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Assay Kits
A Guide to Choosing the Right ELISA Kit
ELISA Kit
Alpha-Klotho (Soluble) ELISA
Alpha-Klotho (Soluble) ELISA from IBL- America
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Citations:
(19)
Supplier Page
Supplier Page from
IBL- America for
Alpha-Klotho (Soluble) ELISA
Description
Solid phase sandwich ELISA for the determination of human soluble α-Klotho in serum and EDTA-plasma. For research use only, not for use in diagnostic procedures
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Alpha-Klotho (Soluble) ELISA
Company
IBL- America
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
27998
Detection Range
0 / 93.75 - 6000 pg/mL
Sensitivity
6.15 pg/mL
Volume
100 µL of properly diluted unknown / determination
Sample Type
Serum, plasma (EDTA, Heparin, Citrate), urine and cell culture supernatant
Regulatory Status
For research use only, not for use in diagnostic procedures
Applications
ELISA Kit
Quantity
96 Determinations
Reactivity
Human
Assay Time
1 hour incubation (RT) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
Storage
2 - 8 °C
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IBL- America
8201 Central Ave NE Suite P
Minneapolis
,
Minnesota
55432
United States
Phone
: 888-523-1246
763-780-2988
Company Profile
Website:
http://www.ibl-america.com/
Citations (19)
(1)
Relationship between klotho and physical function in healthy aging
Scientific Reports
November 30, 2023
Eliott Arroyo, Cecilia A. Leber, Heather N. Burney, Gayatri Narayanan, Ranjani Moorthi, Keith G. Avin, Stuart J. Warden, Sharon M. Moe, Kenneth Lim
serum using a well-validated solid-phase sandwich
enzyme-linked immunosorbent assay
(
ELISA
) (Cat. No.
27998
,
Immuno-Biological Laboratories
, Inc., Minneapolis, MN, USA)27. To eliminate inter-
assay
variance, all
(2)
A Combined Biomarker That Includes Plasma Fibroblast Growth Factor 23, Erythropoietin, and Klotho Predicts Short- and Long-Term Morbimortality and Development of Chronic Kidney Disease in Critical Care Patients with Sepsis: A Prospective Cohort
Biomolecules
October 3, 2023
Luis Toro, Verónica Rojas, Carolina Conejeros, Patricia Ayala, Alfredo Parra-Lucares, Francisca Ahumada, Paula Almeida, María Fernanda Silva, Karin Bravo, Catalina Pumarino,
et al.
FGF23: 60-6600, Quidel Immutopics, San Diego, CA;
EPO
:
DEP00
,
R&D Systems
, Minneapolis, MN. Klotho:
27998
,
IBL-America
, Minneapolis, MN, USA) according to manufacturer’s specifications. The inter-
assay
coefficient
(3)
Birth size and the serum level of biological age markers in men
Scientific Reports
August 30, 2023
Agnieszka Żelaźniewicz, Judyta Nowak-Kornicka, Bogusław Pawłowski
level was measured with
enzyme-linked immunosorbent assay
(
ELISA
) using commercial kits (
IBL
® Code no
27998
; with inter-
assay
and intra-
assay
coefficient of variation less than 11.4% and less than 3.5% respectively
(4)
Serum α-Klotho associated with oral health among a nationally representative sample of US adults
Front Endocrinol (Lausanne)
September 20, 2022
Guo-Qiang Chen, Yao Duan, Jin-Feng Wang, Ying Lian, Xiu-Li Yin
protocol, serum α-klotho was assayed using a solid-phase sandwich
enzyme-linked immunosorbent assay
(
ELISA
27998
,
IBL
International, Japan). This
assay
is reported to have a
sensitivity
of 6 pg/mL. All sample analyses
(5)
Serum α-KL, a potential early marker of diabetes complications in youth with T1D, is regulated by miRNA 192
Front Endocrinol (Lausanne)
August 5, 2022
Zhenwei Gong, Pedro A. Pagán Banchs, Ye Liu, Haoyi Fu, Vincent C. Arena, Erick Forno, Ingrid Libman, Jacqueline Ho, Radhika Muzumdar
Number: PRO15100286). Serum KL level Serum α-KL level was determined using ELISA kit from IBL (Cat#
27998
,
Immuno-Biological
Labs, Japan) according to the manufacturer’s instructions. The coefficient of variability
(6)
Gender-specific soluble α-klotho levels as marker of GH deficiency in children: a case–control study
Journal of Endocrinological Investigation
June 1, 2022
V. Guarnotta, G. Pizzolanti, R. Petrancosta, S. Radellini, C. Baiamonte, C. Giordano
analyzed using a commercial solid phase sandwich
ELISA
(
Enzyme-linked Immunosorbent Assay
)
assay
kit (cat.
