CellTiter 96 Non-Radioactive Cell Proliferation Assay G4000 from Promega

Product Specs
ItemCellTiter 96 Non-Radioactive Cell Proliferation Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG4000
Detection TargetCell Proliferation
Sample TypeLive Cells
Quantity1,000 assays
MethodColorimetric

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

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Website: http://www.promega.com

(1) MYC-bound enhancer RNAs in cis regulate gene transcription and tumorigenesisSci AdvApril 24, 2026Sihan Li, Zehua Wang, Phuong Nguyen, Yue Wang, Yueshan Zhao, Yifei Wang, Yuang Chen, Haozhe Huang, Xiaofei Wang, Yu Zhang, et al.time points was measured using the CellTiter 96 Non-Radioactive Cell Proliferation Assay Kit (Promega, G4100) following the manufacturer’s guidelines. The absorbance value was measured at 570 nm with a reference

(2) Deep-learning-based de novo discovery and design of therapeutics that reverse disease-associated transcriptional phenotypesCellMarch 17, 2026Jing Xing, Mingdian Tan, Dmitry Leshchiner, Mengying Sun, Mohamed Abdelgied, Li Huang, Shreya Paithankar, Katie Uhl, Rama Shankar, Erika Lisabeth, et al.Hep3B cells were assessed using the CellTiter 96 Aqueous One Solution Cell Proliferation assay (cat# G4100, Promega). Cells were seeded at 3×103 cells per well in 96-well plates and allowed to adhere overnight.

(3) Misregulated alternative splicing in endometriosis: a role for aberrant mRNA variants in endometriotic cell growthCell Death DiscoveryMarch 15, 2026Venkata Naga Goutham Davuluri, Michelle Dias, Roxanna Llinas, Kamath Neha, Pooja Popli, Shannon M. Hawkins, Chandrakant Tayade, Elise T. Courtois, Hari Krishna Yalamanchili, Ramakrishna KommaganiMTT assay MTT assays were performed using the CellTiter 96® Non-Radioactive Cell Proliferation Assay (Promega: G4000) according to the manufacturer’s instructions. Briefly, IHEEC cells were transfected as described above.

(4) Identification and Experimental Validation of Triosephosphate Isomerase 1 as a Functional Biomarker of SHetA2 Sensitivity in Ovarian CancerCellsJanuary 30, 2026Laura F. Mortan, Zitha Redempta Isingizwe, Doris Mangiaracina Benbrookstandard 96-well cell culture plates by Cell Titer 96 Non-Radioactive Cell Proliferation Assay (Promega #G4100, Madison, WI, USA). The siRNA transfection and subsequent viability assay was performed 3 times in triplicate. 2.8.

(5) ATGL suppresses ferroptosis in acute myeloid leukemia cells by modulating the CEBPα/SCD1 axis and induces gilteritinib resistanceCell Death & DiseaseJanuary 9, 2026Shiyi Yuan, Ying Zhou, Wenrui Xiao, Ning Liu, Ping Zhang, Ying Zhang, Jianchuan Deng, Liang Fang, Xi Zhang, Shifeng Louproliferation, apoptosis, and cell cycle assays Cell proliferation was assessed using Cell Counting Kit-8 (G4000, Promega, Madison, WI). The apoptosis rate was assessed using an Annexin V-FITC/PI apoptosis detection

(6) Eco-friendly fabrication of vanadium nanoparticles via Fusarium solani with dual antifungal and anticancer bioactivitiesRSC AdvancesJanuary 7, 2026Saman M. Mohammed, Haider M. Hamzahbromide] assay,53 employing Promega's CellTiter 96® AQueous One Solution Cell Proliferation assay kit (#G4000), in two human pancreatic cancer cell lines, T3M-4 and CD18/HPAF that obtained from ATCC (Manassas,

(7) Molecular and biophysical requirements for B cell receptor dependent enhancement of dengue virus infectionbioRxivJanuary 7, 2026Gaby Madrigal, Chad Gebo, Benjamin D. McElvany, Sean A. Diehl, Adam T. Waickmanformazan utilizing the CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) (Promega, cat. no. G4000). 1% Triton-X (Thermofisher, cat. no. A16046.AE) was used as a positive control for cell death. Data

