Fig 1: CeNGEN pyk expression pattern in the neurons of C. elegans, ATP levels and ATP utilization in Wildtype and ogt-1 animals.(A) pyk-1 expression analysis in neuronal cell, single cell neuronal RNA sequencing (RNA-seq) data from worm base (https://wormbase.org/species/c_elegans/gene/WBGene00009126#0-9fce6b37d81-10) was used to generate the image. (B) pyk-2 expression analysis in neuronal cell, as for pyk-1. (C) Relative amount of ATP measured using ATP assay kit (Abcam, cat# Ab83355) in whole worm lysate. (D) Relative amount of pyrophosphate (PPi) measured using pyrophosphate assay kit (Abcam, cat# Ab112155) in whole worm lysate. All data shown in ± SEM, analytical methods, student t-test was used *p <0.05, **p <0.01, ***p <0.001.