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MAO-Glo Assay
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MAO-Glo Assay
MAO-Glo Assay
MAO-Glo Assay from Promega
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Citations:
(29)
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MAO-Glo Assay
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Description
The MAO-Glo Assay provides a homogeneous luminescent method for measuring monoamine oxidase activity from recombinant and native sources and for testing the effects of test compounds on MAO activity
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
MAO-Glo Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V1402
Quantity
1,000 assays
Detection Target
monoamine oxidase (MAO)
Sample Type
Recombinant and native protein
Method
Luminescent
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (29)
(1)
(±)-Rutacycoumarins A and B: two pairs of unprecedented coumarin enantiomers from the aerial part of Ruta graveolens L. and chemically synthesized
Pharm Biol
December 13, 2025
Xu Feng, Jing Fang, Yanyang Liu, Chengyu Ge, Xiaolin Liao, Tao Jiang, Xiongjun Hou, Hao Huang, Shao Liu, Aimin Wang,
et al.
(Cat. No.
L4391
;
Sigma
), cell counting kit-8 (CCK-8; Cat. No. NU679; Dojindo), MAO GloTM
assay
kit (
V1402
;
Promega
), alanine transaminase (Cat. No. C009–2–1; Nanjing Jiancheng, China), and aspartate transaminase
(2)
The MAO‐B Inhibitor Selegiline Reduces the Viability of Different Prostate Cancer Cell Lines and Enhances the Effects of Anti‐Androgen and Cytostatic Agents
Pharmacology Research & Perspectives
October 1, 2025
Anita Steib, Krisztina Pohóczky, Norbert Tóth, Viktória Kormos, József Kun, Tamás Kálai, László Mangel, Péter Mátyus, Zsuzsanna Helyes
and MAO‐B enzyme activities were measured using the bioluminescent
MAO‐Glo assay
systems (cat. no.:
V1401
,
Promega
Corp, Madison, WI). Briefly, control cells and 100 μM selegiline‐treated cells were harvested
(3)
Renalase inhibition defends against acute and chronic β cell stress by regulating cell metabolism
Molecular Metabolism
May 1, 2025
Tara L. MacDonald, Birgitta Ryback, Jéssica Aparecida da Silva Pereira, Siying Wei, Bryhan Mendez, Erica P. Cai, Yuki Ishikawa, Meagan Arbeau, Gordon Weir, Susan Bonner-Weir,
et al.
was measured in
luminescence
units (RLU) using extra protocol steps specific to MAO-B (
Promega
cat.
V1401
); PG has been shown to
inhibit
this isoform specifically. Oral Pargyline (PG) administration: At day
(4)
Reinforcement and MAO-A inhibition in heated tobacco products: flavor and brand variations
Frontiers in Psychiatry
February 18, 2025
Xiangyu Li, Zheng Ding, Xingyi Jiang, Hongjuan Wang, Yanbo Luo, Huan Chen, Yongqiang Pang, Hongwei Hou, Qingyuan Hu
from TCI (catalog number N0080). The
MAO-Glo assay
system was acquired from
Promega
(catalog number
V1402
). The recombinant
human
MAO-A
, expressed in
baculovirus
infected BTI
insect
cells used in the study
(5)
Identification of Sarmentosin as a Key Bioactive from Blackcurrants (Ribes nigrum) for Inhibiting Platelet Monoamine Oxidase in Humans
Journal of Agricultural and Food Chemistry
July 31, 2024
Dominic Lomiwes, Catrin S. Günther, Stephen J. Bloor, Tania M. Trower, Nayer Ngametua, Alexander P. Kanon, Dwayne A. Jensen, Kim Lo, Greg Sawyer, Edward G. Walker,
et al.
deprenyl HCl on
MAO-A
and MAO-B, respectively, was measured using the MAO-Glo kit (
Promega
; Cat. No.
V1402
) according to the manufacturer’s instructions. The
IC50
of each compound in individually inhibiting
(6)
A neuronal action of sirtuin 1 suppresses bone mass in young and aging mice
The Journal of clinical investigation
December 1, 2022
Luo N, Mosialou I, Capulli M, Bisikirska B, Lin CS, Huang YY, Shyu PT, Guo XE, Economides A, Mann JJ,
et al
.