27998
,
Immuno-Biological
Laboratoires Co., Fujioka-Shi, Japan). The normal measurement range was 93.75–6000
(7)
Orally-active, clinically-translatable senolytics restore α-Klotho in mice and humans
EBioMedicine
March 1, 2022
Zhu Y, Prata LGPL, Gerdes EOW, Netto JME, Pirtskhalava T, Giorgadze N, Tripathi U, Inman CL, Johnson KO, Xue A,
et al
.
diluted
mouse
urine (
IBL America
, #
27601
). Human urine α-Klotho was assayed by
ELISA
(
IBL America
, #
27998
, RRID:AB_2750859). Urine α-Klotho measurements are expressed as a function of urine volume, creatinine
(8)
S-Klotho level and physiological markers of cardiometabolic risk in healthy adult men
Aging (Albany NY)
January 31, 2022
Agnieszka Żelaźniewicz, Judyta Nowak-Kornicka, Bogusław Pawłowski
level was measured with
enzyme-linked immunosorbent assay
(
ELISA
) using commercial kits (
IBL
® Code no
27998
; with inter-
assay
and intra-
assay
coefficient of variation less than 11.4% and less than 3,5% respectively
(9)
Epigenetic and senescence markers indicate an accelerated ageing-like state in women with preeclamptic pregnancies
EBioMedicine
August 11, 2021
Sonja Suvakov et al.
concentrations were measured using the Alpha Klotho Human Soluble ELISA Assay kit (
IBL-America. Inc
, Cat#
27998
). α-Klotho was normalized to urinary creatinine concentration measured using a creatinine
assay
kit
(10)
Assessment of Clinical Utility of Assaying FGF-23, Klotho Protein, Osteocalcin, NTX, and Sclerostin in Patients with Primary Hyperparathyroidism
Journal of Clinical Medicine
July 13, 2021
Monika Sykała et al.
soluble α-Klotho
assay
kit—IBL (
Immuno-Biological Laboratories
Co. (Minneapolis, MN, USA); code no.
27998
); minimum detectable concentration: 6.15 pg/mL; CV = 2.7%.Osteocalcin: enzyme-linked immunosorbert
assay
(11)
A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma
PloS one
January 1, 2021
Jadhav S, Tripathi S, Chandrekar A, Waikar SS, Hsiao LL.
failed to detect rsKL. We next tested the most commonly used commercially available ELISA kit from IBL (#
27998
,
Immuno-biological
PLOS ONE | https://doi.org/10.1371/journal.pone.0245614 January 22, 2021 3/9 PLOS
(12)
Fibroblast growth factor 23 decreases PDE4 expression in heart increasing the risk of cardiac arrhythmia; Klotho opposes these effects
Basic Research in Cardiology
September 1, 2020
Marta Lindner, Hind Mehel, Amandine David, Christine Leroy, Martine Burtin, Gérard Friedlander, Fabiola Terzi, Delphine Mika, Rodolphe Fischmeister, Dominique Prié
concentration of sKlotho was determined with the IBL Elisa kit (
Human
soluble α-Klotho
Assay
Kit code No.
