L-Lactate Assay Kit (Colorimetric) ab65331 from Abcam

Product Specs
ItemL-Lactate Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65331
MethodFuncS
Quantity100 Test
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Assay Time0h 30m
SensitivityEqual to 0.02 mM
Detection Range0.02 - 20 mM
Research AreaMetabolism

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Hypertension Drives Protein Lactylation and Vascular Dysfunction in Skeletal MuscleHypertensionApril 22, 2026Milene T. Fontes, Paul Townsend, Landon Butler, Juliana M. Parente, Fênix A. Araujo, Tiago J. Costa, Gisele F. Bomfim, Laena Pernomian, Wenbin Tan, Brandon N. VanderVeen, et al.were collected, and lactate concentration was measured using a quantitative lactate assay kit (Abcam; #ab65331 for muscle; #ab65330 for plasma). Muscle samples were deproteinized using the Deproteinizing Sample Preparation Kit-TCA

(2) ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinomaCell DiscovApril 21, 2026Lijie Han, Lina Gan, Balázs Schäfer, Dillon M. Voss, Mathias Danielsen, Ondrej Kostov, Jia Liu, Ting Wang, Marvin H. Caruthers, Hao Chen, et al.kit (ab83432; Abcam), and lactic acid production was measured with a L-Lactate Assay Kit (ab65330 and ab65331; Abcam) in accordance with the manufacturer’s instructions. Cells were either transfected with 50 nM

(3) Icariin improves metabolic response to exercise by promoting TFEB-dependent mitochondrial clearance and metabolic reprogramming in C57BL/6 mice and C2C12 myotubesFront NutrApril 13, 2026Zhengyuan Liu, He Hu, Liang Zhenglactate concentration in the cell culture medium was measured using a commercial Lactate Assay Kit (AB65331, Abcam) according to the manufacturer's instructions. 2.5.2 LDH activity assay Intracellular LDH activity

(4) Protein phosphatase 2A orchestrates hematopoietic fate determination via modulation of lactate metabolismHaematologicaApril 1, 2026Can Liu, Yao Meng, Heng Chen, Siqi Bi, Ye Tian, Zhihua Yin, Guanhua Li, Wutao Chen, Li Wu, You Wang, et al.software. The levels of L-lactate in the cell lysate were quantified using the L-Lactate Assay kit (ab65331, Abcam). Quantification the enzymatic activities of histone deacetylases Overexpressed HDAC1-Flag

(5) BDNF facilitates ectopic endometrial stromal cell growth, invasion, migration and glycolysis in endometriosis via upregulating GLUT1CytojournalMarch 21, 2026Yapeng Han, Yang Yu, Yaguang Han, Wei Guan, Jieming Li, Boyi Feng, Chang Sun, Hong Ying Kuangratio were evaluated in ectopic ESCs using the glucose assay kit (ab65333, Abcam), L-Lactate assay kit (ab65331, Abcam), and ADP/ATP ratio assay kit (ab65313, Abcam), respectively. Co-immunoprecipitation (Co-IP)

(6) MALAT1 Regulates Human Macrophage Metabolism by Interacting with HADHBiScienceMarch 20, 2026Yuxiang Liu, Yukiteru Nakayama, Junichi Sugita, Tsukasa Oshima, Kunihito Kani, Atsushi Kobayashi, Naoto Setoguchi, Yoshiko Iwai, Ichiro Manabe, Katsuhito Fujiu37612 NEBNext Ultra II RNA Library Prep Kit,NEB,Cat# E7770L L-lactate Assay Kit (Colorimetric),Abcam,Cat# ab65331 Deproteinizing Sample Preparation Kit-TCA,Abcam,Cat# ab204708 MycoFluor Mycoplasma Detection Kit,Thermo Fisher

(7) ATAD2 drives immunotherapy resistance by promoting lactic acid-mediated CD8+ T cell dysfunction in lung adenocarcinomaFrontiers in ImmunologyMarch 19, 2026Wanfeng Gao, Jialei Xu, Yue Li, Jingchang Zhang, Chenghao Ma, Junfeng Chen, Jiajing Chencells, as well as in their corresponding tumor tissues, were measured with a Lactate Assay Kit (Abcam, ab65331) according to the manufacturer’s protocol. In vivo animal studies C57BL/6 mice were purchased from

