MTT Cell Proliferation Assay Kit 10009365 from Cayman Chemical

Product Specs
ItemMTT Cell Proliferation Assay Kit
CompanyCayman Chemical
Price $219.00Supplier Page View Company Product Page
Catalog Number10009365
Quantity500 dtn
MethodAssay
Research AreaCell Biology, Cell Cycle, Cell Health & Viability, Toxicology & Xenobiotic Metabolism
Storage4
OriginUSA

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Neddylation relieves cytoskeletal tension to permit primary cilia formation during mouse decidualizationCell Communication and SignalingFebruary 18, 2026Chen Zhou, Yu-Ying He, Wen-Xu Yao, Yue Li, Bo Lidecidualizing stimuli for 24 h. Cell viability was determined using the MTT Cell Viability Assay Kit (10009365, Cayman Chemical) following the manufacturer’s protocol. Briefly, 10 µL of MTT reagent was added to

(2) Rational engineering of allosteric protein switches by in silico prediction of domain insertion sitesNat MethodsAugust 4, 2025Benedict Wolf, Pegi Shehu, Luca Brenker, Anna-Lisa von Bachmann, Ann-Sophie Kroell, Nicholas Southern, Stefan Holderbach, Joshua Eigenmann, Sabine Aschenbrenner, Jan Mathony, et al.were tested for cell viability using the MTT Cell Proliferation Assay Kit (Cayman Chemicals, cat. no. 10009365) according to the manufacturer’s protocol. For the microscopy assay, cells were transfected identically,

(3) Distinct Interactions of Cannabinol and Its Cytochrome P450-Generated Metabolites with Receptors and Sensory NeuronsJournal of Medicinal ChemistryJuly 10, 2025Debanjan Kundu, Luca Franchini, Hale S. Hasdemir, Elliot Lloyd, Jonathan Maturano, Katalin Rabl, Anna Nicole Denissiouk, Mark Schumacher, David Sarlah, Judith Hellman, et al.(Item No. 2808-39-1) and the kits for NO (Item No. 780001), LDH (Item No. 601170) and MTT (Item No. 10009365) assays were purchased from Cayman Chemicalsthe Mouse IL-6 uncoated ELISA kit from Thermo Fisher Scientific

(4) Acute exposure to groundwater contaminants mixture of nitrate, atrazine and imidacloprid impacts growth kinetics of poultry cecal microbiomes and significantly decreases Caco-2 cell viabilitybioRxivJune 23, 2025Chamia C. Chatman, Erica L.- W. Majumder[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to formazan using a MTT cell proliferation assay kit (Cayman Chemical, Item N0. 10009365). Confluent cells were incubated for 24 h incubation with the specified treatment. Caco-2 were exposed

(5) Microbial vitamin biosynthesis links gut microbiota dynamics to chemotherapy toxicitymBioJune 11, 2025Lars E. Hillege, Kai R. Trepka, Benjamin G. H. Guthrie, Xueyan Fu, Romy Aarnoutse, Maia R. Paymar, Christine Olson, Chen Zhang, Edwin Ortega, Lorenzo Ramirez, et al.IPB)",Illumina,20060059 Quant-iT Picogreen dsDNA Kit,Invitrogen,P7589 MTT Cell Proliferation Assay Kit,Cayman Chemical,10009365 Instruments,Instruments,Instruments Mini-Beadbeater-96,BioSpec,1001 Anaerobic chamber,Coy Laboratory

(6) Protocatechuic Acid Ameliorates Cisplatin-Induced Inflammation and Apoptosis in Mouse Proximal Tubular CellsInternational Journal of Molecular SciencesApril 26, 2025Karim M. Saad, Khaled Elmasry, Babak Baban, Man J. Livingston, Zheng Dong, Marwa E. Abdelmageed, Rania R. Abdelaziz, Ghada M. Suddek, Ahmed A. ElmarakbyDiphenyl Tetrazolium Bromide (MTT) Assay An MTT assay was performed using the kit from Cayman (Cat. No. 10009365) according to the manufacturer’s instructions. 4.3. Quantification of Barrier Function The barrier

