Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA DYC1663-2 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $495.00Supplier Page View Company Product Page
Catalog NumberDYC1663-2
Quantity1 Kit (for 2 Plates) (also 1 Kit (for 5 Plates), 1 Pack (for 15 Plates))
ApplicationsELISA
Detection Range156.00 - 10000 pg/mL
ReactivityHuman, Mouse, Rat
Detection TargetHSP70/HSPA1A
Assay Time4 hours 40 mins (after plate preparation)
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameheat shock protein family A (Hsp70) member 1A
NCBI Gene AliasesHEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Increased circulating heat shock protein Hsp70 serum levels as a potential biomarker in bronchial asthma patientsScientific ReportsNovember 18, 2025Ella Churyukina, Elena Oganesyan, Olga Ukhanova, Inga Kotieva, Marina Gulyan, Elena Koreeva, Ekaterina Portnyaga, Danila Bobkov, Stephanie E. Combs, Maxim Shevtsovassay for Hsp70 serum detection The Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA kit (cat. no. DYC1663-2, R&D Systems, USA) was used to set up the sandwich ELISA. A 96-well microtiter plate was pre-coated

(2) Heat Stress Nephropathy in CKD of Uncertain Etiology Hotspots of Bargarh District Odisha, IndiaKidney international reportsOctober 1, 2025Biswas P, Sahu AK, Shristi S, Behera TK, Sahoo SK, Ahmed SN, Mohanty SK, Pati SS, Murugesan K, Mallick N, et al.

(3) Biomarkers in Adult-Type Diffuse Gliomas: Elevated Levels of Circulating Vesicular Heat Shock Protein 70 Serve as a Biomarker in Grade 4 Glioblastoma and Increase NK Cell Frequencies in Grade 3 GliomaBiomedicinesDecember 7, 2023Lennartz P, Thölke D, Bashiri Dezfouli A, Pilz M, Lobinger D, Messner V, Zanth H, Ainslie K, Kafshgari MH, Rammes G, et al.

(4) Protective effects of Althaea officinalis L. extract against N-diethylnitrosamine-induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti-inflammatory, mitochondrial apoptosis and PI3K/Akt/mTOR signaling pathwaysFood science & nutritionAugust 1, 2023Wang Z, Jiang X, Zhang L, Chen H.number: NBP2‐68196), albumin (ALB; catalog number: NBP2‐60483), total protein (TP; catalog number: DYC1663‐2), C‐reactive protein (CRP; catalog number: DY1744), and bilirubin (BIL; catalog number: NBP2‐69939)

(5) First‐trimester plasma extracellular heat shock proteins levels and risk of preeclampsiaJournal of Cellular and Molecular MedicineMay 1, 2023Claudia Melina Robellada‐Zárate, Janelly Estefania Luna‐Palacios, Carlos Agustín Zapata Caballero, Juan Pablo Acuña‐González, Irlando Lara‐Pereyra, Diego Iván González‐Azpeitia, Ricardo Josué Acuña‐González, Elsa Romelia Moreno‐Verduzco, Héctor Flores‐Herrera, Mauricio Osorio‐CaballeroeHsp‐27 (DYC‐1580; R&D Systems, Minneapolis, MN, USA), eHsp‐60 (DYC1800‐2; R&D Systems) and eHsp‐70 (DYC1663‐2; R&D System) according to the commercial manufacturer's specifications as previously reported by our

(6) The Stability and Anti-Angiogenic Properties of Titanium Dioxide Nanoparticles (TiO(2)NPs) Using Caco-2 CellsBiomoleculesSeptember 21, 2022David OM, Lategan KL, de Cortalezzi MF, Pool EJ.

(7) Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal SepsisFrontiers in PediatricsNovember 25, 2021Arturo Alejandro Canul-Euan, Gibran Zúñiga-González, Janelly Estefania Palacios-Luna, Rolando Maida-Claros, Néstor Fabián Díaz, Patricia Saltigeral-Tigeral, Perla Karina García-May, Oscar Díaz-Ruiz, Héctor Flores-Herreraof eHsp-27 (DYC-1580, R&D Systems, Minneapolis, MN, USA), eHsp-60 (DYC1800-2, R&D Systems), eHsp-70 (DYC1663-2, R&D System), and TNFα (DY210, R&D System). The plasma used for the quantified was not diluted. Standard

(8) Belantamab Mafodotin (GSK2857916) Drives Immunogenic Cell Death and Immune-mediated Antitumor Responses In VivoMolecular cancer therapeuticsOctober 1, 2021Montes de Oca R, Alavi AS, Vitali N, Bhattacharya S, Blackwell C, Patel K, Seestaller-Wehr L, Kaczynski H, Shi H, Dobrzynski E, et al.markers were quantified from cell supernatants by the following ELISAs: HSP70 (R&D Systems; catalog No. DYC1663-2; 1:2 sample dilution), HSP90α (Invitrogen; catalog No. BMS2090; 1:25 sample dilution), IL8 (R&D Systems;

(9) Intralesional injection of rose bengal augments the efficacy of gemcitabine chemotherapy against pancreatic tumorsBMC cancerJune 30, 2021Innamarato P, Morse J, Mackay A, Asby S, Beatty M, Blauvelt J, Kidd S, Mullinax JE, Sarnaik AA, Pilon-Thomas S.