5 μg of mitochondrial protein sample was assayed in 96-well plates using the MAO-Glo Kit (
Promega
,
V1401
) according to the manufacturer’s instructions and as previously described (85). Briefly,
mouse
brains
(7)
Identification and Characterization of Novel Small-Molecule SMOX Inhibitors
Medical Sciences
August 30, 2022
Amelia B. Furbish, Ahmed S. Alford, Pieter Burger, Yuri K. Peterson, Tracy Murray-Stewart, Robert A. Casero, Patrick M. Woster
(M7316-1VL & M7441-1VL, Sigma-Aldrich, St. Louis, MO, USA) and a commercially available
assay
kit (#
V1401
, MAO-Glo,
Promega
, Madison, WI, USA), which utilizes the MAO-catalyzed oxidation of a luciferin derivative
(8)
Harmane Potentiates Nicotine Reinforcement Through MAO-A Inhibition at the Dose Related to Cigarette Smoking
Frontiers in Molecular Neuroscience
June 27, 2022
Zheng Ding, Xiangyu Li, Huan Chen, Hongwei Hou, Qingyuan Hu
membrane
before determination.
MAO-A
activity was determined by the
MAO-Glo™ Assay
Systems kit (
Promega
,
v1402
). 10 nM (1.7 ng/mL) rasagiline in DMSO (Shanghai yuanye, S24846) was added to each well to reduce the
(9)
LSD1 enzyme inhibitor TAK-418 unlocks aberrant epigenetic machinery and improves autism symptoms in neurodevelopmental disorder models
Science advances
March 1, 2021
Baba R, Matsuda S, Arakawa Y, Yamada R, Suzuki N, Ando T, Oki H, Igaki S, Daini M, Hattori Y,
et al
.
In vitro
monoamine oxidase (
MAO) A
/B
enzyme assay
MAOA/B enzyme activity was measured with
MAO-Glo Assay
(
V1402
,
Promega
) by following the manufacturer’s instructions (35). MAOA and MAOB were purchased from Sigma-Aldrich.
(10)
Microbial Metabolite Urolithin B Inhibits Recombinant Human Monoamine Oxidase A Enzyme
Metabolites
June 19, 2020
Rajbir Singh, Sandeep Chandrashekharappa, Praveen Kumar Vemula, Bodduluri Haribabu, Venkatakrishna Rao Jala
MAO-B enzyme isoforms were purchased from Sigma-Aldrich (St. Louis, MO, USA). A
MAO-Glo™ Assay
kit (
V1401
) was purchased from
Promega Corporation
(Madison, WI, USA). Chlorgylline (
MAO-A
inhibitor
) and deprenyl
(11)
Selection of Cells for Parkinson's Disease Cell-Therapy
International Journal of Stem Cell Research & Therapy
November 25, 2019
Chakraborty Ashok, Diwan Anil
oxidase-B) activity
assay
Mao-B activity
assay
was done using
MAO-Glo assay
Kit,
Promega
(Madison, WI, Cat#
V1401
) [23]. In brief, the
assay
was performed by incubating the cell homog• Page 3 of 13 • ISSN: 2469-570X DOI:
(12)
Comparison of monoamine oxidase inhibition by cigarettes and modified risk tobacco products
Toxicology Reports
November 13, 2019
Marco van der Toorn, Kyoko Koshibu, Walter K. Schlage, Shoaib Majeed, Pavel Pospisil, Julia Hoeng, Manuel C. Peitsch
their risk of dependence. 2 Materials and methods 2.1 Materials The
MAO-Glo assay
system (cat. no.
