CytoTox 96 Non-Radioactive Cytotoxicity Assay G1780 from Promega

Product Specs
ItemCytoTox 96 Non-Radioactive Cytotoxicity Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG1780
Detection TargetCytotoxicity
Quantity1,000 assays
MethodColorimetric
Sample Typeculture supernatants
Assay Time30 minutes
Molecular Weight~210kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Disruption of macrophage cell volume drives inflammatory responses and type I interferon signalingJ Cell BiolJune 1, 2026James R. Cook, Tara A. Gleeson, Sara Gago, Stuart M. Allan, Kevin N. Couper, Catherine B. Lawrence, David Brough, Jack P. GreenMerck). Cell death assays Cell death was assessed via a MTT assay kit (ab211091; Abcam), an LDH release kit (G1780; Promega), and a Caspase-Glo 3/7 kit (G8090; Promega) according to the manufacturer’s instructions.

(2) Aldh3a1-mediated detoxification of reactive aldehydes contributes to distinct muscle responses to amyotrophic lateral sclerosis progressionFree Radical Biology and MedicineJune 1, 2026Ang Li, Li Dong, Xuejun Li, Jianxun Yi, Jo-Yu Wu, Jianjie Ma, Jingsong Zhoulow serum medium before TUNEL assay (Biotium 30074) or LDH leakage-based cytotoxicity assay (Promega G1780). This is because apoptosis can take 12-24 hours to occur [50]. TUNEL assay Triplicate cultures of

(3) Hyperactivated glycolysis drives spatially patterned Kupffer cell depletion in MASLDElifeMay 26, 2026Jia He, Ran Li, Cheng Xie, Xiane Zhu, Keqin Wang, Zhao Shanblue. LDH activity in culture supernatants was measured using a commercial assay kit (Promega, Cat# G1780) according to the manufacturer’s instructions. Absorbance was then measured at 490 nm using a microplate

(4) Microfluidics-enabled proteomic profiling reveal iron-driven immune evasion by an antimicrobial-resistant pathogenCell Reports MethodsMay 18, 2026Chikim Nguyen, Chelsea Reitzel, Arjun Sukumaran, Kristina A. Ganzinger, Jennifer Geddes-McAlisterPromega CytoTox 96 Non-Radioactive Cytotoxicity Assay according to manufacturer instructions (Promega, G1780). Briefly, 50 μL of supernatant was collected and combined with 50 μL substrate. Max cell lysis was

(5) Inhibiting MDM2 enhances RIPK3-mediated necroptosis and synergizes with immune checkpoint blockade therapyiScienceMay 15, 2026Yingxin Wu, Hanyang Yu, Zongxu Zhang, Weihang Xiong, Zexian Zeng, Weili Liu, Hailin Tu, Xin Linlactate dehydrogenase (LDH)- based assays (CytoTox 96 Non-Radioactive Cytotoxicity Assay, Promega #G1780). L929, SW480 cells were seeded the day before at 3×104 per well while E.G7-OVA at 1×105 per well in

(6) Programmed death-ligand 1 / Lymphocyte-activation gene 3 bispecific immune checkpoint blocking nanocage exhibits potent antitumor activity beyond dual blockade of programmed death-ligand 1 and lymphocyte-activation gene 3Biomaterials ResearchMay 7, 2026Seok-Min Lee, Minseong Kim, Chanju Lee, Minah Lee, Hee Jung Yoon, Byungheon Lee, Eun Jung Park, Soyoun Kimevaluated using a lactate dehydrogenase (LDH) assay (CytoTox96 Nonradioactive Cytotoxicity Assay, Promega, G1780), according to the manufacturer’s instructions. For quantitative measurement of viable MC38 cells remaining

(7) An IL-1, IL-17, and IL-22 cytokine circuit controls vulvovaginal candidiasis independently of estrogenPLoS PathogMay 7, 2026Bianca M. Coleman, Melissa E. Cook, Md. Robin Khan, Amanda K. Vogel, Anthony J. Wells, Jian Miao, Shachi P. Vyas, Tiffany C. Taylor, Felix E.Y. Aggor, Nicole O. Ponde, et al.each analyte. LDH in VLF was measured using CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega, G1780). OD492 was determined using a BioTek Synergy 2 microplate reader. Samples were quantified against a

