Cell Cytotoxicity Assay Kit (Colorimetric) ab112118 from Abcam

Product Specs
ItemCell Cytotoxicity Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112118
Quantity1000 Test, 5000 Tests
MethodFuncS
Sample TypeAdherent cells, Suspension cells

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Website: http://www.abcam.com

(1) Hypoxia Supports LPS-Driven Tolerance and Functional Activation in BV-2 Microglial CellsBiology (Basel)October 28, 2025Alicia Chavero Vargas, Natascha Köstlin-Gille, Reinhard Bauer, Stefanie Dietz-Ziegler, Anita S. Lokaj, Soumya Lutterbach, Christian Gille, Trim Lajqiincubated overnight. Cytotoxicity was evaluated using a colorimetric Cell Cytotoxicity Assay Kit (Cat. No. ab112118, Abcam). 20 µL of assay solution was added to each well, and the plate was incubated at 37 °C with 5%

(2) Isolation of Primary Human Saphenous Vein Endothelial Cells, Human Internal Thoracic Artery Endothelial Cells, and Human Adipose Tissue-Derived Microvascular Endothelial Cells from Patients Undergoing Coronary Artery Bypass Graft SurgeryInternational Journal of Molecular SciencesSeptember 21, 2025Daria Shishkova, Yulia Yurieva, Alexey Frolov, Vera Matveeva, Evgenia Torgunakova, Victoria Markova, Anastasia Lazebnaya, Anton Kutikhin(703001, Wuxi NEST Biotechnology Co., Ltd., Wuxi, China);Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118, Abcam, Cambridge, UK);16-well E-Plates for the xCELLigence Real-Time Cell Analysis Dual Purpose Instrument

(3) Biocompatible nanocomposite cryogels with improved mechanical properties based on polyvinyl alcohol and carbon nanotubes for cardiovascular applicationsFrontiers in Bioengineering and BiotechnologySeptember 1, 2025Maria A. Rezvova, Tatiana V. Glushkova, Kirill Yu Klyshnikov, Alexey L. Pykin, Tatiana B. Tkachenko, Tatiana N. Akentieva, Alexander E. Kostyunin, Pavel S. Onishchenko, Natalia N. Borisova, Marina P. Fokeeva, et al.activity was evaluated using a colorimetric method with the Cell Cytotoxicity Assay Kit - Colorimetric (ab112118, Abcam, Cambridge, United Kingdom). The reagent, diluted to its working concentration (1:5 with the

(4) Biologically Active Implants Prevent Mortality in a Mouse Sepsis ModelAdvanced Healthcare MaterialsAugust 15, 2025Martin Stark, Fereshteh Bayat, Sara Rahmani, Mathura Thirugnanasampanthar, Taylor Kramer, Elise Schwarz, David Wilson, Jeffrey I. Weitz, Zeinab Hosseinidoust, Tohid F. Didarwere purchased from Thermo Fisher Scientific (ON, CA). Phalloidin FITC Reagent, Cytotoxicity Assay (ab112118), and Alkaline Phosphatase Assay Kit (ab83369) were purchased from Abcam (abcam.com). Pseudomonas Aeruginosa

(5) 4-Phenyl Butyric Acid (4-PBA) Suppresses Neutrophil Recruitment in a Murine Model of Acute Perinatal InflammationJ Immunol ResJuly 7, 2025Christian Gille, Maylis Jungwirth, Silvia Pezer, Stefanie Dietz-Ziegler, Natascha Köstlin-Gille, Trim Lajqicytotoxicity of the stressors 4-PBA and LPS, we used the colorimetric Cell Cytotoxicity Assay Kit (#ab112118, Abcam, Cambridge, UK). 10.000 cells per well were plated in a 96-well plate and treated as described

(6) Fiber and polyphenol enriched biscuits using date palm byproduct: Physiochemical characteristics, sensory properties, in vitro digestion, and storage stabilityJournal of Food ScienceJanuary 19, 2025Meththa Ranasinghe, Mariam Alghaithi, Priti Mugdil, Balan Sundarakani, Constantinos Stathopoulos, Sajid Maqsoodin the same conditions as above for 72 h. Then, prewarmed cell cytotoxicity assay solutions (Abcam ab112118) were added (20 µL) followed by an incubation of 5 h. The absorbance was measured at 570 and 605 nm

