Calpain-Glo Protease Assay G8502 from Promega

Product Specs
ItemCalpain-Glo Protease Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8502
Quantity50ml
Detection TargetCalpain
MethodLuminescence
Molecular Weight~95kDa
TypeEnzyme

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Skeletal muscle myosin heavy chain fragmentation following exercise may be linked to post‐exercise inflammation and remodellingExp PhysiolFebruary 11, 2026Dakota R. Tiede, Diego Bittencourt, J. Max Michel, Daniel L. Plotkin, Madison L. Mattingly, Derick A. Anglin, Christopher G. Vann, Zachary A. Graham, Timothy J. Broderick, Marcas M. Bamman, et al.and SF isolates were assayed using commercially available kits (Promega, Madison, WI, USA; cat. nos G8502 and G8622) according to the manufacturer's instructions and as we have previously reported (Michel et

(2) Selective targeting of genome amplifications and repeat elements by CRISPR-Cas9 nickases to promote cancer cell deathNat CommunJune 2, 2025Matthew B. Hanlon, Jason M. Shohet, Scot A. Wolfewas measured at 3 days post-treatment with Cas9D10A using the Calpain-Glo™ Protease Assay (Promega, #G8501) according to the manufacturer’s instructions. Induction of calpain activation was achieved following

(3) Skeletal muscle myosin heavy chain fragmentation as a potential marker of protein degradation in response to resistance training and disuse atrophyExperimental PhysiologyOctober 1, 2024Daniel L. Plotkin, Madison L. Mattingly, Derick A. Anglin, J. Max Michel, Joshua S. Godwin, Mason C. McIntosh, Nicholas J. Kontos, João G. A. Bergamasco, Maíra C. Scarpelli, Vitor Angleri, et al.activity assays were performed using chemiluminescence assays (Promega Corp., Madison, WI, USA; cat. no.: G8502) similar to methods previously published by our laboratory (Scarpelli et al., 2024a), MyHCTotal fragmentation

(4) Skeletal muscle myosin heavy chain fragmentation as a potential marker of protein degradation in response to resistance training and disuse atrophyExp PhysiolAugust 24, 2024Daniel L. Plotkin, Madison L. Mattingly, Derick A. Anglin, J. Max Michel, Joshua S. Godwin, Mason C. McIntosh, Nicholas J. Kontos, João G. A. Bergamasco, Maíra C. Scarpelli, Vitor Angleri, et al.activity assays were performed using chemiluminescence assays (Promega Corp., Madison, WI, USA; cat. no.: G8502) similar to methods previously published by our laboratory (Scarpelli et al., 2024a), MyHCTotal fragmentation

(5) Myocardial SERCA2 Protects Against Cardiac Damage and Dysfunction Caused by Inhaled BromineJournal of Pharmacology and Experimental TherapeuticsJuly 1, 2024Juan Xavier Masjoan Juncos, Fahad Nadeem, Shazia Shakil, Malik El-Husari, Iram Zafar, William E. Louch, Ganesh V. Halade, Ahmed Zaky, Aftab Ahmad, Shama Ahmadcardiac tissue lysates prepared as previously published using commercial calpain activity assay kits (G8501, Promega, Madison, WI) (Ahmad et al., 2019). Western blots were performed in cardiac tissue lysates

(6) Protective Effects of Estrogen via Nanoparticle Delivery to Attenuate Myelin Loss and Neuronal Death after Spinal Cord InjuryNeurochemical researchNovember 1, 2021Haque A, Drasites KP, Cox A, Capone M, Myatich AI, Shams R, Matzelle D, Garner DP, Bredikhin M, Shields DC, et al.in triplicate in 96-well flat-bottomed plates using Calpain-Glo™ Protease Assay Kit (Promega G8501, G8502) following manufacturer’s instructions. Plates were incubated at 37 °C for 5 min as indicated. Luminescence