(7) Biosynthetic pathway for leukotrienes is stimulated by lipopolysaccharide and cytokines in pig endometrial stromal cellsSci RepJanuary 22, 2025Barbara Jana, Aneta Andronowska, Jarosław Całka, Aleksandra Mówińska((3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) method using an MTT Cell Proliferation Kit (cat. no. 10009365, Cayman Chemical Co.), in accordance with the manufacturer’s instructions. Statistical analysis Only

(8) Kinin Receptors B1 and B2 Mediate Breast Cancer Cell Migration and Invasion by Activating the FAK-Src AxisInternational Journal of Molecular SciencesOctober 31, 2024Felipe González-Turén, Lorena Lobos-González, Alexander Riquelme-Herrera, Andrés Ibacache, Luis Meza Ulloa, Alexandra Droguett, Camila Alveal, Bastián Carrillo, Javiera Gutiérrez, Pamela Ehrenfeld, et al.period. Cell proliferation was evaluated using the MTT assay (Cell Proliferation Assay Kit, Item No. 10009365, Cayman Chemical, Ann Arbor, MI, USA), adding 10 μL of MTT reagent to each well and incubating the samples

(9) Dapagliflozin mitigates cellular stress and inflammation through PI3K/AKT pathway modulation in cardiomyocytes, aortic endothelial cells, and stem cell-derived β cellsCardiovascular DiabetologyOctober 29, 2024Fatmah R. Alsereidi, Zenith Khashim, Hezlin Marzook, Ahmed M. Al-Rawi, Tiana Salomon, Mahra K. Almansoori, Moustafa M. Madkour, Ahmed Mohamed Hamam, Mahmoud M. Ramadan, Quinn P. Peterson, et al.viability assessment The MTT assay was used to assess the cardiomyocyte viability (Cayman Chemical,10009365). The AC16 cardiomyocytes were incubated for three hours in DMEM containing 0.5 mg/mL MTT. The incubation

(10) Investigating the Suitability of Mare’s Milk-Derived Exosomes as Potential Drug CarriersBiomoleculesOctober 1, 2024Shynggys Sergazy, Sanzhar Zhetkenev, Zarina Shulgau, Laura Chulenbayeva, Yevgeniy Kamyshanskiy, Madiyar Nurgaziyev, Ayaulym Nurgozhina, Zhanel Mukhanbetzhanova, Kulzhan Berikkhanova, Alexander Gulyayev, et al.cells were treated with 1 × PBS. 2.4.2. Cell Viability To determine cell viability, an MTT assay kit (10009365, Cayman, Ann Arbor, MI, USA) was used, which is a colorimetric assay that measures cellular metabolic

(11) α7 nicotinic receptor activation mitigates herpes simplex virus type 1 infection in microglia cellsAntiviral ResearchAugust 1, 2024Shih-Heng Chen, Joanne C. Damborsky, Belinda C. Wilson, Rick D. Fannin, James M. Ward, Kevin E. Gerrish, Bo He, Negin P. Martin, Jerrel L. Yakel2006). 2.3. MTT assay MTT assay was performed using a commercially available kit (Cayman Chemical, Cat. #: 10009365) to evaluate the viability of BV2 cells following treatment with choline or PNU-282987. Cells were plated

(12) Effect of Arthrospira maxima Phycobiliproteins, Rosiglitazone, and 17β-Estradiol on Lipogenic and Inflammatory Gene Expression during 3T3-L1 Preadipocyte Cell DifferentiationInternational Journal of Molecular SciencesJuly 10, 2024Ruth Marina García-García, María Eugenia Jaramillo-FloresDMSO concentration per well ≤0.1%. The MTT used was the Cayman Chemical Cell Proliferation MTT Kit No. 10009365. Stock solutions of PBPs were prepared in differentiation medium, while ethanol was used for preparing

(13) A homogeneous time-resolved fluorescence screen to identify SIRT2 deacetylase and defatty-acylase inhibitorsPloS oneJanuary 1, 2024Yang J, Cassel J, Boyle BC, Oppong D, Ahn YH, Weiser BP.adding 100 μl of crystal dissolving solution for another 16 hour incubation (Cayman Chemical, catalog #10009365). The absorbance in each well was measured at 570 nm and was used to determine the cell viability by

