Griess Reagent System G2930 from Promega

Product Specs
ItemGriess Reagent System
CompanyPromega
Price $106.00Supplier Page View Company Product Page
Catalog NumberG2930
Quantity1,000 assays
MethodColorimetric
Sample Typeplasma, serum, urine and tissue culture medium
Sensitivity2.5mM (125pmol) nitrite (in ultrapure, deionized, distilled water)
Molecular Weight~69kDa
Detection TargetNitrite

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1Redox BiologyJune 1, 2026Nicholas L. Arp, Uzziah S. Urquiza, Marcel Morgenstern, Jonathan H. Schrope, James A. Votava, Steven V. John, Jack J. Stevens, Felicia Deng, Anna Huttenlocher, Joshua J. Coon, et al.concentrations Nitrite concentrations in conditioned media were measured using the Griess Reagent System (Promega, G2930) according to the manufacturer's instructions. Briefly, 50 μL of conditioned media was combined with

(2) Piezoelectric cold atmospheric plasma modulates neurogenic cell proliferation and differentiation via cross-signalingIBRO Neuroscience ReportsJune 1, 2026Haitao Zhang, Boyu Cai, Jiaojiao Li, Zhao Wang, Maoxiang Xu, Ding Li, Fei Tanof nitric oxide (NO) in the cell-free medium was assessed using the Griess Reagent System (Promega, #G2930). Briefly, 100 µL of the plasma-treated medium was transferred to a 96-well plate and mixed with 100

(3) Long‐Term Stability of Bacterial Extracellular Vesicles Stored at Different TemperaturesJournal of Extracellular BiologyMay 25, 2026Anagha Rama Varma, Frank Borris, Parnika Kant, Kaitlyn Sadtler, Parinaz Fathiwere purchased from Fisher Scientific. The Griess assay reagent systems were purchased from Promega (G2930). Pierce BCA Protein Assay Kits (Thermo Scientific 23227) were used for protein concentration quantification.

(4) Downregulation of Caveolin-2 Is a Novel Mechanism Involved in Activation of Inducible Nitric Oxide Synthase (iNOS) in Mouse Peritoneal MacrophagesMediators InflammMay 25, 2026Sungchan Jang, Grzegorz Sowaof nitrite in culture media, a stable metabolite of NO using Griess Reagent System (Promega; Cat.# G2930) according to manufacturer’s instructions. The data are expressed as the mean nitrite concentration

(5) Redox signaling modulates nitrite transport in a commensal StreptococcusRedox BiolMay 6, 2026Sara E. Edmonds, Joshua J. Baty, Jessica A. Scoffieldsamples to assess intracellular and extracellular nitrite using the Griess Reagent kit (Promega, Cat #: G2930) per the manufacturer's instructions. Intracellular nitrite concentrations were obtained by normalizing

(6) Protective effects of Lactiplantibacillus plantarum YC225 Mixture Are associated with Gut Microbiota in Vulvovaginal CandidiasisJournal of Microbiology and BiotechnologyApril 27, 2026Hyun Ju Kim, Dong-Gu Kim, Se-Woong Go, Jung-Hwang Park, Jong-Sik Jin, Hoonsung Choi, Ji-Hye Ahn, Iibum Park, Eui-Seong Parkconcentrations of IL-6 (#ab100712, Abcam, UK), MPO activity (#EK0943; Boster Bio Technology, USA), and NO (#G2930, Promega, USA) were measured using commercial ELISA and assay kits. Quantification of C. albicans in

(7) Gnetum montanum extract attenuates lipopolysaccharide induced acute lung inflammation through Nrf2 and heme oxygenase 1 mediated redox modulation in macrophagesScientific ReportsApril 24, 2026Duc-Vinh Pham, Hong-Linh Tran, Thu-Hang Nguyen, Hong-Nhung Bui, Hai-Nam Nguyen, Thanh Nguyen Le, Thi Hien Pham, Thuy-Duong NguyenTNF-α assays. NO levels were quantified using a modified Griess method with the Griess Reagent System (#G2930, Promega). Concentrations of IL-6, TNF-α, and PGE2 were determined by ELISA using commercial kits from

