SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) 9003S from Cell Signaling Technology

Product Specs
ItemSimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads)
CompanyCell Signaling Technology
Price $563.00 Inquire
Catalog Number9003S
Quantity1 Kit
Research AreaChromatin Regulation / Nuclear Function
ApplicationsChIP, ChIP-seq

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) The Cyclin C-CDK8/19 Mediator kinase module controls PRCC-TFE3 driven senescence in renal epithelium and tumorigenesis in TFE3-RCCNeoplasiaMay 1, 2026Shoichiro Kuroda, Shintaro Funasaki, Hidekazu Nishizawa, Laura S. Schmidt, Ryoma Kurahashi, Takaaki Ito, Yuichiro Arima, Miwa Tanaka, Atsuya Kitada, Amy M. James, et al.manufacturer’s instructions using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003S), as previously described [12]. The following antibodies were used for immunoprecipitation: anti-Cyclin

(2) Anti-SIA-cIgG enhances chemotherapy effectiveness through PTPN13-regulated tumor stemness in head and neck squamous cell carcinomaJournal of Translational Internal MedicineApril 28, 2026Luming Wang, Yangyang Xia, Chenshu Liu, Xiaofeng Shan, Yi Sui, Shang Xie, Zhigang CaiS3. Chromatin immunoprecipitation Chromatin immunoprecipitation (ChIP) assay was performed using ChIP Kit (#9003, Cell Signaling Technology). According to the manufacturer’s protocol, cells were cross-linked with

(3) NFIB Regulates Chemoresistance in Small Cell Lung Cancer by Suppressing Notch Signaling ActivityCancer Rep (Hoboken)April 19, 2026Weixin Qin, Ziyan Wang, Shuzhe Deng, Huilei Qiu, Hongxue Meng, Jingshu GengAnalysis Chromatin immunoprecipitation was performed using the SimpleChIP Enzymatic Chromatin IP Kit (CST, #9003) according to the manufacturer's protocol with modifications [22]. Briefly, cells were crosslinked with

(4) Targeting the PPAR-γ/OPTN axis to inhibit apoptosis in steroid-induced glaucomaiScienceApril 17, 2026Xin Liang, Ning Li, Qiaoyun Gong, Haiyan WangAnnexin V-APC/PI Apoptosis Kit,Cat#Elabscience,Cat#E-CK-A217 SimpleChIP® Enzymatic Chromatin IP Kit,Cell Signaling Technology,Cat#9003 dual-luciferase Reporter Assay System,Promega,Cat#E1910 ,, Deposited data,Deposited data,Deposited

(5) Loss of Ezh2 Promotes M2-Like Macrophage Polarization in Hepatocellular CarcinomaiScienceApril 17, 2026Kittin Weerasopon, Atsadang Boonmee, Patipark Kueanjinda, Benjawan Wongprom, Thitiporn Pattarakankul, Thadaphong Sukdee, Tanapat Palagacollected and subjected to ChIP. ChIP was carried out using SimpleChIP Enzymatic Chromatin IP Kit (Cat. 9003, Cell Signaling Technology) following the manufacturer’s instruction. Briefly, BMDMs were crosslinked

(6) NF‑κB‑driven LUZP1 promotes metastasis and chemoresistance in head and neck squamous cell carcinomaOncol RepApril 7, 2026Chen-Yuan Lin, Ching-Yun Hsieh, Hsin-Chi Lan, Ching-Chan Lin, Tzu-Ting Chen, Wei-Chi Tseng, Yung-An Tsou, Wei-Chao Changgenomic loci in OECM-1 cells using the SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (cat. no. 9003; Cell Signaling Technology, Inc.). OECM-1 cells were cultured under standard conditions, stimulated

(7) A spinal USP7-Bach1 positive feedback loop drives NOX4-mediated ferroptosis in neuropathic painRedox BiolApril 6, 2026Bo Jiao, Shiyang Liu, Kaiwen Zhang, Wencui Zhang, Xueqin Cao, Xianwei Zhang, Jiaoli Sun, Hui Xu(ChIP-qPCR) ChIP-qPCR was performed in N2a cells using the SimpleChIP Enzymatic Chromatin IP Kit (9003, Cell Signaling Technology). Putative Bach1-binding motifs in the USP7 and NOX4 promoter regions were