V1402
) was purchased from
Promega
(Dübendorf, Switzerland). The
human
MAOs used in this study were derived
(13)
Novel C7-Substituted Coumarins as Selective Monoamine Oxidase Inhibitors: Discovery, Synthesis and Theoretical Simulation
Molecules
November 5, 2019
Dong Wang, Ren-Yuan Hong, Mengyao Guo, Yi Liu, Nianhang Chen, Xun Li, De-Xin Kong
analogues were assayed by Shanghai ChemPartner Co. Ltd., using MAO-GloTM
assay
kit (
Promega Corporation
,
V1402
). Two
human
MAO enzymes (Cat#31502, Cat#31503) were purchased from Active Motif. Clorgyline and R(-)-deprenyl
(14)
Development of the triazole-fused pyrimidine derivatives as highly potent and reversible inhibitors of histone lysine specific demethylase 1 (LSD1/KDM1A)
Acta Pharmaceutica Sinica B
July 1, 2019
Zhonghua Li, Lina Ding, Zhongrui Li, Zhizheng Wang, Fengzhi Suo, Dandan Shen, Taoqian Zhao, Xudong Sun, Junwei Wang, Ying Liu,
et al.
from Active Motif (Cat#31502, Cat#31503). Biochemical kits were purchased from
Promega
(
MAO-Glo Assay
,
V1402
). Inhibition
assay
was carried out according to the manufacturer׳s protocol. The tested compound solution
(15)
Dual inhibitors of LSD1 and spermine oxidase
MedChemComm
May 1, 2019
Steven Holshouser, Matthew Dunworth, Tracy Murray-Stewart, Yuri K. Peterson, Pieter Burger, Joy Kirkpatrick, Huan-Huan Chen, Robert A. Casero, Patrick M. Woster
evaluated for the ability to
inhibit
monoamine oxidases (
MAO) A
and B using a commercial
assay
kit (#
V1401
,
Promega
Biosciences, Madison, WI). The results of this
assay
are displayed in Table 2. TCP inhibited
(16)
Targeted degradation of aberrant tau in frontotemporal dementia patient-derived neuronal cell models
eLife
March 25, 2019
Silva MC, Ferguson FM, Cai Q, Donovan KA, Nandi G, Patnaik D, Zhang T, Huang HT, Lucente DE, Dickerson BC,
et al
.
,
EZ-Link NHS-PEG4-Biotinylation Kit
,
Thermo Fisher Scientific
,Cat.
21455
, "Commercial
Assay
, Kit",
MAO-Glo Assay
Kit,
Promega
,Cat.
V1401
, "Commercial
Assay
, Kit",
Pierce BCA Protein Assay Kit
,
Thermo Fisher Scientific
,Cat.
23227
, "Commercial
(17)
Nicotine promotes neuron survival and partially protects from Parkinson's disease by suppressing SIRT6
Acta neuropathologica communications
November 8, 2018
Nicholatos JW, Francisco AB, Bender CA, Yeh T, Lugay FJ, Salazar JE, Glorioso C, Libert S.
β-actin. MAO-B activity MAO-B activity was analyzed by the commercial
MAO-Glo™ Assay
,
Promega
, Cat#
V1401
. Briefly, brain cortex homogenates were made in 100 mM HEPES 5% gylcerol buffer pH ~ 7.4. Homogenates
(18)
Design, Synthesis, and In Vitro Evaluation of Novel Histone H3 Peptide-Based LSD1 Inactivators Incorporating α,α-Disubstituted Amino Acids with γ-Turn-Inducing Structures
Molecules
May 6, 2018
Yosuke Ota, Taeko Kakizawa, Yukihiro Itoh, Takayoshi Suzuki
selectivity toward
LSD1
. For this investigation, we used a MAO-GloTM
Assay
System (
Promega
, Madison, WI, USA,
V1401
). Molecules 2018, 23, 1099 4 of 9 As shown in Table 1, all peptides did not inactivate
MAO-A
or
(19)
Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
PLOS Pathogens
February 9, 2018
Ning Zhang et al.
25 Aminomethyl benzoxaborole activation enzymatic pathway Amine oxidase activity
assay
MAO-Glo assay
(
Promega
,
V1401
) was applied to determine MAO and SSAO activities respectively according to manufacturer’s instructions.
(20)
Identification of JL1037 as a novel, specific, reversible lysine-specific demethylase 1 inhibitor that induce apoptosis and autophagy of AML cells
Oncotarget
May 9, 2017
Shuang Liu et al.
Sigma-Aldrich (St. Louis, MO, USA) and the inhibition
assay
was tested with
Promega
MAO-Glo™ Assay
kit (#
V1401
, Madison, WI, USA) according to the manufacturer's protocol. The luminescent signals were detected with
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MAO-Glo Assay from Promega
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