27998
–
IBL
, Japan). 6 cAMP measurements by
FRET
. ARVMs were infected with an
adenovirus
encoding the
(13)
Effect of kidney donation on bone mineral metabolism
PLOS ONE
July 7, 2020
Thomas F. Hiemstra et al.
of human FGF-23. Serum α-Klotho levels were analyzed (
Immuno-Biological Laboratories
Co., Ltd., Cat #
27998
). 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D were analysed by automated chemiluminescent
immunoassay
(14)
Urinary Extracellular Vesicles Carrying Klotho Improve the Recovery of Renal Function in an Acute Tubular Injury Model
Molecular therapy : the journal of the American Society of Gene Therapy
February 5, 2020
Grange C, Papadimitriou E, Dimuccio V, Pastorino C, Molina J, O'Kelly R, Niedernhofer LJ, Robbins PD, Camussi G, Bussolati B.
quantification of Klotho levels within EVs was performed using an anti-
human
ELISA
(
Human
soluble a-Klotho code
27998
;
Immuno-Biological Laboratories
)73 according to the manufacturer’s protocol. In detail, three pools
(15)
FGF23-klotho axis as predictive factors of fractures in type 2 diabetics with early chronic kidney disease
Journal of Diabetes and its Complications
January 1, 2020
Ana Luísa Ribeiro, Filipa Mendes, Eduarda Carias, Fátima Rato, Nélio Santos, Pedro Leão Neves, Ana Paula Silva
(Cat.#60-6100 Immutopics Inc., San Clemente, CA, USA) and
Human
soluble αKlotho ELISA kit (Code No.
27998
,
IBL
– Immuno-Biological Laboratories Co., Ltd., Gunma, Japan), according to the manufacturer's instructions. The
(16)
Plasmatic Klotho and FGF23 Levels as Biomarkers of CKD-Associated Cardiac Disease in Type 2 Diabetic Patients
International Journal of Molecular Sciences
March 27, 2019
Ana Paula Silva et al.
Soluble α-Klotho serum levels were determined by
ELISA
using the
Human
soluble α-Klotho kit (Code no-
27998
,
IBL
– Immuno-Biologial Laboratories Co., Ltd., Gunma, Japan), by adapting the manufacturer’s instructions
(17)
Aldosterone induces renal fibrosis by promoting HDAC1 expression, deacetylating H3K9 and inhibiting klotho transcription
Molecular Medicine Reports
December 20, 2018
Lingyun Lai, Ping Cheng, Minhua Yan, Yong Gu, Jun Xue
was determined using an ELISA kits obtained from
Immuno‑Biological Laboratories
Co., Ltd. (cat. no.
27998
; Fujioka, Japan), according to the manufacturer's instructions. ∆ aldosterone was calculated using the
(18)
The Value of Older Donors' Klotho Level in Predicting Recipients' Short-Term Renal Function
Medical science monitor : international medical journal of experimental and clinical research
November 5, 2018
Deng G, Yang A, Wu J, Zhou J, Meng S, Zhu C, Wang J, Shen S, Ma J, Liu D.
of recipients were also calculated. Serum Klotho (
Immuno Biological Laboratories
Co., Tokyo, Japan,
27998
), intact FGF-23 (R&D Systems, Minneapolis, MN, USA,
DY2604-05
), and urinary
NGAL
(
R&D Systems
, Minneapolis,
(19)
Multiple sclerosis influences on the augmentation of serum Klotho concentration
Journal of the Neurological Sciences
March 15, 2016
Mona Ahmadi, Mohammad Sajad Emami Aleagha, Mohammad Hossein Harirchian, Reza Yarani, Farhad Tavakoli, Bahaadin Siroos
measured using a sandwich ELISA assay kit (
human
soluble αklotho
assay
kit,
IBL
Co., Japan, Code No.
27998
and Lot No. 1L-303). The
sensitivity
of kit was 6.15 pg/ml. The
assay
was performed according to the
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Alpha-Klotho (Soluble) ELISA from IBL- America
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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