(8) The HIF-2 transcription factor mediates resistance to ferroptosis in pancreatic cancerMol CellApril 2, 2026Maimon E. Hubbi, Catherine L. Wang, Yasir Suhail, Nadia L. Almasri, Jane Xie, Erin E. Hollander, Kshitiz, Alexander Muir, Ben Z. Stanger, Chi V. DangLipid Peroxidation (4-HNE) Assay Kit,Abcam,Cat# ab238538 Dual Luciferase Assay Kit,Promega,Cat# E1910 Chromatin IP Kit,Cell Signaling Tech,Cat# 9003 RNeasy Mini Kit,Qiagen,Cat# 74104 iScript cDNA Synthesis system,BioRad,Cat# 1708890 PureLink Genomic DNA Mini Kit

(9) Maternal obesity induces activator protein 1‐mediated inflammatory response to impair embryonic neurogenesisThe Journal of PhysiologyApril 1, 2026Li‐Wei Chen, Md Nazmul Hossain, Yao Gao, Zhongyun Kou, Sharmeen Islam, Xinrui Li, Chaeyoung Shin, Jeanene Marie de Avila, Mei‐Jun Zhu, Min Duimmunoprecipitation (ChIP) was performed using the SimpleChIP Plus Enzymatic Chromatin IP Kit (Magnetic Beads, CST 9003, Cell Signaling Technology, Danvers, MA, USA) following the manufacturer's protocol. Briefly 25 mg of

(10) Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interactionCell DiscoveryMarch 31, 2026Yi-yang Zhao, Yi-hui Wang, Zi-han Li, Dong-yuan Chang, Lin Nie, Ming-hui Zhao, Sydney Chi Wai Tang, Min ChenWestern blotting assay. ChIP assay The ChIP assay was performed via the SimpleChIP Enzymatic Chromatin IP Kit (9003S; Cell Signaling Technology). Briefly, TCMK-1 cells were harvested via a cell scraper after 20 min of

(11) An EGFR co-amplified lncRNA HELDR promotes glioblastoma malignancy through KAT7-driven gene programsNature Cell BiologyMarch 27, 2026Xiaozhou Yu, Xiao Song, Richard A. Schäfer, Qingshu Meng, Deanna Tiek, Runxin Wu, Qiu He, Maya Walker, Qi Cao, Rendong Yang, et al.sequential ChIP ChIP assays were performed by using the SimpleChIP Enzymatic Chromatin IP kit (CST, 9003) according to the manufacturer’s instructions. In brief, cells were crosslinked with 1% formaldehyde

(12) An HBV-Derived Peptide Poly6 as a Novel Candidate for Functional Cure Via IFN-I-Mediated Epigenetic Regulation of cccDNAJ Med VirolMarch 27, 2026Junghwa Jang, Dong Hyun Kim, Ziyun Kim, Eunseo Kim, Yu-Min Choi, Bum-Joon Kimexperiments. 2.4 HBV cccDNA Chip Assay ChIP assays were conducted using a ChIP Plus Enzymatic Chromatin IP kit (9003, CST, USA) according to the manufacturer's instruction. Briefly, cells were lysed after fixation, and

(13) CLOCK-catalyzed histone H3K37 glutarylation suppresses H3K36 trimethylation pathways in glioblastomaSci AdvMarch 27, 2026Yu Wang, Runxin Zhou, Xiao Zeng, Dingyuan Guo, Nan Li, Shuangli Li, Xu Zhang, Mingxuan Shi, Li Jiang, Mingzhu Fan, et al.and ChIP-seq assay ChIP was performed using a SimpleChIP Enzymatic Chromatin IP Kit (catalog no. #9003, Cell Signaling Technology). Chromatin prepared from cells in a 15-cm dish was used to determine total

