Dual-Glo Luciferase Assay System E2920 from Promega

Product Specs
ItemDual-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2920
ReactivityMammalian
Detection TargetLuciferase
SpeciesMammalian
Sample TypeMammalian cell culture media
MethodLuciferase
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Thioredoxin reductase-1 facilitates cellular plasticity and stemness mediated by TGF-β1 in non-small cell lung cancerCancer Biol TherDecember 31, 2026Yaw-Dong Lang, Shin-Yuan Gu, Jou-Ho Shih, Chun-Jung Lin, Hua-Hsin Chiang, Tsai-Yu Lin, Yu-Ching Lee, Keng-Chang Tsai, Jui-Tin Chang, Cheng-Ying Chu, et al.activities of firefly and Renilla were measured using the Dual-Glo® Luciferase Assay System (Promega, E2920, USA) based on the manufacturer’s instructions. A549 cells were infected with a luciferase-expressing

(2) The mRNA architecture of the translation termination site primes programmed stop codon readthrough events in DrosophilaRNA BiolDecember 31, 2026Laveena Kansara, Georg Wolfstetter, Daniel Wintermayr, Ioannis Alexopoulos, Alejandra Escos, Marc Friedländer, Ylva Engströmassay Luciferase assays were performed using the Dual-Glo® Luciferase Reporter Assay System (Promega, #E2920) following the manufacturer’s instructions. Luminescence was measured with an EnSpire 2300 Multi-mode

(3) Gm26550 Modulates Learning and Memory by Increasing IGF1 mRNA Expression and Stability in Nrf2-/- MiceAdv Sci (Weinh)September 27, 2026Hongfang Wang, Ziyao Wang, Dongyue Zuo, Zhaowen Su, Bowen Song, Jiamin Gao, Yizhou Zhang, Ruiting Zhao, Linjie Sun, Mengdi Li, et al.Subsequently, luciferase activity was determined using the Dual‐Luciferase Reporter System kit (Cat#: E2920, Promega Corporation) in strict accordance with the manufacturer's instructions. Relative fluorescence

(4) LAPTM5 Downregulation-Driven VAMP8 Phosphorylation Impairs Autophagosome-Lysosome Fusion and Aggravates Septic Acute Lung InjuryAdv Sci (Weinh)September 27, 2026Lang Jiang, Qilan Li, Hao Liu, Xuehui Gao, Hongyu Nie, Lang Chen, Mei He, Xuefeng Li, Xiangming Fang, Jiqian Xu, et al.incubation, luciferase activity was measured using the Dual‐Luciferase Reporter Assay System (Promega, E2920). Normalized firefly luciferase activity relative to Renilla luciferase is presented as relative luciferase

(5) Mechanistic Insights into Regulation of the Aryl Hydrocarbon Receptor Expression in Mouse and Human Hepatoma CellsInt J Mol SciSeptember 20, 2026Yiyuan Wang, William K. ChanLuciferase activity was measured 48 h after transfection using the Dual-Glo® Luciferase assay system (E2920, Promega). Firefly luciferase activity was normalized to Renilla luciferase activity to determine relative

(6) Ultrasound controllable and antigen-independent killing via cis-activating CAR T cellsMolecular Therapy OncologySeptember 17, 2026Linshan Zhu, Jiaxin Cui, Chi-Wei Man, Anthony Mouchawar, Xi Yu, Yixuan Huang, Yiqian Wu, Tianze Guo, Hongquan Xu, Longwei Liu, et al.for 24 h. Bioluminescence measurements were taken using the Dual Glo Luciferase Assay kit (Promega, E2920). Cytotoxicity was calculated by taking the percent difference in luminescence of T cells-cultured wells

(7) NAT10 is critical to block RNA sensing-induced IFN-β transactivation in viral infectionmBioSeptember 9, 2026Youngmin Park, Yan Liu, Jinshan He, Suha Eleya, Zhenyu Wu, Guillaume Nicolas Fiches, Dawei Zhou, Emily G. Watters, Zhixian M. He, Kateepe Arachchige Sudeera Nuwan Shanaka, et al.transfected cells, they were treated with poly(I:C) for another 24 h. The Dual-Glo luciferase system (Cat. #E2920, Promega) was used for quantification of firefly and renilla luminescence. Luminescence was measured

