Proteasome-Glo Chymotrypsin-Like Cell-Based Assay G8660 from Promega

Product Specs
ItemProteasome-Glo Chymotrypsin-Like Cell-Based Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8660
MethodLuminescent
Sample TypeCells
TypeEnzyme
Quantity10ml
Detection TargetProteasome

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegenerationCell Stem CellFebruary 5, 2026Nathalie Saurat, Andrew P. Minotti, Maliha T. Rahman, Trisha Sikder, Chao Zhang, Daniela Cornacchia, Johannes Jungverdorben, Gabriele Ciceri, Doron Betel, Lorenz StuderPierce Reversible Protein Stain Kit for PVDF Membranes,Thermo Fisher Scientific,24585 Cell-Based Proteasome-Glo™ Assay kit,Promega,G8660 ,, Deposited data,Deposited data,Deposited data ,, RNAseq data. Young and Old Cortex,Zhang et al.35,PRJNA1107241 Tabula

(2) Ablation of LAT2 Transporter Causes Intramuscular Glutamine Accumulation and Inhibition of Fasting-Induced ProteolysisJ Cachexia Sarcopenia MuscleJune 23, 2025Meritxell Espino-Guarch, Susie Shih Yin Huang, Clara Vilches, Esther Prat, Rana El Nahas, Ghalia Missous, Susanna Bodoy, Abbirami Sathappan, Mohammad Ameen Al-Aghbar, Clara Mayayo, et al.using the following Luminescent Promega Assays: Proteasome‐Glo Chymotrypsin‐Like Cell‐Based Assay (G8660), Proteasome‐Glo Trypsin‐like cell‐Based Assay (G8760) and Cell‐Based Proteasome‐Glo Assay Caspase like

(3) A novel indirubin- 3-monoxime derivative I3MV- 8b exhibits remarkable cytotoxicity against multiple myeloma by targeting TRIM28Biomark ResApril 7, 2025Teng Fang, Lanting Liu, Hao Sun, Xiaoyu Zhang, Xiyue Sun, Zhen Yu, Lixin Gong, Shiyi Xie, Yonglong Zhao, Yan Li, et al.activity assay Proteasome-Glo™ Chymotrypsin-Like, Caspase-Like, and Trypsin-Like Cell-Based Assays (G8660, Promega Corporation, Madison, USA) were used to conduct proteasome activity assays following the manufacturer’s

(4) 1'-Acetoxychavicol Acetate Selectively Downregulates Tumor Necrosis Factor Receptor-Associated Factor 2 (TRAF2) ExpressionMoleculesMarch 10, 2025Chihiro Moriwaki, Shingo Takahashi, Nhat Thi Vu, Yasunobu Miyake, Takao KataokaAssay Proteasome activities were measured using the proteasome-GloTM Chymotrypsin-Like Cell-based Assay (G8660) (Promega, Madison, MI, USA) and the Proteasome-GloTM Trypsin-Like Cell-based Assay (G8760) (Promega).

(5) Proteogenomic analysis reveals adaptive strategies for alleviating the consequences of aneuploidy in cancerThe EMBO JournalFebruary 10, 2025Jan-Eric Bökenkamp, Kristina Keuper, Stefan Redel, Karen Barthel, Leah Johnson, Amelie Becker, Angela Wieland, Markus Räschle, Zuzana Storchová,Thermo Fisher Scientific,25200056 CellTiter-Glo®,Promega,G7570 Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay,Promega,G8660 Bradford protein assay,Biorad,5000006 Clarity™ Western ECL substrate solution,Biorad,#1705061 DNA

(6) Anthricin-induced hyperactive proteasome and its molecular mechanismBiochemistry and Biophysics ReportsDecember 1, 2024Kotaro Sakamoto, Runa Fujimoto, Erina Kamiyama-Ando, Takatsugu Hirokawa(043–29791, FUJIFILM Wako). The proteasome activity was estimated using the Proteasome-Glo Cell-Based Assay (G8660, Promega, WI, USA) in accordance with the manufacturer's instructions. Luminescence was detected using

