Griess Reagent System G2930 from Promega

Product Specs
ItemGriess Reagent System
CompanyPromega
Price $106.00Supplier Page View Company Product Page
Catalog NumberG2930
Detection TargetNitrite
Quantity1,000 assays
MethodColorimetric
Sample Typeplasma, serum, urine and tissue culture medium
Sensitivity2.5mM (125pmol) nitrite (in ultrapure, deionized, distilled water)
Molecular Weight~69kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Monophosphoryl lipid A boosts macrophage antimicrobial immunity by metabolically regulating source-specific ROS generationFrontiers in ImmunologyMarch 30, 2026Dan Hao, Benjamin D. Klein, Margaret A. McBride, Julia K. Bohannon, Naeem K. Patil, Xenia D. Davis, Mary A. Oliver, Mei Lin Ning Dye, Sara Weidenbach, Jamey D. Young, et al.levels in cell culture supernatants (1 ×106) were quantified using the Griess Reagent System (Promega, G2930) according to the manufacturer’s instructions. Absorbance was measured at 520 nm. Xanthine oxidase

(2) FSH Receptor Asn680Ser Polymorphism Modulates Intrafollicular Nitric Oxide Bioavailability and Ovarian Responsiveness During IVFInt J Mol SciMarch 6, 2026Charalampos Voros, Diamantis Athanasiou, Despoina Mavrogianni, Ntilay Soyhan, Georgia Panagou, Maria Sakellariou, Georgios Papadimas, Fotios Chatzinikolaou, Eleni Sivylla Bikouvaraki, Georgios Daskalakis, et al.indicating the availability of nitric oxide. We used a commercial Griess Reagent System (Promega, Catalogue #G2930) to quantify the total nitric oxide metabolites (NOx = NO3− + NO2−). Calibration curves were constructed

(3) Microplastic-Induced Macrophage Dysfunction Drives Lung Tumor Progression through Glutathione ImbalanceACS NanoFebruary 24, 2026Bora Kim, Koung-Min Park, Haerang Lee, Young-Min Hyunin the dark. Subsequently, 50 μL of N-1-naphthylethylenediamine dihydrochloride solution (Promega, G2930) was added to each well, and the cells were further incubated for 10 min under identical conditions.

(4) Combined Flexion, Torsion and Compression Drive Distinct Intervertebral Disc Failure Mechanisms Under Asymmetric, High-Cycle LoadingJOR SpineFebruary 11, 2026Amra Šećerović, Aapo Ristaniemi, Francesco Crivelli, Sarah Heub, Mauro Alini, Gilles Weder, Diane Ledroit, Stephen J. Ferguson, Sibylle Grad1,9‐dimethyl‐methylene blue assay. NO release was measured using the Griess Reagent System (Promega, USA; #G2930), and interleukin‐6 (IL‐6) and interleukin‐8 (IL‐8) releases were quantified using bovine‐specific ELISA

(5) Anti-Inflammatory Effect of Excretion-Secretion Products of Clinostomum marginatum (Digenea: Clinostomidae) and Its Effect over the Viability and Antioxidative Activity of a Mix of Lactobacillus and/or BifidobacteriumMicroorganismsFebruary 3, 2026María Angélica Gutiérrez-Nava, Raquel González-Vázquez, Miguel Ángel Mosqueda-Cabrera, Daniela Reyna-González, Felipe Mendoza-Pérez, Eduardo Zúñiga-León, Leovigildo Mateos-Sánchez, Pedro A. Reyes-Castillo, Rosa González-Vázquez, María Guadalupe Córdova-Espinoza, et al.Quantification NO production in the culture supernatants was determined using the Griess reagent (PROMEGA G2930 kit, Promega, Madison, WI, USA) according to the manufacturer’s instructions. A standard curve was generated

(6) Pharmacological characterization and preclinical evaluation of 11h: a novel, brain-penetrant PDE4 inhibitor for neurological disordersFrontiers in PharmacologyJanuary 8, 2026Jacob Lackovic, Sougata Dey, Sara Jane Ward, Nigel H. Greig, David Tweedie, Nassim Beiranvand, Uksha Saini, Dev Chatterjee, Atul VaradhacharyNitrite levels were assessed by the use of the Griess Reagent System (Promega, Madison, WI, USA, Cat # G2930) or the Nitrate/Nitrite Fluorometric Assay Kit, (Abnova, Cat # KA1344). Statistical analysis was performed