(7) Valorizing date seeds through ultrasonication to enhance quality attributes of dough and biscuit: Part 2 – Study on bioactive properties, sensory acceptance, in vitro gastrointestinal digestion and shelf life of biscuitsUltrasonics SonochemistryJanuary 1, 2025Meththa Ranasinghe, Mariam Alghaithi, Constantinos Stathopoulos, Balan Sundarakani, Sajid Maqsoodsame conditions for 72 h. Subsequently, 20 μL of prewarmed cell cytotoxicity assay solutions (Abcam ab112118) were introduced, followed by a 5 h incubation period. Absorbance readings were taken at 570 and 605

(8) A microscale soft lithium-ion battery for tissue stimulationNature Chemical EngineeringOctober 25, 2024Yujia Zhang, Tianyi Sun, Xingyun Yang, Linna Zhou, Cheryl M. J. Tan, Ming Lei, Hagan BayleyFisher Scientific). Cytotoxicity was measured by using a cell cytotoxicity assay kit (Colorimetric, ab112118, Abcam). Apoptosis was measured by using a caspase assay kit (Caspase-Glo 3/7 Assay, Promega). Standard

(9) Electrospun bioresorbable polymer membranes for coronary artery stentsFrontiers in Bioengineering and BiotechnologyAugust 21, 2024Maria A. Rezvova, Evgeny A. Ovcharenko, Kirill Yu Klyshnikov, Tatiana V. Glushkova, Alexander E. Kostyunin, Daria K. Shishkova, Vera G. Matveeva, Elena A. Velikanova, Amin R. Shabaev, Yulia A. Kudryavtsevaactivity was assessed using a colorimetric method with the Cell Cytotoxicity Assay Kit—Colorimetric (ab112118, Abcam, United States). The reagent, at a working concentration (1:5 with the nutrient medium), was

(10) Neurotoxic properties of the Zika virus envelope proteinExperimental NeurologySeptember 1, 2023Joseph P. Steiner, Muznabanu Bachani, Nasir Malik, Wenxue Li, Richa Tyagi, Kevon Sampson, Rachel P. M. Abrams, Youssef Kousa, Jamie Solis, Tory P. Johnson, et al.was performed using a water-soluble cellular reduction sensitive fluorescent dye (Abcam, catalog # ab112118) for an additional three hours. Absorption was measured on a Synergy H1 microplate reader (BioTek) with

(11) Rhodamine B-Conjugated Fluorescent Block Copolymer Micelles for Efficient Chlorambucil Delivery and Intracellular ImagingACS OmegaJune 27, 2023Bhagyashree Kulkarni, Somayah Qutub, Niveen M. Khashab, Nikos Hadjichristidis(Thermo Scientific) and 96-well plates (Corning Incorporated) were used. The cell cytotoxicity assay kit (ab112118) was purchased from Abcam. Measurements NMR measurements were performed at room temperature using

(12) Hydroxychloroquine Enhances Cytotoxic Properties of Extracellular Vesicles and Extracellular Vesicle–Mimetic Nanovesicles Loaded with ChemotherapeuticsPharmaceuticsFebruary 5, 2023Sergey Brezgin, Anastasiya Kostyusheva, Natalia Ponomareva, Ekaterina Bayurova, Alla Kondrashova, Anastasia Frolova, Olga Slatinskaya, Landysh Fatkhutdinova, Georgy Maksimov, Mikhail Zyuzin, et al.treated with EV-loaded DOX at a concentration of 100 nM. Afterwards, Cell Cytotoxicity Assay Kit Reagent (ab112118; Abcam) was added to cells according to the manufacturer’s instructions. Optical density was measured

(13) Modification of BRCA1-associated breast cancer risk by HMMR overexpressionNature CommunicationsDecember 1, 2022Francesca Mateo et al.screen, we first performed a dose response curve against HeLa cells using a cytotoxicity assay (Abcam ab112118). For each inhibitor, HeLa cells were incubated with eight titrated doses, including a DMSO control.

