Serine/Threonine Phosphatase Assay System V2460 from Promega

Product Specs
ItemSerine/Threonine Phosphatase Assay System
CompanyPromega
Price $299.00Supplier Page View Company Product Page
Catalog NumberV2460
Detection TargetSerine/Threonine Phosphatase
Detection Range100–4,000pmol of phosphate
MethodColorimetric
Quantity96 reactions

Specifications/Features

Test/Assays:
1 kit/96 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Are PP2Cs with intrinsically disordered regions CO2 sensors that control stomatal movements?Plant PhysiolMay 18, 2026Karnelia Paul, Mritunjay Kasera, Yohei Takahashi, Hyunhee Joo, Po-Kai Hsu, Julian I Schroederphosphatase activity of PP2C5(AP2C3) was measured using the Serine/Threonine Phosphatase Assay System (V2460, Promega) (Zhang et al. 2022). Reactions contained 5 µL of 100 µM Ser/Thr phosphopeptide (RRA(pT)VA)

(2) PPM1B utilizes a trinuclear metal architecture for phosphatase activitybioRxivMay 4, 2026Reece P. Stevens, Viktoriya Solodushko, Andrzej Wierzbicki, Thomas C. Rich, Mikael F. Alexeyev, Marlo K. Thompson, Madeline Stone, Camryn Hall, Althea deWeever, Sarah L. Sayner, et al.Phosphopeptide activity assays were performed with the Promega Serine/Threonine Phosphatase Assay System (V2460). A phosphopeptide derived from IKKβ(pS) (residues 175–184, QG(pS)LCT(pS)FVG) was ordered from CPC Scientific

(3) Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancerNat CommunApril 15, 2026Yapeng Su, Chunmei Liu, Xiang Lu, Hui-Yu Chuang, Guideng Li, Shiqun Shao, Yan Kong, Jihoon W. Lee, Rachel H. Ng, Stephanie Wong, et al.9806S), purified using spin columns from the Serine/Threonine Phosphatase Assay Kit (Promega, Cat# V2460) according to the manufacturer’s protocol, and quantified using the protein BCA assay. The assay determines

(4) The Adiponectin‐ PP2A Pathway Confers Cognitive Benefits of Physical Exercise Against Chronic Stress‐Induced Tau Hyperphosphorylation in the HippocampusAging CellMarch 17, 2026Hui‐Hui Guo, Hai‐Ning Ou, Jia‐Sui Yu, Zi‐Rui Luo, Suk‐Yu Yau, Hector Wing‐Hong TsangAssay To evaluate PP2A activity in the hippocampus, taserine/threonine phosphatase assay system kit V2460 (Promega, Madison, WI, USA) was used following the manufacturer's instructions (Lei et al. 2022). Endogenous

(5) FL7 is an ancient ABA-independent inhibitor of PP2C-As regulating plant stress responsesNat CommunDecember 15, 2025Tianli Li, Zitong Yang, Guojun Li, Jan de Vries, Yuke Li, Xianglan Li, Xu Lu, Xinyi Gu, Zhaojie Chen, Yang Zhao, et al.PP2C-A activity In vitro PP2C-A activity was measured using a Ser/Thr Phosphatase Assay Kit (Promega, V2460). In brief, the recombinant proteins were purified with buffers that did not contain phosphate. To measure

(6) The Arabidopsis phosphatase PP2C12 negatively regulates LRX-RALF-FER-mediated cell wall integrity sensingThe EMBO JournalNovember 17, 2025Xiaoyu Hou, Kyle W Bender, Álvaro D Fernández-Fernández, Gabor Kadler, Shibu Gupta, Mona Häfliger, Amandine Guérin, Anouck Diet, Stefan Roffler, Daniela Campanini, et al.enzymes,NEB, LR recombinase,Invitrogen, "RALF1 peptide, synthetic",PhtdPeptides, Phosphatase assay kit,Promega,V2460 Software,Software,Software ImageJ,, Other,Other,Other PVDF membranes,"Immobilon_P, Millipore",IPVH00010 NC

