Senescence beta-Galactosidase Staining Kit 9860S from Cell Signaling Technology

Product Specs
ItemSenescence beta-Galactosidase Staining Kit
CompanyCell Signaling Technology
Price $283.00 Inquire
Catalog Number9860S
Quantity1 Kit
Research AreaCell Cycle / Checkpoint Control
Detection Targetbeta-galactosidase bacteria
NCBI Gene AliasesECK0341
ApplicationsFUNC

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Age-related GSS promoter methylation in BMSCs drives osteoporosis and the reversal by targeted GSH deliveryBioactive MaterialsJune 1, 2026Pan Li, Zhuowen Liang, Xianyan Zeng, Runbo Lei, Shuo Guo, Zhao Zhang, Guangwei Zhang, Jianxiong Li, Anhui Qin, Mi Qu, et al.rehydration, sections were incubated in SA-β-gal staining solution (pH 6.0; Cell Signaling Technology #9860) at 37 °C for 16 h in a humidified chamber protected from light. Following three washes in PBS, nuclei

(2) Sulfated polysaccharide prevents senescent adipocyte-driven osteonecrosis by stem cell fate reprogrammingBioactive MaterialsJune 1, 2026Shuang Zhang, Shunshu Deng, Kai Dai, Yang Liu, Jing Wang, Changsheng Liutreatment, frozen femoral sections were stained with a SA-β-Gal staining kit (Cell Signaling Technology, 9860) according to the manufacturer's protocol. Sections were incubated in the staining solution at 37 °C

(3) THEMIS attenuates MASH by suppressing disease-associated hepatocyte induction and hepatocyte senescence in miceJ Clin InvestMay 1, 2026Xiaoxue Qiu, You Lu, Yuwei Tang, Linkang Zhou, Yu-Tung Lee, Ziyi Meng, Zhimin Chen, Fnu Pradeepa, Lanuza A.P. Faccioli, Zhiping Hu, et al.cell culture were evaluated by SA-β-GAL staining using a commercial kit (Cell Signaling Technology, 9860), following the manufacturer’s instructions. Images were captured using microscopy (Thermo Fisher, EVOS

(4) Extracellular vesicles secreted by adipose-derived stem cells alleviate dedifferentiation and senescence in monolayer-expanded chondrocytes by suppressing interleukin-1β-induced MAPK signalingCell TransplantApril 25, 2026Shun-Cheng Wu, Ling-Hua Chang, Che-Wei Wu, Chung-Hwan Chen, Je-Ken Chang, Mei-Ling Hostaining Senescence was assessed using a senescence-associated β-galactosidase (SA-β-gal) staining kit (cat. no. 9860; Cell Signaling Technology, Danvers, MA, USA) following the manufacturer’s instructions. Briefly, chondrocytes

(5) Histone Deacetylase 9 Gene Deletion Ameliorates Aging-Related Adipose Tissue Senescence and Mitochondrial Dysfunction in MiceAging CellApril 25, 2026Brandee Goo, Samah Ahmadieh, Praneet Veerapaneni, Hong Shi, David S. Kim, Mourad Ogbi, Ronnie Chouhaita, Nicole Cyriac, Stephen Cave, Lingling Liu, et al.cultured preadipocytes were stained for SABG using the senescence β‐galactosidase staining kit (CST #9860) per manufacturer's instructions. Briefly, samples were washed with phosphate buffered saline (PBS)

(6) An In Vitro Model of Macrophage SenescenceCurr ProtocApril 22, 2026Grasiela Torres, Utkarsh Tripathi, Ivan Salladay-Perez, Itzetl Avila, Anthony J. Covarrubiassterile and cold (4°C) (Current Protocols, 2006)SA‐β‐galactosidase staining kit (Cell Signaling, cat. no. 9860) ParafilmIncubator set to 37°C (without CO2)Brightfield microscope 1Wash cells once with sterile

(7) New isohemigossypol related conjugates and caffeoylated oleanane triterpenoids from Hibiscus syriacus roots with anti-senescence modulating activityRSC AdvApril 20, 2026Hyo-Moon Cho, Minh-Thi-Tuyet Le, Ji-Hye Shin, Van-Hieu Mai, Seri Choi, Jin-Pyo An, Yun-Sil Lee, Won-Keun Ohthe test compounds for another 48 h. SA-β-gal activity was detected using a commercial staining kit (#9860, Cell Signaling Technology, Danvers, MA, USA) according to the manufacturer's instructions. The stained

