Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer E2000 from Promega

Product Specs
ItemBeta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
CompanyPromega
Price $101.00Supplier Page View Company Product Page
Catalog NumberE2000
Detection Targetbeta-galactosidase
Assay TimeAt least 30 minutes
Sample TypeCells lysates
Quantity10ml
MethodColorimetric
Molecular Weight~70kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Preclinical evaluation of cysteine protease-inhibitor aloxistatin (E64d) for heart failure therapyJ Mol Med (Berl)June 25, 2026Maria Jordan, Angelika Stucki-Koch, Maximilian Schinke, Lena Willmer, Ingrid Senne, Naisam Abbas, Marco Bentele, Dana Oldenkott, Nico Lachmann, Linda Feldbrügge, et al.Cell Culture Lysis Reagent (E1500, Luciferase Assay System, Promega) and 50 µL of Assay 2 × Buffer (E2000, β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer, Promega) was added. After 30 min incubation

(2) Epithelial sensing of vitamin A shapes intestinal antimicrobial defensebioRxivMarch 13, 2026Gabriella Quinn, Daniel C. Propheter, Kartik Kulkarni, Matthew Johnson, Gonçalo Vale, Jeffrey G. McDonald, Ann Johnson, Brian Hassell, Cassie L. Behrendt, Nikhil V. Munshi, et al.C01010055 BrightGlo Luciferase Assay System,Promega,Ref. E2610 β-Galactosidase Enzyme Assay System,Promega,Ref. E2000 Deposited data,Deposited data,Deposited data scRNAseq Mouse Small Intestine,This study,GSE318054 HCT-116

(3) CI-994 is a dual modulator of class I HDACs and Wnt/β-catenin signaling for the treatment of Alzheimer's diseaseAlzheimer's Research & TherapyFebruary 7, 2026Wenyan Lu, Keiji Kawatani, Yingxue Ren, Toshihiko Nambara, Lin Jia, Suren Jeevaratnam, Eunmi Lee, Paula Rodriguez Martinez, Taha Izhar, Ni Wang, et al.E1081). The luciferase assay system (cat. no. E1500) and β-galactosidase enzyme assay system (cat. no. E2000) were purchased from Promega. HEK293 cells were plated into 24-well plates. After overnight culture,

(4) TMEM33 deletion potentiates anti-tumor CD8 + T cell immunitybioRxivJanuary 5, 2026Matthew T Jackson, Tianming Zhao, Gusztav Milotay, Isabela Pedroza-Pacheco, Yanshu Cai, Claire Willis, Su M Phyu, Bruno Beernaert, Jamie TW Kwon, Hala Estephan, et al.PBS. B3Z lysates were prepared and processed using the Promega β-Galactosidase Enzyme Assay System (#E2000), according to the manufacturer’s instructions. Absorbance was measured at 420 nm using a POLARstar OMEGA

(5) Circular RNA circAtxn10 regulates skeletal muscle cell differentiation by targeting miR-143-3p and Chrna1The Korean Journal of Physiology & PharmacologySeptember 1, 2025Nakwon Choe, Anna Jeong, Hosouk Joung, Dongtak Jeong, Young-Kook Kim, Hyun Kook, Duk-Hwa Kwonwas also co-transfected to normalize the luciferase signal with β-Galactosidase Enzyme Assay System (E2000, Promega). Statistical analysis Analyses were conducted using IBM SPSS Statistics 26 (IBM). Data normality

(6) ATG conjugation–dependent/independent mechanisms underlie lysosomal stress–induced TFEB regulationJ Cell BiolAugust 29, 2025Shiori Akayama, Takayuki Shima, Tatsuya Kaminishi, Mengying Cui, Jlenia Monfregola, Kohei Nishino, Andrea Ballabio, Hidetaka Kosako, Tamotsu Yoshimori, Shuhei Nakamuracommercially available luciferase assay system (E4030; Promega) and β-galactosidase enzyme assay system (E2000; Promega). Statistical analysis and reproducibility All quantifications are presented as the means

