CytoScan™ LDH Cytotoxicity Assay 786-210 from G Biosciences

Product Specs
ItemCytoScan™ LDH Cytotoxicity Assay
CompanyG Biosciences
Price $300.00Supplier Page View Company Product Page
Catalog Number786-210
Quantity1000 Assays
MethodColorimetric / Chromogenic
ApplicationsFor the detection of cell toxicity, death, viability or proliferation For cell free supernatants from cells in culture (adherent or suspension)

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G Biosciences

9800 Page Avenue

St. Louis, Missouri 63132

United States

Phone: +800-628-7730
Fax: 314-991-1504

Company Profile

Website: http://www.gbiosciences.com

(1) Effects of Hydroxysafflor Yellow A (HSYA) on UVA-Induced Damage in HaCaT KeratinocytesInternational Journal of Molecular SciencesJuly 10, 2024Szu-Chieh Yu, Wan-Chun Chiu, Pei Yu Loe, Yi-Wen ChienAssay The LDH assay followed the procedures described in the LDH cytotoxicity assay kit (G-Biosciences cat. 786-210, 786-324). The assay plate was incubated for 24 h in a humidified chamber at 37 °C and 5% CO2. At 45

(2) The adiponectin‐derived peptide ALY688 protects against the development of metabolic dysfunction‐associated steatohepatitisClinical and Translational ScienceJune 7, 2024Zhe Huang, Hye Kyoung Sung, Xingqun Yan, Shiyu He, Leigang Jin, Qin Wang, Xuerui Wu, Henry H. Hsu, Angelica Pignalosa, Kathryn Crawford, et al.Aldrich) and lactate dehydrogenase (LDH) release according to the manufacturer's instructions (Cat. No. 786‐210, G‐Biosciences, St. Louis, MO, USA). For evaluation of adiponectin‐like signaling, human induced pluripotent

(3) Cinnamaldehyde inhibits the NLRP3 inflammasome by preserving mitochondrial integrity and augmenting autophagy in Shigella sonnei-infected macrophagesJournal of InflammationJune 5, 2024Kuo-Feng Hua, Yu-Bei Lin, Hsiao-Wen Chiu, Wei-Ting Wong, Shuk-Man Ka, Chun-Hsien Wu, Wen-Yu Lin, Chien-Chun Wang, Chung-Hua Hsu, Hsien-Ta Hsu, et al.(NBP2-33422) were purchased from Novus Biologicals (Littleton, CO). The CytoScan LDH Cytotoxicity Assay kit (786 − 210) was purchased from G-Bioscience (St. Louis, MO). The pNiFty2-SEAP plasmid (pnifty2-seap), QUANTI-Blue

(4) Antrodia cinnamomea May Interfere with the Interaction Between ACE2 and SARS-CoV-2 Spike Protein in vitro and Reduces Lung Inflammation in a Hamster Model of COVID-19Journal of Inflammation ResearchOctober 26, 2023Lan-Hui Li, Hsiao-Wen Chiu, Wei-Ting Wong, Ko-Chieh Huang, Tzu-Wen Lin, Shui-Tein Chen, Kuo-Feng Hua(LPS) (L2630) was procured from Sigma‒Aldrich (St. Louis, MO). The CytoScan LDH Cytotoxicity Assay kit (786–210) was purchased from G-Bioscience (St. Louis, MO). Lastly, we employed an IL-1β ELISA kit purchased from

(5) Autophagy deficiency exacerbates iron overload induced reactive oxygen species production and apoptotic cell death in skeletal muscle cellsCell death & diseaseApril 7, 2023Sung HK, Murugathasan M, Abdul-Sater AA, Sweeney G.flow cytometry (Attune NxT) to assess eGFP fluorescence. Lactate dehydrogenase (LDH) assay LDH assay (#786-210, G-Biosciences) was used to determine cellular cytotoxicity of human skeletal muscle and L6 cells with

(6) "Cryopreserved human precision-cut lung slices provide an immune competent pulmonary test system for ""on-demand"" use and long-term cultures"Toxicological sciences : an official journal of the Society of ToxicologyFebruary 17, 2023Patel VS, Amin K, Wahab A, Marimoutou M, Ukishima L, Alvarez J, Battle K, Stucki AO, Clippinger AJ, Behrsing HP.was determined using CytoScan LDH Cytotoxicity Assay (G-Biosciences, St. Louis, Missouri [cat. no. 786-210]). In total, 50 μl of the samples (medium or lysates) were transferred to a 96-well plate and 50 µl

(7) Botanical inhibitors of SARS-CoV-2 viral entry: a phylogenetic perspectiveScientific ReportsJanuary 23, 2023Caitlin J. Risener, Sunmin Woo, Tharanga Samarakoon, Marco Caputo, Emily Edwards, Kier Klepzig, Wendy Applequist, Keivan Zandi, Shu Ling Goh, Jessica A. Downs-Bowen, et al.incubated for 48 h. Cytotoxicity was measured using a lactate dehydrogenase (LDH) assay (G Biosciences, #786–210) per manufacturer protocol. Additional cytotoxicity assays were run in a serial dilution at 512–2 µg/mL