(7) Poltergeist-Like 2 (PLL2)-dependent activation of herbivore defence distinguishes systemin from other immune signalling pathwaysNat PlantsJuly 4, 2025Rong Li, Xu Wang, Fatima Haj Ahmad, Anja Thoe Fuglsang, Anke Steppuhn, Annick Stintzi, Andreas Schallerphosphatase activity assays were performed using the Serine/Threonine Phosphatase Assay Kit (Promega, V2460), with the bead volume adjusted to result in equal amounts of enzyme for each of the PLL2 variants (Extended

(8) GhTOPP4aD and GhRAF36 inversely regulate cotton (Gossypium hirsutum) response to ABA and salt stress through reversible phosphorylation of GhABI1Plant Biotechnology JournalJune 8, 2025Pengfei Cao, Lin Zhou, Mingwei Du, Xiaoli Tian, Fangjun Li, Zhaohu Limeasured at 405 nm. PP2C phosphatase activity was measured using a Ser/Thr Phosphatase Assay Kit (Promega; V2460) as described (Park et al., 2009). In brief, the corresponding CDS of GhTOPP4aD, GhABI1, GhABI1 T124A

(9) A network-based modeling framework reveals the core signal transduction network underlying high carbon dioxide-induced stomatal closure in guard cellsPLOS BiologyMay 1, 2024Xiao Gan, Palanivelu Sengottaiyan, Kyu Hyong Park, Sarah M. Assmann, Réka AlbertABI2 phosphatase activity was assayed using the Ser/Thr phosphatase assay kit (Promega; catalog number V2460). The reaction was performed in a 50 μl volume containing 28 nM of purified recombinant GST-ABI2 protein

(10) Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer’s disease miceProtein & CellApril 1, 2024He-Zhou Huang, Wen-Qing Ai, Na Wei, Ling-Shuang Zhu, Zhi-Qiang Liu, Chao-Wen Zhou, Man-Fei Deng, Wen-Tao Zhang, Jia-Chen Zhang, Chun-Qing Yang, et al.activity in the supernatants by centrifuge was tested using the Serine/Threonine Phosphatase Assay kit (V2460, Promega, Madison, USA) according to the manufacturer’s protocol. Briefly, the high concentration of

(11) GlPRMT5 inhibits GlPP2C1 via symmetric dimethylation and regulates the biosynthesis of secondary metabolites in Ganoderma lucidumCommunications BiologyFebruary 28, 2024Zi Wang, Hao Qiu, Yefan Li, Mingwen Zhao, Rui Liuazide). The assay was then performed using the Serine/Threonine Phosphatase Assay System (Promega, Cat# V2460) as directed by the manufacturer. After the reaction was complete, the absorbance was measured on a

(12) Maintaining Toll signaling in Drosophila brain is required to sustain autophagy for dopamine neuron survivaliScienceFebruary 16, 2024Zhang J, Tang T, Zhang R, Wen L, Deng X, Xu X, Yang W, Jin F, Cao Y, Lu Y, et al.assay PP2A activity in S2 cells was determined using the serine/threonine phosphatase assay system (Promega, V2460) according to the manufacturer’s instruction. Briefly, S2 cells were collected and lysed in phosphatase

(13) CUL4B mutations impair human cortical neurogenesis through PP2A-dependent inhibition of AKT and ERKCell Death & DiseaseFebruary 8, 2024Yanyan Ma, Xiaolin Liu, Min Zhou, Wenjie Sun, Baichun Jiang, Qiao Liu, Molin Wang, Yongxin Zou, Qiji Liu, Yaoqin Gong, et al.measured. PP2A activity was determined using the Serine/Threonine Phosphatase Assay System (Promega, Cat# V2460) according to the manufacturer’s manual. Quantitative Real Time (qRT)-PCR Total RNA was extracted

