JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) ab112134 from Abcam

Product Specs
ItemJC-10 Mitochondrial Membrane Potential Assay Kit (Microplate)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112134
MethodFuncS
Quantity5 x 96 Tests
Sample TypeAdherent cells, Suspension cells
Assay Time1h 0m

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Stress-Activated Pathways Mediate PFAS Effects on Human Placental Syncytiotrophoblast CellsToxicological SciencesJune 5, 2026Keisuke Kozai, Kaela M Varbergmeasured mitochondrial membrane potential using the JC-10 Mitochondrial Membrane Potential Assay Kit (ab112134, Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions. Fluorescence intensities

(2) Ambigols Uncouple Oxidative Phosphorylation In VitroACS Chem BiolApril 17, 2026Valerie I. C. Rebhahn, Clemens A. Wolf, Timo H. J. Niedermeyertransparent bottom, and incubated with JC-10 from the JC-10 Membrane Potential Assay Kit for microplates (no. AB112134, Abcam, Cambridge, UK) for 1 h at the respective temperature. The kit was applied following the manufacturer’s

(3) Synergistic Anticancer Effects of Lenvatinib Combined with N-butylidenephthalide in Human Colorectal Cancer CellsInternational Journal of Medical SciencesMarch 4, 2026Cheng-Chan Yu, Sung-Ying Huang, Shu-Fang Chang, Yu-Hung Kuo, Kuan-Fu Liao, Sheng-Chun Chiupotential measurement Mitochondrial membrane potential (MMP) was assessed using a JC-10 assay kit (Abcam, ab112134). CRC cells were seeded in 24-well plates overnight, treated with the indicated drugs for 24 h, and

(4) Synergistic Neuroprotection in Parkinson’s Disease via Photobiomodulation and Liposomal Rosmarinic Acid DeliveryACS Biomaterials Science & EngineeringFebruary 9, 2026Ting-Yi Su, Chen-Ya Wang, Wen-Tse Huang, Ming-Yang Chang, Ming-Hsien Chan, Ru-Shi Liuconditions by fluorescence detection. The procedure follows the protocol provided by the supplier (Abcam, ab112134, USA). After the treatment of cells, diluted JC-1 was added and incubated for 30 min. Afterward, buffer

(5) A high-throughput screening platform to facilitate treatment development in Rett syndromeFrontiers in neurologyJanuary 1, 2026Yin Y, Wang A, Dong Q, Zhang Z, Bu Q, Wang R, Chang Q.in Supplementary Table S4. JC-10 assay MMP was determined by the JC-10 Assay Kit (Abcam, Catalog#ab112134), following the manufacturer’s protocol. Astrocytes were imaged using the PerkinElmer Operetta® or a

(6) RAS/PI3K pathway mutations sensitise epithelial ovarian cancer cells to a PARP/NAMPT inhibitor combinationCommun BiolDecember 19, 2025Michael Gruet, Yitao Xu, Lyutong An, Yurui Ma, Cristina Balcells, Katie Tyson, Laila C. Evangelista, Sarah Spear, Yuewei Xu, Flora McKinney, et al.drug treatment, mitochondrial membrane potential (MMP) was measured using the JC-10 assay kit (Abcam, ab112134) according to the manufacturer’s instructions. In brief, 50 μl of assay buffer A with JC-10 dye was

(7) Reprogramming oncogenic mitochondria in pancreatic adenocarcinoma through BRD4 inhibition leads to programmed cell deathThe Journal of Pharmacology and Experimental TherapeuticsNovember 1, 2025Chun Cai, Michael W. Spinrad, Lauren C. Gattie, Rui Wang, Mohammad Amir Afjal, Jun Yang, Nour Yadak, David Shibata, Wei Li, Amandeep Bajwa, et al.apoptosis induced by BMS-986158, we used the JC-10 mitochondrial membrane potential assay kit (Abcam, ab112134). A total of 5 × 104 cells were seeded into a black, clear-bottomed 96-well plate and treated with either