(7) Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegenerationCell stem cellAugust 1, 2024Saurat N, Minotti AP, Rahman MT, Sikder T, Zhang C, Cornacchia D, Jungverdorben J, Ciceri G, Betel D, Studer L.were used to assay for proteosome function using the Cell-Based Proteasome-Glo™ Assay kit (Promega G8660) as described by the manufacturer. In brief the reagents were prepared and left to equilibrate at room

(8) 20S proteasome enhancers prevent cytotoxic tubulin polymerization-promoting protein induced α-synuclein aggregationiScienceJuly 19, 2024Sophia D. Staerz, Charles Anamoah, Jetze J. TepeFluorescent probe,R&D Systems,I-190 RP1 Probe,"Gaur, P.; et al.70",N/A Proteasome Glo Reagent,Promega,G8660 Cell Titer Glo Reagent,Promega,G3580 TCH-165,"Njomen, E.; et al.41",N/A Bortezomib,Cayman Chemicals,10008822 HisTrap HP

(9) Single-nucleus sequencing reveals enriched expression of genetic risk factors in extratelencephalic neurons sensitive to degeneration in ALSNat AgingJuly 1, 2024Limone F, Mordes DA, Couto A, Joseph BJ, Mitchell JM, Therrien M, Ghosh SD, Meyer D, Zhang Y, Goldman M, et al.of maturation, treated for 24 h. Cells were washed with 1× PBS, exposed to ProteasomeGlo (Promega, G8660) and incubated for 30 min at room temperature. Fluorescence was measured using a Cytation 3 reader (BioTek). Statistics

(10) New hemisynthetic derivatives of sphaeropsidin phytotoxins triggering severe endoplasmic reticulum swelling in cancer cellsScientific ReportsJune 25, 2024Aude Ingels, Robert Scott, Annie R. Hooper, Aletta E. van der Westhuyzen, Sachin B. Wagh, Joséphine de Meester, Lucia Maddau, Doris Marko, Georg Aichinger, Walter Berger, et al.chemotrypsin-like, trypsin-like and caspase-like activities of the proteasome were evaluated in cells with the G8660, G8861 and G8761 kits respectively according to the manufacturer’s instructions (Promega, USA). Briefly,

(11) Toll-like receptor signaling in multiple myeloma cells promotes the expression of pro-survival genes B-cell lymphoma 2 and MYC and modulates the expression of B-cell maturation antigenFrontiers in ImmunologyJune 7, 2024Synne Stokke Tryggestad, Ingrid Aass Roseth, Kristin Roseth Aass, Nadia Elise Helene Ørning, Robin Mjelle, Hanne Hella, Therese Standalassay Chymotrypsin-like proteasome activity was measured using Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay (#G8660, Promega) according to the manufacturer’s protocol. RPMI-8226 cells were seeded at a concentration of

(12) Induction of proteasomal activity in mammalian cells by lifespan-extending tRNA synthetase inhibitorsGeroScienceApril 1, 2024Mariner BL, Rodriguez AS, Heath OC, McCormick MA.element [66, 67]. Proteasomal assays utilized (1) Promega’s Proteasome-Glo Caspase-like Cell-Based Assay (G8660) and (2) Abcam’s Proteasomal Activity Kit (ab107921) according to the manufacturers’ procedures respectively

(13) CRIP1 involves the pathogenesis of multiple myeloma via dual-regulation of proteasome and autophagyeBioMedicineFebruary 1, 2024Peixia Tang, Zhen Yu, Hao Sun, Lanting Liu, Lixin Gong, Teng Fang, Xiyue Sun, Shiyi Xie, Gang An, Zhenshu Xu, et al.assays were conducted using Proteasome-GloTM Chymotrypsin-Like and Caspase-Like Cell-Based Assays (G8660, Promega Corporation, Madison, WI, USA) according to the manufacturer's protocol. RNA sequencing RNA sequencing