(7) Imiquimod, a Promising Broad-Spectrum Antiviral, Prevents SARS-CoV-2 and Canine Coronavirus Multiplication Through the MAPK/ERK Signaling PathwayVirusesMay 31, 2025Josefina Vicente, Freddy Armando Peñaranda Figueredo, Stefania Mantovani, Daniela Laura Papademetrio, Sergio Ivan Nemirovsky, Andrea Alejandra Barquero, Carina Shayo, Carlos Alberto Buenoquantitation was performed using the Luciferase Assay System E1500 and β-Galactosidase Enzyme Assay System E2000 (Promega, Madison, WI, USA), following the manufacturer’s instructions. Measurements were obtained using

(8) ARHGEF3 coordinates adipocyte hypertrophy and differentiation through dual YAP-RhoA and PPARγ activationJournal of Advanced ResearchApril 9, 2025Sana Abdul Khaliq, Shi-Young Park, Syeda Maham, Yoonil Cho, Miseon Lee, Seungyoon Nam, Je Kyung Seong, Jie Chen, Cheol Soo Choi, Mee-Sup YoonConcurrently, β-galactosidase activity was measured using the β-galactosidase Enzyme Assay System (E2000, Promega). Luminescence intensity was recorded with a Victor NivoTM multimode microplate reader (PerkinElmer).

(9) Blockade of ZMIZ1‐GATA4 Axis Regulation Restores Youthfulness to Aged CartilageAdvanced ScienceMarch 5, 2025Jiho Nam, Hyunmin Woo, Jihye Yang, Seok Jung Kim, Kwang Pyo Lee, Ji Hoon Yu, Tae Joo Park, Seong‐il Eyun, Siyoung Yangcell extracts using a luciferase assay kit (Promega, E1501) and a β‐galactosidase assay kit (Promega, E2000) according to the manufacturer's recommendations. Luciferase activity was normalized to β‐galactosidase

(10) SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activationScientific ReportsJuly 16, 2024Kevin Schmidt, Arne Schmidt, Sonja Groß, Annette Just, Angelika Pfanne, Maximilian Fuchs, Maria Jordan, Elisa Mohr, Andreas Pich, Jan Fiedler, et al.normalization, β-galactosidase activity was determined using the β-Galactosidase Enzyme Assay System (E2000, Promega) according to manufacturer’s protocol. Absorbance was detected at a Synergy HT. Treatment

(11) Aesculus hippocastanum extract and the main bioactive constituent β-escin as antivirals agents against coronaviruses, including SARS-CoV-2Sci RepMarch 17, 2024Freddy Armando Peñaranda Figueredo, Josefina Vicente, Andrea Alejandra Barquero, Carlos Alberto Buenoviral RSV promoter. Luciferase Assay System E1500 (Promega) and β-Galactosidase Enzyme Assay System E2000 (Promega) were used for reporter quantitation following the manufacturer’s instructions. Statistical

(12) DNA ultra-sensitive quantification, a technology for studying HIV unintegrated linear DNACell Reports MethodsApril 24, 2023Hélène Marie Roux, Suzanne Figueiredo, Lucas Sareoua, Maud Salmona, Juliette Hamroune, Lucie Adoux, Julie Migraine, Allan Hance, François Clavel, Rémi Cheynier, et al.assays ,, Lenti-X p24 Rapid Titer kit,Takara Bio,Cat# 632200 β-galactosidase enzyme assay system,Promega,Cat# E2000 EasySep Human Resting CD4+ T-cell Isolation Kit,Stemcell,Cat# 17962 Kapa Library quantification Kit,Roche,Cat#

(13) The Flavonoid Cyanidin Shows Immunomodulatory and Broad-Spectrum Antiviral Properties, Including SARS-CoV-2VirusesApril 18, 2023Josefina Vicente, Martina Benedetti, Paula Martelliti, Luciana Vázquez, María Virginia Gentilini, Freddy Armando Peñaranda Figueredo, Mercedes Soledad Nabaes Jodar, Mariana Viegas, Andrea Alejandra Barquero, Carlos Alberto BuenoArgentina). Luciferase Assay System E1500 (Promega, Madison, WI, USA) and β-Galactosidase Enzyme Assay System E2000 (Promega, Madison, WI, USA) were used for reporter quantitation following the manufacturer’s instructions. 2.12.

