatic brain injury: insights from phosphoproteomic analysisBMC NeurologyJuly 30, 2025Zhongxiang Zhang, Junpeng Gao, Gang Li, Jin Cheng, Jiangtao Yu, Pengcheng Wang, Ruining Liu, Cheng Jiang, Haoli Ma, Yan Zhaohistochemistry After deparaffination and hydration, tissue sections were stained using a commercial TUNEL assay kit (Ab66108, Abcam, United Kingdom) according to the manufacturer’s instructions. Nuclear staining was performed

(2) Reciprocal regulation of GPNMB/HIF-1α for Inhibition of neuronal ferroptosis in delayed encephalopathy after acute carbon monoxide poisoningActa Neuropathol CommunJuly 14, 2025Zuolong Liu, Lanyue Sun, Nan Gao, Wei Li, Li Pang24 h) as described [32]. TUNEL staining DNA fragmentation was assessed using TUNEL Assay Kit-FITC (ab66108, Abcam) or TUNEL Assay-Kit-HRP-DAB (ab206386, Abcam). Briefly, PC-12 cells were fixed with 1% paraformaldehyde

(3) RDM1 plays an oncogenic role in clear cell renal cell carcinoma potentially by modulating MCM2Sci RepJuly 2, 2025Xiuming Li, Hui Liu, Yujie Wei, Anxin Wang, Chengcheng Lv, Yu Zengscores: low (0–3) or high (4–12) expression. TUNEL assay Through using the TUNEL Assay Kit-FITC (ab66108, Abcam), the TUNEL assay was conducted for detecting the late apoptotic cells following specific protocols.

(4) Optimizing Cryopreservation: The Effect of MitoTEMPO and Glycerol Ratios on Ram Semen Quality After Freezing and ThawingVet Med SciJune 30, 2025Recep Hakkı Koca, İbrahim Halil Güngör, Aslıhan Çakır Cihangiroğlu, Tutku Can Acısu, Nida Badıllı, Serkan Ali Akarsu, Şeyma Özer Kaya, Emre Kaya, Gaffari Türk, Mustafa Sönmez, et al.method was used, and analysis was performed with the commercial kit for this method, Apodirect (ABCAM ab66108, Cambridge, England). Briefly, the negative (N) and positive (P) controls included in the kit package

(5) ALKBH5 modulates m6A modification to enhance acute myeloid leukemia resistance to adriamycinBiomolecules and BiomedicineMay 1, 2025Yonghua Liu, Jinhong Jiang, Yuxiao Zeng, Yu Jiangresuspended in 0.5 mL phosphate-buffered saline (PBS) and processed according to the TUNEL kit instructions (ab66108, Abcam Inc., Cambridge, MA, USA). Cells were imaged under a fluorescence microscope (LSM700B, Zeiss,

(6) Cholesterol Targeted Catalytic Hydrogel Fueled by Tumor Debris can Enhance Microwave Ablation Therapy and Anti-Tumor Immune ResponseAdvanced science (Weinheim, Baden-Wurttemberg, Germany)February 1, 2025Shen L, Yang Z, Zhong Y, Bi Y, Yu J, Lu Q, Su Y, Chen X, Zhao Z, Shu G, et al.(Invitrogen Antibodies, catalog: 14‐5773‐82), Ki67 (Abcam, catalog: ab15580) and TUNEL (Abcam, catalog: ab66108) were used to measure protein expression and localization after different treatments. The supernatant

(7) Lipid peroxidation products induce carbonyl stress, mitochondrial dysfunction, and cellular senescence in human and murine cellsAging cellJanuary 1, 2025Monroe TB, Hertzel AV, Dickey DM, Hagen T, Santibanez SV, Berdaweel IA, Halley C, Puchalska P, Anderson EJ, Camell CD, et al.were assessed using a commercial kit to fluorescently tag 3′‐hydroxyl ends of DNA fragments (Abcam, ab66108). Briefly, IMR90s treated with vehicle and 4‐HNE continuously for 1 week were fixed with formaldehyde