(8) Gene and drug-mediated SERCA2a activation restores cardiac function and metabolic balance in diabetic miceBiomedicine & PharmacotherapyNovember 1, 2025Soojeong Kang, Abdul Majid, Nadjib Hammoudi, Djamel Lebechemitochondrial membrane potential and cell death. For mitochondrial membrane potential assessment (Abcam; ab112134), the fluorescent intensity of JC-10 was measured with excitation/emission wavelengths of 490/525 nm

(9) Neuroprotective Effects of Asiatic Acid on Autophagy and Mitochondrial Integrity in a Parkinson’s Disease Cellular ModelJ Exp PharmacolOctober 1, 2025Athinan Prommahom, Tatcha Balit, Sunisa Somkana, Satjapot Manprasong, Chonlakorn Panyasuppakun, Atipha Kijkraikul, Preawanit Thawornrungroaj, Pitchaya Thawornrungroaj, Permphan Dharmasaroja, Thanasup Gonmanee, et al.fluorescence microscope (ZEISS). Measurement of Δψm The ΔΨm was quantified using the JC-10 ΔΨm assay kit (ab112134, Abcam) and a microplate reader. SH-SY5Y cells were distributed into 96-well plates at 20,000 cells/well.

(10) Neuroprotective Effects of Asiatic Acid on Autophagy and Mitochondrial Integrity in a Parkinson's Disease Cellular ModelJ Exp PharmacolOctober 1, 2025Athinan Prommahom, Tatcha Balit, Sunisa Somkana, Satjapot Manprasong, Chonlakorn Panyasuppakun, Atipha Kijkraikul, Preawanit Thawornrungroaj, Pitchaya Thawornrungroaj, Permphan Dharmasaroja, Thanasup Gonmanee, et al.fluorescence microscope (ZEISS). Measurement of Δψm The ΔΨm was quantified using the JC-10 ΔΨm assay kit (ab112134, Abcam) and a microplate reader. SH-SY5Y cells were distributed into 96-well plates at 20,000 cells/well.

(11) Effect of human adipose stem cell-derived mitochondrial transplantation on the activity of chronically injured anterior cruciate ligament cellsBone Joint ResSeptember 19, 2025Hon Lok Lo, Yao-Hui Huang, Yu-Chuan Lin, Wen-Wei Li, Shun Cheng Wu, Pei-Hsi Chou, Cheng-Chang Lumitochondrial membrane potential was measured using JC-10 Mitochondrial Membrane Potential Assay Kit (cat no. ab112134; Abcam, UK), following the manufacturer’s protocol to investigate changes in mitochondrial membrane

(12) Effect of human adipose stem cell-derived mitochondrial transplantation on the activity of chronically injured anterior cruciate ligament cellsBone & Joint ResearchSeptember 19, 2025Hon Lok Lo, Yao-Hui Huang, Yu-Chuan Lin, Wen-Wei Li, Shun Cheng Wu, Pei-Hsi Chou, Cheng-Chang Lumitochondrial membrane potential was measured using JC-10 Mitochondrial Membrane Potential Assay Kit (cat no. ab112134; Abcam, UK), following the manufacturer’s protocol to investigate changes in mitochondrial membrane

(13) Aetokthonotoxin, the Causative Agent of Vacuolar Myelinopathy, Uncouples Oxidative Phosphorylation due to Protonophore ActivityChemical Research in ToxicologySeptember 15, 2025Valerie I. C. Rebhahn, Mohamad Saoud, Mathias Winterhalter, Franziska Schanbacher, Maximilian Jobst, Rebeca Ruiz, Alexander Sonntag, Johannes Kollatz, Rieke Sprengel, Stephen F. Donovan, et al.and (dn-AETX) to the cells. The JC-10 Mitochondrial Membrane Potential Assay Kit for microplates (no. AB112134, Abcam, Cambridge, UK) was used following the manufacturer’s instructions. Fluorescence of JC-10 was

