Fig 1: Over-expression of ZMAT1 promotes apoptosis in Pancreatic Ductal Adenocarcinoma (PDAC) cells. A Flow cytometry showed that over-expression of ZMAT1 increased the percentage of apoptotic SW1990 cells. B-C Flow cytometry showed that ZMAT1 deletion reduced the percentage of apoptotic SW1990 cells. D-F Western blot showed over-expression of ZMAT1 up-regulated BAD, BAX, Cleaved Caspase 3 and declined Bcl-2 in SW1990 cells (D), whereas ZMAT1 knockdown reduced BAD, BAX, Cleaved Caspase 3 and increased Bcl-2 in BXPC-3 (F) and Capan-2 cells (G). All * P-value < 0.05, ** P-value < 0.01, *** P-value < 0.001. P-values were assessed using two-tailed t-tests in C-E. All figures represent mean ± SD from three independent experiments