JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) ab112134 from Abcam

Product Specs
ItemJC-10 Mitochondrial Membrane Potential Assay Kit (Microplate)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112134
MethodFuncS
Quantity5 96TESTS
Sample TypeAdherent cells, Suspension cells
Assay Time1h 0m
ApplicationsCellular Assay
Detection TargetMitochondrial Membrane Potential

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Mesenchymal stromal cells secretome restores bioenergetic and redox homeostasis in human proximal tubule cells after ischemic injuryStem Cell Res TherDecember 10, 2023João Faria, Sandra Calcat-I-Cervera, Renata Skovronova, Bonnie C. Broeksma, Alinda J. Berends, Esther A. Zaal, Benedetta Bussolati, Timothy O'Brien, Silvia M. Mihăilă, Rosalinde Masereeuwpotential (Δψ) The integrity of mitochondrial membrane potential (Δψ) was measured using JC-10 (Abcam, ab112134) [39]. ciPTECs were rinsed once with HBSS and incubated with 50 μl of JC-10 loading solution for 30

(2) Development of an iron overload HepG2 cell model using ferrous ammonium citrateSci RepDecember 8, 2023Usama Abbasi, Srinivas Abbina, Arshdeep Gill, Jayachandran N. KizhakkedathuDCFDA/H2DCFDA ROS generation kit (ab113851) and the JC-10 mitochondrial membrane potential assay kit (ab112134) were purchased from Abcam, while the MTT assay kit was purchased from ATCC (20-1010K). Cell culture HepG2 cells

(3) The DNA damage response in advanced ovarian cancer: functional analysis combined with machine learning identifies signatures that correlate with chemotherapy sensitivity and patient outcomeBritish journal of cancerMay 1, 2023Walker TDJ, Faraahi ZF, Price MJ, Hawarden A, Waddell CA, Russell B, Jones DM, McCormick A, Gavrielides N, Tyagi S, et al.1.7. Intra-explant mitochondrial membrane function Explant mitochondrial membrane potential was assayed by JC-10 (ab112134, Abcam) upon live-cell cultures. Cells were seeded at 25,000 cells per well and incubated with a 5-point

(4) IL-17A promotes lung fibrosis through impairing mitochondrial homeostasis in type II alveolar epithelial cellsJournal of cellular and molecular medicineNovember 1, 2022Xiao H, Peng L, Jiang D, Liu Y, Zhu L, Li Z, Geng J, Xie B, Huang X, Wang J, et al.in relative MMP were measured using the JC‐10 Mitochondrial Membrane Potential Assay Kit‐Microplate (ab112134, Abcam, Cambridge, UK) according to the manufacturer's instructions. Briefly, 5 × 104 cells/well were

(5) Complement membrane attack complex is an immunometabolic regulator of NLRP3 activation and IL-18 secretion in human macrophagesFrontiers in ImmunologySeptember 27, 2022Gisela Jimenez-Duran, Joseph Kozole, Rachel Peltier-Heap, Eleanor R. Dickinson, Christopher R. Kwiatkowski, Francesca Zappacosta, Roland S. Annan, Nicholas W. Galwey, Eva-Maria Nichols, Louise K. Modis, et al.Assays,Critical Commercial Assays JC-10 Mitochondrial Membrane Potential Assay Kit – Microplate,Abcam,ab112134 Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit,Invitrogen,A22188 Cell-TiterGlo kit,Promega,G7571 Calcein

(6) ZnT8 loss-of-function accelerates functional maturation of hESC-derived β cells and resists metabolic stress in diabetesNature communicationsJuly 16, 2022Ma Q, Xiao Y, Xu W, Wang M, Li S, Yang Z, Xu M, Zhang T, Zhang ZN, Hu R, et al.seeded onto 3.5 cm Matrigel-coated glass-bottom petri dishes for 24 h and loaded with JC-10 (Abcam, #ab112134, 10 mg ml−1) for 1 h. During the period of JC-10 staining, several dishes of HUES8 SC-β cells were treated

(7) Growth Differentiation Factor 15 Protects SH-SY5Y Cells From Rotenone-Induced Toxicity by Suppressing Mitochondrial ApoptosisFrontiers in Aging NeuroscienceJune 2, 2022Peizheng Li, Hongbo Lv, Bohan Zhang, Ruonan Duan, Xiufang Zhang, Pengfei Lin, Chengyuan Song, Yiming LiuPotential Measurements To evaluate the mitochondrial membrane potential (MMP), we used a JC-10 assay kit (ab112134, Abcam). Following the commercial protocols, cells were cultured in a 96-well black plate with a transparent

(8) A Synthetic Small RNA Homologous to the D-Loop Transcript of mtDNA Enhances Mitochondrial BioenergeticsFrontiers in PhysiologyApril 6, 2022Theodore L. Mathuram et al.Mitochondrial membrane potential was measured using a JC-10 mitochondrial membrane potential assay kit (Abcam, cat. #ab112134) according to the manufacturer’s instructions. The results were read by a BioTek Synergy HT spectrophotometer. Preparation

(9) Cannabinol inhibits oxytosis/ferroptosis by directly targeting mitochondria independently of cannabinoid receptorsFree Radical Biology and MedicineFebruary 1, 2022Zhibin Liang, David Soriano-Castell, Devin Kepchia, Brendan M. Duggan, Antonio Currais, David Schubert, Pamela Maherfrom Thermo Fisher Scientific (Waltham, MA). JC-10 Mitochondrial Membrane Potential Assay Kit (Cat# ab112134) was from Abcam (Burlingame, CA). Criterion XT Bis-Tris Protein Gradient Gel (Ca# 3450125), XT MOPS Running Buffer

