Description
Cayman’s SIRT2 Direct Screening Assay Kit provides a convenient fluorometric method for screening SIRT2 modulators in a two-step reaction. In the first step, the SIRT2/SIRT3 peptide and NAD+ are incubated with human recombinant SIRT2. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be easily analyzed using an excitation wavelength of 360 nm and an emission wavelength of 465 nm