Comet SCGE assay kit ADI-900-166 from Enzo Life Sciences, Inc.

Product Specs
ItemComet SCGE assay kit
CompanyEnzo Life Sciences, Inc.
Price Supplier Page View Company Product Page
Catalog NumberADI-900-166
Quantity50 tests
MethodAssay
ReactivitySpecies independent
ApplicationsFluorescence microscopy
Assay Time3 hours

Specifications/Features

Fast and simple electrophoresis method to measure DNA fragmentation

Ready-to-use Comet Slides allow direct application of sample without pretreatment
Shorter assay time allows for higher throughput sample analysis
Hydrophobic barrier allows sample treatment with DNA repair enzymes
Unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide

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Enzo Life Sciences, Inc.

10 Executive Blvd.

Farmingdale, NY 11735

United States

Phone: 800-942-0430
Fax: 610-941-9252

Company Profile

Website: http://www.enzolifesciences.com

(1) Dual roles of DEAD-box RNA helicase Brr2 in genome stability regulationNat CommunOctober 15, 2025Xiaolan Chen, Jin You, Qin Ma, Jiamei Lin, Yu Ji, Shi Li, Zhenxing Song, Rui Su, Ge Shan, Chuan Huangthe Source Data file. Comet assay The comet assay was performed using Comet SCGE assay kit (Enzo, ADI-900-166) as previously described56. Cells were washed with ice-cold Ca2+/Mg2+ -free PBS buffer and mixed with

(2) The PCNA inhibitor AOH1996 suppresses cancer stemness and enhances anti-PD1 immunotherapy in squamous cell carcinomaStem Cell Research & TherapySeptember 29, 2025Yujia Wang, Zhen Qin, Yiwen Chen, Baoxin Gu, Lingfei JiaInformation under accession number GSE289770. Comet assay A Comet SCGE Assay Kit (Enzo, NY, USA; Cat# ADI-900-166) was used for single-cell gel electrophoresis [35]. After HNSCC cells were treated with AOH1996 for

(3) Ivosidenib Confers BRCAness Phenotype and Synthetic Lethality to Poly (ADP-Ribose) Polymerase Inhibition in BRCA1/2-Proficient Cancer CellsBiomedicinesApril 14, 2025Danyang Zhou, Wei Liu, Yanyan Zhang, Chong Li(single-cell gel electrophoresis) was performed using the OxiSelect™ Comet Detection Kit (ENZO Life Science, Farmingdale, NY, USA, #ADI-900-166) according to the manufacturer’s instructions. Briefly, after treatment, cells were suspended in PBS

(4) CD44-targeted virus-mimicking nanomedicine eliminates cancer stem cells and mitigates chemoresistance in head and neck squamous cell carcinomaMater Today BioMarch 29, 2025Yiwen Chen, Zhen Qin, Yujia Wang, Baoxin Gu, Jing Wang, Yunfei Zheng, Yuting Niu, Lingfei JiaUSA). 2.14 Comet assays A single-cell gel electrophoresis (SCGE) comet assay was performed using a kit (ADI-900-166, Enzo Life Sciences, Farmingdale, NY, USA) to detect DNA damage. The cells were embedded in agarose,

(5) p53 enhances DNA repair and suppresses cytoplasmic chromatin fragments and inflammation in senescent cellsNat CommunMarch 5, 2025Karl N. Miller, Brightany Li, Hannah R. Pierce-Hoffman, Shreeya Patel, Xue Lei, Adarsh Rajesh, Marcos G. Teneche, Aaron P. Havas, Armin Gandhi, Carolina Cano Macip, et al.assay was carried out following manufacturer’s instruction for the neutral comet SCGE assay (Enzo, #ADI-900-166). Slides were placed flat in the dark at 4 C in the dark for 30 minutes. Slides were immersed in pre-chilled

