JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) ab112133 from Abcam

Product Specs
ItemJC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112133
Quantity100 Test
MethodFlow Cyt
Sample TypeAdherent cells, Suspension cells
Assay Time0h 20m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Apoptotic Events in Type-I Glanzmann Thrombasthenia PlateletsInternational Journal of Hematology-Oncology and Stem Cell ResearchOctober 20, 2025Ehsan Shahverdi, Corinna Petz, Turadj Hemmati, Alireza Farsinezhad, Ali Heidari, Mohammad Faranoushpotential-sensitive dye JC-10, enhanced 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolcarbocyanine iodide (AB112133, Abcam, Cambridge Science Park, Cambridge, England), was used to assess depolarization of the mitochondrial

(2) PKCδ Regulates DNA Damage and Cell Death through a SIRT6/Nrf2-dependent Antioxidant ResponseMolecular Cancer ResearchSeptember 4, 2025Trisiani Affandi, Angela M. Ohm, Jordan T. Speidel, M. Cecilia Caino, Dillon P. Boulton, Mary E. Reylandmin. To measure mitochondrial membrane potential (MMP), cells were incubated with JC-10 dye (1X, Cat # ab112133, Abcam) for 30 min, collected using trypsin, washed, and resuspended in PBS to produce single cell suspension.

(3) PKCδ regulates DNA damage and cell death through a SIRT6/Nrf2-dependent antioxidant responseMol Cancer ResSeptember 4, 2025Trisiani Affandi, Angela M. Ohm, Jordan T. Speidel, M. Cecilia Caino, Dillon P. Boulton, Mary E. Reylandmin. To measure mitochondrial membrane potential (MMP), cells were incubated with JC-10 dye (1X, Cat # ab112133, Abcam) for 30 min, collected using trypsin, washed, and resuspended in PBS to produce single cell suspension.

(4) The cryoprotective effects of celastrol nanoemulsion on post-thawed attributes and fertilizing ability of cryopreserved buffalo semenVet Res CommunJune 5, 2025Wael A. Khalil, Salwa A. Elkhamy, Mohamed M. Hegazy, Mahmoud A. E. Hassan, Sameh A. Abdelnour, Mostafa A. El-Harairywere washed with PBS and analyzed for mitochondrial membrane potential (MMP) using a JC-10 assay kit (ab112133; Abcam Company), following the manufacturer’s instructions. Approximately 1 × 106 sperm cells per milliliter

(5) Oxidized Cell-Free Hemoglobin Induces Mitochondrial Dysfunction by Activation of the Mitochondrial Permeability Transition Pore in the Pulmonary MicrovasculatureMicrocirculationMay 20, 2025Kyle J. Riedmann, Jamie E. Meegan, Aqeela Afzal, Yatzil Cervantes-Cruz, Sarah Obeidalla, Avery M. Bogart, Lorraine B. Ware, Ciara M. Shaver, Julie A. Bastarachewere treated and harvested as described above before being resuspended in 1× JC10 in assay buffer (ab112133; Abcam, Cambridge, MA) and incubated at 37°C for 30 min prior to analysis on an LSRFortessa using the

(6) Cardamonin suppresses mTORC1/SREBP1 through reducing Raptor and inhibits de novo lipogenesis in ovarian cancerPLoS OneMay 2, 2025Peiguang Niu, Danyun Li, Huajiao Chen, Yanting Zhu, Jintuo Zhou, Jinhua Zhang, Ying Liupotential (MMP) measurement MMP was evaluated using the JC-10 Mitochondrial Membrane Potential Assay Kit (ab112133; Abcam). Treat with cardamonin for 48 h, cells were collected and washed with cold PBS twice. 5 × 105

(7) Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1iScienceAugust 18, 2023Tong L, Rao J, Yang C, Xu J, Lu Y, Zhang Y, Cang X, Xie S, Mao J, Jiang P."MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging",Invitrogen,Cat# M36008 JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry),Abcam,Cat#ab112133 CellTiter-Glo Luminescent Cell Viability Assay,Promega,Cat#G7571 Reverse transcription kit,Takara,Cat#6110A Cell

(8) Optimized allotopic expression of mitochondrial ND6 transgene restored complex I and apoptosis deficiencies caused by LHON-linked ND6 14484T > C mutationJournal of biomedical scienceAugust 3, 2023Wang J, Ji Y, Ai C, Chen JR, Gan D, Zhang J, Mo JQ, Guan MX.potential Mitochondrial membrane potential was measured with a JC-10 Mitochondrial Membrane Potential Assay Kit (ab112133, Abcam, Cambridge, United Kingdom), according to the manufacturer’s general recommendations, with some

(9) Effects of the PARP Inhibitor Olaparib on the Response of Human Peripheral Blood Leukocytes to Bacterial Challenge or Oxidative StressBiomoleculesJune 4, 2022Santos SS, Brunialti MKC, Rodrigues LOCP, Liberatore AMA, Koh IHJ, Martins V, Soriano FG, Szabo C, Salomão R.Mitochondrial Membrane Potential (MMP) Measurement MMP was measured by flow cytometry using a JC-10 Assay Kit (ab112133; Abcam). PBMCs (2.5 × 106/mL)were incubated in nonadherent tubes in a 5% CO2 incubator at 37 °C with

(10) Inhibition of nuclear factor (erythroid-derived 2)-like 2 promotes hepatic progenitor cell activation and differentiationnpj Regenerative MedicineDecember 1, 2021Francesco Bellanti et al.(BioLegend, 424101) for 15 min, at RT. Mitochondrial membrane potential was analyzed using JC-10 (Abcam, ab112133) as a probe. In all, 5.0 × 105 cells were incubated with JC-10 for 15 min at 37 °C in the dark before

(11) Cisplatin Activates the Growth Inhibitory Signaling Pathways by Enhancing the Production of Reactive Oxygen Species in Non-small Cell Lung Cancer Carrying an EGFR Exon 19 DeletionCancer Genomics - ProteomicsMarch 12, 2021MD MOHIUDDIN, KAZUO KASAHARAevaluation. The MMP was evaluated using the JC-10 Mitochondrial Membrane Potential Assay Kit-Flow Cytometry (ab112133; Abcam). Cells were seeded in 75-cm2 flasks (Falcon, USA), treated as indicated in the results, trypsinized,

(12) Characterization of Pathogenesis and Inflammatory Responses to Experimental Parechovirus EncephalitisFrontiers in immunologyJanuary 1, 2021Jan MW, Su HL, Chang TH, Tsai KJ.with PeV-A3 at MOI = 1 for 24 h. After infection, cells were harvested for JC-10 analysis (Abcam, #ab112133, Cambridge, England). Cells were suspended in 500 µl of JC-10 solution and incubated at 37°C for 45

(13) Targeting TRAP1 as a downstream effector of BRAF cytoprotective pathway: a novel strategy for human BRAF-driven colorectal carcinomaOncotargetSeptember 8, 2015Condelli V, Maddalena F, Sisinni L, Lettini G, Matassa DS, Piscazzi A, Palladino G, Amoroso MR, Esposito F, Landriscina M.potential was detected by using JC-10 Mitochondrial Membrane Potential Assay Kit – Flow Cytometry (Abcam, ab112133). Cells were seeded into 6-well plates, treated as indicated in the Results, trypsinized, washed with

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JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) from Abcam

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JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) from Abcam

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