(8) p21+TREM2+ senescent macrophages fuel inflammaging and metabolic dysfunction-associated steatotic liver diseaseNat AgingApril 16, 2026Ivan A. Salladay-Perez, Itzetl Avila, Lizeth Estrada, Andreea C. Alexandru, Cristian Ponce, Anika Dhingra, Grasiela Torres, Christina Y. Deng, Ronak Hegde, Julia Gensheimer, et al.senescence detection kit as per the manufacturer’s instructions (Cell Signaling Technologies, cat. no. 9860S or Biovision Senescence Detection Kit, cat. no. K320-250). Shortly, cultured macrophages were fixed,

(9) Cell Type-Specific Expression of p16, p21, and p53 Reveals Age-Dependent Glial Senescence in the AppNL-G-F Mouse Model of Alzheimer's DiseaseAging CellApril 14, 2026Eileen Mac Sweeney, Giulia Abate, Bjorn R. V. Bakker, Andrea Mastinu, Ylenia Lai, Daniela Uberti, Per Nilsson, Simone TambaroSA‐β‐gal staining was performed according to the protocol of the Senescence‐β‐Galactosidase Staining Kit (#9860, Cell Signaling Technology, Danvers, MA, USA). For the double staining with Iba1, tissues were further

(10) Dasatinib and quercetin senolytic treatment delays early onset intervertebral disc degeneration in SM/J miceBone ResApril 14, 2026Emanuel J. Novais, Olivia K. Ottone, Sanjana Jagannath, Esther Jesutofunmi Akande, Ruteja A. Barve, Makarand V. Risbudwith 1 mg/ml X-Gal solution following the manufacturer’s instructions (Cell Signaling Technology, #9860). After overnight incubation at 37 °C without CO₂, cells were washed and examined under a bright-field

(11) Pseudo-senescence induced by palbociclib does not sensitise pleural mesothelioma cells to combinations with senolyticsCell Death DisApril 10, 2026Iswarya Sreeram, Sílvia Plans-Marin, Mabel Cruz-Rodríguez, Elisabet Aliagas, Didac Palau-Gallinat, Cristina Muñoz-Pinedo, Ernest Nadalactivity Senescence was measured by the procedure provided in the Senescence β-Galactosidase Staining Kit (#9860 Cell Signalling Technologies). For live senescent cell sorting, the Cellular Senescence Live Cell Analysis Assay

(12) Behavior and Musculoskeletal Effects of Chronic D-Galactose Treatment in Mice: Role of Heme Oxygenase-1BiomoleculesApril 8, 2026Sally Wahba, Olufunto O. Badmus, Andrew R. Wasson, Elshymaa A. Abdel-Hakeem, Merhan Mamdouh Ragy, Hanaa Mohamad Ibrahim, Daniela Rüedi-Bettschen, David E. Stecinstructions (Senescence β-Galactosidase Staining Kit, Cell Signaling Technology, Danvers, MA, USA, #9860). Brain sections were washed once with 1× PBS (200 µL/section), then 200 µL of 1× fixative solution

(13) Gastrodia elata-Derived Parishin Extracts Attenuate Aging by Modulating Oxidative Stress, Inflammation, Apoptosis, and MetabolismFood Sci NutrApril 3, 2026Zhu Li, Yue Chen, Huihuang Shi, Peng Tang, Xiangui ZhangStaining Cells were fixed and stained using a β‐galactosidase (SA‐β‐gal) staining kit (Cell Signaling, 9860) according to a previously described protocol with minor modifications (Kang et al. 2024). Briefly,

(14) ER-localized ceramide accumulation contributes to replicative senescenceCell Chemical BiologyMarch 30, 2026Shweta Chitkara, Mengru Li, Natasha Gozali, Andrey Kuzmin, Artem Pliss, Paras Prasad, Yasemin Sancak, G. Ekin Atilla-Gokcumenkit,Senescence-associated β-galactosidase staining kit,Cell Signaling Technology,Cell Signaling Technology,Cat#9860,Cat#9860 E.Z.N.A. Plasmid DNA Mini Kit,E.Z.N.A. Plasmid DNA Mini Kit,Omega Bio-Tek,Omega Bio-Tek,Cat#D6945-01,Cat#D6945-01 iScript