(8) Recapitulation of clinical and molecular hallmarks of lipid-induced hepatic insulin resistance in a zonated, vascularized human liver acinus microphysiological system during metabolic dysfunction-associated steatotic liver disease (MASLD) progressionBMC BiotechnologyApril 30, 2026Julio Aleman, Lawrence Vernetti, Mark E. Schurdak, Richard DeBiasio, Greg LaRocca, Vijay K. Yechoor, D. Lansing. Taylor, Andrew M. Stern, Mark T. Miedelrespectively. Urea (Stanbio Laboratory, cat. no. SB-0580–250) and lactate dehydrogenase (Promega, cat. no. G1780) were measured with no efflux dilution. Controls were run in duplicate, and only sample values inside

(9) C-type lectin domain family 5 member A-mediated activation of macrophages via a bispecific antibody enhances anti-HER2 therapyJournal for ImmunoTherapy of CancerApril 29, 2026Lei Hou, Dechuang Jiao, Hao Dai, Jingyang Zhang, Lina Wang, Xilong Gong, Cuihua Qi, Xuhui Guo, ZhenZhen Liucell cytotoxicity was assessed using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega, Cat# G1780). Target cells (BT474 or A20-HER2, 1×10⁴ cells/well) were seeded in 96-well plates and co-cultured with

(10) Cytocompatibility of PMMA and Titanium Boston Keratoprosthesis Backplates with Human Corneal FibroblastsBioengineering (Basel)April 29, 2026Antonio Esquivel Herrera, Liangju Kuang, Mark Krauthammer, Michael Bednar, Eleftherios I. Paschalis, Thomas H. Dohlman5, and 7 using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Madison, WI, USA; Cat. #G1780) and following the manufacturer’s protocol. At each time point, 50 µL of culture supernatant was collected

(11) Optimizing Photobiomodulation for Smooth Muscle Differentiation of Adipose-Derived Stem Cells Using Retinoic Acid and TGFβ in a Two-Dimensional ModelCellsApril 27, 2026Christevie Mbuyu, Heidi Abrahamse, Anine CrousCytotoxicity Lactate Dehydrogenase (LDH) Cytotoxicity Assay The Cytotox 96® (Promega, Madison, WI, USA; G1780), non-radioactive cytotoxicity assay, is important for the measurement of LDH, an essential enzyme for

(12) NEK7 Cys298 is important for NLRP3 inflammasome and targetable by dimethyl fumarateCell Chem BiolApril 21, 2026Yandong Zhang, Helena Lindner, Brian So, Zirong Chen, Haoyang Li, Jiashu Xu, Jiahe Wang, Jie Xu, Alexandru M. Petre, Mark D. Distefano, et al.and culture media were collected for immunoblotting/qPCR analysis, LDH release assay (Promega, Cat# G1780) and IL-1β ELISA (R&D Systems, Cat#DLB50), respectively. For immunoblotting, anti-Casp1-P20 (Adipogen,

(13) Dual inhibition of TRIP13 and Aurora A induces mitotic DNA damage and concurrent pyroptotic-apoptotic cell death in Rb-deficient cancer cellsClin Cancer ResApril 21, 2026Lacin Yapindi, Soma Ghosh, Li Shen, Lixia Diao, Jing Wang, Faye M. Johnsonassay LDH release was measured using a CytoTox 96 Non-Radioactive Cytotoxic Assay Kit (Promega; cat. no. G1780) according to the manufacturer’s instructions. Briefly, cells were seeded at a density of 5000cells/well

(14) A distinct TCR+CD19+ population in the peritoneal cavity: B1a cells as precursors of extrathymic T cellsiScienceApril 17, 2026Su-Min Yee, Jeong-Eun Seon, Jae-Eun Seo, Hae-Jin Seo, Ja-Hun Seo, Seo-Hee Moon, Hyun-Su Koo, Ha-Rim Choi, Su-Man Kim, Hyung-Sik KangMouse IL-17 Quantikine ELISA Kit,R&D Systems,Cat#M1700-1 CytoTox 96 Non-Radioactive Cytotoxicity Assay,Promega,Cat#G1780 eBioscience™ Foxp3/Transcription Factor Staining Buffer Set,Thermo Fisher Scientific,Cat#00-5523-00 Nuclear Extraction Kit