(7) Macrophage Subtypes Distinctly Impact Intervertebral Disc Inflammation and Extracellular Matrix CompositionJOR SpineDecember 12, 2025Lauren E. Lisiewski, Leonardo Campos, Timothy D. Jacobsen, Min Kyu M. Kim, Nadeen O. Chahinemeasuring nitric oxide (NO) release into the media using the Griess Reagent System (Promega, Cat#. G2930). All NO release results are presented as cumulative release over the entirety of the culture period

(8) Chronic compression induces transcriptional, metabolic, and functional state changes in macrophages that recapitulate tumor-associated phenotypesFront ImmunolDecember 9, 2025Alice Burchett Darantiere, Hao Chen, Julian Najera, Scott Howard, Meenal Dattacompressed macrophages was analyzed using a Griess reagent system following the vendor protocol (Promega, G2930). RNA sequencing and bioinformatics analysis RNA was extracted as described above from six samples

(9) Synergistic Anticancer Activity of Fucoidan from Lessonia trabeculata Combined with Chemotherapeutic Agents in 4T1 Breast SpheroidsMarine DrugsNovember 26, 2025Rosa María Condori Macuri, Libertad Alzamora-Gonzales, Erasmo Honorio Colona-Vallejos, Raisa Teresa Cruz Riquelme, Laura Inés Pecho Chávez, Jherson Oscar Cisneros Gutierrez, Victor Alonso Montejo AnlasFrance, cat# 15290018), Griess Reagent System was purchased from Promega (Fitchburg, WI, USA, cat# G2930), and Ketamine/xylacine Injectable Solution for veterinary use was purchased from Ket-A-Xyl® (Lima,

(10) Host Translational Control by Stress Granules Promotes Mycobacterium tuberculosis PathogenesisMedComm (2020)November 10, 2025Jaewhan Kim, Sang‐Hun Son, Ji‐Ae Choi, Junghwan Lee, Seoyeon Jo, Soo‐Na Cho, Doan Tam Nguyen, Doyi Son, Kee K. Kim, Chang‐Hwa Songbreakdown product of NO, following the manufacturer's instructions for the Griess Reagent System (Promega #G2930). BMDM culture media were filtered and added to a 96‐well plate in triplicate. A standard solution was

(11) Lifespan‐Regulated CAR‐Macrophages from Myeloid Progenitors for Enhanced Colorectal Cancer TherapyAdvanced ScienceNovember 1, 2025Chuancheng Gao, Fangling Hong, Yao Dong, Yong Fu, Yunong Ma, Xuedi Sun, Junfeng Zhang, Jiangning Chen, Zhen Huangprobe (cat. no.: S0033, Beyotime, Shanghai, China) and using the Griess Reagent System kit (cat. no.: G2930, Promega, Wisconsin, USA) according to the manufacture's instruction. The apoptosis of tumor cells was

(12) Nitric oxide regulates cytochrome P450 2D6 and 3A4 activity via concentration-dependent modulation of heme loadingJournal of Biological ChemistryNovember 1, 2025Priya Das Das Sinha, Sidra Islam, Pranjal Biswas, Dennis J. Stuehrwere collected, and nitrite accumulation in this medium was measured using the Griess reagent system (G2930; Promega). The plates were then harvested using cold lysis buffer (40 mM EPPS, 150 mM NaCl, 10% glycerol,

(13) Differential Profile of Hemostasis in Dengue Fever Before and After COVID-19VirusesOctober 28, 2025Alanna Calheiros Santos, Julya Farias Carneiro, Anna Paula de Souza e Silva Sales, Mariana Gandini, Flávia Barreto dos Santos, Paulo Vieira Damasco, Luis José de Souza, Elzinandes Leal de Azeredo, Luzia Maria de-Oliveira-Pintooxide (NO) was performed indirectly by detecting nitrite (NO2−) using the Griess reagent kit (Cat. no. G2930, Promega), according to the manufacturer’s instructions. 2.5. Enzyme-Linked Immunosorbent Assay for

(14) Interleukin 10 controls the balance between tolerance, pathogen elimination, and immunopathology in birdsElifeOctober 16, 2025Dominique Meunier, Ricardo Corona-Torres, Kay Boulton, Zhiguang Wu, Maeve Ballantyne, Laura Glendinning, Anum Ali Ahmad, Dominika Borowska, Lorna Taylor, Lonneke Vervelde, et al.nitrite levels were measured by Griess assay, according to the manufacturer’s instructions (Promega G2930); absorbance was measured at 550 nm. Statistical significance was calculated using two-tailed unpaired