(8) Cellular effects of splenectomy on liver regeneration after 70% resectionFrontiers in cell and developmental biologyJanuary 1, 2025Elchaninov A, Vishnyakova P, Gantsova E, Chirkova M, Karyagina V, Anatoliy L, Kananykhina E, Kuznetsova M, Atabekov I, Karpulevich E, et al.least 3,000 cells counted for each marker. TUNEL-test Test was performed using TUNEL assay kit-FITC (ab66108, Abcam, UK). Cell apoptosis was assessed by counting the number of TUNEL-positive cells (green) in the

(9) Anticancer effect of the oncolytic Newcastle disease virus harboring the PTEN gene on glioblastomaOncology lettersJanuary 1, 2025Kim S, Jung BK, Kim J, Jeon JH, Kim M, Jang SH, Kim CS, Jang H.TUNEL assay was performed according to the manufacturer's instructions (TUNEL Assay Kit-FITC; cat. no. ab66108; Abcam). Following fixation, the cells were permeabilized with 0.1% Triton X-100 in PBS for 2 min on

(10) Palindromic carbazole derivatives: unveiling their antiproliferative effect via topoisomerase II catalytic inhibition and apoptosis inductionJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2024Mateusz Olszewski, Natalia Maciejewska, Anoop Kallingal, Agnieszka Chylewska, Aleksandra M. Dąbrowska, Małgorzata Biedulska, Mariusz Makowski, José M. Padrón, Maciej Baginskireference. DNA fragmentation analysis A549 and HCT-116 cells were tested using TUNEL Assay Kit – FITC (Abcam, #ab66108, Cambridge, UK). After drug treatment, samples were harvested using trypsin solution, washed with PBS,

(11) Gene discovery from microbial gene libraries I: protection against reactive oxygen species-driven DNA damageMicrobiology SpectrumNovember 5, 2024John C. Taylor, Carmen Gu Liu, James D. Chang, Brianna E. Thompson, Anthony W. Maressocentrifuged at 5,000 × g for 10 minutes, followed by treatment with a TUNEL assay kit (Abcam, cat# ab66108). Fluorescence intensity was observed at Ex/Em = 488 nm/520 nm. Amplex Red Amplex Red analysis was

(12) Molecular mechanism of ALKBH5‐mediated m6A demethylation regulating lipopolysaccharide‐induced epithelial–mesenchymal transition in sepsis‐induced acute kidney injuryThe Kaohsiung Journal of Medical SciencesNovember 1, 2024Hai‐Hong Zhao, Chun‐Ling Chen, Fen‐Fang Chen, Lu‐Lu Zhang, Mei‐Mei Li, Ze‐Bao Heresuspended in 0.5 mL of phosphate‐buffered saline (PBS), and apoptosis was evaluated via a TUNEL assay kit (ab66108, Abcam, Cambridge, MA, USA). The cells were imaged under a fluorescence microscope (LSM700b, Zeiss,

(13) Myeloid beta-arrestin 2 depletion attenuates metabolic dysfunction-associated steatohepatitis via the metabolic reprogramming of macrophagesCell MetabolismOctober 1, 2024Xiaoli Wei, Dongqing Wu, Jing Li, Miaomiao Wu, Qianhui Li, Zhaodi Che, Xu Cheng, Qianying Cheng, Fan Yin, Hao Zhang, et al.Kit,Applygen,Cat#E1026 Total cholesterol (TC) enzymatic Determination Kit,Applygen,Cat#E1015 TUNEL assay kit,Abcam,Cat#ab66108 Chemiluminescence (ECL) substrate kit,Thermo,Cat#A38554 Mouse IL-1β ELISA assay kit,Dakewe,Cat#1210122 Mouse

(14) Neural stem cells derived from α-synuclein-knockdown iPS cells alleviate Parkinson's diseaseCell death discoverySeptember 17, 2024Wang CH, Lin GC, Fu RH, Huang YC, Chen SY, Lin SZ, Harn HJ, Shyu WC, Huang YF, Jeng LB, et al.determined using ImageJ software by staining the frozen sections with the TUNEL assay kit (Abcam, Cat. No. ab66108). Statistical analysis Data were obtained from at least three independent experiments and compared

