TMB Substrate Pack, 250 mL DY999 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTMB Substrate Pack, 250 mL
CompanyR&D Systems, a Bio-Techne Brand
Price $330.00Supplier Page View Company Product Page
Catalog NumberDY999
Quantity1 Pack
ApplicationsELISA
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

Specifications/Features

Available conjugates: Sizes Available: 1 Pack

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Efficacy of intranasal adenovirus vector vaccine is attenuated by type I IFN‒induced NK cell activationMolecular Therapy AdvancesJune 11, 2026Hayato Nakatani, Masashi Tachibana, Rika Onishi, Takato Nakagaki, Ken J. Ishii, Kahori Shimizu, Fuminori Sakurai, Hiroyuki Mizuguchiincubation, the color reaction was developed using a Substrate Reagent Pack (R&D System, Minneapolis, MN, #DY999) and stopped with 0.5 N HCl, and the absorbance was measured at OD450 on a plate reader (TriStar LB

(2) Ultrasensitive non-enzymatic protein detection using proximity immunoassay with photonic resonator absorption microscopynpj BiosensingMay 21, 2026Skye Shepherd, Seemesh Bhaskar, Hui Xu, Huimin Zhang, Weinan Liu, Brian T. Cunninghammixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (R&D Systems, Catalog # DY999) was added for 20 min, then reaction was halted by adding a stop solution of 50 μL of a 2 N H2SO4 solution

(3) Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxinInfection and ImmunityApril 15, 2026Désirée Schaaf, Muriel Dresen, Yenehiwot Berhanu Weldearegay, Jeannine Biermann, Susan L. Brockmeier, Wolfgang Baumgärtner, Michael Jarek, Peter Valentin-Weigandinstructions. Samples were diluted 1:2, 1:10, or 1:20. The Substrate Reagent Pack (R&D Systems, Cat. No. DY999) was used to identify streptavidin-horseradish peroxidase coupled to detection antibodies, and the reaction

(4) IL1β+ macrophages promote intestinal fibrosis in Crohn’s disease by secreting AREG to activate PI16+ fibroblastsJournal of Translational MedicineMarch 14, 2026Hanlin Xie, Xiaofeng Wen, Tao Ma, Lin Wang, Yanxu Guo, Guanzhan Liang, Jing Chen, Ruibing Li, Yuxi Pan, Zhenyu Xian, et al.ELISACalc software. The ELISA kits used in this study were as follows: DY262, DY008B, DY008C, DY999B, DY999, DY999B-250 (R&D Systems). Primary human intestinal fibroblasts isolation Three to four strips of

(5) Safety and biodistribution of intrathecal administration of mesenchymal stem cells (MSCs) and neurotrophin-releasing nanoparticles in a porcine CSF-guided delivery model for amyotrophic lateral sclerosis (ALS) drug discoveryScientific ReportsFebruary 27, 2026Emilia Sinderewicz, Maria Dąbkowska, Anna Sarnowska, Joanna Staszkiewicz-Chodor, Dorota Mystkowska, Piotr Holak, Igor Drozd, Magdalena Chodkowska-Michalowska, Monika Rytel, Edyta Paczkowska, et al.determined using a commercially available enzyme-linked immunosorbent (ELISA) assay (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). The temperature of the experiments was kept constant at 298

(6) Kinetics of local C3 production orchestrates neutrophil recruitment in lung injurybioRxivFebruary 21, 2026Rafael Aponte Aponte Alburquerque, Josue I. Hernandez, Aasritha Nallapu, Marick Starick, Neelou Etesami, Sandip K. Mukherjee, Ayse Naz Ozanturk, Jose Vazquez, Allison Chang, Ashley Zheng, et al.applied and incubated for 1 hour. The plates were then washed three times, and TMB Color Substrate (#DY999, R&D Systems) was added (100 μl per well) and incubated at room temperature (RT) for 10 min. To stop