(8) Effect of Bean-Derived Soluble Dietary Fibers on Macrophage Function In VitroFoodsApril 23, 2026Ana M. Magallanes López, Mark Williamson, Senay Simsek, Estelle Leclerckit (MLB00C) were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). The Griess Reagent System (G2930) was obtained from Promega Co. (Madison, WI, USA). The PureLink RNA Mini Kit (12183018A) was obtained

(9) Dendritic Cells Derived MMP9 Fuels Sepsis-Induced Cardiac Dysfunction Through JNK/AP1 Driven Pro-Inflammatory Cytokine and Nitric Oxide StormInflammationApril 22, 2026Jiewen Deng, Yulan Qu, Yanyan Jiang, Yao Xie, Xiaotian Ma, Jian Shen, Zhenhong Guo, Meixiang Xiangrespectively. Nitrite Assay Cell culture supernatants or the mouse sera (50 µl) were mixed with Griess reagent (G2930, Promega, Madison, WI) in a 96-well plate according to the manufacturer’s protocol. The absorbance of

(10) Exploring the role of tocotrienol-rich fraction (TRF) in ameliorating neuroinflammationInflammopharmacologyApril 20, 2026Jing Yi Tan, Thaarvena Retinasamy, Vanessa Lin Lin Lee, Ammu Kutty Radhakrishnan, Keng Yoon Yeong(cat. no. 410217) were obtained from Sigma-Aldrich, USA; whereas the Griess Reagent System (cat. no. G2930) was purchased from Promega, USA. Furthermore, the 1,1-diphenyl-2-picryl hydrazyl (DPPH) (cat. no. 281689),

(11) Monophosphoryl lipid A boosts macrophage antimicrobial immunity by metabolically regulating source-specific ROS generationFrontiers in ImmunologyMarch 30, 2026Dan Hao, Benjamin D. Klein, Margaret A. McBride, Julia K. Bohannon, Naeem K. Patil, Xenia D. Davis, Mary A. Oliver, Mei Lin Ning Dye, Sara Weidenbach, Jamey D. Young, et al.levels in cell culture supernatants (1 ×106) were quantified using the Griess Reagent System (Promega, G2930) according to the manufacturer’s instructions. Absorbance was measured at 520 nm. Xanthine oxidase

(12) FSH Receptor Asn680Ser Polymorphism Modulates Intrafollicular Nitric Oxide Bioavailability and Ovarian Responsiveness During IVFInt J Mol SciMarch 6, 2026Charalampos Voros, Diamantis Athanasiou, Despoina Mavrogianni, Ntilay Soyhan, Georgia Panagou, Maria Sakellariou, Georgios Papadimas, Fotios Chatzinikolaou, Eleni Sivylla Bikouvaraki, Georgios Daskalakis, et al.indicating the availability of nitric oxide. We used a commercial Griess Reagent System (Promega, Catalogue #G2930) to quantify the total nitric oxide metabolites (NOx = NO3− + NO2−). Calibration curves were constructed

(13) Microplastic-Induced Macrophage Dysfunction Drives Lung Tumor Progression through Glutathione ImbalanceACS NanoFebruary 24, 2026Bora Kim, Koung-Min Park, Haerang Lee, Young-Min Hyunin the dark. Subsequently, 50 μL of N-1-naphthylethylenediamine dihydrochloride solution (Promega, G2930) was added to each well, and the cells were further incubated for 10 min under identical conditions.

(14) Combined Flexion, Torsion and Compression Drive Distinct Intervertebral Disc Failure Mechanisms Under Asymmetric, High-Cycle LoadingJOR SpineFebruary 11, 2026Amra Šećerović, Aapo Ristaniemi, Francesco Crivelli, Sarah Heub, Mauro Alini, Gilles Weder, Diane Ledroit, Stephen J. Ferguson, Sibylle Grad1,9‐dimethyl‐methylene blue assay. NO release was measured using the Griess Reagent System (Promega, USA; #G2930), and interleukin‐6 (IL‐6) and interleukin‐8 (IL‐8) releases were quantified using bovine‐specific ELISA