(15) Monolayer, open-mesh, pristine PEDOT:PSS-based conformal brain implants for fully MRI-compatible neural interfacesBiosensors and BioelectronicsSeptember 15, 2024Jung-Hoon Hong, Ju Young Lee, Ankan Dutta, Sol Lip Yoon, Young Uk Cho, Kyubeen Kim, Kyowon Kang, Hyun Woo Kim, Dae-Hee Kim, Jaejin Park, et al.death of the brain by implanted probes. TUNEL assay was performed according to the manual (TUNEL-FITC, ab66108, Abcam, Cambridge, UK). Sections were washed 3 times for 10 min, followed by 2 times treatments for

(16) Dexmedetomidine and argon in combination against ferroptosis through tackling TXNIP-mediated oxidative stress in DCD porcine liversCell Death DiscoveryJuly 11, 2024Qian Chen, Jiashi Sun, Xiangfeng Liu, Zhigang Qin, Jieyu Li, Jianbo Ma, Zhengwei Xue, Yirong Li, Ziheng Yang, Qizhe Sun, et al.instructions of the manufacturer. TUNEL assay The sections were detected with in situ TUNEL assay (Abcam, ab66108, Cambridge, UK. TUNEL+ nuclei were visualised by green FITC fluorescence. Immunohistochemistry For

(17) A 70% Ethanol Neorhodomela munita Extract Attenuates RANKL-Induced Osteoclast Activation and H2O2-Induced Osteoblast Apoptosis In VitroMoleculesApril 11, 2024Seongtae Jeong, Il-Kwon Kim, Hanbyeol Moon, Hojin Kim, Byeong-Wook Song, Jung-Won Choi, Sang Woo Kim, Seahyoung Lee, Dong-Sik Chae, Soyeon LimKorea). 4.12. TUNEL-Propidium Iodide (PI) Assay The In situ Direct DNA Fragmentation (TUNEL) assay kit (ab66108; Abcam, Waltham, MA, USA) was employed to identify apoptotic cells, following the manufacturer’s instructions.

(18) Gasdermin‐E‐Dependent Non‐Canonical Pyroptosis Promotes Drug‐Induced Liver Failure by Promoting CPS1 deISGylation and DegradationAdvanced ScienceApril 1, 2024Shen‐Xi Ouyang, Jia‐Hui Zhu, Qi Cao, Jian Liu, Zhen Zhang, Yan Zhang, Jing‐Wen Wu, Si‐Jia Sun, Jiang‐Tao Fu, Yi‐Ting Chen, et al.transferase–mediated deoxyuridine triphosphate nick‐end labeling (TUNEL) assay was performed with a commercial kit (ab66108) according to the manufacturer's instructions (Abcam). Images were obtained by fluorescence microscope

(19) Viral proteins activate PARIS-mediated tRNA degradation and viral tRNAs rescue infectionbioRxivFebruary 29, 2024Nathaniel Burman, Svetlana Belukhina, Florence Depardieu, Royce A. Wilkinson, Mikhail Skutel, Andrew Santiago-Frangos, Ava B. Graham, Alexei Livenskyi, Anna Chechenina, Natalia Morozova, et al.harvested by centrifugation and treated according to the standard protocol (TUNEL Assay Kit – FITC, ab66108, Abcam). Measurement of FITC fluorescence was performed with CytoFLEX cytometer (Beckman Coulter) in

(20) Diallyl disulfide induces DNA damage and growth inhibition in colorectal cancer cells by promoting POU2F1 ubiquitinationInternational Journal of Biological SciencesJanuary 21, 2024Longzheng Xia, Jinguan Lin, Mingjing Peng, Xianjie Jiang, Qiu Peng, Shiwen Cui, Wenlong Zhang, Shizhen Li, Jiewen Wang, Linda Oyang, et al.cells was determined by terminal dUTP transferase nick end labeling (TUNEL) assay using a specific kit (ab66108, Abcam), per the manufacturer's protocol. Briefly, after the DADS treatment, the CRC cells were fixed

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TUNEL Assay Kit - FITC from Abcam

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TUNEL Assay Kit - FITC from Abcam

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