(14) Triple negative breast cancer metastasis is hindered by a peptide antagonist of F11R/JAM‑A proteinCancer cell internationalAugust 11, 2023Bednarek R, Wojkowska DW, Braun M, Watala C, Salifu MO, Swiatkowska M, Babinska A.cell viability assay The assay was performed by MTT Cell Proliferation Assay Kit (Cayman Item no. 10009365). EA.hy926, HMEC-1 and MDA-MB-231 cells were seeded in culture media at a number of 1 × 103 cells per

(15) The Cytotoxicity of Carbon Nanotubes and Hydroxyapatite, and Graphene and Hydroxyapatite Nanocomposites against Breast Cancer CellsNanomaterials (Basel, Switzerland)January 30, 2023Nguyen T, Maniyar A, Sarkar M, Sarkar TR, Neelgund GM.of CNTs-HAP and GR-HAP was analyzed by MTT cell proliferation assay kit (Cayman Chemical Company, #10009365; Ann Arbor, MI, USA) with SUM-159 and MCF-7 cells using the manufacturer’s standard protocol [59]. The

(16) Vitamin D inhibits osteosarcoma by reprogramming nonsense-mediated RNA decay and SNAI2-mediated epithelial-to-mesenchymal transitionFrontiers in oncologyJanuary 1, 2023Capobianco E, McGaughey V, Seraphin G, Heckel J, Rieger S, Lisse TS.(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was performed according to the manufacturer´s recommendation (10009365; Cayman Chemical). After incubation with the MTT reagent, the cells and salt were solubilized using

(17) Discovery of 8-Hydroxyquinoline as a Histamine Receptor 2 Blocker ScaffoldACS Synthetic BiologyAugust 19, 2022Paola L. Marquez-Gomez, Nicholas S. Kruyer, Sara L. Eisen, Lily R. Torp, Rebecca L. Howie, Elizabeth V. Jones, Stefan France, Pamela Peralta-YahyaToxicity Assay Mammalian cell viability was assayed using an MTT Cell Proliferation Assay Kit (Cayman Chemical, 10009365). HEK293T cells were cultured in T75 flasks until reaching 70–90% confluence. Cells were trypsinized,

(18) Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells and its implication in therapy resistanceCell reportsJuly 19, 2022Islam R, Mishra J, Polavaram NS, Bhattacharya S, Hong Z, Bodas S, Sharma S, Bouska A, Gilbreath T, Said AM, et al.microscope. MTT assay MTT assay was performed according to manufacturer’s protocol (Cayman Chemical, 10009365). In brief, cells were seeded in a 96-well plate at a density of 5000 cells/well in 100 μL of culture

(19) Nuclear soluble cGAS senses double-stranded DNA virus infectionCommunications biologyMay 10, 2022Wu Y, Song K, Hao W, Li J, Wang L, Li S.with DMF, cisplatin, or HSV-1. Cell viability was determined by the MTT Assay Kit (Cayman Chemical, #10009365). Real-time PCR Total RNA was prepared using the E.Z.N.A. Total RNA Kit I (Omega Bio-Tek Inc, R6834).

(20) Pirfenidone increases IL-10 and improves acute pancreatitis in multiple clinically relevant murine modelsJCI insightJanuary 25, 2022Palathingal Bava E, George J, Tarique M, Iyer S, Sahay P, Gomez Aguilar B, Edwards DB, Giri B, Sethi V, Jain T, et al.used to assess macrophage viability at 24- and 72-hour incubation times (Cayman Chemical, item no. 10009365). Splenocytes culture. Spleens were harvested from C57BL/6J mice with L-arginine AP at peak of injury

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MTT Cell Proliferation Assay Kit

MTT Cell Proliferation Assay Kit

MTT Cell Proliferation Assay Kit from Cayman Chemical

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Cayman Chemical

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MTT Cell Proliferation Assay Kit from Cayman Chemical

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