Intracellular glutathione (GSH) Detection Assay Kit ab112132 from Abcam

Product Specs
ItemIntracellular glutathione (GSH) Detection Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112132
Quantity100 Test
MethodFlow Cyt
Sample TypeAdherent cells, Suspension cells
Research AreaMetabolism

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(1) Urinary exosomes aggravate diabetic kidney disease by inducing podocyte ferroptosis via the miR-217/SIRT1/Nrf2 pathwayJ Cell Commun SignalApril 25, 2026Xin Du, Yubo Jiang, Hongbin Guo, Haiying Zhang, Shaoqing Wang, Hao Deng, Xinyi Huang, Xiu LiPeroxidation MDA Assay Kit (S0131S, Solarbio) and intracellular glutathione (GSH) Detection Assay Kit (ab112132, Abcam) were used to analyze the levels of GSH and MDA. 2.7 Intracellular Fe2+ level Intracellular

(2) Neuroprotective effects of rutin, sodium selenite, and rutin-conjugated selenium nanoparticles in a social isolation modelFront PharmacolApril 15, 2026Khaled M. Alam-ElDein, Abanoub A. S. Shaker, Manal F. El-Khadragy, Ibrahim K. M. Alabbadi, Ahmed E. Abdel Moneim, Alzahraa A. Elhemiely, Nievin Ahmed Mahran, Triveena M. Ramsis, Shaimaa H. El-Fayoumi, Mohamed A. Ali, et al.Cambridge, UK) (Misra and Fridovich, 1972; Aebi, 1984), while the concentration of reduced GSH (Cat. No. ab112132, Abcam, Cambridge, UK) a key indicator of cellular redox equilibrium was measured following Ellman’s

(3) Damage-induced pyroptosis drives endogenous thymic regeneration by activating the purinergic receptor P2Y2Cell Death & DiseaseJanuary 3, 2026Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Erin Kirsche, Makya Warren, Paul deRoos, Antonella Cardinale, Lorenzo Iovino, David Granadier, Colton W. Smith, et al.Superoxide Indicator (ThermoFisher, M36008), and Intracellular Glutathione (GSH) Detection Assay Kit (Abcam, ab112132). Thymocytes were isolated from untreated and TBI-treated mice and intracellular pyruvate and lactate

(4) Hypoxia Triggers ALYREF ‐Mediated m 5 C Methylation of KIF20A to Activate KIF20A / BUB1 for Generating Ferroptosis Resistance in Cervical Cancer CellsThe Kaohsiung Journal of Medical SciencesJanuary 1, 2026Yan Gao, Ting Zouassay kit (Abcam, Cambridge, UK, ab83366), MDA assay kit (Abcam, ab118970), and GSH assay kit (Abcam, ab112132), respectively, strictly according to the manufacturer's manuals. 2.5 Real‐Time Quantitative Polymerase Chain Reaction

(5) Targeting HCG18 counteracts ferroptosis resistance via blocking the miR-30a-5p/RRM2/GSS pathway in hepatocellular carcinomaInt J Biol SciMarch 21, 2025Tian Zhan, Yawei Liu, Shuoke Duan, Chen Lu, Heng Jia, Ming Jin, Jie Li, Xinru Du, Sizheng Sun, Yuan Li, et al.Intracellular GSH detection assay The concentration of GSH was determined using the GSH detection assay kit (ab112132, Abcam). First, cells were counted and reseeded in 24-well plates with 2 ×104 cells in per well and

(6) The LKB1–AMPK Signaling Axis Modulates Ferroptosis in Fibroblast-Like Synoviocytes Derived from Rheumatoid ArthritisBiomedicinesJanuary 30, 2025Ha-Reum Lee, Su-Jin Yoo, Jinhyun Kim, Seong Wook KangDetermination Cells were stained for 30 min at 37 °C using an Intracellular GSH Detection Assay kit (Cat# ab112132, Abcam, Cambridge, UK) following the manufacturer’s instructions. The cells were then washed in PBS