(15) Synergetic attenuated inflammatory response and increased apoptosis confer high pathogenicity of Acinetobacter baumanniiVirulenceApril 17, 2026Lele Liu, Luyu Yang, Qingqing Xie, Xiaopeng Qi, Hongwei Pan, Hong-Peng Dong, Xuexing Zheng, Tao Xucontrol, LDH activity was assessed with the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780) according to the manufacturer’s instructions. Three independent experimental replicates were performed,

(16) FOXE1 promotes the progression of pulp inflammation by activating PANoptosis in dental pulp cellsiScienceApril 17, 2026Fan Gu, Ruiqi Li, Delan Huang, Linlin Peng, Tingting Huan, Kaili Ye, Zhuan Bian, Wei Yinassay The release of LDH by DPCs was measured using a CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (#G1780, Promega). Briefly, DPCs were seeded in 96-well plates and then infected with S.m., F.n. or a combination

(17) Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid modelThe EMBO JournalApril 16, 2026Antonella F M Dost, Katarína Balážová, Carla Pou Casellas, Lisanne M van Rooijen, Wisse Epskamp, Gijs J F van Son, Willine J van de Wetering, Carmen Lopez-Iglesias, Harry Begthel, Peter J Peters, et al.where applicable, were added to the media on day 11 of culture, and the cytotoxicity assay (Promega #G1780) was performed on day 14 according to the manufacturer’s protocol. Briefly, 50 ul of media was transferred

(18) TLR2 activation potentiates P-glycoprotein-mediated methotrexate efflux and enhances cytotoxicity of human NK cells against acute lymphoblastic leukemiaFront ImmunolApril 15, 2026Pablo Álvarez-Carrasco, Paola Basurto-Olvera, Elva Jiménez-Hernández, Aurora Medina-Sanson, Carmen Maldonado-BernalLDH release assay (CytoTox 96® Non-Radioactive Cytotoxicity Assay; Promega, Madison, WI, USA; Cat: G1780), appropriate controls were included for each donor. Following incubation, samples were centrifuged

(19) Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxinInfection and ImmunityApril 15, 2026Désirée Schaaf, Muriel Dresen, Yenehiwot Berhanu Weldearegay, Jeannine Biermann, Susan L. Brockmeier, Wolfgang Baumgärtner, Michael Jarek, Peter Valentin-Weigandlactate dehydrogenase (LDH) using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega, Cat. No. G1780) as previously described (8). LDH release of infected PCLS/ALI cultures was normalized to uninfected

(20) An enhanced-permeability amphiphilic nanogel loaded with sulforaphane for synergistic anti-oxidative and anti-ferroptotic therapy of corneal alkali burnJournal of Translational MedicineApril 14, 2026Chengcheng Yang, Yuxi Huang, Yichi Zhang, Yubo Zhang, Wenting Luo, Xiaomei Ke, Songying Pi, Hui Chen, Shushan ZhangSigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). The CytoTox 96® Non-Radioactive Cytotoxicity Assay (cat. G1780) and CellTiter 96 Aqueous One Solution Cell Proliferation assay (cat. G3580) were supplied by Promega Corporation

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(1)

5/15/2019Easy-to-Use Cytotoxicity Assay from Promega Kiara FreitagVery good kit for fast quantification of LDH release of cultured cells!
(1)

1/30/2017Easy Assay To Measure LDH Levels In Cell Supernatant Tara VanderweydeThis assay is super easy and can easily be coupled to other assays, as you only take the supernatant from the cells to look at released LDH. It is measured with a 30-minute coupled enzymatic assay, ... Read Review

CytoTox 96® Non-Radioactive Cytotoxicity Assay From Promega
The Promega CytoTox 96® Non-Radioactive Cytotoxicity Assay is an alternative assay format to ... Read More
Promega's CytoTox 96 NonRadioactive Cytotoxicity Assay
The CytoTox 96 Non-Radioactive Cytotoxicity Assay from Promega is an easy-to-use, 2 step, ... Read More

CytoTox 96 Non-Radioactive Cytotoxicity Assay

CytoTox 96 Non-Radioactive Cytotoxicity Assay from Promega

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Promega

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CytoTox 96® Non-Radioactive Cytotoxicity Assay From Promega

Promega's CytoTox 96 NonRadioactive Cytotoxicity Assay

CytoTox 96 Non-Radioactive Cytotoxicity Assay from Promega

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