(8) Aerobic glycolysis drives differentiation of unilocular adipocytesJournal of Lipid ResearchMarch 17, 2026Alice Maestri, Min Cai, Ruby Schipper, Julia Backman, Alana Vannay, Anneli Olsson, Ewa Ehrenborg, Roland Nilsson, Carolina E. Hagbergfrom media collected on day 5, 10, 15, 20, 25 and 30 using the L-Lactate Assay Kit (Colorimetric) (ab65331, Abcam), following the manufacturer’s instructions. Basal lactate levels were determined from fresh

(9) PKM2 phosphorylation by c-SRC activates glycolysis and metastasis with the stimulation of tumor-associated macrophagesCell Communication and SignalingMarch 12, 2026Da Song, Haijie Li, Jialu Xu, Wenli Zhan, Yaqi Chen, Xuelai Luo, Guihua Wang, Xingrui Li, Junbo Hu, Jingqin Lanincubated for another 6 h. The lactate content in the cultured medium was measured by L-Lactate Assay Kit (ab65331, Abcam). Statistical analyses All statistical analyses were conducted using GraphPad Prism 8.0. For

(10) KDM5B-driven glucose metabolic reprogramming promotes enzalutamide resistance in prostate cancer via the lactate/hnRNPA1 lactylation/AR-V7 axisMol CancerMarch 5, 2026Rui Sun, Yong Huang, Hao He, Qiuchen Li, Linfeng Wang, Gaojie Zhang, Ziling Wei, Yang Cao, Jing Li, Xianmin Wang, et al.levels in serum, cancer cells, and tumor tissues were measured using the L-Lactate Assay Kit (Cat. ab65331, Abcam, UK). Serum from patients was extracted from the supernatant of clotted whole blood by centrifuging

(11) Assessment of the angiogenic potential of xenografted tumors by biomedical imaging techniquesBiomedical Optics ExpressMarch 1, 2026Irina Druzhkova, Anna Orlova, Pavel Subochev, Alexey Kurnikov, Anna Glyavina, Alina Isakova, Ekaterina Kukovyakina, Ekaterina Plotnikova, Marine Gasparian, Anastasia Komarova, et al.deproteinization kit (ab204708, Abcam, USA). Samples were analyzed using a colorimetric lactate assay kit (ab65331, Abcam, USA) and glucose assay kit (ab65333, Abcam, USA) according to the manufacture’s protocol. The

(12) SOD1 lactylation impair its enzymatic activity by conformational change to aggravate intervertebral disc degenerationNature CommunicationsFebruary 28, 2026Yuyao Zhang, Yu Zhai, Chao Liu, Minghang Chen, Yang Zhang, Zhiqun Bian, Xian Chang, Zhilei Hu, Jianmin Li, Chao Zhang, et al.concentration of L-lactate content in NP tissue and NPCs was assessed using a L-lactate assay kit (ab65331, Abcam). Briefly, NPCs or milled NP tissues were mixed with assay kit buffer while a gradient concentration

(13) Disrupted energy metabolism is associated with retinal ganglion cell degeneration in autosomal dominant optic atrophyScience AdvancesFebruary 20, 2026Eugene Yu-Chuan Kang, Yun-Ju Tseng, Wei-Hao Peng, Hui-Chuan Hung, Pei-Hsuan Lin, Katrina P. Montales, Emmet Sherman, John Peregrin, Ethan Hunghsi Wang, Chunya Kang, et al.mouse retinas The levels of lactate in the retinas were measured using a commercially available kit [ab65331, l-Lactate Assay Kit (Colorimetric), Abcam] according to the manufacturer’s instructions. Fresh retinal

(14) Cancer cell-intrinsic inflammasome protein ASC links innate immunity with mitochondrial metabolism in driving pancreatic cancerNature CommunicationsFebruary 7, 2026Yu C. J. Chey, Bassam Kashgari, Louise McLeod, Georgette A. Radford, Linden J. Gearing, Ruby E. Dawson, Malvika Kharbanda, Joanne Lundy, Daniel Croagh, Charlotte Girard-Guyonvarc’h, et al.and human cell line supernatants (1:5 dilution) using the L-lactate Colorimetric Assay kit (Abcam, ab65331), according to manufacturer’s instructions. Tissue samples were lysed in 6× volume (6 µL buffer/1 mg