(15) Replicative senescence induction in single cells is not predicted by telomere length, dysfunction, or oxidationiScienceMarch 20, 2026Victor Passanisi, Sabrina L. Spencercommercial assays,Critical commercial assays ,, Senescence-Associated β-Gal Staining Kit,CST,Cat# 9860 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Hs68,ATCC,CRL−1635 AG06234,Coriell

(16) PPAR-γ suppresses macrophage senescence and allergic airway inflammation through controlling lipid metabolic pathwaysEBioMedicineMarch 20, 2026Wenjing Gu, Rongjun Wan, Zhifeng Chen, Wenshen Wang, Shaobing Xie, Chuangli Hao, Guangshu Liu, Mei Wan, Peisong Gaosenescence-associated β-galactosidase (SA-β-Gal) using a commercial kit (Cell Signalling Technology, #9860S) following the manufacturer's instructions. Immunofluorescence staining Immunofluorescence staining

(17) Resveratrol Mitigates Age-Associated Cognitive Decline via Inhibition of cGAS-STING-Mediated Microglial SenescenceCellsMarch 16, 2026Xinxin Duan, Jiahui Cheng, Jiayao Wang, Wen Chen, Zhi Ruan-frozen tissue sections according to established protocols with minor modifications (Cell Signaling Technology, #9860). Briefly, 30 μm cryosections were thawed and washed with PBS. After three washes with PBS, sections

(18) The REDD1–NF-κB–miRNAs–eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertensionNature CommunicationsMarch 12, 2026Yoon Kyung Choi, Dong-Keon Lee, Minsik Park, Junyoung Byeon, Woo-Young Nam, Thuy Linh Lai, Kyu Nam Kim, Okhwa Kim, Sungwoo Ryoo, Jeong-Hyung Lee, et al.and immunohistochemistry EC and aortic vessel senescence were assessed using the Senescence β-Galactosidase Staining Kit (#9860, Cell Signaling Technology, Danvers, MA, USA) according to the manufacturer’s protocol. Images of the

(19) Targeting oncogene-induced senescence in ETV6::RUNX1 pre-leukemic cellsCell Death DiscoveryMarch 11, 2026Denise Acunzo, Mayla Bertagna, Giulia Risca, Linda Beneforti, Silvia Bresolin, Stefania Galimberti, Isidro Sánchez-García, Andrea Biondi, Giovanni Cazzaniga, Chiara Palmilaser. Colorimetric SA β-GAL activity analysis We used the Senescence β-Galactosidase Staining Kit (#9860, Cell Signaling, Danvers, USA) to colorimetrically detect the SA β-Gal activity according to the manufacturer’s

(20) 2-Methoxystypandrone from Polygonum cuspidatum Rejuvenates Senescence by Reducing Mitochondrial ROSAntioxidants (Basel)March 11, 2026Jee Hee Yoon, Ye Hyang Kim, Minseon Kim, Eun Young Jeong, Yun Haeng Lee, Ji Ho Park, Yoo Jin Lee, So Hun Lee, Ha Yeon Kim, Hye Min Kang, et al.days at 4-day intervals with either 2-MS (4, 8, and 12 μM) or DMSO (0.01%). For SA-β-Gal staining (9860; Cell Signaling Technology, Beverly, MA, USA), the manufacturer’s instructions were adhered to. 2.9.

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2/18/2022Kit Detects Senescence-Associated Beta-Galactosidase in Fresh Gonadal and Subcutenous Fat of Chronologically Aged Mice Helena AiresThe Senescence ß-Galactosidase Staining Kit is designed to detect ß-galactosidase activity at pH 6, a known characteristic of senescent cells.
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9/11/2015Senescence ß-Galactosidase Staining Kit for Cells In Vitro A RWe used this SA-beta-gal staining kit for determining senescence in cultured mouse bone marrow derived MSCs. We were able to detect SA-beta gal positive cells after culturing them in MSC culture ... Read Review

Senescence beta-Galactosidase Staining Kit

Senescence beta-Galactosidase Staining Kit from Cell Signaling Technology

Cell Signaling Technology

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Senescence beta-Galactosidase Staining Kit from Cell Signaling Technology

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