Cytochrome c Release Assay Kit ab65311 from Abcam

Product Specs
ItemCytochrome c Release Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65311
Quantity100 Test
Sample TypeTissue, Adherent cells, Suspension cells
Assay Time3h 0m
Gene Namecycs
NCBI Gene AliasesCYC, HCS, THC4
Research AreaMetabolism
NCBI Full Gene Namecytochrome c, somatic

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Targeting ST18-mediated pathomechanism in pemphigus vulgaris through voltage-dependent anion channel inhibitionSkin Health and DiseaseMarch 31, 2026Sari Assaf, Ofer Sarig, Rawaa Ishtewy, Yazeed Zoabi, Yarden Feller, Kiril Malovitski, Janan Mohamad, Shir Bergson, Carmel Bilu, Varda Shoshan-Barmatz, et al.instructions, we isolated the mitochondrial fraction using the Cytochrome C Releasing Apoptosis Assay Kit (ab65311; Abcam). Dispase-based dissociation assay The assay was conducted as detailed elsewhere.13 The fragment

(2) Targeting ST18-mediated pathomechanism in pemphigus vulgaris through voltage-dependent anion channel inhibitionSkin Health and DiseaseJanuary 19, 2026Sari Assaf, Ofer Sarig, Rawaa Ishtewy, Yazeed Zoabi, Yarden Feller, Kiril Malovitski, Janan Mohamad, Shir Bergson, Carmel Bilu, Varda Shoshan-Barmatz, et al.instructions, we isolated the mitochondrial fraction using the Cytochrome C Releasing Apoptosis Assay Kit (ab65311; Abcam). Dispase-based dissociation assay The assay was conducted as detailed elsewhere.13 The fragment

(3) Branched-Chain α Keto-Acid Dehydrogenase Kinase-Mediated AKT Phosphorylation Promotes RCC Tumorigenesis and Drug ResistanceAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2025Tian Q, Wang J, Li Q, Liu Y, Chen Y, Feng J, Lei ZN, Patel H, Cai CY, Xu Y, et al.Release Cytochrome c release was detected using the Cytochrome c releasing apoptosis assay kit (Abcam, ab65311). Cells from each group were collected, centrifuged, and washed with 1×PBS before being resuspended

(4) Sevoflurane induces Ca2+ overload and EFHD1 upregulation, driving pyroptosis in SCLC cellsCytotechnologyJuly 17, 2025Bin Chen, Lu Wang, Qiuyue Liu, Tao Liu, Linghai Li, Teng Ma, Wei Liu, Jun Maassay kit (Nanjing Jiancheng Bioengineering Institute, E004-1-1), and Cytochrome c release assay kit (ab65311, Abcam). Cell lines and cell culture NCI-H446 cell line was purchased from Punosai Life Sciences,

(5) KS18, a Mcl-1 inhibitor, improves the effectiveness of bortezomib and overcomes resistance in refractory multiple myeloma by triggering intrinsic apoptosisFrontiers in PharmacologyOctober 1, 2024Omar S. Al-Odat, Weam Othman Elbezanti, Krishne Gowda, Sandeep K. Srivastava, Shantu G. Amin, Subash C. Jonnalagadda, Tulin Budak-Alpdogan, Manoj K. Pandeyincubation, the cytosolic fraction was extracted using the cytochrome C release assay kit (Abcam, #ab65311) according to the manufacturer’s instructions. Protein quantitation and western blotting were performed

(6) p17/C18-ceramide–mediated mitophagy is an endogenous neuroprotective response in preclinical and clinical brain injuryPNAS NexusFebruary 7, 2024Eda Karakaya, Natalia Oleinik, Jazlyn Edwards, Jensen Tomberlin, Randy Bent Barker, Burak Berber, Maria Ericsson, Habeeb Alsudani, Adviye Ergul, Semir Beyaz, et al.“mitochondrial fraction” (stored at −80 °C). Cytochrome c release assay Cytochrome c Release Assay Kit (ab65311, Abcam) protocol was followed. Briefly, after the mitochondria isolation, cytosolic and mitochondrial

