Mouse IFN-γ Secretion Assay – Detection Kit (PE) 130-090-516 from Miltenyi Biotec

(1) Chimeric MHC class I- and II-restricted non-self epitopes broaden antitumor T cell reactionsJ Exp MedFebruary 2, 2026Rongsheng Zhang, Rong Ma, Merrin M.L. Leong, Ian R. Watson, Kei Iida, Tomonori Yaguchi, Fumihiko Matsuda, Tasuku Honjo, Kenji Chamotocells was measured using a mouse IFN-γ secretion assay according to the manufacturer’s instructions (130-090-516; Miltenyi Biotec), and peptide synthesis was performed using GenScript. ELISpot assay 6-wk-old female

(2) Cbl-b mitigates the responsiveness of naive CD8 + T cells that experience extensive tonic T cell receptor signalingScience SignalingFebruary 6, 2024Joel Eggert, Wendy M. Zinzow-Kramer, Yuesong Hu, Elizabeth M. Kolawole, Yuan-Li Tsai, Arthur Weiss, Brian D. Evavold, Khalid Salaita, Christopher D. Scharer, Byron B. Au-YeungIL-2-secreting OT-I cells were co-labeled using the IFNγ Secretion Assay Kit (Miltenyi Biotech, catalog #130-090-516) and the IL-2 Secretion Assay Kit (Miltenyi Biotech, catalog #130-090-987) after 16 hours of stimulation.

(3) Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxivFebruary 1, 2023Joel Eggert, Wendy M. Zinzow-Kramer, Yuesong Hu, Yuan-Li Tsai, Arthur Weiss, Khalid Salaita, Christopher D. Scharer, Byron B. Au-YeungIL-2-secreting OT-I cells were co-labeled using the IFNγ Secretion Assay Kit (Miltenyi Biotech, catalog #130–090-516) and the IL-2 Secretion Assay Kit (Miltenyi Biotech, catalog #130–090-987) after 16 hours of stimulation.

(4) IFN-γ(+) cytotoxic CD4(+) T lymphocytes are involved in the pathogenesis of colitis induced by IL-23 and the food colorant Red 40Cellular & molecular immunologyJuly 1, 2022Chen L, He Z, Reis BS, Gelles JD, Chipuk JE, Ting AT, Spicer JA, Trapani JA, Furtado GC, Lira SA.leukocytes from R23FR mouse ceca were incubated with a mouse IFN-γ catch reagent (Miltenyi Biotec, 130-090-516) for 5 min on ice. The cells were then incubated at a concentration of 1 × 105 cells/ml in warmed medium

(5) Long-peptide vaccination with driver gene mutations in p53 and Kras induces cancer mutation-specific effector as well as regulatory T cell responsesOncoimmunologyJanuary 1, 2018Quandt J, Schlude C, Bartoschek M, Will R, Cid-Arregui A, Schölch S, Reissfelder C, Weitz J, Schneider M, Wiemann S, et al.Mouse IL-2 Secretion Assay Detection Kit (APC*) 130–090-987 Mouse IFN-γ Secretion Assay Detection Kit (PE) 130–090-516) were downscaled to a 96-well plate format and carried out in triplicates for each tested peptide. 7.5

(6) IL-17 Augments B Cell Activation in Ocular Surface AutoimmunityJournal of immunology (Baltimore, Md. : 1950)November 1, 2016Subbarayal B, Chauhan SK, Di Zazzo A, Dana R.

(7) CD117+ Dendritic and Mast Cells Are Dependent on RasGRP4 to Function as Accessory Cells for Optimal Natural Killer Cell-Mediated Responses to LipopolysaccharidePloS oneJanuary 1, 2016Zhou S, Tanaka K, O'Keeffe M, Qi M, El-Assaad F, Weaver JC, Chen G, Weatherall C, Wang Y, Giannakopoulos B, et al.

