Wash Buffer Concentrate, 25X from R&D Systems, a Bio-Techne Brand

Product Specs
ItemWash Buffer Concentrate, 25X
CompanyR&D Systems, a Bio-Techne Brand
Price $270.00Supplier Page View Company Product Page
Catalog NumberWA126
Quantity500 mL
ApplicationsELISA
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
TypeBuffer

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Safety and biodistribution of intrathecal administration of mesenchymal stem cells (MSCs) and neurotrophin-releasing nanoparticles in a porcine CSF-guided delivery model for amyotrophic lateral sclerosis (ALS) drug discoveryScientific ReportsFebruary 27, 2026Emilia Sinderewicz, Maria Dąbkowska, Anna Sarnowska, Joanna Staszkiewicz-Chodor, Dorota Mystkowska, Piotr Holak, Igor Drozd, Magdalena Chodkowska-Michalowska, Monika Rytel, Edyta Paczkowska, et al.commercially available enzyme-linked immunosorbent (ELISA) assay (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). The temperature of the experiments was kept constant at 298 ± 0.1 K. PEG Poly(ethylene

(2) Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial CellsInternational Journal of Molecular SciencesNovember 24, 2025Kenneth P. Mitton, Wendy A. Dailey, Steven Q. Krikor, Kimberly A. Drenser96-well plate overnight. The next day, the plate was washed with buffered surfactant (R&D Systems # WA126). The surfaces were then blocked with BSA (1% in PBS) or Casein (1% in PBS) and a serial dilution of

(3) Increased circulating heat shock protein Hsp70 serum levels as a potential biomarker in bronchial asthma patientsScientific ReportsNovember 18, 2025Ella Churyukina, Elena Oganesyan, Olga Ukhanova, Inga Kotieva, Marina Gulyan, Elena Koreeva, Ekaterina Portnyaga, Danila Bobkov, Stephanie E. Combs, Maxim Shevtsovleft overnight at room temperature. The antibodies were washed three times with wash buffer (cat. no. WA126, R&D Systems, USA). Then 300 µl of blocking buffer (cat. no. DY994, R&D Systems, USA) were added to

(4) Diabetic hyperglycemia-induced glycation regulates tumor vasculature integrity via NF-κB-mediated GM-CSF secretion by tumor cellsCell BiomaterialsOctober 1, 2025Wenjun Wang, Lauren A. Hapach, Paul V. Taufalele, Madison E. Bates, Yusheng Wu, Cynthia A. Reinhart-KingR&D) respectively. Plates were incubated overnight at 4°C, washed three times with washing buffer (WA126, R&D), and blocked with reagent dilute (841380, R&D) for 2 hours at room temperature. After blocking,

(5) Heart Failure Impacts Endothelial Cell Responses to Cardiac Surgery on Cardiopulmonary BypassCellsAugust 31, 2025Olga Papazisi, Rudmer J. Postma, Richard J. Dirven, Saskia L. M. A. Beeres, Remco R. Berendsen, Sesmu M. Arbous, Robert J. M. Klautz, Marieke E. van Vessem, Roel Bijkerk, Jan H. N. Lindeman, et al.commercially available Solid Phase Sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; SANG-20 + WA126) according to the manufacture’s instruction. Plasma vWF levels were determined using an inhouse-developed

(6) Development of a Miniaturized, Automated, and Cost-Effective Device for Enzyme-Linked Immunosorbent AssaySensors (Basel)August 24, 2025Majid Aalizadeh, Shuo Yang, Suchithra Guntur, Vaishnavi Potluri, Girish Kulkarni, Xudong FanMethods 2.1. Materials The Human IL-6 DuoSet ELISA kit (DY206) and the 25X Wash Buffer Concentrate (WA126) are purchased from R&D Systems, Minneapolis, MN, USA. The Blocker BSA (10% in PBS, 37525), the SuperBlock

(7) Inclusion of a retroviral protease enhances the immunogenicity of VLP-forming mRNA vaccines against HIV-1 or SARS-CoV-2 in miceScience Translational MedicineApril 30, 2025Peng Zhang, Mamta Singh, Vada A. Becker, Jacob Croft, Yaroslav Tsybovsky, Vinay Gopan, Yuna Seo, Qingbo Liu, Denise Rogers, Huiyi Miao, et al.and incubated for 1 hour at 37°C. After repeated washing with washing buffer (R&D Systems, Cat. no. WA126), serially diluted mouse sera (5-fold dilutions, 7 steps from a starting dilution of 1:100) were incubated

(8) Proteolytic shedding of CD46 from human hepatocytes indicates liver stressHeliyonDecember 15, 2024Kupke P, Yang Zhou J, Glehr G, Riquelme P, Scheibert L, Adenugba A, Schlitt HJ, Geissler EK, Werner JM, Hutchinson JA.After every step the plate is washed three times by decanting and filling the wells with wash buffer (WA126, R&D systems) using a squirt bottle. First, 100 μl/well of coating antibody (4 μg/ml, MAB2005, R&D systems)

(9) Rapid Microfluidic Immuno-Biosensor Detection System for the Point-of-Care Determination of High-Sensitivity Urinary C-Reactive ProteinBiosensorsMay 30, 2024Chen SJ, Lu SY, Tseng CC, Huang KH, Chen TL, Fu LM.diluent concentrate 2 (DY995), ELISA plate-coating buffer (DY006), and Quantikine ELISA wash buffer 1 (WA126). All the reagents were purchased from R&D Systems (Minneapolis, MN, USA). The reagent kits contained

(10) The effects of urolithin A on poly I:C-induced microglial activationFrontiers in cellular neuroscienceJanuary 1, 2024Mingo YB, Gabele L, Lonnemann N, Brône B, Korte M, Hosseini S.temperature. Plates were washed three times with 0.05% v/v Tween 20 in 1X PBS wash buffer (R&D Systems, Cat. WA126) and blocked in 300 μL 1% BSA in PBS reagent dilution (R&D Systems, Cat. DY995) for 1 h at room temperature.