(7) Salmonella enterica serovar Typhimurium remodels mitochondrial dynamics of macrophages via the T3SS effector SipA to promote intracellular proliferationGut microbesJanuary 1, 2024Liu X, Liu Y, Zhao X, Li X, Yao T, Liu R, Wang Q, Wang Q, Li D, Chen X, et al.replicates. Cytochrome c release Cytochrome c release was measured using a cytochrome c Releasing Apoptosis Assay kit (ab65311; Abcam) according to the manufacturer’s manual. Briefly, BMDMs were seeded in 75-cm2 culture bottles

(8) ER stress induces caspase‐2‐tBID‐GSDME‐dependent cell death in neurons lytically infected with herpes simplex virus type 2The EMBO JournalOctober 4, 2023Fanghui Ren, Ryo Narita, Ahmad S Rashidi, Stefanie Fruhwürth, Zongliang Gao, Rasmus O Bak, Martin K Thomsen, Georges MGM Verjans, Line S Reinert, Søren R Paludan(Abcam, ab39830), caspase 3 activity assay kit (Abcam, ab39401), cytochrome c release assay kit (Abcam, ab65311), annexin V conjugates for apoptosis detection (Invitrogen™, A13201), and CyQUANT™ LDH cytotoxicity

(9) Esophageal cancer stem cells reduce hypoxia-induced apoptosis by inhibiting the GRP78-perk-eIF2α-ATF4-CHOP pathway in vitroJournal of Gastrointestinal OncologyAugust 31, 2023Ruijiang Lin, Minjie Ma, Biao Han, Ya Zheng, Yuping Wang, Yongning Zhou(CA1020; Solarbio Co.); cycle detection kit (CA1510; Solarbio Co.); Cyt C releasing apoptosis assay kit (Ab65311; Abcam, Cambridge, MA, USA); radioimmunoprecipitation assay (RIPA) lysis buffer (P0013C; Beyotime Co.,

(10) Elephant TP53-RETROGENE 9 induces transcription-independent apoptosis at the mitochondriaCell death discoveryFebruary 16, 2023Preston AJ, Rogers A, Sharp M, Mitchell G, Toruno C, Barney BB, Donovan LN, Bly J, Kennington R, Payne E, et al.constructs. Cells were harvested after 48 h and processed using the Cytochrome C Release assay from Abcam (ab65311). The cytosolic and mitochondrial fractions were quantified by Bradford protein assay, then resuspended

(11) Selective Induction of Intrinsic Apoptosis in Retinoblastoma Cells by Novel Cationic Antimicrobial DodecapeptidesPharmaceuticsNovember 18, 2022Vishnu Suresh Babu, Atish Kizhakeyil, Gagan Dudeja, Shyam S. Chaurasia, Veluchami Amutha Barathi, Stephane Heymans, Navin Kumar Verma, Rajamani Lakshminarayanan, Arkasubhra Ghoshfollowed by mitochondrial extraction buffer using the cytochrome c releasing apoptosis assay kit (cat#ab65311, Abcam, Cambridge, UK) as per the manufacturer’s protocol. 20 µg of mitochondrial and cytosol protein

(12) Label-free chemical imaging of cytochrome P450 activity by Raman microscopyCommunications biologyAugust 22, 2022Li M, Nawa Y, Ishida S, Kanda Y, Fujita S, Fujita K.anti-CYP3A4 (1:2000, Sigma-Aldrich), rabbit anti-cyt b5 (1:1000, Abcam), mouse anti-cyt c (1:200, Abcam, ab65311), and rabbit anti-β-actin (1:1000; 4970 S; CST) primary antibody overnight. The following day, horseradish