(8) Functional Dissection of TP63 Dual-Promoter Regulatory Elements Reveals Context-Dependent Responsiveness to FOXA2, NKX2-1, SOX9, and TP63-Related InputsCurr Issues Mol BiolAugust 30, 2026Yijian Lin, Xiaoshan Su, Dachun Wang, Yiming Zengand Renilla luciferase activities were measured using the Dual-Glo Luciferase Assay system (Promega, E2920, Madison, WI, USA) following the manufacturer’s protocol. Specifically, 16 h post-transfection, 75 µL

(9) Guanylate-binding protein 2 (GBP2) drives pancreatic cancer cell proliferation by promoting cell cycle progression via the SP1/SKP2/P27 axisJournal of Translational MedicineAugust 17, 2026Shilin Fang, Haihang Nie, Jingkai Zhou, Yongxi Zhang, Xiaoping Liu, Jianchun Guo, Yuntian Hong, Yang Ding, Yumei Ning, Fan Wang, et al.firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter Assay System (E2920, Promega, USA) according to the manufacturer’s instructions. Co-immunoprecipitation (Co-IP) For Co-IP,

(10) Kuanxiong aerosol protects against coronary microvascular dysfunction through improving angiogenesis via HIF-1α-ITGAX axisFront PharmacolAugust 13, 2026Yuanqing Jiang, Kai Qian, Beibo Li, Mengmeng Xu, Pengfei OuYang, Yangyang Zhu, Jiaxu Wu, Xiaoying Shi, Yi Luo, Yao Xie, et al.Renilla luciferase activities were measured using the dual luciferase reporter assay kit (Promega, USA, #E2920). 2.25 Tube formation assay After treatment, HCMECs (7 × 104 per well) were seeded onto Matrigel-coated

(11) METTL14-dependent N6-methyladenosine modification of FGF16 participates in IGFBP5 deficiency-enhanced angiogenesisExperimental & Molecular MedicineAugust 4, 2026Fei Song, Yu Hu, Yi-Xiang Hong, Shan-Shan Zhao, Hui-Zhu Huang, Yutian Wang, Le Zhang, Wei-Yin Wu, Yan Wang, Gang LishRNA. The FGF16 translational efficiency was assessed using a Dual-Glo Luciferase Assay System (Cat#: E2920, Promega) 48 h post-transfection, following the manufacturer’s instructions. After 48 h, cells were

(12) Membrane lipid composition and rapid endocytic retrieval modulate Wingless secretionThe EMBO JournalAugust 3, 2026Ines Alvarez-Rodrigo, David Willnow, Cyrille Alexandre, Claire Lebarbenchon, Annabel Borg, Heather Findlay, Maya Hoad Leftley, Ikram Arahouan, Yuguang Zhao, Florencia di Pietro, et al.#17-0948-01 TransIT transfection reagent,Sigma,Cat #MIR230 Dual-Glo Luciferase Reporter Assay System,Promega,Cat #E2920 MES buffer,Invitrogen,Cat #13266499 1:1 Clarity Max Western Blot ECL Substrate,Bio-Rad,Cat #1705062 ImmersolT518

(13) Modification of the Transcription Factor FOXL2 at Serines 101 and 107 Disables DNA Binding, Leads to Nucleolar Relocalization, and Rewires Granulosa‐Cell ProgramsFASEB JAugust 1, 2026Ludovic Mousseron, Despoina Chousianiti, Francis Poulat, Bérangère Legois, Reiner A. Veitia, Anne‐Laure Todeschini11668027). After 48 h, cells were lyzed and analyzed with the Dual‐Glo Luciferase Assay System (Promega, E2920) according to the manufacturer's instructions. Relative luciferase activity was determined as the ratio