(8) Controllable membrane damage by tunable peptide aggregation with albuminAIChE journal. American Institute of Chemical EngineersDecember 1, 2022Hamsici S, Gunay G, Acar H.control group. 4.11 | LDH Release LDH release was measured with Cytoscan-LDH Cytotoxicity Assay (G-BIOSCIENCES 786-210). Briefly, at the end of the treatments, collected supernatants were mixed with the reaction mixture

(9) PDIA3 inhibits mitochondrial respiratory function in brain endothelial cells and C. elegans through STAT3 signaling and decreases survival after OGDCell Communication and SignalingDecember 18, 2021Matt. P. Keasey, V. Razskazovskiy, C. Jia, E. D. Peterknecht, P. C. Bradshaw, T. Haggdehydrogenase release was used as an indicator of cell death and measured according to manufacturer’s protocol (786-210, G-Biosciences). Statistics Sample sizes were based upon power analysis (G*power) and indicate independent

(10) Modulation of Bax and Bcl-2 genes by secondary metabolites produced by Penicillium rubens JGIPR9 causes the apoptosis of cancer cell linesMycologyApril 3, 2021Prerana Venkatachalam, Varalakshmi Kilingar Nadumane(25 μg/ml) for 48 h was determined as per the kit manufacturer’s instructions (G-Biosciences, cat. # 786–210) (F K-M et al. 2013). Caspase −3, −7 and −10 activities Cancer cells (2x106 cells/ml) were treated

(11) Silymarin modulates catabolic cytokine expression through Sirt1 and SOX9 in human articular chondrocytesJournal of orthopaedic surgery and researchFebruary 20, 2021Wu WT, Chen YR, Lu DH, Senatov FS, Yang KC, Wang CC.mitochondrial activity of chondrocytes were evaluated using the lactate dehydrogenase (LDH) assay kit (786-210, CytoScan™, G-biosciences, USA) and the CCK8 reagent (TEN-CCK8, TOOLS Cell Counting CCK-8 kit, Tools,

(12) Anti-angiogenesis and apoptogenic potential of the brown marine alga, Chnoospora minimaFuture Journal of Pharmaceutical SciencesDecember 1, 2020Shabana Parveen, Varalakshmi K. Nadumaneassay The cytotoxicity was analysed by using Cytoscan™ LDH Assay method (G-Biosciences, India, kit #786-210) [8]. In this method the cytosolic enzyme lactate dehydrogenase (LDH) was measured in the cancer cells

(13) The proteasome activator PA200 regulates expression of genes involved in cell survival upon selective mitochondrial inhibition in neuroblastoma cellsJournal of cellular and molecular medicineJune 1, 2020Douida A, Batista F, Robaszkiewicz A, Boto P, Aladdin A, Szenykiv M, Czinege R, Virág L, Tar K.or untreated × 100. 2.5 LDH assay To assess necrosis, a CytoScan LDH Cytotoxicity Assay Kit (Cat.# 786‐210, G‐Biosciences) was used according to the manufacturer's instructions. Briefly, we quantitatively measured

(14) The Autophagy Machinery Controls Cell Death Switching between Apoptosis and NecroptosisDevelopmental cellMay 23, 2016Goodall ML, Fitzwalter BE, Zahedi S, Wu M, Rodriguez D, Mulcahy-Levy JM, Green DR, Morgan M, Cramer SD, Thorburn A.control wells. LDH release was quantitated using the Cytoscan-LDH Cytotoxicity Assay Kit (G-Biosciences, 786-210) according to manufacturer’s instructions and absorbance was read at 490nm using a Benchmark Plus microplate

(15) Regulation of autophagy and chloroquine sensitivity by oncogenic RAS in vitro is context-dependentAutophagyOctober 1, 2014Morgan MJ, Gamez G, Menke C, Hernandez A, Thorburn J, Gidan F, Staskiewicz L, Morgan S, Cummings C, Maycotte P, et al.of media was assayed from each well with the CytoscanTM-LDH Cytotoxicity Assay Kit (G-Biosciences, 786-210) according to the manufacturer’s instructions and the absorbance was read at 490 nm. Cell death percentages

(16) Inhibition of BRD4 attenuates tumor cell self-renewal and suppresses stem cell signaling in MYC driven medulloblastomaOncotargetMay 15, 2014Sujatha Venkataraman et al.bands. www.impactjournals.com/oncotarget 2357 Oncotarget Cytotoxicity Assay Kit- G Biosciences (Cat #786-210) was used. Briefly, media was collected from every time we changed the media on the culture and are

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CytoScan™ LDH Cytotoxicity Assay from G Biosciences

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CytoScan™ LDH Cytotoxicity Assay from G Biosciences

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