(15) Differential Effects of Selenium Compounds on Mitochondrial Function in PRRSV-Infected Porcine Alveolar MacrophagesVirusesSeptember 26, 2025Abigail Williams, Christina Bourne, John Byrne, Chaitawat Sirisereewan, Brittany M. Pecoraro, Elisa Crisciwas analyzed using the Griess Reagent System following the manufacturer’s instructions (Catalog No. G2930, Promega, Madison, WI, USA). Briefly, PAMs were washed and seeded at 150,000 cells per well with RPMI

(16) Sex differences in the redox response to fine particulate matter (PM 2.5) air pollution protects female mice against metabolic and cardiac injuryPhysiological ReportsSeptember 2, 2025Michael Kitching, Amanda Ribble, Ashley Wright, Aruni Bhatnagar, Petra HaberzettlPeroxidation (MDA) Colorimetric Assay Kit, United States, ab118970; Griess assay, Promega, United States G2930). While MDA, a lipoperoxidation product, is commonly used as a marker for oxidative stress, the abundance

(17) Methods for Evaluating the Effects of 2D and 3D Culture Environment on Macrophage Response to Mycobacterium InfectionMicroorganismsAugust 29, 2025Danielle L. Stolley, Komal S. Rasaputra, Elebeoba E. Maymatrix lysates were stored at −80 °C until used to quantify nitric oxide (NO; Griess assay Promega™, G2930, Madison, WI, USA) and cell death (LDH cytotoxicity assay; Pierce™, 88954, Thermo Fisher Scientific,

(18) ALS/FTD-linked TBK1 deficiency in microglia induces an aged-like microglial signature and drives social recognition deficits in miceNature communicationsAugust 26, 2025Lenoel I, Ribon M, Lorenc F, Diebold A, Philibert CE, Robaldo D, Badsi M, Perronnet J, Lameth J, Berriat F, et al.assay Nitrite concentration in supernatants were determined using the Griess Reagent System (Promega, G2930) according to manufacturer’s instructions and the absorbance at 540 nm was measured with Spectramax

(19) Extracellular vesicles from Distinct Histoplasma capsulatum Strains Modulate Phagocyte Function and Promote Fungal PersistenceACS Infectious DiseasesAugust 8, 2025Taiane N. Souza, Alessandro F. Valdez, Ana Claudia G. Zimbres, Bianca A. G. Sena, Flavia C. G. Reis, Marcio L. Rodrigues, Daniel Zamith-Miranda, Allan J. Guimarães, Alessandra A. Filardy, Joshua D. Nosanchuk, et al.quantification, as an indicator of NO production, was measured using the Griess reagent (Promega catalog no. G2930). Western Blot BMDM (1 × 106 cells/well) was treated overnight with EVs isolated from G-217B or G-184A

(20) STAT1 and STAT6 orchestrate Cbs transcription and transsulfur metabolism in microglia and contribute to parkinson’s disease-related neuroinflammationCellular and Molecular Life SciencesJuly 30, 2025Yang Liu, Zhen Wang, Ya-Ting Ma, Yu-Xuan Chen, Xin-Yi Qian, Xiao-Ou Hou, Bo Wan, Hai-Gang Ren, Li-Fang Hu(Shanghai, China). Nitric oxide (NO) measurement NO was measured by nitrite levels via the Griess reagent (G2930, Promega, USA). To measure nitrite, 50 µl of phenol red-free culture supernatant was sequentially added

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(1)

12/10/2020Fast and Sensitive Nitric Oxide Assay Pauline MorinThe levels of nitric oxide in cell culture supernatants (LPS-inflamed RAW267.4) were measured using the Griess Reagent Assay. The test was fast to perfom, the instructions were easy and the test very ... Read Review
(1)

2/25/2015Using Griess Reagent System to measure Nitrite release from BV-2 cells Rebecca WilshusenThe goal of my research is to stimulate BV-2 cells (microglial cell line) with N-4YSyn (tail end of the antigenic protein found in Parkinson's disease) and then evaluate the BV-2 cells for increased ... Read Review

Griess Reagent System

Griess Reagent System from Promega

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Griess Reagent System from Promega

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