(8) Anti‐fibrotic effects of lisinopril ( ACE inhibitor) and fasudil ( ROCK inhibitor) in combination for canine corneal fibrosis in vitroVeterinary OphthalmologyJanuary 1, 2026Brayden L. Routh, Ratnakar Tripathi, Elizabeth Giuliano, Payton Lujin, Prashant R. Sinha, Rajiv R. Mohanviability were analyzed with a Cell Titer 96®Non-Radioactive Cell viability Assay (MTT) (Promega, Cat. No. G4000) following the manufacturer’s instructions. CCFs were seeded in a 96-well plate at a density of 5 ×

(9) NMR and proteomic analysis of Cornus officinalis extract reveals abundance of 5-hydroxymethylfurfural and induced expression of Nrf2 and superoxide dismutase-2 in 1.1B4 human pancreatic β-cells and murine isletsMolecular and Cellular EndocrinologyJanuary 1, 2026Justin D. Fletcher, Hailey L. Maurer, Mark A. Eschenfelder, Benjamin R. Smith, Sama Nayakanti, Jennifer Guergues, Tiara Wolf, Stanley M. Stevens, Brant R. Burkhardtand then the respective treatments. After the initial treatments, the MTT assay (Promega, Item No. G4000) was performed. In brief, the MTT dye (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)

(10) Next-Generation MDMA Analogue SDMA: Pharmacological and Metabolic InsightsACS Chem NeurosciDecember 17, 2025Nina Kastner, Núria Nadal-Gratacós, Selina Hemmer, Leticia Alves da Silva, John L. McKee, Tamara Hell, Giulia Cicalese, Marion Holy, Fatemeh Kooti, Kathrin Jäntsch, et al.1–6). Cytotoxicity Assay Cytotoxicity assays were performed according to the manufacturer protocol (G4000, Promega, WI, USA) for HEK cells and described previously for PC12 cells (detailed information in the

(11) Residual Breast Cancer Cells Co-opt SOX5-driven Endochondral Ossification to Maintain DormancyCancer DiscoveryDecember 11, 2025Amulya Sreekumar, Eric Blankemeyer, Christopher J. Sterner, Tien-Chi Pan, Dhruv K. Pant, Sarah Acolatse, Hamza Turkistani, George K. Belka, Anupma Nayak, Sean D. Carlin, et al.manufacturer’s instructions using the Cell Titer 96 Non-Radioactive Cell Proliferation Assay (Promega, Cat. #G4000) in 96-well plates. All cells were verified to be free of Mycoplasma contamination. Plasmids and lentiviral

(12) Targeting CXCR6 Disrupts β-Catenin Signaling and Enhances Sorafenib Response in Hepatocellular CarcinomaCancers (Basel)November 28, 2025Morgan Reeves, Anastasia Chambers, Abhishek Shrestha, Sergio Duarte, Ali Zarrinpar, Siobhan Malany, Satyamaheshwar Peddibhotla#284461-73-0) for 24–72 h, after which cell viability was assessed by MTT assay (PROMEGA, Madison, WI, USA #G4100) according to the manufacturer’s instructions. Absorbance was measured at 570 nm using a microplate

(13) Targeting fibroblast derived thrombospondin 2 disrupts an immune-exclusionary environment at the tumor front in colorectal cancerNature CommunicationsNovember 23, 2025Kosuke Iwane, Yuki Nakanishi, Yu Muta, Jiayu Chen, Kento Yasumura, Mayuki Omatsu, Naoki Aoyama, Munehiro Ikeda, Yoko Masui, Liyang Cai, et al.on a 96-well plate at a density of 500 cells per well and incubated. CellTiter 96 reagent (Promega; G4000) was added to the medium at 20% of the total volume. The plate was then incubated at 37 °C for 1 h.

