Fig 1: Cathelicidin induces IL-17F-producing but not IL-22-producing T cells.Splenocytes isolated from C57BL/6 J mice were cultured in Th17 conditions for 48 h in the presence or absence of 2.5 μM cathelicidin. A–D Expression of IL-17A and F was determined by intracellular flow cytometry. E IL-17F and F IL-22 production was quantified by ELISA of cell culture supernatant after 48 h in culture. Data shown are individual mice in separate experiments. B–F are analysed by paired two-tailed t-tests. SP = single positive. N values: B - 9, C - 9, D - 9, E - 5, F - 4. Black symbols represent untreated samples and open symbols are samples treated with cathelicidin.
Fig 2: Human CD4 + T cells enhance IL-17 production following cathelicidin exposure.CD4 + T cells were isolated from peripheral blood from healthy human donors and cultured for 8 days in Th17-driving conditions, in the presence or absence of 2.5 μM human cathelicidin (LL-37). IL-17A production was assessed by A, B flow cytometry on day 8 and C ELISA on days 3 and 8 of culture. D IL-17F was assessed by flow cytometry and E IL-22 by ELISA. Data shown are individual donors with line at mean. B–E were analysed by a two-sided Wilcoxon test, on raw data before conversion to %. N values: B - 11, C - 11, D - 6, E - 3. Black symbols represent untreated samples, open symbols represent samples exposed to cathelicidin.
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