(10) Effects of Bovine Colostrum with or without Egg on In Vitro Bacterial-Induced Intestinal Damage with Relevance for SIBO and Infectious DiarrheaNutrientsMarch 22, 2021Playford RJ, Choudhry N, Kelly P, Marchbank T.

(11) Mitoxantrone-Loaded Nanoparticles for Magnetically Controlled Tumor Therapy-Induction of Tumor Cell Death, Release of Danger Signals and Activation of Immune CellsPharmaceuticsSeptember 27, 2020Ratschker T, Egenberger L, Alev M, Zschiesche L, Band J, Schreiber E, Frey B, Derer A, Alexiou C, Janko C.

(12) Vitamins Modulate the Expression of Antioxidant Genes in Progesterone-Treated Pancreatic β Cells: Perspectives for Gestational Diabetes ManagementInternational Journal of EndocrinologySeptember 15, 2020Nathália Ruder Borçari, Jeniffer Farias dos Dos Santos, Gustavo Roncoli Reigado, Bruna Letícia Freitas, Mariana da Silva Araújo, Viviane Abreu Nuneswas determined using Human Total HSP70/HSPA1A DuoSet IC ELISA (enzyme-linked immunosorbent assay) kit DYC1663-2 (R&D Systems Inc., Minneapolis, USA) according to the manufacturer's instructions and following the

(13) A tumor-intrinsic PD-L1/NLRP3 inflammasome signaling pathway drives resistance to anti-PD-1 immunotherapyThe Journal of clinical investigationMay 1, 2020Theivanthiran B, Evans KS, DeVito NC, Plebanek M, Sturdivant M, Wachsmuth LP, Salama AK, Kang Y, Hsu D, Balko JM, et al.https://prod.tbilab.org/balko_lab/LAG3_JCI_Insight (53). ELISA. Levels of CXCL5 and HSP70 (R&D Systems, DYC16632) in mouse plasma were evaluated using an ELISA kit according to the manufacturer’s instructions (R&D

(14) Differences of the Immune Phenotype of Breast Cancer Cells after Ex Vivo Hyperthermia by Warm-Water or Microwave Radiation in a Closed-Loop System Alone or in Combination with RadiotherapyCancersApril 27, 2020Hader M, Savcigil DP, Rosin A, Ponfick P, Gekle S, Wadepohl M, Bekeschus S, Fietkau R, Frey B, Schlücker E, et al.

(15) Radiosensitization of HSF-1 Knockdown Lung Cancer Cells by Low Concentrations of Hsp90 Inhibitor NVP-AUY922CellsSeptember 28, 2019Kühnel A, Schilling D, Combs SE, Haller B, Schwab M, Multhoff G.

(16) Systemic release of heat-shock protein 27 and 70 following severe traumaScientific reportsJuly 3, 2019Haider T, Simader E, Glück O, Ankersmit HJ, Heinz T, Hajdu S, Negrin LL.

(17) Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathwayEBioMedicineJuly 1, 2019Nunes NS, Chandran P, Sundby M, Visioli F, da Costa Gonçalves F, Burks SR, Paz AH, Frank JA.

(18) Chemo-photothermal therapy combination elicits anti-tumor immunity against advanced metastatic cancerNature communicationsMarch 14, 2018Nam J, Son S, Ochyl LJ, Kuai R, Schwendeman A, Moon JJ.

(19) Oncolytic Group B Adenovirus Enadenotucirev Mediates Non-apoptotic Cell Death with Membrane Disruption and Release of Inflammatory MediatorsMolecular therapy oncolyticsMarch 17, 2017Dyer A, Di Y, Calderon H, Illingworth S, Kueberuwa G, Tedcastle A, Jakeman P, Chia SL, Brown A, Silva MA, et al.

(20) Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammationProceedings of the National Academy of Sciences of the United States of AmericaJanuary 3, 2017Kovacs ZI, Kim S, Jikaria N, Qureshi F, Milo B, Lewis BK, Bresler M, Burks SR, Frank JA.(SDF1) (RS0074; NeoBiolab), BDNF (ELR-BDNF; RayBiotech, Inc.), MMP9; FGF (MFB00), ICAM (RIC100), HSP70 (DYC1663-2), and hepatocyte growth factor (HGF) (MHG00), all from R&D Systems, Inc. All assays were performed Kovacs

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Total HSP70/HSPA1A ELISA