(6) ATR inhibition increases reliance on PARP-mediated DNA repair revealing an improved therapeutic strategy for cervical cancerGynecologic OncologyDecember 1, 2024Sugantha Priya Elayapillai, Samrita Dogra, James Lausen, Madison Parker, Amy Kennedy, Doris M. Benbrook, Katherine M. Moxley, Bethany N. HannafonDNA strand breaks by using both alkaline and neutral conditions, using Enzo comet SCGE assay kit # ADI-900–166 following the manufacturer’s instructions. Briefly, SiHa and CaSki cells were pre-treated with ATRi

(7) Elevated 2-oxoglutarate antagonizes DNA damage responses in cholangiocarcinoma chemotherapy through regulating aspartate beta-hydroxylaseCancer LettersJanuary 1, 2024Katsuya Nagaoka, Xuewei Bai, Dan Liu, Kevin Cao, Joud Mulla, Chengcheng Ji, Hongze Chen, Muhammad Azhar Nisar, Amalia Bay, William Mueller, et al.comet assay was performed using the Comet SCGE assay kit following the manufacturer’s instruction (ADI-900-166, Enzo Life Science, Farmingdale, NY). In brief, cells at 1 x 105/ mL were combined with molten LMAgarose

(8) Pharmacological inhibition of MYC to mitigate chemoresistance in preclinical models of squamous cell carcinomaTheranosticsJanuary 1, 2024Liu S, Qin Z, Mao Y, Wang N, Zhang W, Wang Y, Chen Y, Jia L, Peng X.comet assays were performed using an SCGE assay Kit (Enzo Life Sciences, Farmingdale, NY, USA; Cat# ADI-900-166). Following treatment, the cells were mixed with low melting point agarose at a volume ratio of 1:50,

(9) Genistein and Procyanidin B2 Reduce Carcinogen-Induced Reactive Oxygen Species and DNA Damage through the Activation of Nrf2/ARE Cell Signaling in Bronchial Epithelial Cells In VitroInternational Journal of Molecular SciencesFebruary 12, 2023Tharindu L. Suraweera, J. P. Jose Merlin, Graham Dellaire, Zhaolin Xu, H. P. Vasantha Rupasingheantibodies were purchased from Thermo Fisher Scientific (Chelmsford, MA, USA). Comet SCGE assay kit (Cat: ADI-900-166) was purchased from Enzo (New York, NY, USA). Cellular DNA fragmentation ELISA kit ((Cat: 11585045001)

(10) Therapeutic Targeting of MYC in Head and Neck Squamous Cell CarcinomaOncoImmunologyDecember 31, 2022Shuo Liu, Zhen Qin, Yaqing Mao, Wenbo Zhang, Yujia Wang, Lingfei Jia, Xin Pengcomet assays were performed using an SCGE assay Kit (Enzo Life Sciences, Farmingdale, NY, USA; Cat# ADI-900-166). Following treatment, the cells were mixed with low melting point agarose at a volume ratio of 1:50,

(11) Determining the temporal, dose, and composition effects of nutritional substrates in an in vitro model of intrahepatocellular triglyceride accumulationPhysiological ReportsOctober 27, 2022Shilpa R. Nagarajan, Eloise Cross, Elspeth Johnson, Fabio Sanna, Lorna J. Daniels, David W. Ray, Leanne HodsonDNA damage by single cell gel electrophoresis was carried out using the Comet SCGE assay kit (Enzo #ADI‐900‐166). Cells were cultured in treatment as previously outlined for 7 days. Assay was carried out according

(12) Exportin 4 depletion leads to nuclear accumulation of a subset of circular RNAsNat CommunOctober 1, 2022Liang Chen et al.to the negative control. Comet assay Comet assays were conducted using Comet SCGE assay kit (Enzo, ADI-900-166). Cells were washed with Ca2+/Mg2+ free 1 × PBS (Biosharp, BL551A) and combined gently with molten LMAgarose

(13) Therapeutic Targeting of MYC in Head and Neck Squamous Cell CarcinomaOncoimmunologySeptember 30, 2022Shuo Liu, Zhen Qin, Yaqing Mao, Wenbo Zhang, Yujia Wang, Lingfei Jia, Xin Pengcomet assays were performed using an SCGE assay Kit (Enzo Life Sciences, Farmingdale, NY, USA; Cat# ADI-900-166). Following treatment, the cells were mixed with low melting point agarose at a volume ratio of 1:50,