(11) Chronic Glaucoma Induced in Rats by a Single Injection of Fibronectin-Loaded PLGA Microspheres: IOP-Dependent and IOP-Independent NeurodegenerationInternational journal of molecular sciencesDecember 19, 2023Munuera I, Aragon-Navas A, Villacampa P, Gonzalez-Cela MA, Subías M, Pablo LE, Garcia-Feijoo J, Herrero-Vanrell R, Garcia-Martin E, Bravo-Osuna I, et al.Fibronectin (CF 1918-FN), fibronectin ELISA and ELISA reactants (Reagent Diluent DY995, Wash Buffer WA126, Substrate Reagent Pack DY999, and Stop Solution DY994) were obtained from R&D Systems (Minneapolis,

(12) The Impact of Serum Protein Adsorption on PEGylated NT3-BDNF Nanoparticles-Distribution, Protein Release, and Cytotoxicity in a Human Retinal Pigmented Epithelial Cell ModelPharmaceuticsAugust 30, 2023Dąbkowska M, Kosiorowska A, Machaliński B.commercially available enzyme-linked immunosorbent assay (ELISA) assay (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). The temperature of the experiments was kept at a constant value equal to

(13) Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal CellsCellsAugust 19, 2023Jakl V, Popp T, Haupt J, Port M, Roesler R, Wiese S, Friemert B, Rojewski MT, Schrezenmeier H.

(14) Increased neutralization potency and breadth elicited by a SARS-CoV-2 mRNA vaccine forming virus-like particlesProceedings of the National Academy of Sciences of the United States of AmericaJuly 18, 2023Zhang P, Falcone S, Tsybovsky Y, Singh M, Gopan V, Miao H, Seo Y, Rogers D, Renzi I, Lai YT, et al.incubated for 2 h at room temperature. The plates were washed with ELISA wash buffer (R&D System, Cat # WA126), and the secondary conjugated antibody (goat anti-mouse Invitrogen Cat#A16072) was added and incubated

(15) Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheresDrug DeliveryDecember 31, 2022Alba Aragón-Navas et al.(Barcelona, Spain). Fibronectin (FN), fibronectin ELISA and reactants (Reagent Diluent DY995, Wash Buffer WA126, Stop Solution DY994 and Substrate Reagent Pack DY999) were purchased from R&D Systems (Minneapolis,

(16) Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucomaScientific reportsNovember 30, 2022Rodrigo MJ, Bravo-Osuna I, Subias M, Montolío A, Cegoñino J, Martinez-Rincón T, Mendez-Martinez S, Aragón-Navas A, Garcia-Herranz D, Pablo LE, et al.USA) (purity > 98%). Fibronectin, fibronectin ELISA and reactants (Reagent Diluent DY995, Wash Buffer WA126, Stop Solution DY994 and Substrate Reagent Pack DY999) were purchased from R&D Systems (Minneapolis,

(17) AGE-breaker ALT711 reverses glycation-mediated cancer cell migrationSoft MatterNovember 16, 2022Matthew M. Rowe, Wenjun Wang, Paul V. Taufalele, Cynthia A. Reinhart-KingR&D), respectively. Plates were incubated overnight at 4 °C, washed three times with washing buffer (WA126, R&D), and blocked with reagent dilute (841380, R&D) for 2 hours at room temperature. After blocking,

(18) HBD-2 binds SARS-CoV-2 RBD and blocks viral entry: Strategy to combat COVID-19iScienceMarch 18, 2022Zhang L, Ghosh SK, Basavarajappa SC, Chen Y, Shrestha P, Penfield J, Brewer A, Ramakrishnan P, Buck M, Weinberg A.Microplate,RayBiotech,Cat#CoV-SACE2 Streptavidin-HRP,R&D Systems,Cat#DY998 Quantikine ELISA Wash Buffer 1,R&D Systems,Cat# WA126

(19) Rapid and Quantitative In Vitro Evaluation of SARS-CoV-2 Neutralizing Antibodies and NanobodiesAnalytical chemistryMarch 15, 2022Wu W, Tan X, Zupancic J, Schardt JS, Desai AA, Smith MD, Zhang J, Xie L, Oo MK, Tessier PM, et al.second. 2. Materials 1× PBS buffer (DY006), 10% BSA buffer (SLBR9934V), and wash buffer concentrate (WA126) were purchased from R&D Systems. Streptavidin poly-HRP (PI21140), Poly-HRP dilution buffer (ENN500),

(20) The role of the electrokinetic charge of neurotrophis-based nanocarriers: protein distribution, toxicity, and oxidative stress in in vitro settingJournal of NanobiotechnologyDecember 1, 2021Maria Dąbkowska, Zofia Ulańczyk, Karolina Łuczkowska, Dorota Rogińska, Anna Sobuś, Monika Wasilewska, Maria Olszewska, Katarzyna Jakubowska, Bogusław Machalińskicharge core were conducted by solution depletion ELISA technique (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). Afterward, to determine unbound BDNF molecules more accurately after adsorption

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