(14) 18F-FDG-PET/CT-negative gastric cancer employs glutamine-based gluconeogenesis and fatty acid oxidation to support tumor growthCell Death & DiseaseMarch 26, 2026Jia Liu, Mingjie Xia, Zhexuan Zhao, Tian Gao, Yanzhao Qu, Qian Wang, Xiangdan Liu, Jianlan Du, Shunxin Han, Shiying Yang, et al.quench the reaction. After washing with ice-cold PBS, nuclei were isolated using the Chromatin IP Kit (#9003, Cell Signaling Technology) according to the manufacturer’s protocol. Chromatin was sheared using a

(15) SMARCA4 promotes lineage plasticity and enzalutamide resistance in prostate cancer by regulating PROX1 via H3K27 acetylationCell Death DiscoveryMarch 25, 2026Chenwei Wu, Mayao Luo, Chaojian Wu, Yi Yuan, Yadong Li, Yuanpeng Liao, Yifan Zhang, Xin Huang, Mengqi Wang, Shidong Lv, et al.preparation was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, 9003S, Massachusetts, USA). Initially, cells were fixed with formaldehyde. A total of 1 × 10⁵ cells were then

(16) The CHI3L1-neutrophil axis drives immune suppression and breast cancer metastatic disseminationJCI InsightMarch 23, 2026Tarek Taifour, Adéline Massé, Yu Gu, Virginie Sanguin-Gendreau, Dongmei Zuo, Bin Xiao, Emilie Solymoss, Yunyun Shen, Hailey Proud, Sherif Samer Attalla, et al.cm plates. ChIP was then performed using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling 9003) according to the manufacturer’s protocol. The following antibodies were used: STAT3 (1:50; Cell Signaling

(17) Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphomaCell Death & DiseaseMarch 23, 2026Wenqin Wang, Hui Zhou, Shuangxiong Tan, Dongmei Qin, Shuxuan Wang, Chunlan Xu, Xiangru Lei, Wenjuan Li, Liangjie Wang, Shuhui Fu, et al.the FoxO1 promoter region using the SimpleChIP Enzyme-Assisted Chromatin Immunoprecipitation Kit (#9003, Cell Signaling Technology). Chromatin was immunoprecipitated using an anti-PPARα antibody (#ab227074,

(18) Oncogenic GPRIN1 sustains proliferation and mitochondrial homeostasis via dual‑layer CDK1-PI3K/Akt signalling in gallbladder cancerCell Death & DiseaseMarch 21, 2026Chang Xu, Zijun Gong, Xiaojian Ni, Lingxi Nan, Wentao Sun, Houbao Liu, Xuanming Luo, Min Liusing the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, Cat# 9003) according to the manufacturer’s protocol. Briefly, cells were cross-linked with 1% formaldehyde, lysed,

(19) THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signalingiScienceMarch 20, 2026Huijuan Cheng, Dongfang Tang, Shousen Hu, Zizi Zhang, Weiwei Wang(1:20000, AC001, BioAb). ChIP ChIP assays were performed using the SimpleChIP Enzymatic ChIP Kit (9003, Cell Signaling Technologies). Chromatin from treated HPSCC cells was fixed, lysed, and sonicated. The

(20) S1P-S1PR1 signaling impairs CD8+ T cell metabolism and effector function in tumorsEMBO ReportsMarch 19, 2026Debashree Basak, Puspendu Ghosh, Anupam Gautam, Ishita Sarkar, Arpita Bhoumik, Soham Chowdhury, Shaun Mahanti, Anwesha Mandal, Rajeswari Chakraborty, Anwesha Kar, et al.X,632629 GSK2656157,Cayman,17372 SB203580,Cayman,13067 W146,Tocris,3602 ATP assay kit,Sigma,213-579-1 CHIP Kit,Cell Signaling Technology,9003 S1P,Sigma,S9666 Lonza electroporation buffer P3 kit,Lonza,Catno. V4XP-3032 Software/algorithm,Source,Link FlowJo

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SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads)

SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) from Cell Signaling Technology

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Cell Signaling Technology

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SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) from Cell Signaling Technology

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