(7) Pathogenic Role of FGFR3 Autoantibodies in Small Fiber NeuropathyAdvanced ScienceFebruary 17, 2026Lyuba Y. Salih, Nicolas L.A. Dumaire, Clémence Gieré, Erin Vest, Haya Alkhateeb, Christian P. Moritz, Yannick Tholance, Jean‐Philippe Camdessanché, Jean‐Christophe Antoine, Jérôme Honnorat, et al.for 45 min at room temperature. After 3 washes with PBS, 0.1% tween‐20, tetramethylbenzidine (Cat# DY999, R&D Systems) was added as the colorimetric substrate. The reaction was terminated after 45 min with

(8) HIV-induced sialoglycans on infected CD4+ T cells promote immune evasion from myeloid cell-mediated killingNat CommunDecember 13, 2025Shalini Singh, S. M. Shamsul Islam, Rui Liu, Opeyemi S. Adeniji, Lacy M. Simons, Pratima Saini, Hiroaki Tateno, Ali Danesh, Paul W. Denton, Leila B. Giron, et al.(Jackson ImmunoReseach) for 1 h and by addition of HRP chromogenic substrate (R&D Systems, Catalog #DY999), and absorbance was measured at 450 nM. Immune cell mediated killing of HIV-infected CEM.NKR CCR5+

(9) Maternal undernutrition exacerbates microbiota-driven growth stunting through pre- and postnatal effectsCell ReportsNovember 25, 2025Yadeliz A. Serrano Serrano Matos, Claire Williams, Jasmine Cano, Lindsey Bihuniak, Aria Kamal, Carrie A. CowardinJ20605-AP PBS,Fisher BioReagents,Cat# BP2944100 Tetramethylbenzidine (TMB) Solution,R&D Systems,Cat# DY999 Sulfuric Acid,MilliporeSigma,Cat# 339741 Ethanol,MilliporeSigma,Cat# 459844 Glycerol,MilliporeSigma,Cat#

(10) Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial CellsInternational Journal of Molecular SciencesNovember 24, 2025Kenneth P. Mitton, Wendy A. Dailey, Steven Q. Krikor, Kimberly A. Drenserincubated with Streptavidin–HRP (R&D Systems; DY998), followed by more washing. TMB substrate (R&D Systems, DY999) was added to the wells, the plate was stored in the dark, and color development was stopped by the

(11) Increased circulating heat shock protein Hsp70 serum levels as a potential biomarker in bronchial asthma patientsScientific ReportsNovember 18, 2025Ella Churyukina, Elena Oganesyan, Olga Ukhanova, Inga Kotieva, Marina Gulyan, Elena Koreeva, Ekaterina Portnyaga, Danila Bobkov, Stephanie E. Combs, Maxim ShevtsovUSA) was added to each well for 20 min, followed by washing. Next, the substrate solution (cat. no. DY999, R&D Systems, USA) was added, incubated for 20 min, after which the stop solution (cat. no. DY994, R&D

(12) Multiplex Magnetic Biosensor Microarrays Facilitate Personalized Vaccination Schedules for SARS-CoV-2 VariantsBioChip JournalOctober 13, 2025Hyun-Kyung Um, Songeun Kim, Jueun Hwang, Yeeun Lee, Shan X. Wang, Jung-Rok LeeInstruments, USA). For ELISA, horseradish peroxide-conjugated streptavidin (SA-HRP; DY998), color substrates (DY999), and stop solution (DY994) were purchased from R&D Systems, and the absorbance signals were measured

(13) Anti-CV2/CRMP5 autoantibodies as drivers of sensory neuron excitability and pain in ratsNature communicationsAugust 7, 2025Martin L, Stratton HJ, Salih LY, Dumaire N, Gomez K, Do LD, Loya-Lopez S, Tang C, Calderon-Rivera A, Ran D, et al.