(14) Meganuclease targeting HSV-1 protects against herpetic keratitis: Application to corneal transplantsMolecular therapy. Nucleic acidsDecember 13, 2022Chapellier B, Guindolet D, Pereira D, Galetto R, Sahel JA, Labetoulle M, Gabison EE.medium for 24 h before processing for analysis. (1) A β-Galactosidase Enzyme Assay System (Promega kit #E2000; hydrolysis of its colorless substrate ortho-nitrophenyl-β-galactoside, to o-nitrophenol, a yellow chromophore)

(15) Identification of a Novel Anti-HIV-1 Protein from Momordica balsamina Leaf ExtractInternational Journal of Environmental Research and Public HealthNovember 18, 2022Morgan I. Coleman, Mahfuz Khan, Erick Gbodossou, Amad Diop, Kenya DeBarros, Hao Duong, Vincent C. Bond, Virginia Floyd, Kofi Kondwani, Valerie Montgomery Rice, et al.agent in the leaf extract does not inhibit β-gal activity, we used a β-gal assay kit (Promega Cat# E2000) to measure β-gal activity in the presence of extract. We completed the protocol as suggested by the

(16) CK2-mediated phosphorylation of SUZ12 promotes PRC2 function by stabilizing enzyme active siteNature communicationsNovember 9, 2022Gong L, Liu X, Jiao L, Yang X, Lemoff A, Liu X.normalized by β- galactosidase activity using the β-galactosidase Enzyme Assay System (Promega, Cat No. E2000). Western blot using the anti-HA antibody was performed to compare the GAL4-HA-SUZ12 expression level.

(17) Alteration of Trop-2 expression in breast cancer cells by clinically used therapeutic agents and acquired tamoxifen resistanceBreast cancer (Tokyo, Japan)November 1, 2022Zhu J, Wu W, Togashi Y, Taira Nihira N, Johmura Y, Zhu D, Nakanishi M, Miyoshi Y, Ohta T.and normalized to the β-galactosidase activity determined by the β-galactosidase enzyme assay system (E2000; Promega). Cell viability assay Appropriate number of cells (1 × 105 for MCF7 and 8 × 104 for T47D

(18) Microbiome and ileum transcriptome revealed the boosting effects of selenium yeast on egg production in aged laying hensAnimal NutritionSeptember 1, 2022Zhexi Liu, Yutao Cao, Yue Ai, Xiaonan Yin, Linli Wang, Mengyao Wang, Bingkun Zhang, Zhengxing Lian, Keliang Wu, Yuming Guo, et al.performed on ileum of aged laying hens by β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer (E2000, Promega, WI, USA). Lysates were prepared using 1 × Reporter Lysis Buffer and then Assay 2 × Buffer

(19) SRF depletion in early life contributes to social interaction deficits in the adulthoodCellular and molecular life sciences : CMLSMay 4, 2022Roszkowska M, Krysiak A, Majchrowicz L, Nader K, Beroun A, Michaluk P, Pekala M, Jaworski J, Kondrakiewicz L, Puścian A, et al.assays Luciferase and β-Gal activity were evaluated in neuronal cell lysates using commercial assay kits (Promega, #E2000, #1500). Luminescence recordings were performed using an Infinite M200 microplate reader with an injector

(20) A novel peptide inhibitor of Dll4-Notch1 signalling and its pro-angiogenic functionsBritish journal of pharmacologyApril 1, 2022Zhu G, Lin Y, Ge T, Singh S, Liu H, Fan L, Wang S, Rhen J, Jiang D, Lyu Y, et al.activity. β-Gal activity was measured by β-gal enzyme assay system with reporter lysis buffer (Promega, E2000). 2.15 | Western blotting, immunoprecipitation and RT-qPCR HUVECs were lysed in cell lysis buffer

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Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer from Promega

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Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer from Promega

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