(7) The Protective Effects of Annexin A1 in Acute Lung Injury Mediated by Nrf2Immun Inflamm DisJanuary 14, 2025Hui Huang, Yuqin Shi, Yuequan Zhou(#BC5165, Solarbio, China). Glutathione peroxidase (GSH) activity was measured using a commercial kit (#ab112132, Abcam, USA). All procedures were performed according to the manufacturers’ instructions. 2.8 RT‐PCR Assay

(8) Inhibition of Glutamate‐to‐Glutathione Flux Promotes Tumor Antigen Presentation in Colorectal Cancer CellsAdvanced ScienceOctober 31, 2024Tao Yu, Kevin Van der Jeught, Haiqi Zhu, Zhuolong Zhou, Samantha Sharma, Sheng Liu, Haniyeh Eyvani, Ka Man So, Naresh Singh, Jia Wang, et al.GSH levels were measured using the Intracellular glutathione (GSH) Detection Assay Kit (Abcam, Cat#: ab112132), ROS levels were measured using the Cellular ROS/Superoxide Detection Assay Kit (Abcam, Cat#: ab139476)

(9) Tanshinone IIA suppresses ferroptosis to attenuate renal podocyte injury in diabetic nephropathy through the embryonic lethal abnormal visual‐like protein 1 and acyl‐coenzyme A synthetase long‐chain family member 4 signaling pathwayJournal of Diabetes InvestigationAugust 1, 2024Shuai Zhu, Zhiqiang Kang, Fengjiao ZhangMDA and GSH using an Iron Assay Kit (ab83366; Abcam), MDA Assay Kit (S0131S; Beyotime) and GSH Kit (ab112132; Abcam). For ROS level, the diacetyldichlorofluorescein diacetate staining cellular ROS assay kit (ab113851;

(10) Dual Stimuli-Responsive Nanoprecursor of Ascorbic Acid and Quinone Methide Disrupting Redox Homeostasis for Cancer TreatmentACS OmegaJuly 23, 2024Anup Dey, Pramod Kumar E. K., Chan Ho Kim, Yuce Li, Jae Hyung Parklevel The intracellular GSH level was determined by flow cytometry using an intracellular GSH assay kit (ab112132, Abcam, USA). The HT29 and L929 cells (2 × 105 cells) were seeded in a six-well plate for 24 h and incubated

(11) The Bioactivity of a Hydroxytyrosol-Enriched Extract Originated after Direct Hydrolysis of Olive Leaves from Greek CultivarsMoleculesJanuary 6, 2024Maria Kourti, Zoi Skaperda, Fotios Tekos, Panagiotis Stathopoulos, Christina Koutra, Alexios Leandros Skaltsounis, Demetrios Kouretasintracellular ROS and GSH levels using the DCF-DA and intracellular glutathione (GSH) Detection Assay Kit (ab112132; Abcam, Cambridge, UK), respectively. Furthermore, 200,000 cells/well were seeded in two separate 6-well

(12) Antioxidant and DNA-Protective Activity of an Extract Originated from Kalamon Olives DebitteringAntioxidants (Basel)January 31, 2023Maria Kourti, Maria V. Alvanou, Zoi Skaperda, Fotis Tekos, Georgios Papaefstathiou, Panagiotis Stathopoulos, Demetrios Kouretaswere assessed by flow cytometry, using DCF-DA and Intracellular glutathione (GSH) Detection Assay Kit (ab112132; Abcam, Cambridge, UK), respectively. 200,000 cells/well were seeded in two separate 6-well plates,

(13) Damage-induced pyroptosis drives endogenous thymic regeneration via induction of Foxn1 by purinergic receptor activationbioRxivJanuary 25, 2023Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Antonella Cardinale, Lorenzo Iovino, Paul deRoos, Kayla S. Hopwo, Colton W. Smith, David Granadier, Lucas B. Sullivan, et al.Superoxide Indicator (ThermoFisher, M36008), and Intracellular glutathione (GSH) Detection Assay Kit (Abcam, ab112132). Thymocytes were isolated from untreated and TBI-treated mice and intracellular pyruvate and lactate