(14) Rational drug combination design in patient-derived avatars reveals effective inhibition of hepatocellular carcinoma with proteasome and CDK inhibitorsJournal of Experimental & Clinical Cancer ResearchAugust 15, 2022Jhin Jieh Lim, Lissa Hooi, Yock Young Dan, Glenn K. Bonney, Lei Zhou, Pierce K.-H. Chow, Cheng Ean Chee, Tan Boon Toh, Edward K.-H. Chowhours and 24 hours post-treatment using Proteasome-Glo Chymotrypsin-Like Cell-Based Assay (Promega, G8660) as per manufacturer’s instructions. RNA isolation and quantitative RT-PCR After 48 hours of drug

(15) A protein-protein interaction map reveals that the Coxiella burnetii effector CirB inhibits host proteasome activityPLoS pathogensJuly 1, 2022Fu M, Liu Y, Wang G, Wang P, Zhang J, Chen C, Zhao M, Zhang S, Jiao J, Ouyang X, et al.appointed time points, the cells were detected using a proteasome activity detection kit (Promega, G8660). Briefly, the assay buffer was prepared by dissolving luciferin detection reagent into the Proteasome-Glo

(16) Modulation of the 20S Proteasome Activity by Porphyrin Derivatives Is Steered through Their Charge DistributionBiomoleculesMay 24, 2022Marco Persico et al.intact MCF7 cells was determined using the bioluminescent Proteasome-Glo™ cell-based assay system (G8660; Promega, Mannheim, Germany) according to manufacturer’s instructions. Briefly, 800 cells/well were

(17) Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myelomaEBioMedicineApril 1, 2022Yu Z, Wei X, Liu L, Sun H, Fang T, Wang L, Li Y, Sui W, Wang K, He Y, et al.activity assays were conducted using Proteasome-Glo™ Chymotrypsin-Like and Caspase-Like Cell-Based Assays (G8660, Promega Corporation, Madison, USA), respectively, according to the manufacturer's protocol. Primers

(18) Hsf1 promotes hematopoietic stem cell fitness and proteostasis in response to ex vivo culture stress and agingCell stem cellNovember 4, 2021Kruta M, Sunshine MJ, Chua BA, Fu Y, Chawla A, Dillingham CH, Hidalgo San Jose L, De Jong B, Zhou FJ, Signer RAJ.azide,Life Technologies,Cat #A20012 Proteasome-Glo chymotrypsin-like cell based assay,Promega,Cat #G8660 RNeasy Plus Micro Kit,Qiagen,Cat #74034 Deposited Data,Deposited Data,Deposited Data RNA-sequencing

(19) Cisplatin induced neurotoxicity is mediated by Sarm1 and calpain activationScientific ReportsDecember 14, 2020Aysel Cetinkaya-Fisgin, Xinghua Luan, Nicole Reed, Ye Eun Jeong, Byoung Chol Oh, Ahmet Hokethe wells and protease assays are performed every 4 h for a total of 48 h. Proteasome-Glo (Promega, G8660) kit is used to assay for chymotrypsin-like proteasome activity, and Caspase-Glo 3/7 (Promega, G8091)

(20) USP7 regulates ALS-associated proteotoxicity and quality control through the NEDD4L-SMAD pathwayProceedings of the National Academy of Sciences of the United States of AmericaNovember 10, 2020Zhang T, Periz G, Lu YN, Wang J.After 4 h, the proteasome activity assay was performed by using the proteasome-Glo Cell-Based kit (G8660; Promega), and luminescence was measured by using an injector-equipped Synergy H1 microplate reader

Write a Review

(1)

8/7/2023Assay Kit to Measure Proteasome Activity From Promega Shivali RawatThe proteasome is the main protein degradation machinery in cells. To study the effect of proteasome inhibition on the cellular proteome, I used an inhibitor that blocks the chymotrypsin-like activity... Read Review

Proteasome-Glo Chymotrypsin-Like Cell-Based Assay

Proteasome-Glo Chymotrypsin-Like Cell-Based Assay from Promega

Description

Promega

Citations (42)

Reviews

Proteasome-Glo Chymotrypsin-Like Cell-Based Assay from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map