(14) Imeglimin improves hyperglycemia and hypoglycemia‐induced cell death and mitochondrial dysfunction in immortalized adult mouse Schwann IMS32 cellsJournal of Diabetes InvestigationSeptember 1, 2025Ayako Kato, Wataru Nihei, Hideji Yako, Yasuaki Tatsumi, Tatsuhito Himeno, Masaki Kondo, Yoshiro Kato, Jiro Nakamura, Hideki Kamiya, Kazunori Sango, et al.potential Mitochondrial membrane potential was assessed using a JC‐10 Mitochondrial Membrane Potential Assay Kit (ab112134: Abcam, Cambridge, MA, USA) according to the manufacturer's instructions. Cells were treated with JC‐10

(15) Pyrimethamine Inhibits Human Ovarian Cancer by Triggering Lethal Mitophagy via Activating the p38/JNK/ERK PathwayOncology ResearchAugust 28, 2025Lingjuan Linghu, Hongying Zhou, Gang Zheng, Tao YiImageJ. 2.4.4 Mitochondrial Polarization Assay The mitochondrial polarization was assessed utilizing JC-10 (ab112134, Abcam), a dye that selectively targets mitochondria and exhibits a reversible color transition from

(16) Mitochondrial tRNAGlu 14687A>G May Be A Novel Mutation for Type 2 Diabetes MellitusJournal of Clinical Laboratory AnalysisJune 12, 2025Xiaojuan Rao, Liran Xie, Shuangwei Shi, Yifan Fangmicroplate reader (Syneregy H1, BioTek). 2.9 MMP Measurement The JC‐10 MMP microplate assay kit (Abcam, ab112134) was used according to previously described protocols [33]. Approximately 2 × 106 cells of each cybrid

(17) PPARα-mediated lipid metabolism reprogramming supports anti-EGFR therapy resistance in head and neck squamous cell carcinomaNat CommunFebruary 1, 2025Valentin Van den Bossche, Julie Vignau, Engy Vigneron, Isabella Rizzi, Hannah Zaryouh, An Wouters, Jérôme Ambroise, Steven Van Laere, Simon Beyaert, Raphaël Helaers, et al.mitochondrial membrane potential (Δψm) was measured using the JC-10 Mitochondrial Membrane Potential Assay Kit (Abcam, #ab112134), according to manufacturer’s recommendations. Cancer cells (2.104 cells/well) were seeded in 96-well

(18) Vitamin C alleviates hyperglycemic stress in retinal pigment epithelial cellsMolecular Biology ReportsDecember 1, 2024Hamid Alahmari, Chia-Chun Liu, Elizabeth Rubin, Venice Y. Lin, Paul Rodriguez, Kun-Che Changincubated with low glucose or high glucose treatment for 5h. 50 μl of JC-10 dye loading solution (Abcam, ab112134) was added into each well (containing 100 μl medium) for 1h at 37 °C. Then, 50 μl assay buffer B was

(19) Astrocyte Mitochondria Are a Sensitive Target of PCB52 and its Human-Relevant MetabolitesACS Chemical NeuroscienceAugust 7, 2024Neha Paranjape, Stefan Strack, Hans-Joachim Lehmler, Jonathan A. Doornthe manufacturer’s instructions for the Abcam mitochondrial membrane potential assay kit (Cat. no. ab112134) were followed. Fluorescence intensity was measured using a BioTek Synergy HTX multimode plate reader

(20) Metformin in combination with chemotherapy increases apoptosis in gastric cancer cells and counteracts senescence induced by chemotherapyOncology LettersJuly 25, 2024Katia Carolina Vázquez‑Ibarra, Josefina Yoaly Sánchez López, Tomás Daniel Pineda Razo, José Roberto Cruz Lozano, Brenda Guadalupe Ortiz‑Tamayo, Luis Arturo Palafox‑Mariscal, Rosa María González Arreola, Juan Ramón González‑García, Pablo Cesar Ortiz-Lazarenoshown). Determination of ΔΨm in GC cells The loss of ΔΨm was determined using JC-10 reagent (cat. no. ab112134; Abcam). NCI-N87 cells (4×104 cells/well) were seeded in 96-well plates with black wells and clear bottoms

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JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) from Abcam

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JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) from Abcam

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