(14) Combined Inactivation of CTPS1 and ATR Is Synthetically Lethal to MYC-Overexpressing Cancer CellsCancer researchMarch 15, 2022Sun Z, Zhang Z, Wang QQ, Liu JL.BAY-1895344 were gently scraped and subjected to alkaline comet assay with the Comet Assay Kit (Enzo ADI-900–166) according to the manufacturer's instructions. Comet tails were visualized by a fluorescent microscope

(15) SN-38 Sensitizes BRCA-Proficient Ovarian Cancers to PARP Inhibitors through Inhibiting Homologous Recombination RepairDisease markersJanuary 1, 2022Lin S, Tian J, He Q, Yang M, Chen Z, Belogurov AA Jr, Li X, Zhang F, Liu Y, Chen G.Winooski, VT, USA). 2.7. Comet Assay The comet assay was performed using an OxiSelect™ Comet Assay Kit (#ADI-900-166, ENZO Life Science) according to manufacturer's instructions. Briefly, cells were plated into a 6-well

(16) WRN helicase safeguards deprotected replication forks in BRCA2-mutated cancer cellsNature communicationsNovember 12, 2021Datta A, Biswas K, Sommers JA, Thompson H, Awate S, Nicolae CM, Thakar T, Moldovan GL, Shoemaker RH, Sharan SK, et al.neutral comet assay (single-cell gel electrophoresis) according to the Comet SCGE assay kit (Enzo, ADI-900-166) protocol. PEO4 and PEO1 cells (1 × 105 cells) were transfected with either control or MUS81 siRNA (80

(17) Targeting KDM4A epigenetically activates tumor-cell-intrinsic immunity by inducing DNA replication stressMolecular cellMay 20, 2021Zhang W, Liu W, Jia L, Chen D, Chang I, Lake M, Bentolila LA, Wang CY.of dsDNA was quantified using LAS X software. Comet SCGE assays Commercial comet assay kits (Cat#ADI-900–166, Enzo Life Sciences) were utilized in this study, and the assay was performed per the manufacturer’s

(18) Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage responseNature communicationsJanuary 20, 2021Wang W, Li J, Tan J, Wang M, Yang J, Zhang ZM, Li C, Basnakian AG, Tang HW, Perrimon N, et al.previously described39. Comet assay The Comet assays were performed using COMET Assay kit (Enzo, ADI-900-166) following the manufacturer’s instructions. 1 × 105 cells/ml were mixed with molten LM agarose at 37

(19) BMI1 Inhibition Eliminates Residual Cancer Stem Cells after PD1 Blockade and Activates Antitumor Immunity to Prevent Metastasis and RelapseCell stem cellAugust 6, 2020Jia L, Zhang W, Wang CY.Single cell gel electrophoresis comet assays were performed using the SCGE assay Kit (Enzo Life Sciences, Cat# ADI-900–166). Following the treatment, cells were mixed with low melting point agarose at a volume ratio of 1:50,

(20) Cyclin F Controls Cell-Cycle Transcriptional Outputs by Directing the Degradation of the Three Activator E2FsMolecular cellJune 20, 2019Clijsters L, Hoencamp C, Calis JJA, Marzio A, Handgraaf SM, Cuitino MC, Rosenberg BR, Leone G, Pagano M.comet assay was performed as indicated by the manufacturer’s instructions (ENZO Comet SCGE assay kit, #ADI-900–166). Briefly, U2OS cells were collected in PBS at ± 3*105 cell/mL concentration. Cells were diluted at

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10/25/2016Evaluation Of COMET SCGE Assay Kit Lufen ChangThe general aim of the research is to study the function of a gene in DNA damage repairs in cancer cells. The specific goals of the experiment were to examine a role of a gene in DNA damage repair in ... Read Review

Comet SCGE assay kit from Enzo Life Sciences, Inc.

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Specifications/Features

Enzo Life Sciences, Inc.

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Comet SCGE assay kit from Enzo Life Sciences, Inc.

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