(14) Advantages of Fibrin Polymerization Method without the Use of Exogenous Thrombin for Vascular Tissue Engineering ApplicationsBiomedicinesMarch 28, 2022Vera G. Matveeva, Evgenia A. Senokosova, Viktoriia V. Sevostianova, Mariam Yu. Khanova, Tatiana V. Glushkova, Tatiana N. Akentieva, Larisa V. Antonova, Leonid S. BarbarashActivity Assay of ECFCs on the Fibrin Surface A single solution Cell Cytotoxicity Assay Kit-Colorimetric (ab112118, Abcam, Cambridge, UK) based on MTS (5-(3-carboxymethoxyphenyl)-2-(4,5-dimenthylthiazoly)-3-(4-sulfophenyl)

(15) Effect of glycerol addition time on the cryopreserved Korean native brindle cattle (Chikso) sperm qualityAnimal ReproductionMarch 11, 2022Lei Ma et al.Sunnyvale, CA, USA) to analyze it. Assessment of sperm viability We used the Abcam Cell Assay Kit (ab112118, Abcam, Cambridge, UK) to monitor cell viability. It uses a proprietary water-soluble dye that changes

(16) Safety, Stability, and Therapeutic Efficacy of Long-Circulating TQ-Incorporated Liposomes: Implication in the Treatment of Lung CancerPharmaceuticsJanuary 9, 2022Arif Khan et al.Cholesterol (Chol) were procured from Sigma-Aldrich (St. Louis, MO, USA). The Cell Cytotoxicity Assay kits (ab112118), Benzo [a] Pyrene (BaP) were, purchased from Abcam (Cambridge, USA). Dulbecco’s Modified Eagle Medium

(17) Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular InflammationInternational journal of molecular sciencesNovember 18, 2021Shishkova DK, Velikanova EA, Bogdanov LA, Sinitsky MY, Kostyunin AE, Tsepokina AV, Gruzdeva OV, Mironov AV, Mukhamadiyarov RA, Glushkova TV, et al.medium, and finally seeded into a 24-well plate. 4.8. Cytotoxicity Assays Following the addition of ab112118 (Abcam, Cambridge, U.K.) reagent, HCAECs and HITAECs cultured in 96-well plates (85–90% confluence)

(18) LPS Induces Opposing Memory-like Inflammatory Responses in Mouse Bone Marrow NeutrophilsInternational journal of molecular sciencesSeptember 10, 2021Lajqi T, Braun M, Kranig SA, Frommhold D, Pöschl J, Hudalla H.stressor, LPS, in schema endured in our study using the Cell Cytotoxicity Assay kit (colorimetric; #ab112118, Abcam, Cambridge, UK). Isolated cells (10,000 cell/well) were seeded into a 96-well plate and stimulated

(19) Assessment of cryopreserved sperm functions of Korean native brindled cattle (Chikso) from different region research centers of KoreaJournal of Animal Reproduction and BiotechnologyJune 30, 2021Lei Ma et al.the majority of the supernatant, 135 μL of DPBS and 15 μL of H33258 solution (10 g H33258/mL DPBS) (ab112118, Abcam, Cambridge, UK). This kit uses a pat- were added to the remaining sample. After incubation tra

(20) VEGF Mediates Retinal Müller Cell Viability and Neuroprotection through BDNF in DiabetesBiomoleculesMay 10, 2021Le YZ, Xu B, Chucair-Elliott AJ, Zhang H, Zhu M.staining. Cell viability assay was performed in 96-well plates using a cell cytotoxicity assay kit (ab112118) from Abcam (Cambridge, MA, USA), based on the manufacturer’s instruction. Briefly, 1.5 × 104 cells

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Cell Cytotoxicity Assay Kit (Colorimetric) from Abcam

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Cell Cytotoxicity Assay Kit (Colorimetric) from Abcam

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