(14) ALKBH1 Drives Tumorigenesis and Drug Resistance via tRNA-decoding Reprogramming and Codon-biased TranslationCancer DiscoveryNovember 3, 2025Chao Shen, Yuan Che, Keren Zhou, Kitty Wang, Wei Li, Diyuan Xue, Tong Wu, Lu Yang, Meiling Chen, Yue Sheng, et al.S3. Cell viability and apoptosis assays Cell viability was assessed using the MTT assay (Promega, G4100) following the manufacturer’s instructions. Briefly, cells were seeded into 96-well plates in triplicates

(15) Functional variants of CFAP410 affect the DNA damage response leading to motor neuron degeneration – Implications for ALSiScienceSeptember 19, 2025Ross Ferguson, Vasanta Subramanianassays,Critical commercial assays ,, CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT),Promega,Cat# G4000 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, R1

(16) Probing the metabolic regulator and candidate tumor suppressor ALDH1L1 as a target for non-small cell lung cancer gene therapyMolecular Therapy OncologySeptember 18, 2025Amira A. Abdellatef, Blake R. Rushing, Susan Sumner, Natalia I. Krupenko, Sergey A. KrupenkoLT005). Cell proliferation assay Cell viability was assessed using MTT cell proliferation assay (Promega, G4000). Cells were plated at a density of 5 × 103 cells/well in a 96-well format, MTT was added at specified

(17) Imeglimin suppresses glucagon secretion and induces a loss of α cell identityCell Reports MedicineAugust 19, 2025Takahiro Tsuno, Jinghe Li, Kuniyuki Nishiyama, Yuka Imamura Kawasawa, Ryota Inoue, Esther Ong Yajima, Akira Nishiyama, Shigeharu G. Yabe, Tatsuya Kin, Hitoshi Okochi, et al.viability was determined using the CellTiter 96 Non-Radioactive Cell Proliferation Assay (Promega, G4100) according to the manufacturer’s instructions. NAD+/NADH levels were quantified using the NAD+/NADH Glo assay

(18) Repurposing the DNA Labeling Agent EdU for Therapy against Heterogeneous Patient GlioblastomaMolecular Cancer TherapeuticsAugust 1, 2025Humeyra Kaanoglu, Adebimpe Adefolaju, Casey Fraley, Mariah Shobande, Rajaneekar Dasari, Breanna Mann, Noah Bell, Stephen T. Keir, Dominique Higgins, David E. Kram, et al.EdU or TMZ and incubated for 72 h. Then, MTT dye was added from Promega MTT Kit (Promega, catalog #: G4000) and cells were incubated for 4 h. Following this incubation, Solubilization Solution from the Promega

(19) Mortalin and PINK1/Parkin‐Mediated Mitophagy Represent Ovarian Cancer‐Selective Targets for Drug DevelopmentAdvanced ScienceJuly 20, 2025Vishal Chandra, Justin Garland, Rajani Rai, Donghua Zhao, Charuksha Walgama, Sadagopan Krishnan, Andrew T. Long, Tongzu Liu, Laura Adhikari, Doris M. Benbrookpurchased from Sigma (St Louis, MO, USA). Cell Titer 96 Non‐Radioactive Cell Proliferation Assay (MTT) (#G4100) was purchased from Promega (Madison, WI, USA). Transfecting reagent HiPerfect (#301704) and lipofectamine

(20) Integrated Nanopore and short-read RNA sequencing identifies dysregulation of METTL3- m6A modifications in endocrine therapy- sensitive and resistant breast cancer cellsFunct Integr GenomicsJuly 9, 2025Belinda J. Petri, Kellianne M. Piell, Bailey L. Avila-Valdes, Christian G. Stanley, Laura J. Winkler, Johnny Tyler Brown, Rye Ulett, Gabriela Sanchez, Julia H. Chariker, Eric C. Rouchka, et al.treatment with DMSO (1/1000th volume) or 1 µM STM2457. MTT assays used Promega CellTiter 96® (cat. no. G4000, Promega, Madison, WI, USA) with quadruplicate replicates within each experiment. IC50 values were calculated

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Promega's CellTiter 96 Non-Radioactive Cell Proliferation Assay
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CellTiter 96 Non-Radioactive Cell Proliferation Assay

CellTiter 96 Non-Radioactive Cell Proliferation Assay from Promega

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Promega's CellTiter 96 Non-Radioactive Cell Proliferation Assay

CellTiter 96 Non-Radioactive Cell Proliferation Assay from Promega

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