(7) PAR-4/Ca(2+)-calpain pathway activation stimulates platelet-derived microparticles in hyperglycemic type 2 diabetesCardiovascular diabetologyApril 3, 2021Giannella A, Ceolotto G, Radu CM, Cattelan A, Iori E, Benetti A, Fabris F, Simioni P, Avogaro A, Vigili de Kreutzenberg S.Italy). Calpain activity assay Calpain activity was determined by Calpain-Glo™ Protease Assay (cat# G8502, Promega, Madison, USA) according to the manufacturer’s instructions. Briefly, washed platelet (400

(8) Cisplatin induced neurotoxicity is mediated by Sarm1 and calpain activationScientific ReportsDecember 14, 2020Aysel Cetinkaya-Fisgin, Xinghua Luan, Nicole Reed, Ye Eun Jeong, Byoung Chol Oh, Ahmet Hokeproportional with luminescence was measured with a luminometer plate reader. Since Calpain-Glo (Promega, G8501) is designed for purified enzyme activity, we modified the original protocol to detect calpain activity

(9) Chalcomoracin is a potent anticancer agent acting through triggering Oxidative stress via a mitophagy- and paraptosis-dependent mechanismScientific reportsJune 22, 2018Han H, Chou CC, Li R, Liu J, Zhang L, Zhu W, Hu J, Yang B, Tian J.calpain 1 and 2 protease activity was evaluated using a Calpain-GloTM protease assay kit (Promega, G8501). Briefly, MDA-MB-231, PC-3 and LNCaP cells were seeded into 24-well plates and then treated with a

(10) Interleukin-33 is activated by allergen- and necrosis-associated proteolytic activities to regulate its alarmin activity during epithelial damageScientific reportsFebruary 20, 2018Scott IC, Majithiya JB, Sanden C, Thornton P, Sanders PN, Moore T, Guscott M, Corkill DJ, Erjefält JS, Cohen ES.Calpain protease activity in BAL samples was quantified using Calpain-Glo luminescent assay (Promega G8501) according to manufacturer’s instructions. Biochemical IL-33 assays Human rFL IL-33 lysate, mock lysate

(11) DCAF7/WDR68 is required for normal levels of DYRK1A and DYRK1BPloS oneJanuary 1, 2018Yousefelahiyeh M, Xu J, Alvarado E, Yu Y, Salven D, Nissen RM.(3483-12-3, Thermo Fisher Scientific), CaCl2 (10035-04-8, Fisher Scientific), Calpain-Glo Protease Assay (G8501, Promega). Antibodies used were anti-WDR68 (HPA022948, Sigma-Aldrich), anti-DYRK1A (8765S, Cell Signaling), anti-DYRK1B

(12) Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung DiseaseEBioMedicineSeptember 1, 2017White MM, Geraghty P, Hayes E, Cox S, Leitch W, Alfawaz B, Lavelle GM, McElvaney OJ, Flannery R, Keenan J, et al.quantified in neutrophil cytosols using Calpain-Glo™ Protease Assay (Promega, WI, USA, product code G8501) (Reeves et al., 2013). Reagents were prepared and the protocol performed as per the manufacturer's

(13) A calcium- and calpain-dependent pathway determines the response to lenalidomide in myelodysplastic syndromesNature medicineJuly 1, 2016Fang J, Liu X, Bolanos L, Barker B, Rigolino C, Cortelezzi A, Oliva EN, Cuzzola M, Grimes HL, Fontanillo C, et al.(Bio-Rad). Calpain activity Calpain activity was measured according to the manufacturer’s instructions (G8501; Promega). Briefly, cells were washed in cold calcium-free 1× PBS and lysed in 10 mM HEPES (pH 7.2),

(14) The combination of lithium and l-Dopa/Carbidopa reduces MPTP-induced abnormal involuntary movements (AIMs) via calpain-1 inhibition in a mouse model: Relevance for Parkinson׳s disease therapyBrain ResearchOctober 5, 2015Carol A. Lazzara, Rebeccah R. Riley, Anand Rane, Julie K. Andersen, Yong-Hwan Kimcalpain 1 and 2 activities and other steps were followed as recommended by the manufacturer (Promega G8501, Madison, WI). Due to the limit of collecting high concentration of total protein from the midbrain

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Calpain-Glo Protease Assay

Calpain-Glo Protease Assay from Promega

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Calpain-Glo Protease Assay from Promega

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