(15) Anti-Inflammatory Effect of Excretion-Secretion Products of Clinostomum marginatum (Digenea: Clinostomidae) and Its Effect over the Viability and Antioxidative Activity of a Mix of Lactobacillus and/or BifidobacteriumMicroorganismsFebruary 3, 2026María Angélica Gutiérrez-Nava, Raquel González-Vázquez, Miguel Ángel Mosqueda-Cabrera, Daniela Reyna-González, Felipe Mendoza-Pérez, Eduardo Zúñiga-León, Leovigildo Mateos-Sánchez, Pedro A. Reyes-Castillo, Rosa González-Vázquez, María Guadalupe Córdova-Espinoza, et al.Quantification NO production in the culture supernatants was determined using the Griess reagent (PROMEGA G2930 kit, Promega, Madison, WI, USA) according to the manufacturer’s instructions. A standard curve was generated

(16) Pharmacological characterization and preclinical evaluation of 11h: a novel, brain-penetrant PDE4 inhibitor for neurological disordersFrontiers in PharmacologyJanuary 8, 2026Jacob Lackovic, Sougata Dey, Sara Jane Ward, Nigel H. Greig, David Tweedie, Nassim Beiranvand, Uksha Saini, Dev Chatterjee, Atul VaradhacharyNitrite levels were assessed by the use of the Griess Reagent System (Promega, Madison, WI, USA, Cat # G2930) or the Nitrate/Nitrite Fluorometric Assay Kit, (Abnova, Cat # KA1344). Statistical analysis was performed

(17) Macrophage Subtypes Distinctly Impact Intervertebral Disc Inflammation and Extracellular Matrix CompositionJOR SpineDecember 12, 2025Lauren E. Lisiewski, Leonardo Campos, Timothy D. Jacobsen, Min Kyu M. Kim, Nadeen O. Chahinemeasuring nitric oxide (NO) release into the media using the Griess Reagent System (Promega, Cat#. G2930). All NO release results are presented as cumulative release over the entirety of the culture period

(18) Synergistic Anticancer Activity of Fucoidan from Lessonia trabeculata Combined with Chemotherapeutic Agents in 4T1 Breast SpheroidsMarine DrugsNovember 26, 2025Rosa María Condori Macuri, Libertad Alzamora-Gonzales, Erasmo Honorio Colona-Vallejos, Raisa Teresa Cruz Riquelme, Laura Inés Pecho Chávez, Jherson Oscar Cisneros Gutierrez, Victor Alonso Montejo AnlasFrance, cat# 15290018), Griess Reagent System was purchased from Promega (Fitchburg, WI, USA, cat# G2930), and Ketamine/xylacine Injectable Solution for veterinary use was purchased from Ket-A-Xyl® (Lima,

(19) Host Translational Control by Stress Granules Promotes Mycobacterium tuberculosis PathogenesisMedComm (2020)November 10, 2025Jaewhan Kim, Sang‐Hun Son, Ji‐Ae Choi, Junghwan Lee, Seoyeon Jo, Soo‐Na Cho, Doan Tam Nguyen, Doyi Son, Kee K. Kim, Chang‐Hwa Songbreakdown product of NO, following the manufacturer's instructions for the Griess Reagent System (Promega #G2930). BMDM culture media were filtered and added to a 96‐well plate in triplicate. A standard solution was

(20) Nitric oxide regulates cytochrome P450 2D6 and 3A4 activity via concentration-dependent modulation of heme loadingJournal of Biological ChemistryNovember 1, 2025Priya Das Das Sinha, Sidra Islam, Pranjal Biswas, Dennis J. Stuehrwere collected, and nitrite accumulation in this medium was measured using the Griess reagent system (G2930; Promega). The plates were then harvested using cold lysis buffer (40 mM EPPS, 150 mM NaCl, 10% glycerol,

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(1)

12/10/2020Fast and Sensitive Nitric Oxide Assay Pauline MorinThe levels of nitric oxide in cell culture supernatants (LPS-inflamed RAW267.4) were measured using the Griess Reagent Assay. The test was fast to perfom, the instructions were easy and the test very ... Read Review
(1)

2/25/2015Using Griess Reagent System to measure Nitrite release from BV-2 cells Rebecca WilshusenThe goal of my research is to stimulate BV-2 cells (microglial cell line) with N-4YSyn (tail end of the antigenic protein found in Parkinson's disease) and then evaluate the BV-2 cells for increased ... Read Review

Griess Reagent System

Griess Reagent System from Promega

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Griess Reagent System from Promega

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