Fig 1: Functionalization of Pericardium With APX to Reduce Thrombosis(A) Molecular cargo dip-coating functionalization step. (B) Scanning electron microscopy images using back-scattered electron detector of gold-tagged APX (bright white dots) functionalized onto PPN-coated pericardium. (C) Whole blood clotting assay (scale bar represents 5 mm). (D) Quantification of blood clot weight. (Data presented as mean ± SEM compared using Student t test, 3 per group). (E) Scanning electron microscopy images of platelet attachment and aggregation on pericardium after 1-hour incubation. Platelets are false colored in orange (scale bar represents 4 μm). (F) PF4 enzyme-linked immunosorbent assay results after whole blood clotting assay. (Data presented as mean ± SEM compared using Student t test, 3 per group). Statistical significance is indicated by ∗P < 0.05, ∗∗P < 0.01. APX = apixaban; PF4 = platelet factor 4; Abbreviations as in Figure 1.