(8) Regulated expression of a tumor-associated antigen reveals multiple levels of T cell tolerance in a mouse model of lung cancernihpaNovember 15, 2008Ann F. Cheung, Michel J.P. DuPage, H. Katie Dong, Jianzhu Chen, Tyler Jackssecreted IFN-γ capture/detection was performed with Mouse IFN-γ Secretion Assay Detection kit (order#130-090-516) from Miltenyi Biotec, according to manufacturers’ instructions. Biotinylated 1B2 monoclonal antibody

(9) Regulated Expression of a Tumor-Associated Antigen Reveals Multiple Levels of T-Cell Tolerance in a Mouse Model of Lung CancerCancer ResearchNovember 15, 2008Ann F. Cheung, Michel J.P. DuPage, H. Katie Dong, Jianzhu Chen, Tyler Jackssecreted IFN-γ capture/detection was performed with Mouse IFN-γ Secretion Assay Detection kit (order#130-090-516) from Miltenyi Biotec, according to manufacturers’ instructions. Biotinylated 1B2 monoclonal antibody

(10) Evaluation of bystander recruitment and cytotoxic functions of the IFN-? producing alloreactive CD8 + T cells in miceImmunology LettersOctober 17, 2004Sadi Köksoy, Lawrence E. Mathes

(11) T cytotoxic-1 CD8+ T cells are effector cells against pneumocystis in miceJournal of immunology (Baltimore, Md. : 1950)January 15, 2004McAllister F, Steele C, Zheng M, Young E, Shellito JE, Marrero L, Kolls JK.

(12) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritisJournal of immunology (Baltimore, Md. : 1950)August 15, 2003Yang JQ, Singh AK, Wilson MT, Satoh M, Stanic AK, Park JJ, Hong S, Gadola SD, Mizutani A, Kakumanu SR, et al.

(13) Protracted protection to Plasmodium berghei malaria is linked to functionally and phenotypically heterogeneous liver memory CD8+ T cellsJournal of immunology (Baltimore, Md. : 1950)August 15, 2003Berenzon D, Schwenk RJ, Letellier L, Guebre-Xabier M, Williams J, Krzych U.

(14) Distinct lineages of T(H)1 cells have differential capacities for memory cell generation in vivoNature immunologySeptember 1, 2002Wu CY, Kirman JR, Rotte MJ, Davey DF, Perfetto SP, Rhee EG, Freidag BL, Hill BJ, Douek DC, Seder RA.

(15) Adoptive tumor therapy with T lymphocytes enriched through an IFN-gamma capture assayNature medicineOctober 1, 2001Becker C, Pohla H, Frankenberger B, Schüler T, Assenmacher M, Schendel DJ, Blankenstein T.

(16) Cutting edge: culture with high doses of viral peptide induces previously unprimed CD8(+) T cells to produce cytokineJournal of immunology (Baltimore, Md. : 1950)September 1, 2001Riberdy JM, Zirkel A, Surman S, Hurwitz JL, Doherty PC.

(17) Interferon gamma stabilizes the T helper cell type 1 phenotypeThe Journal of experimental medicineJuly 16, 2001Zhang Y, Apilado R, Coleman J, Ben-Sasson S, Tsang S, Hu-Li J, Paul WE, Huang H.

(18) Critical contribution of IFN-gamma and NK cells, but not perforin-mediated cytotoxicity, to anti-metastatic effect of alpha-galactosylceramideEuropean journal of immunologyJune 1, 2001Hayakawa Y, Takeda K, Yagita H, Kakuta S, Iwakura Y, Van Kaer L, Saiki I, Okumura K.

(19) Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytesEuropean journal of immunologyMay 1, 1998Assenmacher M, Löhning M, Scheffold A, Manz RA, Schmitz J, Radbruch A.

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Mouse IFN-γ Secretion Assay – Detection Kit (PE) from Miltenyi Biotec

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Mouse IFN-γ Secretion Assay – Detection Kit (PE) from Miltenyi Biotec

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