(15) Transcriptomic profiling of co-cultured cancer-host cells identifies hypoxia as a driver of the skeletal muscle cell's anti-proliferative effect on cancer cellsOncogenesisFebruary 7, 2026Anine Aunan, Charlotte Claeyssen, Mohamed Abdelhalim, Jérôme Ruzzinmeasurement Extracellular lactate levels were measured in culture supernatants using the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), following the manufacturer’s instructions. Extracellular glucose levels were

(16) Acute myeloid leukemia (AML)-derived bone mesenchymal stem cell exosomal METTL14 promotes AML cell growth and glycolysis by HOXA3/WNT7B axisCell Biology and ToxicologyJanuary 29, 2026Wanying Liu, Xi Ming, Jiaying Wu, Sijia Yan, Shuai Su, Rubing Zheng, Yu Wang, Yi Xiaolysates (2 × 107 cells/mL) were detected using Glucose Assay Kit (ab65333, Abcam), Lactate Assay Kit (ab65331, Abcam), and the ADP/ATP Ratio Assay kit (MAK135, Sigma-Aldrich) basing on kit instructions. Western blot (WB

(17) Genetic depletion of the early autophagy protein ATG13 impairs mitochondrial energy metabolism, augments oxidative stress, induces the polarization of macrophages to the M1 inflammatory mode, and compromises myelin integrity in skeletal muscleInflammation ResearchJanuary 27, 2026Mubaraq A Toriola, Emma Timlin, Sarojini Bulbule, Amy Reyes, Omolola Mary Adedeji, C Gunnar Gottschalk, Animesh Barua, Leggy A Arnold, Avik Roybecause the acidification took place due to lactate release. Subsequently, lactate was measured (Cat# ab65331; Abcam) in Mφs after 24 h of serum starvation. Quantitative analysis (Fig. 6L) showed that Tg Mφs released

(18) Harnessing native corticosteroid-binding globulin to treat life-threatening septic shockEndocrinologyJanuary 12, 2026Stewart D Ramsay, Declan E Kilgariff, Benjamin J Young, Luke C Darveniza, Ryan L O’Hare Doig, Courtney A Hollis, Plinio R Hurtado, Mark P Plummer, Edward G Robins, Jessica H Lee, et al.AB 3677403), albumin (#80630, Crystal Chem, RRID: AB-3677402), and tissue hypoxia marker lactate, (#AB65331, Abcam plc, Cambridge, UK), and organ damage markers of cardiac troponin-I (#LS-F4165, LS Bio Newark,

(19) Maternal milk-derived lipids dictate neonatal macrophage phenotype and functioniScienceDecember 19, 2025Lunhua Liu, Jiyeon Yang, Jiro Sakai, Ranadhir Dey, Isabelle Coppens, Hira L. Nakhasi, Mustafa AkkoyunluAb65336 Cholesterol Assay Kit - HDL and LDL/VLDL,Abcam,Cat#: Ab65390 L-Lactate Assay Kit,Abcam,Cat#: Ab65331 Glucose Uptake Assay Kit,Abcam,Cat#: Ab136955 ,, Deposited data,Deposited data,Deposited data ,, RNA seq

(20) Exercise-induced histone lactylation in monocyte-derived macrophages restores cardiac immune homeostasis and function in sepsis-induced cardiomyopathyNature CommunicationsDecember 15, 2025Shuo Sun, Chaojie Lai, Chengchen Huang, Xinrong Ren, Tingyu Zhang, Juan Zou, Yiping Tong, Qingyan Zhou, Jiangting Lu, Zhida Shen, et al.10.2. Intracellular lactate measurement Intracellular lactate levels were measured using a lactate assay kit (ab65331, Abcam), following the protocol provided by the manufacturer. Histology and immunostainings Mouse

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L-Lactate Assay Kit (Colorimetric) from Abcam

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L-Lactate Assay Kit (Colorimetric) from Abcam

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