(13) Lysosomes Dysfunction Causes Mitophagy Impairment in PBMCs of Sporadic ALS PatientsCellsApril 9, 2022Matteo Bordoni et al.(ab56788),mouse,1:500,Abcam MFN1 (ab57602),mouse,1:1000,Abcam OPA1 (ab157457),rabbit,1:1000,Abcam CYT C (ab65311),mouse,1:200,Abcam BCL-2 (ab59348),rabbit,1:200,Abcam Antibodies used in the WB analysis. We reported

(14) Platelet Mitochondria Transplantation Rescues Hypoxia/Reoxygenation-Induced Mitochondrial Dysfunction and Neuronal Cell Death Involving the FUNDC2/PIP3/Akt/FOXO3a AxisCell TransplantationJune 9, 2021Chun Shi, Han Guo, Xintong Liuleakage of cytochrome c (Cyto C) was analyzed by Western blotting with Cyto C-releasing assay kit (ab65311, Abcam, UK). Western blotting was conducted on whole-cell, cytoplasmic and mitochondrial lysates with

(15) γ‑irradiated prednisolone promotes apoptosis of liver cancer cells via activation of intrinsic apoptosis signaling pathwayMolecular Medicine ReportsApril 7, 2021Fatuma Jumapili Ramadhani, Seong Hee Kang, Remigius Ambrose Kawala, Byung Yeoup Chung, Hyoung-Woo Bai, Bo Sun Kang(BCA) reagent was obtained from Thermo Fisher Scientific, Inc. The cytochrome c release kit (cat. no. ab65311) was from Abcam. Anti-GAPDH, anti-cleaved caspase 3, anti-cleaved caspase 9, anti-caspase 9, anti-cleaved

(16) Collapsin response mediator protein 4 enhances the radiosensitivity of colon cancer cells through calcium‑mediated cell signalingOncology reportsApril 1, 2021Park SY, Kim JT, Park ES, Hwang YS, Yoon HR, Baek KE, Jung H, Yoon SR, Kim BY, Cho HJ, et al.flow cytometry. Measurement of cytochrome c release The cytochrome c- releasing apoptosis assay kit (Abcam; ab65311) was used for detecting cytochrome c translocation from mitochondria into the cytosol. After irradiated

(17) Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitmentClinical oral investigationsApril 1, 2021Ruiz-Heiland G, Yong JW, von Bremen J, Ruf S.Thermo-Fisher) dilution 1:1000; phospho-STAT3 Ser727 (OPA1-03007, ThermoFisher) dilution 1:500; Cytochrome C (ab65311, Abcam) dilution 1:1000; cPLA2α (orb100010, BIOZOL) dilution 1:1000; cPLA2β (ab198898, Abcam) dilution

(18) Brain immune cells undergo cGAS/STING-dependent apoptosis during herpes simplex virus type 1 infection to limit type I IFN productionJournal of Clinical InvestigationJanuary 4, 2021Line S. Reinert et al.cytosolic fractions of stimulated cells were separated using 14 the Cytochrome C Release Assay Kit (Abcam, ab65311) according to the manufacturer’s protocol, and 30 μg of the cytosolic and mitochondrial fractions was

(19) Amino acid depletion triggered by ʟ-asparaginase sensitizes MM cells to carfilzomib by inducing mitochondria ROS-mediated cell deathBlood AdvancesSeptember 22, 2020Debora Soncini et al.from mitochondria into cytosol during apoptosis was assayed using a Cytochrome c Release Assay Kit (ab65311; Abcam), according to the manufacturer’s instructions. Cell viability and apoptosis assay The viability

(20) Adrenergic-Independent Signaling via CHRNA2 Regulates Beige Fat ActivationDevelopmental CellJuly 1, 2020Heejin Jun et al.Assays,Critical Commercial Assays,Critical Commercial Assays Cytochrome c Release Assay Kit,Abcam,Cat# ab65311 Citrate Synthase Activity Assay Kit,Cayman chemical,Cat# 701040 L-Lactate Assay Kit,Cayman chemical,Cat#

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Cytochrome c Release Assay Kit from Abcam

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Cytochrome c Release Assay Kit from Abcam

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