(10) Chemically induced senescence in human stem cell-derived neurons promotes phenotypic presentation of neurodegenerationAging cellJanuary 1, 2022Fathi A, Mathivanan S, Kong L, Petersen AJ, Harder CRK, Block J, Miller JM, Bhattacharyya A, Wang D, Zhang SC.mode. Mitochondrial membrane potential assay was performed using JC‐10 mitochondrial membrane potential assay kit (ab112134, Abcam). H9‐derived Cortical neuron progenitors and 298G and 298S hiPSC‐derived MN progenitors were

(11) Alternative mitochondrial quality control mediated by extracellular releaseAutophagyOctober 1, 2021Choong CJ, Okuno T, Ikenaka K, Baba K, Hayakawa H, Koike M, Yokota M, Doi J, Kakuda K, Takeuchi T, et al.assay Mitochondrial membrane potential assay was performed using the cationic dye JC10, and an assay kit (Abcam, ab112134), following the manufacturer’s protocol. Confocal images of JC10 stained cells were acquired on same

(12) Alcohol-abuse drug disulfiram targets pediatric glioma via MLL degradationCell death & diseaseAugust 11, 2021Meier S, Cantilena S, Niklison Chirou MV, Anderson J, Hargrave D, Salomoni P, de Boer J, Michod D.reader. Mitochondrial membrane potential assay JC-10 Mitochondrial Membrane Potential Assay (Abcam, ab112134) was used according to the manufacturer’s protocol. Luminescence was measured using a TECAN infinite

(13) Hypoxia-induced mitochondrial abnormalities in cells of the placentaPloS oneJanuary 1, 2021Vangrieken P, Al-Nasiry S, Bast A, Leermakers PA, Tulen CBM, Janssen GMJ, Kaminski I, Geomini I, Lemmens T, Schiffers PMH, et al.potential Mitochondrial membrane potential was assessed as instructed by the manufacturer’s protocol (JC-10 assay kit, ab112134, Abcam, Cambridge, Massachusetts, USA) according to the manufacturer’s protocol. Briefly, BeWo cells

(14) Retrograde signaling by a mtDNA-encoded non-coding RNA preserves mitochondrial bioenergeticsCommunications biologyOctober 30, 2020Blumental-Perry A, Jobava R, Bederman I, Degar AJ, Kenche H, Guan BJ, Pandit K, Perry NA, Molyneaux ND, Wu J, et al.assays Mitochondrial membrane potential was measured using the JC-10 Mitochondrial Membrane Potential Assay Kit (ab112134, Abcam, USA) according to the manufacturer’s instructions. Results were read by a Biotek Synergy HT

(15) Dietary magnesium supplementation improves lifespan in a mouse model of progeriaEMBO Molecular MedicineOctober 7, 2020Ricardo Villa‐Bellostapotential (ΔΨm) measurement The ΔΨm was assessed by plate reader using a fluorescent probe (JC‐10; ab112134, Abcam) by following the manufacturer's instruction. When mitochondria are polarized electrically, JC‐10

(16) 12(S)-HETE mediates diabetes-induced endothelial dysfunction by activating intracellular endothelial cell TRPV1The Journal of clinical investigationSeptember 1, 2020Otto M, Bucher C, Liu W, Müller M, Schmidt T, Kardell M, Driessen MN, Rossaint J, Gross ER, Wagner NM.function. Mitochondrial membrane potential was assessed using the JC-10 mitochondrial membrane potential assay kit (ab112134, Abcam) and a BD Accuri flow cytometer or by life cell imaging after loading of cells with 150 nM tetramethylrhodamine

(17) Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNA^Thr GeneJournal of diabetes researchJanuary 1, 2020Li K, Wu L, Liu J, Lin W, Qi Q, Zhao T.Mitochondrial Membrane Potential Measurement A JC-10 Mitochondrial Membrane Potential Microplate Assay Kit (Abcam, ab112134) was utilized based on the provided protocols, as in the previous studies, as a means of assessing the

(18) 1-Trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) Urea, a Selective and Potent Dual Inhibitor of Soluble Epoxide Hydrolase and p38 Kinase Intervenes in Alzheimer's Signaling in Human Nerve CellsACS chemical neuroscienceSeptember 18, 2019Liang Z, Zhang B, Xu M, Morisseau C, Hwang SH, Hammock BD, Li QX.(Cat# 5000002) was from Bio-Rad (Hercules, CA). JC-10 Mitochondrial Membrane Potential Assay Kit (Cat# ab112134) was from Abcam (Burlingame, CA). TransAM NF-κB Family ELISA Assay Kit (Cat# 43296) was from Active

(19) Molecular mechanism of cardol, isolated from Trigona incisa stingless bee propolis, induced apoptosis in the SW620 human colorectal cancer cell lineBMC Pharmacology and ToxicologyDecember 1, 2017Paula Mariana Kustiawan, Kriengsak Lirdprapamongkol, Tanapat Palaga, Songchan Puthong, Preecha Phuwapraisirisan, Jisnuson Svasti, Chanpen Chanchaomitochondrial membrane polarization was measured using a JC-10 mitochondrial membrane potential assay kit (catalog # ab112134, Abcam, Cambridge, MA) according to the manufacturer’s instructions. SW620 cells were seeded (8 x 104

(20) Aminoglycoside stress together with the 12S rRNA 1494C>T mutation leads to mitophagyPloS oneJanuary 1, 2014Yu J, Zheng J, Zhao X, Liu J, Mao Z, Ling Y, Chen D, Chen C, Hui L, Cui L, et al.for 24 hours. Then, following the manufacturer’s instructions, treatment with the JC-10 kit (Abcam, ab112134) was performed as follows: 50 µl of JC-10 was added per well, followed by incubation for 1 hour before

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JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) from Abcam

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JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) from Abcam

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