(14) Exploring virus-host interactions through combined proteomic approaches identifies BANF1 as a new essential factor for African Swine Fever VirusMolecular & Cellular ProteomicsJuly 22, 2025Juliette Dupré, Katarzyna Magdalena Dolata, Gang Pei, Aidin Molouki, Lynnette C. Goatley, Richard Küchler, Timothy K. Soh, Jens B. Bosse, Aurore Fablet, Mireille Le Dimna, et al.(R&D Systems, DY998), and finally developed with 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (R&D Systems, DY999). After stopping the reaction with 2 N H2SO4, the absorbances were read at 450 nm. The concentration

(15) Activity-attenuated serum albumin-fused interleukin-33 suppresses experimental autoimmune encephalomyelitisCell Rep MedJuly 15, 2025Erica Budina, Joseph W. Reda, Shijie Cao, Elyse A. Watkins, Ani Solanki, Mindy Nguyen, Phillip S. Ang, J. Emiliano Gómez Medellín, Hye-Rin Chun, Colleen R. Foley, et al.LC6060 Clarity Max Western ECL Substrate,Bio-Rad,Cat. #: 1705062 TMB Substrate Reagent,R&D Systems,Cat. #: DY999 T-PER Tissue Protein Extraction Reagent,Thermo Fisher Scientific,Cat. #: 78510 Complete Protease Inhibitor

(16) Investigating the role of soil-transmitted helminth infections in the development of leprosy in endemic regionsFrontiers in ImmunologyJune 25, 2025Vikram Singh, Ravindra P. Turankar, Vinay Kumar Pathak, Itu Singh, Rahul Sharma, Anjana Goel, Utpal SenguptaSolution (1:1 mixture of Color reagent A (H2O2) and Color reagent B (Tetramethylbenzidine) (Cat no. DY999, R&D Systems, USA) was added to each well. The plates were incubated at RT for 20 minutes in the dark

(17) Persistent Activation of Sphingosine-1-Phosphate Receptor 1 by Phytosphingosine-3,4-Cyclic Phosphate Ameliorates Sepsis by Inhibiting Hyperinflammation and Vascular HyperpermeabilityMedComm (2020)June 3, 2025Suhong Duan, Seung-Gook Kim, Jiaying Bao, Hyung-Jin Lim, Joon Woo Kim, Sung-Il Yoon, Young Jun Park, Sanuk Yun, Kye-Seong Kim, Hwa-Ryung Song, et al.measured by adding 100 µL of stabilized hydrogen peroxide and 3,3′,5,5′‐tetramethylbenzidine solution (#DY999; R&D Systems). Using a Molecular Devices microplate reader (Sunnyvale, CA, USA), the absorbance at 450

(18) Precision targeting of β-catenin induces tumor reprogramming and immunity in hepatocellular cancersNature communicationsMay 30, 2025Lehrich BM, Delgado ER, Yasaka TM, Liu S, Cao C, Liu Y, Taheri MN, Guan X, Koeppen H, Singh S, et al.agitation. Working concentration of streptavidin-horse radish peroxidase (HRP) and color substrate mix (DY999, R&D Systems, Minneapolis, MN) were incubated at room temperature for 20 min with slight agitation.

(19) Brain derived neurotrophic factor and secreted amyloid precursor protein-α response to moderate and high intensity exercisePhysiological reportsMay 1, 2025Spencer FSE, Elsworthy RJ, Breen L, Bishop JRB, Lucas SJE, Aldred S.all‐out‐HIIT) were determined by enzyme‐linked immunosorbent assay (ELISA) (Duoset ELISA, Bio‐techne DY999) as per manufacturer's instructions. Levels of sAPPα in serum were also determined by ELISA (IBL America,

(20) Inclusion of a retroviral protease enhances the immunogenicity of VLP-forming mRNA vaccines against HIV-1 or SARS-CoV-2 in miceScience Translational MedicineApril 30, 2025Peng Zhang, Mamta Singh, Vada A. Becker, Jacob Croft, Yaroslav Tsybovsky, Vinay Gopan, Yuna Seo, Qingbo Liu, Denise Rogers, Huiyi Miao, et al.were then washed, and the reaction was developed by adding substrate reagents (R&D Systems, Cat. no. DY999). The reaction was stopped after 15 min by addition of sulfuric acid. Light absorbance at 450 nm was

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Substrate Reagent Pack

Substrate Reagent Pack

TMB Substrate Pack, 250 mL from R&D Systems, a Bio-Techne Brand

Description

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (293)

Reviews

TMB Substrate Pack, 250 mL from R&D Systems, a Bio-Techne Brand

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