(14) Ahcy Acts as an Effector of Hnf4a-Driven Super-Enhancer Activation to Alleviate MASLD During Intermittent FastingAdv Sci (Weinh)July 27, 2026Huafeng Chen, Shilin Zhang, Xiaojie Deng, Wenqiang Xie, Fen Xu, Jie Shen, Hua LiangSamples were collected for detection 48 h after transfection. The Dual‐GLO Luciferase Assay System (E2920, Promega, USA) was used to measure luciferase activity. Dual‐Glo Luciferase Reagent and Dual‐Glo Stop

(15) CCL21/CCR7 axis regulates VEGF-D m6A modification to drive lymphangiogenesis and lymphatic metastasis in gallbladder cancerHum CellJuly 24, 2026Zhenheng Wu, Xinran Cai, Lei Jiang, Bin Lin, Wei Pan, Shengzhe Lin, Yanling Chen, Haijie Hongfirefly luciferase (F-luc) activity was measured using the Dual-Luciferase Reporter Assay System (Promega E2920, USA) according to the manufacturer’s instructions. Renilla luciferase (R-luc) activity was used as

(16) ATG9A is an essential host factor for parechovirus RNA replicationJournal of VirologyJuly 21, 2026You Li, Lorellin A. Durnell-Bettis, Fahmida Alam, Adriana E. Golding, Juan S. Bonifacino, Matthew R. VogtTechnology, #325). FLuc and Rluc assays were carried out with the Dual-Glo Luciferase Assay System (Promega, E2920). Luminescence was measured using a BioTek Synergy II multi-mode plate reader (Agilent Technologies). Transfections Viral

(17) ZBTB11 Promotes Breast Cancer Progression by Activating FBXO28-Mediated MST1 Degradation and Suppressing Hippo SignalingAdv Sci (Weinh)July 20, 2026An Xu, Xiang-Nan Xu, Xiao Huang, Zhou Luo, Chun-Lian Li, Yang Du, Cheng Yan, Xiao-Jie Yu, Jian-Wen Wang, Long-Di Yao, et al.lysed, and luciferase activities were measured using the Dual‐Glo Luciferase Assay System (Promega, E2920) on a multimode microplate reader (Molecular Devices, M3). Firefly luciferase activity was normalized

(18) STEMIN- and YAP5SA-induced exosomes prevent cardiomyocyte apoptosisiScienceJuly 17, 2026Adeniyi Adeleye, Siyu Xiao, Ilkin Tetik Altintop, Azeez Muili, Micah Castillo, Preethi Gunaratne, Kacie Waiters, Tasneem Bawa-Khalfe, Bradley K. McConnell, Robert J. Schwartz#11320033 Passive Lysis 5X Buffer,Promega,Cat #E1910 Dual-Glo® Luciferase Assay System,Promega,Cat #E2920 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, AC16

(19) The maternal KRAB-ZFP ZFPOBI1 reveals structural constraints governing ERV transcriptional co-option in mouse oocytesFront Cell Dev BiolJuly 14, 2026Andrina J. Stäubli, Yan Huang, Louise J. H. Rasmussen, Freja Mosegaard Hansen, Hui Mun Loh, Michael J. Claxton, Daniel M. Messerschmidtwere lysed and luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega, E2920) according to the manufacturer’s protocol. Experiments were performed in triplicates and repeated at

(20) Nuclear condensates formed by truncated mutant NEK1s impede ribosomal RNA biogenesis and drive motor dysfunctionNature CommunicationsJuly 13, 2026Yixin Wang, Wen Hu, Rui Huang, Fanmin Wu, Haopeng Su, Jianye Zang, Xiaolei Huang, Yanli Liu, Haigang Ren, Jian Li, et al.truncated mutant NEK1s. After 36 h of transfection, the cells were lysed in passive lysis buffer (Promega, E2920), and the expression of Renilla luciferase was determined using dual luciferase reporter assay kit (Promega,

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12/27/2021Great and Efficient Luciferase System Wei Yang SitThe convenient "add-mix-measure" protocol generates signals from cells that have not been preconditioned or prelysed.

Dual-Glo Luciferase Assay System

Dual-Glo Luciferase Assay System from Promega

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Dual-Glo Luciferase Assay System from Promega

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