(14) Echinatin mitigates sevoflurane-induced hippocampal neurotoxicity and cognitive deficits through mitigation of iron overload and oxidative stressPharmaceutical BiologyDecember 31, 2022Zilong Xu, Yanqiu You, Qiuqin Tang, Hui Zeng, Tianshou Zhao, Juan Wang, Fujun Lidetermining the levels of LDH (ab102526; Abcam, Cambridge, MA, USA), MDA (ab118970; Abcam), and GSH (ab112132; Abcam) by matching commercial kits. All operation steps refer to the instructions of these kits. Terminal

(15) HJ11 decoction restrains development of myocardial ischemia-reperfusion injury in rats by suppressing ACSL4-mediated ferroptosisFrontiers in PharmacologyNovember 22, 2022Fangyuan Zhang, Ziyun Li, Ping Gao, Jiaxi Zou, Yuting Cui, Yi Qian, Renjun Gu, Weiming Xu, Jingqing HuCambridge, United Kingdom), SOD Assay Kit (ab65354; Abcam, Cambridge, United Kingdom), and GSH Assay Kit (ab112132; Abcam, Cambridge, United Kingdom). The detection procedure was followed strictly according to the manufacturers’

(16) NKT cells adopt a glutamine-addicted phenotype to regulate their homeostasis and functionCell reportsOctober 25, 2022Kumar A, Yarosz EL, Andren A, Zhang L, Lyssiotis CA, Chang CH.kit,Sigma,Cat# MAK054-1KT Glutamine/glutamate assay kit,Promgea,Cat# J8021 GSH Detection,Abcam,Cat# ab112132 Cell Titer-Glo Luminescent Cell Viability,Promega,Cat# G7570 EasySep Mouse Naïve CD4+Tcell Iso Kit,STEM CELL

(17) Self-immolative nanosensitizer for glutathione depletion- assisted sonodynamic therapyTheranosticsOctober 24, 2022Chan Ho Kim, Dong Gil You, Pramod Kumar E. K., Kyung Hee Han, Wooram Um, Jeongjin Lee, Jae Ah Lee, Jae Min Jung, Heegun Kang, Jae Hyung Parkwashing twice with DPBS, the cells were stained with an intracellular GSH detection assay kit (Abcam, ab112132, Cambridge, UK), according to the manufacturer's instructions. The fluorescence intensity was measured

(18) MicroRNA-147a Targets SLC40A1 to Induce Ferroptosis in Human GlioblastomaAnalytical Cellular PathologyJuly 30, 2022Peng Xu, Fei-Hang Ge, Wen-Xin Li, Zhen Xu, Xue-Li Wang, Jing-Lan Shen, Ai-Bo Xu, Rong-Rong Hao12-HETE ELISA Kit (#133034), 15-HETE ELISA Kit (#133035), and glutathione (GSH) detection assay kit (#ab112132) were purchased from Abcam (Cambridge, UK). Adenosine 5'-triphosphate (ATP) detection assay kit (#S0026)

(19) Cdc25A inhibits autophagy-mediated ferroptosis by upregulating ErbB2 through PKM2 dephosphorylation in cervical cancer cellsCell death & diseaseNovember 6, 2021Wang C, Zeng J, Li LJ, Xue M, He SL.detection assay Intracellular GSH levels were measured with a standard GSH detection assay kit (Abcam, ab112132, UK) according to the manufacturer’s instructions. Cultured or transfected cells following drug treatments

(20) Small size gold nanoparticles enhance apoptosis-induced by cold atmospheric plasma via depletion of intracellular GSH and modification of oxidative stressCell Death DiscoveryDecember 1, 2020Paras Jawaid, Mati Ur Rehman, Qing-Li Zhao, Masaki Misawa, Kenji Ishikawa, Masaru Hori, Tadamichi Shimizu, Jun-ichi Saitoh, Kyo Noguchi, Takashi Kondocells containing intracellular ROS. Assessment of intracellular GSH Glutathione (GSH) kit (Abcam; ab112132) was used to measure intracellular GSH. Cells were exposed to He-CAP with or without the presence of

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Intracellular glutathione (GSH) Detection Assay Kit from Abcam

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Intracellular glutathione (GSH) Detection Assay Kit from Abcam

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