(14) PP2Ac knockdown attenuates lipotoxicity‑induced pancreatic β‑cell dysfunction and apoptosisExperimental and Therapeutic MedicineOctober 10, 2023Zhengwei Zhang, Beier Tong, Jie Liu, Jieyuan Feng, Linyang Song, Huawei Wang, Mengting Ke, Chengkai Xu, Yancheng Xuactivity analysis PP2A activity was measured using a Serine/Threonine Phosphatase Assay System (cat. no. V2460; Promega Corporation). Endogenous phosphate was removed by adding cell or tissue lysates to Sephadex®

(15) CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer's disease gene expression linked to neuropathologyScience advancesMarch 3, 2023Gouveia Roque C, Chung KM, McCurdy EP, Jagannathan R, Randolph LK, Herline-Killian K, Baleriola J, Hengst U.initially lysed in accordance with the instructions provided in the Serine/Threonine Phosphatase Assay Kit (V2460, Promega). Lysates were then centrifuged at 1 × 105 g at 4°C for 1 hour in phosphatase storage buffer

(16) Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassaElifeJanuary 10, 2023Anita Szőke, Orsolya Sárkány, Géza Schermann, Orsolya Kapuy, Axel CR Diernfellner, Michael Brunner, Norbert Gyöngyösi, Krisztina Káldianalysis. Phosphatase assay Measuring PP2A activity was carried out with a Ser/Thr Phosphatase Assay System (Promega #V2460) according to the manufacturer’s instructions. Each reaction contained 10 μg protein and was performed

(17) Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassaeLifeJanuary 10, 2023Anita Szőke, Orsolya Sárkány, Géza Schermann, Orsolya Kapuy, Axel C R Diernfellner, Michael Brunner, Norbert Gyöngyösi, Krisztina Káldianalysis. Phosphatase assay Measuring PP2A activity was carried out with a Ser/Thr Phosphatase Assay System (Promega #V2460) according to the manufacturer’s instructions. Each reaction contained 10 μg protein and was performed

(18) HIF-1α Causes LCMT1/PP2A Deficiency and Mediates Tau Hyperphosphorylation and Cognitive Dysfunction during Chronic HypoxiaInternational journal of molecular sciencesDecember 17, 2022Lei L, Feng J, Wu G, Wei Z, Wang JZ, Zhang B, Liu R, Liu F, Wang X, Li HL.primers were shown in Table 2. 4.8. PP2A Activity Assay A Serine/Threonine Phosphatase Assay System kit V2460 was used to detect PP2A activity in the primary neurons and brain homogenates according to the manufacturer’s

(19) Novel small molecular compound 2JY-OBZ4 alleviates AD pathology in cell models via regulating multiple targetsAgingOctober 12, 2022Guo Q, Wu G, Huang F, Wei Z, Wang JZ, Zhang B, Liu R, Yang Y, Wang X, Li HL.was utilized to quantitatively analyze the protein bands. PP2A activity assay The phosphatase kit V2460 was used to measure PP2A activity in the cell extracts according to the manufacturer’s procedure (Promega,

(20) Chk1 Inhibition Ameliorates Alzheimer's Disease Pathogenesis and Cognitive Dysfunction Through CIP2A/PP2A SignalingNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeuticsMarch 1, 2022Hu W, Wang Z, Zhang H, Mahaman YAR, Huang F, Meng D, Zhou Y, Wang S, Jiang N, Xiong J, et al.prepared. PP2A activity in the supernatants was assayed using the Serine/Threonine Phosphatase Assay kit V2460 according to the manufacturer’s procedure (Promega, Madison, USA). Briefly, endogenous free phosphate

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Serine/Threonine Phosphatase Assay System

Serine/Threonine Phosphatase Assay System from Promega

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Serine/Threonine Phosphatase Assay System from Promega

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